Article
Biochemistry & Molecular Biology
Haixiao Hu, Yujie Meng, Wenxin Liu, Shaojiang Chen, Daniel. E. E. Runcie
Summary: Recent advances in maize DH technology enable the efficient development of large numbers of DH lines. Phenotyping of haploid progenitors can accelerate the selection of DH lines. Multi-trait models improve genomic prediction accuracy for DH lines, but incorporating information from haploid lines does not further improve the prediction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Katarzyna Juzon, Marzena Warchol, Kinga Dziurka, Ilona Mieczyslawa Czyczylo-Mysza, Izabela Marcinska, Edyta Skrzypek
Summary: This study investigated the influence of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration on doubled haploid (DH) production. It was found that a higher concentration of 2,4-D can enhance the formation of haploid plants and seeds, but strong genotypic variation may limit the development of effective breeding methods.
Article
Agronomy
Jian Wang, Yuexuan Cao, Kejian Wang, Chaolei Liu
Summary: The panel of multiple-heading-date mtl haploid inducer lines developed in this study can address the issue of asynchronous heading dates between haploid inducers and pollen acceptors, accelerating the use of in vivo DH technology in rice breeding.
Article
Genetics & Heredity
Muhammad Massub Tehseen, Fatma Aykut Tonk, Muzaffer Tosun, Harpinder Singh Randhawa, Ezgi Kurtulus, Izzet Ozseven, Behyan Akin, Ozge Nur Zulfuagaoglu, Kumarse Nazari
Summary: This study identified multiple QTLs controlling stripe rust resistance in wheat using an elite line with adult plant resistance. SNP markers flanking the QTL regions can aid in marker-assisted selection.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Xinyi Zhou, Wei Xie, Hao Jing, Junru Fu, Maomao Li, Jianmin Bian, Jie Xu, Yicong Cai, Haohua He, Dahu Zhou
Summary: This study used a functional rice variety, Zibaoxiangnuo 1 (ZBXN 1), rich in total flavonoids and anthocyanins, as the experimental material, and crossed it with Minghui63 (MH63), a variety without anthocyanins, to construct Recombination Inbred Lines (RILs). The contents of anthocyanins and total flavonoids in RILs and two parents were determined for three consecutive generations. The results showed that ZBXN 1 had abundant and stable anthocyanins, making it a potential breeding material for high-quality rice varieties with high anthocyanin content.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Harjot Kaur, Mohammed Kyum, Surinder Sandhu, Gagandeep Singh, Priti Sharma
Summary: The optimized protocol using CIMMYT haploid inducer CIM2GTAIL P2 significantly improves the haploid induction rate and survival rate in sub-tropical maize, accelerating the hybrid breeding program and reducing the production cost of doubled haploid.
Article
Horticulture
Miki Kawano, Masaki Yahata, Tokurou Shimizu, Chitose Honsho, Tomonari Hirano, Hisato Kunitake
Summary: Genetic analysis was carried out on five haploid plants of 'Banpeiyu' pummelo using SSR markers to confirm their gynogenetic origin. Results confirmed their origin and revealed distorted segregation ratios in certain SSR markers during self-pollination and reciprocal crosses experiments. This study provides valuable insights into the genetic characteristics of haploid and doubled haploid plants in fruit crops.
SCIENTIA HORTICULTURAE
(2021)
Article
Agronomy
Saksham Pundir, Vikas Kumar Singh, Satish Kumar, Deepti Chaturvedi, Deepak Kumar, Rambir Singh Kanwar, Ashok Kumar, Andreas Boerner, Shiveta Sharma, Shailendra Sharma
Summary: In this study, a major QTL associated with the resistance to the cereal cyst nematode Heterodera avenae was identified in wheat. The resistant DH lines showed a decrease in the number of nematode juveniles with no presence of J4 stage juveniles compared to the susceptible lines. In addition, the resistant DH lines exhibited higher biological yield, grain yield, and harvest index compared to the known resistant parent over a span of two years. These findings suggest that the selected DH lines are potential pre-breeding materials to introduce novel genetic variations for H. avenae resistance in cultivated wheat.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Agronomy
Vikrant Khare, Rama Shankar Shukla, Sanjay Kumar Singh, Suneeta Pandey
Summary: This study explores the variation and recognition of heat responsive traits in wheat, aiming to enhance wheat productivity in high temperature stress conditions. The findings provide valuable insights into the key factors influencing grain yield and offer a framework for developing heat tolerant cultivars in the future.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2024)
Article
Agronomy
Iara Goncalves dos Santos, Anderson Luiz Verzegnazzi, Jode Edwards, Ursula K. Frei, Nicholas Boerman, Leandro Tonello Zuffo, Luiz P. M. Pires, Gerald de la Fuente, Thomas Lubberstedt
Summary: This study demonstrates that spontaneous haploid genome doubling does not affect the performance of inbred lines and that BS39 genes have the potential to add novel alleles to temperate elite germplasm.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Surbhi Grewal, Veronica Guwela, Claire Newell, Cai-yun Yang, Stephen Ashling, Duncan Scholefield, Stella Hubbart-Edwards, Amanda Burridge, Alex Stride, Ian P. King, Julie King
Summary: This study generated 86 stable inherited doubled haploid (DH) wheat-T. urartu introgression lines, which were characterized using genotyping arrays and KASP markers to identify unique introgressions in a bread wheat background. The DH lines have accelerated the breeding process and enabled the early release of homozygous wheat-T. urartu introgression lines, which can greatly advance the identification of beneficial alleles for wheat improvement.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Ana Lopez-Malvar, Zoila Resendiz, Rogelio Santiago, Jose Cruz Jimenez-Galindo, Rosa Ana Malvar
Summary: Corn borers are the most important pest affecting maize, and resistance to them can compromise plant fitness and yield. Stronger resistant maize lines may exhibit higher concentrations of cell wall-bound hydroxycinnamates, but also characteristics like early maturity, low grain moisture, and reduced plant height. Selecting for resistance may therefore have negative impacts on yield. A breeding strategy focusing on grain yield to tolerate corn borer attack is recommended.
Article
Plant Sciences
Fahad Ali, Wei Chen, Sajid Faiz, Yakun Wang, Xiangjin Wei, Lihong Xie, Guiai Jiao, Gaoneng Shao, Shikai Hu, Shaoqing Tang, Zhonghua Sheng, Peisong Hu
Summary: In this study, QTL analysis was performed to identify stable genes related to rice grain appearance quality. GL3, GW3, LWR3 and TGW3 were found to be responsible for grain length and grain width. Additionally, 8 QTLs related to additive environment interaction were identified. These findings are valuable for future map-based cloning and improvement of grain appearance quality and yield.
PAKISTAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Alejandro Ledesma, Fernando Augusto Sales Ribeiro, Alison Uberti, Jode Edwards, Sarah Hearne, Ursula Frei, Thomas Lubberstedt
Summary: The molecular characterization analysis confirmed the loss of genetic variability from C0 to C17 through the recurrent selection process in maize breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Jahad Soorni, Zahra-Sadat Shobbar, Danial Kahrizi, Federica Zanetti, Kaveh Sadeghi, Sara Rostampour, Peter Gergo Kovacs, Attila Kiss, Iman Mirmazloum
Summary: This study investigated the diverse agronomic traits of 136 doubled haploid (DH) camelina lines, including seed yield, fatty acid composition, and protein content. The results showed significant diversity among the lines for these traits. The positive relationships observed suggest the potential for developing higher-yielding camelina cultivars with high seed oil content. The study also identified promising genotypes for further breeding to increase seed yield and improve food/nonfood traits.
Article
Medicine, General & Internal
Xinyu Cai, Yue Jiang, Zhiwen Cao, Mei Zhang, Na Kong, Lina Yu, Yedong Tang, Shuangbo Kong, Wenbo Deng, Haibin Wang, Jianxin Sun, Lijun Ding, Ruiwei Jiang, Haixiang Sun, Guijun Yan
Summary: The expression of Mammalian sterile 20 (STE20)-like kinase 1 (Mst1) in the endometrium was decreased in women with recurrent implantation failure (RIF), but its activation promoted trophoblast-uterine epithelium adhesion. Nur77 facilitated trophoblast-uterine epithelium adhesion through the Mst1 signaling pathway and regulated embryo implantation by modulating integrin signaling. This study provides a new approach for assessing endometrial receptivity and treating implantation failure.
Article
Plant Sciences
Rudra Bhattarai, Hui Liu, Kadambot H. M. Siddique, Guijun Yan
Summary: This study compared two NIL pairs with the most contrasting performance in metribuzin treatment and different genetic backgrounds using RNA sequence analysis, identifying nine candidate genes underlying Qsns.uwa.4 A.2 responsible for metribuzin resistance. Quantitative RT-qPCR further validated the candidate genes, with TraesCS4A03G1099000 (nitrate excretion transporter), TraesCS4A03G1181300 (aspartyl protease), and TraesCS4A03G0741300 (glycine-rich proteins) identified as key factors for metribuzin resistance.
Article
Genetics & Heredity
Shanwu Lyu, Qiming Mei, Hui Liu, Baosheng Wang, Jun Wang, Hans Lambers, Zhengfeng Wang, Bin Dong, Zhanfeng Liu, Shulin Deng
Summary: This study presents a high-quality genome assembly of Plantago major L., a species of Plantago, using Oxford Nanopore sequencing and Hi-C technologies. The genome size was approximately 671.27 Mb with a contig N50 length of 31.30 Mb, and 31,654 protein-coding genes were identified. Evolutionary analysis revealed divergence of P. major from other Lamiales species about 62.18 Mya and two rounds of WGD events. The expansion of gene families related to plant acclimation and adaptation, as well as the high expression patterns of polyphenol biosynthesis genes in roots, were observed. The decoding of P. major genome provides valuable genomic resources for various research areas.
Article
Plant Sciences
Guangcan Yu, Jing Chen, Mengxiao Yu, Andi Li, Ying-Ping Wang, Xinhua He, Xuli Tang, Hui Liu, Jun Jiang, Jiangming Mo, Shuo Zhang, Junhua Yan, Mianhai Zheng
Summary: Nitrogen deposition does not increase plant phosphorus demand in a nitrogen-saturated mature tropical forest. Different nitrogen addition rates regulate soil phosphorus transformation through microbial community transition.
JOURNAL OF ECOLOGY
(2023)
Article
Plant Sciences
Sina Nouraei, Md Sultan Mia, Hui Liu, Neil C. C. Turner, Javed M. M. Khan, Guijun Yan
Summary: Drought is a significant challenge for wheat production and this study investigated the mechanism behind drought tolerance in wheat using proteomic analysis. The study identified 41 differentially expressed proteins related to drought tolerance and revealed important pathways involved in drought tolerance, such as transcription, translation, protein export, photosynthesis, and carbohydrate metabolism. Several candidate proteins responsible for drought tolerance were suggested, including 30S ribosomal protein S15, SRP54 domain-containing protein, auxin-repressed protein, serine hydroxymethyltransferase, and an uncharacterized protein.
Article
Plant Sciences
Tanushree Halder, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Identifying quantitative trait loci (QTL) for different root traits in bread wheat can help breeders develop climate-resilient and high-yielding wheat varieties. This study detected 14 QTL for eight root traits on nine wheat chromosomes and validated two QTL for shallow root depth and high root dry mass in independent populations. The identified QTL and candidate genes are valuable resources for future root trait improvement in wheat.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Endocrinology & Metabolism
Qingqing Shi, Chenyang Huang, Jingyu Liu, Yifan Li, Na Kong, Jie Mei, Xiaoyue Shen, Yanxin Sun, Feifei Lu, Haixiang Sun, Guijun Yan
Summary: Investigated the effects of hormone replacement therapy (HRT) alone or in combination with tamoxifen on patients with thin endometrium in frozen-thawed embryo transfer (FET) cycles. Found that while tamoxifen can significantly increase endometrial thickness, it may not improve the pregnancy outcomes of patients with thin endometrium.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zishu Xu, Yi Duan, Hui Liu, Mingchao Xu, Zhi Zhang, Ling Xu
Summary: The study identified and molecularly evolved the WAK gene family in Cruciferous species. A total of 178 WAK genes were identified, and a phylogenetic tree showed that Brassica rapa, Brassica oleracea, and Brassica napus genes were closely related. The WAK gene family was unevenly distributed in B. napus chromosomes, with the largest number of genes on chromosome C08. Expression analysis revealed variations in the expression patterns of the WAK gene family under different stress treatments. This study lays the foundation for further understanding the functional mechanisms of the WAK gene family in Brassica napus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Agronomy
Guannan Liu, Dongcheng Liu, Aimin Zhang, Hui Liu, Md Sultan Mia, Daniel Mullan, Guijun Yan
Summary: This study identified a novel SNP and developed a highly efficient KASP marker for drought tolerance in wheat. The findings have significant implications for research on drought tolerance in wheat.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Agronomy
Guannan Liu, Daniel Mullan, Aimin Zhang, Hui Liu, Dongcheng Liu, Guijun Yan
Summary: Common wheat is an important crop, but markers and genes related to pre-harvest sprouting (PHS) resistance in white-grained wheat are limited. In this study, KASP markers and functional genes related to PHS resistance on wheat chromosome 3AL were identified using near isogenic lines and the 90K SNP chip. These markers and genes are valuable for high-throughput evaluation, marker-assisted selection, and understanding the genetic pathways controlling PHS.
Article
Microbiology
Zishu Xu, Hui Liu, Najeeb Ullah, Shahbaz Atta Tung, Basharat Ali, Xin Li, Shubin Chen, Ling Xu
Summary: This study compared the rhizosphere microbial communities of Salvia miltiorrhiza and S. castanea, revealing differential structures at different times. It also investigated the impact of altitude and soil moisture on the distribution of S. castanea. These findings enhance our understanding of plant adaptation to geographical environments and provide a theoretical basis for the cultivation of S. miltiorrhiza and the use of S. castanea resources.
FEMS MICROBIOLOGY LETTERS
(2023)