Article
Public, Environmental & Occupational Health
Gemechu Chala, Tadesse Eguale, Fufa Abunna, Daniel Asrat, Andrew Stringer
Summary: A study in peri-urban Addis Ababa, Ethiopia, identified and characterized Campylobacter species in humans, animals, and water sources, with significant antimicrobial resistance observed. The prevalence of Campylobacter species was high, with most isolates resistant to multiple classes of antimicrobials, highlighting the need for a One Health approach to investigate and address zoonotic infectious diseases in livestock owning populations.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Public, Environmental & Occupational Health
Frederico Alves, Rita Castro, Miguel Pinto, Alexandra Nunes, Constanca Pomba, Manuela Oliveira, Leonor Silveira, Joao Paulo Gomes, Monica Oleastro
Summary: This study investigated the C. difficile strains in canine and feline populations and assessed their genetic overlap with human strains. The results showed that companion animals may be a source of infection for toxigenic and antimicrobial resistant C. difficile isolates associated with humans. This study provides important data on the genetic proximity between C. difficile isolates from animals and humans, contributing to future research on the role of animal reservoirs in the transmission networks and alerting to potential public health risks.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Manu Kumar Gundappa, Thu-Hien To, Lars Gronvold, Samuel A. M. Martin, Sigbjorn Lien, Juergen Geist, David Hazlerigg, Simen R. Sandve, Daniel J. Macqueen
Summary: This study investigates the long-term outcomes of autopolyploid rediploidization in salmonid fishes using genome-wide resolution. Analysis reveals an initial wave of rediploidization in the late Cretaceous followed by a period of genomic stasis and a second rediploidization wave in the early Eocene, coinciding with species diversification. Insights into potential functional outcomes of delayed rediploidization are provided through gene set enrichment, gene expression, and codon-based selection analyses. The study enhances our understanding of delayed autopolyploid rediploidization and has broad implications for future studies of WGD events.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Public, Environmental & Occupational Health
Folorunso O. Fasina, Olubunmi G. Fasanmi, Yilma J. Makonnen, Charles Bebay, Bernard Bett, Kristina Roesel
Summary: The study found that One Health networks and initiatives are widely distributed in Africa, but there is an imbalance in stakeholder representation; future efforts should focus on enhancing the promotion and implementation of One Health policies, promoting theoretical transformation, and establishing monitoring and evaluation frameworks.
Article
Chemistry, Medicinal
Carlos L. Correa-Martinez, Niklas C. J. Hagemeier, Neele J. Froboese, Stefanie Kampmeier
Summary: This study evaluated the risk of acquiring vancomycin-resistant enterococci (VRE) following treatment with vancomycin for C. difficile infections. The results suggest that both vancomycin and metronidazole increase the risk of VRE acquisition, with genetic comparison indicating that VRE acquisition is a result of both antibiotic selection and pathogen transmission.
Article
Biochemistry & Molecular Biology
Adriana Cabal, Gerhard Rab, Beatriz Daza-Prieto, Anna Stoeger, Nadine Peischl, Ali Chakeri, Solveig Solverod Mo, Harald Bock, Klemens Fuchs, Jasmin Sucher, Krista Rathammer, Petra Hasenberger, Silke Stadtbauer, Manuela Canica, Peter Strauss, Franz Allerberger, Markus Wogerbauer, Werner Ruppitsch
Summary: Antimicrobial resistance (AMR) is a public health issue caused by the misuse of antibiotics in both human and veterinary medicine. In a laboratory in Austria, various bacterial species were sampled from different compartments, and their antimicrobial resistance genes were detected. Early detection of antimicrobial resistance can help prevent its spread along the food/feed chain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Diego Lucas Neres Rodrigues, Francielly Morais-Rodrigues, Raquel Hurtado, Roselane Goncalves dos Santos, Daniela Camargos Costa, Debmalya Barh, Preetam Ghosh, Khalid J. Alzahrani, Siomar Castro Soares, Rommel Ramos, Aristoteles Goes-Neto, Vasco Azevedo, Flavia Figueira Aburjaile
Summary: The study investigates the resistome of Acinetobacter baumannii strains by phylogenetic, phylogenomic, and comparative genomics analyses, revealing high genomic plasticity, an open pan-genome, and species-specific resistance gene profiles throughout evolutionary history. This information can be used for developing new therapeutic methods to control this important bacterial pathogen.
Article
Biotechnology & Applied Microbiology
Su-Chen Lim, Deirdre A. Collins, Korakrit Imwattana, Daniel R. Knight, Sicilia Perumalsamy, Natasza M. R. Hain-Saunders, Papanin Putsathit, David Speers, Thomas Riley
Summary: The study found that Clostridium difficile infection is mainly imported from the community into hospitals rather than spreading within the healthcare system. These findings suggest that developing community-based infection prevention and control strategies could significantly lower rates of CDI in the hospital setting.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Biao Tang, Abubakar Siddique, Chenhao Jia, Abdelaziz Ed-Dra, Jing Wu, Hui Lin, Min Yue
Summary: Salmonella is a common cause of foodborne bacterial disease, with animal-borne foods being the primary source of transmission. However, there is limited knowledge about antimicrobial resistance and virulence of Salmonella based on a One Health approach in China. To address this, the genomic data of 134 Salmonella isolates from animal and meat samples in China were analyzed. The results showed that a majority of the isolates were multidrug-resistant and contained various virulence genes. This study highlights the use of whole-genome sequencing as a cost-effective method for comprehensive surveillance of foodborne Salmonella isolates in the One Health approach.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Review
Microbiology
Eleonora Cella, Marta Giovanetti, Francesca Benedetti, Fabio Scarpa, Catherine Johnston, Alessandra Borsetti, Giancarlo Ceccarelli, Taj Azarian, Davide Zella, Massimo Ciccozzi
Summary: Antibiotic resistance is a significant global health concern that requires a collaborative One Health approach. It poses a threat to human and animal health, as well as the effectiveness of medical treatments and veterinary interventions.
Article
Infectious Diseases
Arun Gonzales Decano, Kerry Pettigrew, Wilber Sabiiti, Derek J. Sloan, Stella Neema, Joel Bazira, John Kiiru, Hellen Onyango, Benon Asiimwe, Matthew T. G. Holden
Summary: This study investigates the burden of antimicrobial resistance (AMR) among uropathogens in East Africa, revealing a high prevalence of multi-drug resistant cases. The findings demonstrate the abundant acquired resistome and virulome repertoire in uropathogenic Escherichia coli and Klebsiella pneumoniae, mainly disseminated via clonal and horizontal transfer. Routine genomic surveillance is emphasized for high-resolution bacterial epidemiology of these important AMR pathogens.
Article
Genetics & Heredity
Jaime Brizuela, Rattagan Kajeekul, Thomas J. Roodsant, Athita Riwload, Parichart Boueroy, Auttapong Pattanapongpaibool, Janjira Thaipadungpanit, Piroon Jenjaroenpun, Thidathip Wongsurawat, Elizabeth M. Batty, Boas C. L. van der Putten, Constance Schultsz, Anusak Kerdsin
Summary: In March 2021, an outbreak of severe Streptococcus suis infections occurred in Nakhon Ratchasima province, Thailand, resulting in 19 confirmed cases and two deaths. The outbreak was traced back to a raw pork dish consumed by 241 people during a Buddhist ceremony. Whole genome sequencing revealed that the outbreak was caused by a novel sequence type strain of S. suis with antibiotic resistance.
MICROBIAL GENOMICS
(2023)
Article
Infectious Diseases
Julia Ade, Julia M. Riehm, Julia Stadler, Corinna Klose, Yury Zablotski, Mathias Ritzmann, Dolf Kuemmerlen
Summary: Antimicrobial resistance is a critical issue in One Health globally. A study in Bavaria, Germany analyzed antimicrobial resistance in 6569 Escherichia coli isolated from pigs from 2016 to 2020. The results showed a decrease in colistin resistance, an increase in enrofloxacin resistance, and constant ceftiofur resistance. Despite a decline in antimicrobial usage, only colistin resistance decreased, suggesting the need for additional measures to address antimicrobial resistance.
Article
Biochemical Research Methods
Hsuan-Lin Her, Po-Ting Lin, Yu-Wei Wu
Summary: This study introduced a method (PangenomeNet) to build co-functional networks from pan-genomes, demonstrating its effectiveness in inferring functions for hypothetical genes using Escherichia coli as an example. The constructed network revealed functional organization in antimicrobial resistomes, including drug-specific resistance genes and shared functional roles among different drug classes. The approach shows promise in providing holistic views on antimicrobial resistomes and formulating hypotheses for targeted experimental works.
BMC BIOINFORMATICS
(2021)
Article
Public, Environmental & Occupational Health
Nachiket Mor
Summary: Globally, zoonotic diseases present a significant public health challenge, especially in developing countries like India. Despite a general recognition of this reality, governments worldwide struggle to respond effectively. The prevailing belief is that achieving One Health requires cross-sectoral collaboration, but establishing the necessary prerequisites for such collaboration is difficult. This paper analyzes the organization of One Health in India, Bangladesh, Kenya, and Rwanda, highlighting the pathways of zoonotic spillovers and epidemics. Based on these findings, the paper concludes that building multiple focused units, despite potential duplication and unaddressed gaps, would have a greater chance of success.
Article
Microbiology
Deirdre A. Collins, Stephen Marcella, Michael Campbell, Thomas V. Riley
Summary: This study aimed to determine the incidence rates and epidemiology of Clostridium difficile infection (CDI) in Perth, Western Australia. The results showed a high incidence of CDI, with female patients experiencing a higher risk. The dominant molecular type of CDI was RT 014/020, but there was also high strain diversity, suggesting exposure to multiple reservoirs.
Article
Microbiology
Su-Chen Lim, Natasza M. R. Hain-Saunders, Korakrit Imwattana, Papanin Putsathit, Deirdre A. Collins, Thomas Riley
Summary: This study found a close relationship between environmental Clostridium difficile isolates and those from humans, possibly indicating an environmental transmission route. Toxigenic strains isolated from water sources were mainly of the 014/020 ribotype, with susceptibility to most antibiotics. High-resolution core-genome analysis revealed recent genetic relationships between human and water isolates.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Su-Chen Lim, Deirdre A. Collins, Korakrit Imwattana, Daniel R. Knight, Sicilia Perumalsamy, Natasza M. R. Hain-Saunders, Papanin Putsathit, David Speers, Thomas Riley
Summary: The study found that Clostridium difficile infection is mainly imported from the community into hospitals rather than spreading within the healthcare system. These findings suggest that developing community-based infection prevention and control strategies could significantly lower rates of CDI in the hospital setting.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Deirdre A. Collins, Thomas Riley
Summary: Clostridium difficile infection (CDI) is a urgent threat to patients worldwide, and recurrent CDI remains a high risk due to disruption of the gut microbiota. Traditional therapy with vancomycin and metronidazole carries a high risk of recurrence, leading to the development of novel narrow spectrum antimicrobial agents like fidaxomicin and ridinilazole. Ridinilazole shows promising results in vitro and in animal models, and phase I and II clinical trials have demonstrated its efficacy and superiority over vancomycin in reducing recurrence of CDI. Phase III trials are currently ongoing to further evaluate its potential in reducing recurrent CDI and its impact on healthcare systems.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Noel P. Pitcher, Jitendra R. Harjani, Yichao Zhao, Jianwen Jin, Daniel R. Knight, Lucy Li, Papanin Putsathit, Thomas Riley, Glen P. Carter, Jonathan B. Baell
Summary: Colonization of pathogenic bacteria in the GI tract is a significant risk factor for healthcare-associated infections. Efforts to develop effective decolonization agents have been largely unsuccessful. This study modified antimicrobial compounds to target bacterial pathogens in the GI tract and identified potential candidates for further development of colon-targeted antimicrobial agents.
Article
Microbiology
Peng An Khun, Long Duc Phi, Phuong Thi Pham, Ha Thi Thu Nguyen, Quyen Thi Huyen Vu, Deirdre A. Collins, Thomas V. Riley
Summary: This study investigated the prevalence, molecular epidemiology, and antimicrobial susceptibility of Clostridioides difficile in children with diarrhea in Vietnam. The results showed a high prevalence of C. difficile, but a comparatively low proportion of toxigenic strains.
Editorial Material
Microbiology
Maja Rupnik, Daniel Knight
Article
Genetics & Heredity
Korakrit Imwattana, Papanin Putsathit, Deirdre A. Collins, Teera Leepattarakit, Pattarachai Kiratisin, Thomas Riley, Daniel R. Knight
Summary: Clostridioides difficile PCR ribotype (RT) 017 is one of the most successful strains of C. difficile globally, and its global dissemination time and key factors have been determined through high-resolution genomic and Bayesian evolutionary analyses. Furthermore, the study found that C. difficile RT 017 may have been spreading between Asia and Europe since the Middle Ages and was introduced to North America in the 19th century.
MICROBIAL GENOMICS
(2022)
Article
Microbiology
Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Deirdre A. Collins, Thomas V. Riley
Summary: This study evaluated the epidemiology, molecular characteristics, and antimicrobial susceptibility of C. difficile isolated from adults with diarrhoea in Vietnam. The overall prevalence of C. difficile was 15.1%, with 9.8% being toxigenic strains and 6.3% being non-toxigenic strains. The prevalence of multidrug resistance was 27.3%. Rating: 8 out of 10.
Article
Microbiology
Korakrit Imwattana, Niraj Shivaperumal, Teera Leepattarakit, Pattarachai Kiratisin, Daniel R. Knight, Thomas V. Riley
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Microbiology
Nirajmohan Shivaperumal, Natasza M. R. Hain-Saunders, Barbara J. J. Chang, Thomas V. V. Riley, Daniel R. R. Knight
Summary: We report the isolation of two esculin hydrolysis-negative strains of C. difficile from soils in Western Australia, which produce white colonies on chromogenic media and belong to evolutionarily divergent clade C-III. This suggests that environmental C. difficile may contribute to C. difficile infection in community settings.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Karla Cautivo-Reyes, Daniel R. Knight, Deborah Bowie, Benjamin Moreira-Grez, Andrew S. Whiteley, Thomas V. Riley
Summary: The high prevalence and diversity of Clostridium difficile in Australian soils suggest that soils play a role in the survival and dissemination of this organism, potentially contributing to its transmission among native wildlife, production animals, and in community and hospital settings.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Natasza M. R. Hain-Saunders, Daniel R. Knight, Mieghan Bruce, David Byrne, Thomas V. Riley
Summary: Clostridioides difficile continues to be a problem in causing gastrointestinal disease in humans and animals. This study investigates the epidemiology of CDI in Australian horses and highlights the need for a One Health approach to surveillance.
Article
Biotechnology & Applied Microbiology
Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Nguyen Thi Bui, Quyen Thi Huyen Vu, Luong Duy Trinh, Deirdre A. Collins, Thomas Riley
Summary: This study investigated the prevalence, molecular type, and antimicrobial susceptibility of Clostridioides difficile in the environment in Vietnam. It found that the overall contamination rate of C. difficile was 24.5%, with the highest prevalence in pig farm and hospital soils. C. difficile was also found in pig feces and potato surfaces. These findings highlight the importance of environmental sources in the epidemiology of C. difficile infection in Vietnam.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)