Article
Multidisciplinary Sciences
Hongxia Song, Qiang Lu, Leiping Hou, Meilan Li
Summary: The study demonstrates that increasing CO2 concentration can enhance biomass and carotenoid content in carrots. By identifying genes related to carotenoids and verifying their activities, the molecular mechanism of this enhancement was elucidated.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Maria Doroteia Campos, Catarina Campos, Amaia Nogales, Helia Cardoso
Summary: The experiment revealed that DcAOX2a plays a dual role in carrot tap root secondary growth and cold stress response, while DcAOX1 shows less correlation. Cold stress generally up-regulated DcAOX2a without consistent effects on DcAOX1, indicating a potential key role of DcAOX2a in the response to stress. The identification of cis-acting regulatory elements at the promoters of both genes showed putative sequences involved in cold stress responsiveness and growth, with DcAOX2a promoter presenting more CAREs related to hormonal pathways than DcAOX1.
Article
Plant Sciences
Tong Li, Yuan-Jie Deng, Jie-Xia Liu, Ao-Qi Duan, Hui Liu, Ai-Sheng Xiong
Summary: This study revealed that DcCCD4 plays a significant role in the accumulation of carotenoids in carrot taproots by cleaving alpha- and beta-carotene, which affects the color of the taproot.
Article
Chemistry, Applied
Christophe Aubert, Marie Bruaut, Guillaume Chalot
Summary: This study analyzed the primary and secondary metabolites in different parts of two orange carrots, highlighting the considerable variation in levels between tissues and sections. The peel was found to have higher levels of citric acid, gamma-tocopherol, 6-methoxymellein, falcarindiol, and most polyphenols and terpenes, while lower levels of sugars, alpha-tocopherol, and falcarinol. Lutein was mainly localized in the xylem. The sucrose, carotenoid, alpha-tocopherol, and falcarindiol acetate contents decreased from the top sections to the bottom sections. Most polyphenols showed similar patterns in the peel, while malic acid showed a similar pattern in the phloem. Falcarinol content decreased in the top sections of the phloem but increased in the xylem. No clear trend was observed for gamma-tocopherol and ascorbic acid.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Plant Sciences
Josefina Wohlfeiler, Maria Soledad Alessandro, Andres Morales, Pablo Federico Cavagnaro, Claudio Romulo Galmarini
Summary: Carrots have genotype-dependent vernalization requirement, where longer cold exposures lead to increased flowering rates and earlier onset of flowering. However, post-vernalization day length does not significantly affect carrot flowering.
Article
Environmental Sciences
Samia Faiz, Anis Ali Shah, Naima Huma Naveed, Anila Nijabat, Nasim Ahmad Yasin, Aima Iram Batool, Hayssam M. Ali, Talha Javed, Phillip W. Simon, Aamir Ali
Summary: The study investigated the synergistic role of AgNPs and IAA in improving resilience against Cd toxicity in carrot plants, enhancing plant growth attributes and detoxifying reactive oxygen species. Genotypic variation for Cd tolerance in carrots was observed, with Pre breed 22 identified as a tolerant genotype with increased Cd content in roots compared to shoots.
Article
Environmental Sciences
Adnan Shakeel, Aashaq Hussain Bhat, Aadil Amin Bhat, Abrar Ahmad Khan
Summary: The application of fly ash improved the important physicochemical characteristics of soil. Carrot grown in a growth substrate containing 15% fly ash showed significantly improved plant growth, yield, and pigment content compared to untreated inoculated plants. Additionally, the proline content, superoxide dismutase (SOD), and catalase (CAT) activity were enhanced by applying 15% fly ash, suggesting its sustainable use to improve growth, yield, and resistance of carrot against M. incognita infection.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Agronomy
Robert Rosa, Larysa Hajko, Jolanta Franczuk, Anna Zaniewicz-Bajkowska, Alena Andrejiova, Ivana Mezeyova
Summary: Amino acids used as plant stimulants have a positive impact on crop quality and yields, playing a crucial role in global food security. This study investigated the effects of two amino acids, L-tryptophan and L-glutamic acid, on carrot yield and quality, and found that the synergistic action of these two amino acids significantly increased carrot yield and improved protein and total soluble solids content.
Article
Agronomy
Abdul Hameed, Nudrat Aisha Akram, Muhammad Hamzah Saleem, Muhammad Ashraf, Shakeel Ahmed, Shafaqat Ali, Abdulaziz Abdullah Alsahli, Mohammed Nasser Alyemeni
Summary: The influence of seed priming with different concentrations of alpha-tocopherol on carrot plants under water-deficit conditions was investigated. The application of alpha-tocopherol was effective in improving plant growth attributes and oxidative defense system, especially in reducing the accumulation of H2O2 and MDA contents. Additionally, cv.DC4 showed better performance in growth attributes.
Article
Chemistry, Multidisciplinary
Laura Sofia Santoscoy-Berber, Marilena Antunes-Ricardo, Melissa Zulahi Gallegos-Granados, Juan Carlos Garcia-Ramos, Alexey Pestryakov, Yanis Toledano-Magana, Nina Bogdanchikova, Rocio Alejandra Chavez-Santoscoy
Summary: The study showed that the use of silver nanoparticles significantly increased the antioxidant capacity of carrots and also affected the content of specific phenolic compounds.
Article
Biochemistry & Molecular Biology
Niyaz A. Dar, Mudasir A. Mir, Javid Mir, Sheikh Mansoor, Wasia Showkat, Tasmeen J. Parihar, Syed Anam Ul Haq, Shabir H. Wani, Gul Zaffar, Khalid Z. Masoodi
Summary: This study analyzed the effect of cold stress on the expression of major anthocyanins and anthocyanin biosynthetic pathway genes in black carrots. The results showed that cold stress increased the accumulation of major anthocyanins and was associated with increased expression of the MYB6 and LDOX1 genes.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Nutrition & Dietetics
Mikayla S. Kaeppler, Jordan B. Smith, Christopher R. Davis, Philipp W. Simon, Sherry A. Tanumihardjo
Summary: Two studies on weanling male Mongolian gerbils examined the relative bioefficacy of non-provitamin A carotenoids (PAC) - lycopene or anthocyanins from multicolored carrots - to vitamin A (VA). The results showed that simultaneous consumption of these phytochemicals did not affect the relative bioefficacy of BCE.
JOURNAL OF NUTRITION
(2023)
Article
Biology
Natalia Shmykova, Elena Domblides, Tatiana Vjurtts, Arthur Domblides
Summary: The process of embryogenesis in isolated microspore culture of eight different carrot accessions was studied, showing differences in responsiveness. Embryogenesis was induced using specific medium and temperature treatments, resulting in the observation of initial embryoid formation, division, and regeneration depending on the genotype. The study demonstrated the potential for secondary embryoid production and revealed variations in embryogenic abilities among the carrot accessions.
Article
Agriculture, Multidisciplinary
Amy Schmiedeskamp, Monika Schreiner, Susanne Baldermann
Summary: This study investigated the potential to increase carotenoid contents in food by choosing food with naturally high carotenoid contents and utilizing thermal processing methods that maintain their stability. Results showed significant variations in carotenoid contents among different colored carrot cultivars, and air frying and deep frying processes led to a significant decrease in total carotenoid contents.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Raman Selvakumar, Pritam Kalia, Dalasanuru Chandregowda Manjunathagowda, R. S. Raje
Summary: The results of the inheritance study showed that the color differences between purple and yellow carrot roots are controlled by single dominant genes. Flavonoid analysis revealed different compounds in carrots of different colors, and grey-purple roots had the highest levels of beta-carotene and lutein. Molecular markers and genetic mapping can be used for the selection and breeding of carrots with improved nutritional content.
GENETIC RESOURCES AND CROP EVOLUTION
(2022)
Article
Plant Sciences
Hao Wang, Jie-Xia Liu, Kai Feng, Tong Li, Ao-Qi Duan, Yan-Hua Liu, Hui Liu, Ai-Sheng Xiong
Summary: The study identified that the overexpression of AgMYB12 in celery can enhance the accumulation of apigenin by interacting with the AgFNS gene, suggesting AgMYB12 as a positive regulator of apigenin biosynthesis.
PLANT CELL REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mengyao Li, Jie Li, Ran Zhang, Yuanxiu Lin, Aisheng Xiong, Guofei Tan, Ya Luo, Yong Zhang, Qing Chen, Yan Wang, Yunting Zhang, Xiaorong Wang, Haoru Tang
Summary: This study investigated the physiological mechanisms of celery in response to high temperatures by examining its morphological and physiological indices. Results showed that under high-temperature conditions, antioxidant enzyme activity, proline, relative conductivity, and malondialdehyde increased, while chlorophyll and leaf water content decreased. Short-term heat treatment resulted in increased stomatal conductance to cool off the leaves, while long-term heat treatment led to stomatal closure to prevent leaf dehydration. Metabolomic and transcriptomic analyses revealed the regulatory mechanisms at the molecular level in celery's response to heat stress. The study identified important metabolites and genes involved in the response to high temperatures in celery, providing a theoretical basis for breeding heat-resistant celery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Feng Que, Qingnan Liu, Ruofei Zha, Aisheng Xiong, Qiang Wei
Summary: The TALE gene family in bamboo has undergone duplication and expansion, showing high synteny with TALE genes in rice. PhTALEs have been found to play a role in regulating secondary cell wall development during the fast-growing stage of moso bamboo. This expansion of the TALE gene family may be associated with increased lignification in woody bamboo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Horticulture
Shan-Shan Tan, Min Jia, Xin-Yue Zhang, Ao-Qi Duan, Tong Li, Yan-Hua Liu, Ai-Sheng Xiong
Summary: The germination process of carrot seeds is regulated by phytohormones, and this study provides new insights into the relationship between phytohormones and carrot seed germination through morphological observation, transcriptome sequencing, and gene expression analysis.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Min Jia, Sheng-Qi Zhu, Ya-Hui Wang, Jie-Xia Liu, Shan-Shan Tan, Hui Liu, Sheng Shu, Jian-Ping Tao, Ai-Sheng Xiong
Summary: This study investigated the effects of different storage conditions on celery morphological characteristics, anatomical features, and antioxidant accumulation. The results showed that storing celery in darkness at low temperature was the optimal condition for maintaining its morphological characteristics and preserving antioxidant accumulation. Additionally, the genes GDP-d-mannose pyrophosphorylase and l-galactose dehydrogenase were found to play a crucial role in the accumulation of ascorbic acid in celery.
Article
Horticulture
Shan-Shan Tan, Ao-Qi Duan, Guang-Long Wang, Hui Liu, Zhi-Sheng Xu, Ai-Sheng Xiong
Summary: This study conducted a genome-wide identification and characteristic analysis of MADS-box transcription factors in carrots, and identified many important transcription factors involved in the nutritional and reproductive growth of plants. The results of subcellular localization, gene distribution, and protein interaction provide a theoretical basis for exploring the molecular mechanisms of carrot flower organ development.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Ao-Qi Duan, Yuan-Jie Deng, Shan-Shan Tan, Zhi-Sheng Xu, Ai-Sheng Xiong
Summary: The study identified the DcMYB11c gene as a key factor in anthocyanin pigmentation in purple petioles of carrots. Overexpression of DcMYB11c in orange and yellow taproot carrots led to deep purple pigmentation, while knockout of DcMYB11c in purple taproot carrots resulted in pale purple pigmentation. DcMYB11c was found to activate the expression of genes responsible for anthocyanin biosynthesis and glycosylation/acetylation. This research provides new insights into the regulation of anthocyanin biosynthesis in carrots and may have implications for similar studies in other plants.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Genetics & Heredity
Zhipeng Zhu, Jianxiang Yu, Xinhui Tang, Aisheng Xiong, Miao Sun
Summary: In this study, 18 potential reference genes from okra were selected and their expression levels under different tissues and abiotic stress conditions were determined using Real-Time quantitative PCR. The most stable genes under cold, salt and drought stress were identified, providing a molecular basis for functional gene analysis and regulatory mechanism research of okra.
Article
Agronomy
Qian Jiang, Yu Wang, Aisheng Xiong, Hui Zhao, Ruizong Jia, Mengyao Li, Huaming An, Changmian Ji, Anping Guo
Summary: By analyzing the papaya genome and large-scale RNA-seq data, we found that the formation of the NLR gene family in papaya is mainly driven by duplication and these genes play a vital role in biotic stress responses.
Review
Agronomy
Jian Zhang, Weikai Fang, Chidong Xu, Aisheng Xiong, Michael Zhang, Randy Goebel, Guangyu Bo
Summary: Advances in optical sensing technology have revolutionized the determination of crop seed vigor. Traditional methods are time-consuming, labor-intensive, and prone to inaccuracies, leading to unreliable yield predictions. Optical sensing technology offers rapid, accurate, and non-destructive calibration methods for seed usage and agronomic evaluation. This review provides a summary of current research in using optical sensing technology for assessing seed vigor.
Article
Biochemistry & Molecular Biology
Xiaoyan Li, Mengyao Li, Weilong Li, Jin Zhou, Qiuju Han, Wei Lu, Qin Luo, Shunhua Zhu, Aisheng Xiong, Guofei Tan, Yangxia Zheng
Summary: This study identified the mitochondrial genomes of Apium graveolens and Apium leptophyllum using different sequencing platforms. It revealed specific gene coding preferences, RNA editing types, and homologous sequences in these two species. These findings provide important data for genetic and evolutionary research in the Apium genus, as well as for comparative genomic analysis in other species within the Apiaceae family.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Jiageng Du, Huitao Zhang, Weilong Li, Xiaoyan Li, Zhuo Wang, Ying Zhang, Aisheng Xiong, Mengyao Li
Summary: This study focused on the isolation and transformation of celery protoplasts using celery seedling leaves as the primary materials. The most effective procedure for protoplast isolation was enzymatic digestion followed by filtration and centrifugation. The study also established a transient transformation system and used it to analyze the protein localization characteristics of the celery transcription factor AgMYB80. The findings confirmed the reliability and effectiveness of the transient transformation system.
Article
Horticulture
Rong-Rong Zhang, Jian-Ping Tao, Liu-Xia Song, Jia-Qi Zhang, Hui Liu, Wei-Min Zhu, Tong-Min Zhao, Ai-Sheng Xiong
Summary: Tomato is a widely cultivated vegetable with nutritious fruits. Soluble solids content (SSC) plays a decisive role in improving tomato quality. The molecular marker TGS0892 on chromosome 6 of tomato genome is closely related to soluble solids. In this study, bioinformatics methods were used to identify and analyze genes near TGS0892. The expression levels of these genes in different tomato cultivars showed variations, and two genes, Soly065970 and Soly066010, were found to be involved in regulating the soluble solids metabolic process.
Article
Agronomy
Guang-Long Wang, Jia-Qi Wu, Yang-Yang Chen, Yu-Jie Xu, Cheng-Ling Zhou, Zhen-Zhu Hu, Xu-Qin Ren, Ai-Sheng Xiong
Summary: This article focuses on the synthesis, accumulation, and regulation of lignin in horticultural crops in recent years, and provides insights into its impact on the quality of horticultural products. The importance of controlling lignin content is highlighted, and new directions for further research on lignin metabolism are proposed.
Article
Plant Sciences
Ao-Qi Duan, Yuan-Jie Deng, Shan-Shan Tan, Shan-Shan Liu, Hui Liu, Zhi-Sheng Xu, Sheng Shu, Ai-Sheng Xiong
Summary: This study investigates the molecular mechanism of purple carrot taproot color formation and successfully knocks out the key gene DcGST1, resulting in carrots with orange taproots. Furthermore, it is found that the anthocyanin activator DcMYB7 can activate the expression of DcGST1, and co-expression of DcGST1 and DcMYB7 significantly increases anthocyanin accumulation in carrot calli.
