Article
Multidisciplinary Sciences
Ma. Florencia Peralta, Ma. Laura Guzman, Ma. Estefania Bracamonte, J. Diego Marco, Ma. Eugenia Olivera, Dolores C. Carrer, Paola A. Barroso
Summary: Topical application of risedronate and EuE-Ris complexes demonstrates promising efficacy in treating cutaneous leishmaniasis, showing reduction in ulcer size, promotion of healing, and significant parasitic inhibition of 69.5% to 73.7%, with a potential trend towards cure. Further enhancements of the system are needed for complete parasite elimination.
Article
Immunology
Alonso da Silva Lira Filho, Emanuella Francisco Fajardo, Kwang Poo Chang, Pauline Clement, Martin Olivier
Summary: This study focuses on the role of GP63 in Leishmania spp.-derived exosomes/extracellular vesicles (EVs/LeishEXO) in modulating immune responses and exacerbating cutaneous leishmaniasis. The researchers confirmed the specificity of a novel model by analyzing the protein composition of the extracted EVs and found that GP63 was the primary virulence factor altered in the composition. They also demonstrated that EVs with different levels of GP63 cargo had distinct immunomodulatory capabilities and impact on the cutaneous pathology of Leishmania spp. infection. These findings provide new insights into the immune response and host-pathogen interaction in cutaneous leishmaniasis and have implications for the development of new treatments and vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Parasitology
Taylon Felipe Silva, Fernanda Tomiotto-Pellissier, Aline Kuhn Sbruzzi Pasquali, Fernanda Pinto-Ferreira, Wander Rogerio Pavanelli, Ivete Conchon-Costa, Italmar Teodorico Navarro, Eloiza Teles Caldart
Summary: The study revealed that the exact mechanism leading to different clinical manifestations among strains of the same species is still uncertain, and future studies are needed to better elucidate this phenomenon.
Article
Biophysics
Daniela Coelho, Beatriz Veleirinho, Leticia Mazzarino, Thais Alberti, Elizandra Buzanello, Regina Eva Oliveira, Rosendo Augusto Yunes, Milene Moraes, Mario Steindel, Marcelo Maraschin
Summary: Cutaneous leishmaniasis is a global public health issue, and the use of synthetic chalcones shows promise in developing molecules with anti-leishmaniasis activity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Pharmacology & Pharmacy
Vagner T. de Queiroz, Bianca de O. Botelho, Natalia A. Guedes, Diana C. Cubides-Roman, Francisco de P. Careta, Jair C. C. Freitas, Daniel F. Cipriano, Adilson V. Costa, Angelo de Fatima, Sergio A. Fernandes
Summary: In this study, an inclusion complex (IC6HKTZ) of ketoconazole (KTZ) with p-sulfonic acid calix[6]arene (CX6SO3H) was prepared to improve the solubility and efficacy of KTZ against Leishmania amazonensis and Leishmania infantum promastigotes. The solubility enhancement of KTZ by 120.00 mu M CX6SO3H was about 95 times. IC6HKTZ exhibited lower IC50 values compared to free KTZ for both Leishmania species, indicating improved anti-leishmanial activities.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Microbiology
Brenda Furtado Costa, Tarcisio Navegante de Queiroz Filho, Adeniele Lopes da Cruz Carneiro, Aline Semblano Carreira Falcao, Maria Sueli da Silva Kataoka, Joao de Jesus Viana Pinheiro, Ana Paula Drummond Rodrigues
Summary: This study examines the presence and activity of MMP-2 and MMP-9 in Leishmania parasites, particularly in two different Leishmania species, L. amazonensis and L. braziliensis. The results show that MMP-9 has significantly higher gelatinolytic activity in L. amazonensis compared to L. braziliensis. The study suggests that MMP-2 and MMP-9 may play a role in the infection dynamics of these Leishmania species and could be potential markers for severe leishmaniasis and therapeutic targets.
Article
Pharmacology & Pharmacy
Patricia de A. Machado, Pollyanna S. Gomes, Monique P. D. Carneiro, Victor Midlej, Elaine S. Coimbra, Herbert L. de Matos Guedes
Summary: This study demonstrates the importance of serine proteases in Leishmania and evaluates the antileishmanial effects of a serine protease inhibitor, TPCK. TPCK shows selectivity for the parasite and has significant activity against both promastigote and intracellular amastigote forms. The treated parasites exhibit various alterations, suggesting potential mechanisms contributing to their death. In vivo studies show that TPCK reduces lesion size and parasite loads, highlighting its potential as a therapeutic target for leishmaniasis.
Article
Immunology
Marco Antonio Cabrera Gonzalez, Ana Alice Maia Goncalves, Jennifer Ottino, Jaqueline Costa Leite, Lucilene Aparecida Resende, Otoni Alves Melo-Junior, Patricia Silveira, Mariana Santos Cardoso, Ricardo Toshio Fujiwara, Lilian Lacerda Bueno, Renato Lima Santos, Tatiane Furtado de Carvalho, Giani Martins Garcia, Paulo Ricardo de Oliveira Paes, Alexsandro Sobreira Galdino, Miguel Angel Chavez-Fumagalli, Marilia Martins Melo, Denise Silveira-Lemos, Olindo Assis Martins-Filho, Walderez Ornelas Dutra, Vanessa Carla Furtado Mosqueira, Rodolfo Cordeiro Giunchetti
Summary: Visceral leishmaniasis (VL) is a fatal disease caused by Leishmania infantum, and dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) is important in controlling human and CVL infections by reducing L. infantum infection. This study evaluated the potential of two polymeric nanoformulations loaded with Leishmania amazonensis antigens as a VL vaccine using golden hamsters as a model. The results showed that the vaccines had no adverse reactions and significantly reduced the parasitic load in the liver and spleen. These vaccines may be promising candidates for a VL vaccine.
Article
Immunology
Michal Solomon, Nadav Astman, Karin Warshavsky, Aviv Barzilai, Tal Meningher, Dror Avni, Eli Schwartz
Summary: This article reports 8 cases of Leishmania infantum cutaneous leishmaniasis in Israel, with 7 cases occurring during 2020-2021, suggesting that L. infantum may be an emerging infection in Israel.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Biology
A. C. F. H. Ramos-Milare, B. G. Sydor, A. A. Brustolin, D. S. S. L. Lera-Nonose, J. Oyama, E. L. Silva, W. Caetano, K. S. S. Campanholi, I. G. Demarchi, T. G. V. Silveira, M. V. C. Lonardoni
Summary: This study evaluated the activity of bioactive naphthoquinones against Leishmania amazonensis and found that they significantly inhibited the survival rate of the parasite and induced changes in cell function. These naphthoquinones may be promising candidates for research on new drugs with anti-Leishmania activity derived from natural products.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Edlainne Pinheiro Ferreira-Sena, Daiana de Jesus Hardoim, Flavia de Oliveira Cardoso, Luiz Ney d'Escoffier, Isabela Ferreira Soares, Joao Pedro Rangel da Silva Carvalho, Ricardo Almir Angnes, Stenio Perdigao Fragoso, Carlos Roberto Alves, Salvatore Giovanni De-Simone, Josue da Costa Lima-Junior, Alvaro Luiz Bertho, Tania Zaverucha-do-Valle, Franklin Souza da Silva, Katia da Silva Calabrese
Summary: Leishmaniasis is a complex and diverse disease that is considered a major public health problem. Despite the availability of treatment options, there are currently no vaccines for cutaneous leishmaniasis. This study focused on the prediction and characterization of antigenic epitopes that could interact with major histocompatibility complex class I in mice or humans. Through a combination of immunogenicity prediction and interaction assays, nine antigenic peptides were identified as strong candidates for the development of a peptide vaccine against leishmaniasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bruna Taciane da Silva Bortoleti, Mariana Barbosa Detoni, Manoela Daiele Goncalves, Fernanda Tomiotto-Pellissier, Taylon Felipe Silva, Virginia Marcia Concato, Ana Carolina Jacob Rodrigues, Amanda Cristina Carloto, Ricardo Luis Nascimento de Matos, Victor Fattori, Nilton Syogo Arakawa, Waldiceu Ap. Verri Jr, Idessania Nazareth Costa, Ivete Conchon-Costa, Milena Menegazzo Miranda-Sapla, Pryscilla Fanini Wowk, Wander Rogerio Pavanelli
Summary: This study investigates the mechanism of SOL treatment in L. amazonensis-infected mice. The results show that SOL treatment does not induce toxicity and can reduce lesion size and parasite load, promote antioxidant and oxidative balance, and enhance anti-inflammatory responses, leading to accelerated wound healing and tissue repair.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Dermatology
Raimunda Nonata Ribeiro Sampaio, Marina Freitas Ferreira, Sofia Sales Martins, Jorgeth de Oliveira Carneiro da Motta
Summary: Diffuse cutaneous leishmaniasis is a rare systemic disease caused by different species, presenting a challenge with poor therapeutic response. A case in Brazil was successfully treated with early intervention, resulting in clinical cure after 30 months of follow-up.
ANAIS BRASILEIROS DE DERMATOLOGIA
(2021)
Article
Pharmacology & Pharmacy
Sarah Kymberly Santos de Lima, italo Novaes Cavallone, Dolores Remedios Serrano, Brayan J. Anaya, Aikaterini Lalatsa, Marcia Dalastra Laurenti, Joao Henrique Ghilardi Lago, Dalete Christine da Silva Souza, Gabriela Pustiglione Marinsek, Beatriz Soares Lopes, Renata de Britto Mari, Luiz Felipe Domingues Passero, Bozena B. Michniak-Kohn
Summary: This study developed a topical formulation containing 8-HQ and demonstrated its efficacy in experimental cutaneous leishmaniasis. The formulation was able to reduce parasite burden and lesion size without causing significant side effects.
Article
Biotechnology & Applied Microbiology
Michal Witt, Miron Tokarski, Ewa Zietkiewicz, Arleta Lebioda, Maria Szczypek, Wojciech Falkowski, Przemyslaw Mrozowski, Teresa Kulak, Malgorzata Sobieszczanska, Magdalena Mrugalska-Banaszak, Tomasz Jurek, Tadeusz Dobosz
Summary: This study investigates the heart tissue remains of Thaddeus Kosciuszko as a possible cause of his disease and death. Using nanosequencing, DNA samples from wax surfaces, wooden plates, and linen cloth in contact with the object were analyzed. The results suggest that the presence of Propionibacterium acnes in the original heart tissue may have caused endocarditis and eventually led to rapid deterioration of health and death.
MICROBIAL CELL FACTORIES
(2022)
Article
Neurosciences
Gabriela da Paz Silveira, Mayari Eika Ishimura, Daniela Teixeira, Layla Tesla Galindo, Agnes Araujo Sardinha, Marimelia Porcionatto, Ieda Maria Longo-Maugeri
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Meeting Abstract
Cardiac & Cardiovascular Systems
Maria Cristina Izar, Waleria Fonzar, Francisco Fonseca, Henrique Fonseca, Tuany P. Silva, Alfredo Eyer, Daniela Teixeira, Maria E. R. Coste, Carolina Franca, Ieda Longo-Maugeri, Antonio Carvalho, Joao B. Pesquero
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2019)
Meeting Abstract
Cardiac & Cardiovascular Systems
W. Toledo Fonzar, F. Helfenstein Fonseca, H. A. Fonseca, C. N. Franca, C. Fonzar, T. Pereira, E. C. Rochael, D. Teixeira, I. L. Maugeri, A. Eyer, J. B. Pesquero, M. C. O. Izar
Article
Neurosciences
Laura N. Zamproni, Daniela Teixeira, Amanda A. Alliegro, Ieda L. Maugeri, Anne des Rieux, Marimelia A. Porcionatto
Article
Biochemistry & Molecular Biology
Simone Katz, Clara Lucia Barbieri, Fernanda Paula Martins Soler, Andreimar Martins Soares, Maria Cristina Chavantes, Stella Regina Zamuner
PROTEIN AND PEPTIDE LETTERS
(2020)
Article
Immunology
Danielle A. M. da Silva, Fabiana R. Santana, Simone Katz, Daniel M. Garcia, Daniela Teixeira, Ieda M. Longo-Maugeri, Clara L. Barbieri
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Maria E. R. Coste, Carolina N. Franca, Maria Cristina Izar, Daniela Teixeira, Mayari E. Ishimura, Ieda Longo-Maugeri, Amanda S. Bacchin, Henrique Tria Bianco, Flavio T. Moreira, Ibraim Masciarelli Pinto, Gilberto Szarf, Adriano Mendes Caixeta, Otavio Berwanger, Iran Goncalves Jr, Francisco A. H. Fonseca
ARQUIVOS BRASILEIROS DE CARDIOLOGIA
(2020)
Article
Immunology
Cristina M. Orikaza, Carina C. Pessoa, Fernanda Paladino, Pilar T. Florentino, Clara L. Barbieri, Hiro Goto, Eduardo Milton Ramos-Sanchez, Jose Franco Silveira, Michel Rabinovitch, Renato A. Mortara, Fernando Real
INFECTION AND IMMUNITY
(2020)
Article
Biochemistry & Molecular Biology
Ana C. A. Casarotti, Daniela Teixeira, Ieda M. Longo-Maugeri, Mayari E. Ishimura, Maria E. R. Coste, Henrique T. Bianco, Flavio T. Moreira, Amanda F. Bacchin, Maria C. Izar, Iran Goncalves, Adriano Caixeta, Gilberto Szarf, Ibraim M. Pinto, Francisco A. Fonseca
Summary: In patients with STEMI, despite early reperfusion, the amount of infarcted mass and ventricular performance are related to inflammatory responses triggered by circulating B lymphocytes. This study highlights the potential role of B lymphocytes in myocardial infarction outcomes.
BIOSCIENCE REPORTS
(2021)
Article
Parasitology
Fabiana Rodrigues de Santana, Danielle Aparecida Marino da Silva, Simone Katz, Cristina Mary Orikaza, Katia Cristina Oliveira, Clara Lucia Barbieri
Summary: This study evaluated the efficacy of treatment with a recombinant cysteine proteinase from Leishmania, rldccys1, combined with allopurinol or miltefosine in hamsters infected with Leishmania (Leishmania) infantum chagasi. The results showed a significant decrease in parasite load and lower levels of serum immunoglobulins in hamsters treated with the combination therapy. The treatment with miltefosine combined with rldccys1 also prevented relapse.
PARASITOLOGY RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Amanda S. Bacchin, Francisco A. H. Fonseca, Rui Povoa, Gilberto Szarf, Ibraim Masciarelli Pinto, Adriano Mendes Caixeta, Daniela Teixeira, Ieda Longo Maugeri, Mayari E. Ishimura, Maria E. R. Coste, Henrique Tria Bianco, Carolina N. Franca, Maria Cristina Izar
Summary: This study evaluated the effects of early pharmacoinvasive strategy on infarct size and left ventricular ejection fraction in elderly and non-elderly patients with ST segment elevation myocardial infarction (STEMI). The results showed that elderly patients had similar infarct size but better left ventricular function compared to non-elderly patients.
ARQUIVOS BRASILEIROS DE CARDIOLOGIA
(2023)
Article
Peripheral Vascular Disease
Waleria T. Fonzar, Francisco A. Fonseca, Henrique A. Fonseca, Tuany P. Silva, Alfredo A. Rodrigues, Daniela Teixeira, Mayari E. Ishimura, Maria E. Coste, Carolina N. Franca, Henrique T. Bianco, Magnus Gidlund, Rafael L. Morais, Clarissa A. Bittencourt, Carlos A. Fonzar, Viviane A. Sant'Anna, Ieda L. Maugeri, Joao B. Pesquero, Maria C. Izar
Summary: This study evaluated the modulatory role of the immune-inflammatory system in atherosclerosis burden in familial hypercholesterolemia (FH). The results showed that age and CD4+ T cells were associated with atherosclerosis burden in FH patients under high-intensity lipid-lowering therapy.
ATHEROSCLEROSIS PLUS
(2022)