Article
Agronomy
Shuai Liu, Dinghao Lv, Chan Lu, Yaping Xiao, Shiqiang Wang, Wen Zhou, Junfeng Niu, Zhezhi Wang
Summary: The study reveals that Tulasnella fungi from the mycorrhiza of B. striata can promote seed germination and seedling growth by producing plant hormones.
Article
Microbiology
Yi-Hua Wu, De-Yun Chen, Xin-Ju Wang, Neng-Qi Li, Jiang-Yun Gao
Summary: The study developed a method using ex situ seedling baiting to capture seedling-associated fungi from Dendrobium officinale. The results suggest that this approach is an easy and efficient way to obtain fungi for this species.
Review
Plant Sciences
Da-Ke Zhao, Marc-Andre Selosse, Limin Wu, Yan Luo, Shi-Cheng Shao, Yong-Ling Ruan
Summary: The article discusses the endangered status of orchids in the plant kingdom and proposes a new reintroduction method for orchids by co-sowing orchid seeds directly with orchid mycorrhizal fungi carrying ecological specificity. This innovative approach aims to reconstruct long-term and self-sustained orchid populations, representing a potential benefit for the reintroduction of endangered orchids in nature.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yuan-Yuan Li, Margaux Boeraeve, Yu-Hsiu Cho, Hans Jacquemyn, Yung- Lee
Summary: Mycorrhizal associations play a crucial role in orchid germination and seedling establishment, potentially impacting the distribution and abundance of orchids in natural conditions. The mycoheterotrophic orchid Gastrodia confusoides displays random aboveground spatial patterns of distribution within bamboo forests, possibly due to the scattered distribution of litter-decaying fungi. Our study provides evidence that the abundance of litter-decaying fungi varies randomly within the bamboo forest and independently from G. confusoides adults.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Xiang-Gui Chen, Yi-Hua Wu, Neng-Qi Li, Jiang-Yun Gao
Summary: Compatible fungi can promote the germination and seedling formation of Dendrobium officinale seeds, while incompatible fungi cannot continuously colonize seeds and support seedling development. Seed pretreatment can improve seed germination, but cannot change the compatibility of a fungus with an orchid. Without a seed coat, the incompatible fungus is still unable to colonize in vitro-produced protocorms or support seedling development.
Article
Horticulture
Yanyan Gao, Sijing Peng, Ye Hang, Guofang Xie, Ning Ji, Mingsheng Zhang
Summary: Research has shown that the isolated strain Coprinellus disseminatus DJF-10 from the roots of Cremastra appendiculata can promote seed germination and induce seedling growth, providing new support for orchid seed germination and seedling development.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Fan Tian, Xiao-Feng Liao, Lian-Hui Wang, Xin-Xiang Bai, Yan-Bin Yang, Zai-Qi Luo, Feng-Xia Yan
Summary: Mycorrhizal fungi play a crucial role in seed germination and seedling development of Paphiopedilum barbigerum, with Epulorhiza sp. FQXY019 showing significant promotion in seed germination and seedling growth. Inoculation with FQXY019 leads to progression from seed germination to rooting stage, with different effects observed in different culture media.
PLANT SIGNALING & BEHAVIOR
(2022)
Article
Biodiversity Conservation
Xin-Ju Wang, Yi-Hua Wu, Xing-Jia Ming, Gang Wang, Jiang-Yun Gao
Summary: This study developed an easy-to-use solution by isolating fungi and using fungus-seed bags to promote protocorm formation and seedling development in D. officinale, providing an effective method for restoration-friendly cultivation of this critically endangered orchid. The fungus-seed bags showed advantages such as low-cost mass production, convenience in transportation, and environmentally-friendly materials, making it a universal application for conservation translocations of epiphytic orchids based on symbiotic seeds germination.
GLOBAL ECOLOGY AND CONSERVATION
(2021)
Article
Plant Sciences
Liz Koziol, Thomas P. McKenna, James D. Bever
Summary: This study investigated the effect of microbial communities on seedling biomass and diversity by seeding pots with native prairie seeds and a non-native competitor. The results showed that inoculating soil with prairie arbuscular mycorrhizal fungi and ex-arable soil increased abundance and diversity of native and late successional plants, leading to decreased abundance of the non-native grass S. faberi.
Article
Plant Sciences
Tomas Figura, Edita Tylova, Jana Jersakova, Martin Vohnik, Jan Ponert
Summary: Many orchid species are threatened by factors like eutrophication and nitrate, which can suppress non-symbiotic orchid seed germination. The early development of orchids depends on mycorrhizal symbiosis, and the presence of compatible mycobionts is crucial for successful seed germination in the presence of nitrate.
Article
Microbiology
Marc Freestone, Celeste Linde, Nigel Swarts, Noushka Reiter
Summary: Understanding the nutrient requirements of orchid mycorrhizal fungi (OMF) is crucial for the conservation of threatened orchids and their symbiotic relationships. This study investigated how different nutrient media and fungal isolates affected the germination of Prasophyllum frenchii. The results showed that both the media and fungal isolates had a significant influence on germination percentage. Additionally, there was variation in aerial hyphal growth behavior among different OMF isolates. These findings highlight the importance of fungal diversity within the same operational taxonomic unit (OTU) and have implications for ecological research and conservation.
Article
Biodiversity Conservation
Zachary R. Wakefield, Andre R. O. Cavalcanti, Lucia Driessen, Ana Jaramillo, Edward J. Crane, Giuliano Richetta, Wallace M. Meyer
Summary: In this study, the effects of invasive mustard (H. incana) on soil microbial assemblages were examined. It was found that mustard invasions reduce soil microbial abundances by over 50% and alter soil fungal assemblages. Furthermore, low mustard germination in CSS soils supports the hypothesis of biological resistance to mustard invasion in CSS.
Article
Plant Sciences
Promila Pathak, Anamika Kumari, Brent D. Chandler, Lawrence W. Zettler
Summary: In this study, the in vitro propagation of the endangered and medicinally important orchid Vanda cristata Wall. ex Lindl. was investigated using seed germination. The addition of Kinetin in the culture medium promoted successful germination, early protocorm formation, and complete seedling development. The use of Indole-3-acetic acid+6-benzyl aminopurine in the medium showed a synergistic effect. However, the phytochemical analysis revealed that the mother plant had a higher yield of secondary metabolites compared to the in vitro regenerated plants. GC-MS analysis identified 54 different phytochemicals in the leaf methanolic extracts. The findings of this study have significant implications for germplasm conservation and commercial cultivation of V. cristata for therapeutic purposes.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Tao Wang, Xiaolu Cao, Xiaojing Wang, Miao Chi, Lubin Li, Na Yao
Summary: This study systematically explored optimal reference genes for gene expression studies during the colonization of orchid seeds by the mycorrhizal fungus Tulasnella, finding that EF2, UBC, and PP2A genes showed the potential to be suitable for RT-qPCR normalization with stable expression levels.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Fan Tian, Jun-Cai Wang, Xin-Xiang Bai, Yan-Bing Yang, Lang Huang, Xiao-Feng Liao
Summary: Similar to other orchid species, Paphiopedilum hirsutissimum relies on mycorrhizal fungi for seed germination and seedling development. This study identified a specific strain of endophytic fungus that supports the advanced developmental stage of P. hirsutissimum seedlings. The findings have ecological value and may contribute to the protection and reintroduction of endangered orchid species.
PLANT SIGNALING & BEHAVIOR
(2023)