Article
Horticulture
Tatjana Cvrkovic, Jelena Jovic, Oliver Krstic, Slavica Marinkovic, Miljana Jakovljevic, Milana Mitrovic, Ivo Tosevski
Summary: This study demonstrates the pivotal role of Dictyophara europaea as an intermediate vector in the transmission of 'Candidatus Phytoplasma solani' among different habitats and plants.
Article
Agronomy
Jelena Jovic, Ivo Tosevski
Summary: The study aims to develop molecular tools for discriminating three host plant associations of Hyalesthes obsoletus, a vector of 'Candidatus Phytoplasma solani'. Multiplex PCR was designed to differentiate the host plant associations based on the size of amplified fragments. The method provides valuable information on disease epidemiology and transmission routes when combined with 'Ca. P. solani' genotyping, offering insights into the phylogenetic lineage and natural host preferences of H. obsoletus.
Article
Biology
Elham Jamshidi, Sergio Murolo, Sareh Baghaee Ravari, Mohammad Salehi, Gianfranco Romanazzi
Summary: This study investigates the molecular variants of 'Candidatus Phytoplasma solani' in Iranian vineyards, discovering new variants and providing valuable information for understanding the epidemiological cycle(s) of Bois noir (BN) and managing the disease. The findings highlight the importance of genotyping 'Ca. P. solani' strains for accurately identifying and controlling the disease.
Article
Horticulture
V Naor, T. Zahavi, R. Barkai, N. Weiss, N. Mozes-Daube, O. Dror, C. Finkelstein, S. Ahron, O. Bahar, E. Zchori-Fein, L. Iasur-Kruh
Summary: A trial in a heavily infected vineyard in northern Israel for two consecutive years showed that the endophytic bacterium Frateuria defendens (Frd) acted as a biological control agent in reducing yellows symptoms in grapevines. The suspension of Frd cells applied by foliar spray penetrated the leaves during the growing period and improved yield by 10 to 20%. The results suggest the potential of Frd as an environmentally friendly means of controlling yellows diseases in grapevine.
Article
Plant Sciences
Sofia Casarin, Simone Vincenzi, Antonella Esposito, Luisa Filippin, Vally Forte, Elisa Angelini, Nadia Bertazzon
Summary: This study examined the mechanisms occurring within 'Tocai friulano' grapevines infected with Flavescence doree (FD), revealing diverse modulations of defence genes and accumulation of metabolites. Successful defence mechanisms allowed the compartmentalization of FD symptoms and phytoplasmas within the infected grapevines.
Article
Agriculture, Multidisciplinary
Fabio Quaglino, Alessandro Passera, Monica Faccincani, Abdelhameed Moussa, Alberto Pozzebon, Francesco Sanna, Paola Casati, Piero Attilio Bianco, Nicola Mori
Summary: The study identified ground-cover weeds associated with BN epidemiology, such as Convolvulus arvensis, Chenopodium album, Polygonum aviculare, and Trifolium repens. Molecular typing revealed that prevalent CaPsol strains in symptomatic grapevines were linked to specific weed species like bindweed and nettle. Further research is needed to explore the role of these weeds in hosting CaPsol and transmitting it to grapevines, as well as the relationship with alternative vectors.
ANNALS OF APPLIED BIOLOGY
(2021)
Article
Plant Sciences
Alessandra Zombardo, Stefano Meneghetti, Giacomo Morreale, Antonio Calo, Angelo Costacurta, Paolo Storchi
Summary: This study used molecular markers to investigate the biotypes and genetic variability of two common grape varieties in Italy. The results identified three different genotypes belonging to Sangiovese, Sanforte, and Montepulciano. The study demonstrated the importance of DNA marker-based methods for varietal identification and studying genetic variability in grapevines.
Article
Agronomy
Stefano Panno, Andrea Giovanni Caruso, Sofia Bertacca, Antonino Pisciotta, Rosario Di Lorenzo, Serafino Marchione, Slavica Matic, Salvatore Davino
Summary: The study in Sicily, Italy, examined the presence and spread of GFLV in commercial vineyards, showing a certain degree of variability among Sicilian isolates, likely due to the continuous interchange of GFLV-infected propagating material with other regions or countries.
Article
Plant Sciences
Elena Porotikova, Uliana Terehova, Vitalii Volodin, Eugeniya Yurchenko, Svetlana Vinogradova
Summary: A large-scale phytosanitary monitoring in Russian vineyards revealed that over half of the samples in the commercial vineyards in the Krasnodar region, Stavropol region, and Republic of Crimea were positive for multiple grapevine viruses. Phylogenetic analysis identified the presence of different groups of viruses, highlighting the urgent need to develop and implement long-term strategies for controlling viral diseases in grape cultivation.
Article
Multidisciplinary Sciences
Andrea Kosovac, Zivko Curcic, Jelena Stepanovic, Emil Rekanovic, Bojan Duduk
Summary: In the study of rubbery taproot disease (RTD) in a sugar beet field in Rimski Sancevi, Serbia, three scenarios of RTD occurrence were investigated using a molecular epidemiology approach. It was discovered that Hyalesthes obsoletus ex Convolvulus arvensis is a vector for the RTD-causing bacterium CaPsol, and two other cixiids, Reptalus quinquecostatus and R. cuspidatus, were identified as vectors for the disease in crops. Understanding CaPsol epidemiological pathways and insect vectors is crucial for predicting disease occurrence and severity.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Monica Blanco-Meneses, Mauricio Serrano-Porras, Anny Calderon-Abarca, Alejandro Sebiani-Calvo, Gabriel Vargas, Oscar Castro-Zuniga
Summary: This study aimed to determine the Foa races present in Costa Rica and evaluate the tolerance of imported commercial celery cultivars. The study found three haplotypes closely related to Foa race 3, which were highly virulent and affected all cultivars resistant to races 2 and 4. This highlights the need for celery producers in Costa Rica to access seeds resistant to Foa race 3.
Article
Agronomy
Karima Ben Mansour, Adrian J. Gibbs, Noemi Messmer, Rene Fuchs, Thierry Wetzel, Patrick Winterhagen
Summary: GPGV is believed to have originated from wild and cultivated Vitis species in Northeast Asia, particularly Vitis coignetiae, around 3500 years ago. It likely infected Eurasian grape when it was first introduced to China during the Han Dynasty. The virus then spread to Europe around 1800 CE and eventually worldwide. German isolates were found within the post-1800 CE phylogeny, suggesting the early stages of GPGV invasion in Europe occurred in Germany rather than Italy. These findings provide significant insights into the origins and spread of GPGV.
Article
Biology
Ivo Ercole Rigamonti, Martino Salvetti, Paola Girgenti, Piero Attilio Bianco, Fabio Quaglino
Summary: Flavescence doree (FD) is a significant phytoplasma-associated disease in Europe, and recent studies have shown a great genetic diversity within FD phytoplasma strains. The disease can be spread by various vectors and affects not only grapes but also other plants. This study focused on investigating FD epidemiology in north-western Italy and identified FDp genotypes in symptomatic grapevines and other plants, expanding the knowledge on the range of host plants involved in disease spread.
Article
Plant Sciences
Bhanu Priya Donda, Sandya R. Kesoju, Kari Arnold, Neil McRoberts, Rayapati A. Naidu
Summary: In this study, the spatial and temporal spread of grapevine leafroll disease (GLD) in three vineyard blocks in eastern Washington State was examined. The results showed a gradual increase in disease incidence over time in all three blocks. The mapping of GLD provided valuable insights for improved area-wide disease management strategies.
Review
Horticulture
Anca Butiuc-Keul, Ana Coste
Summary: Grapevine cultivation has adapted to changing conditions and demands through advancements in biotechnology and molecular biology, allowing for genetic improvement. These technologies have led to better cultivar identification, trait mapping, and genomic selection, as well as advances in grapevine regeneration, genetic transformation, and genome editing for enhanced yield and quality.
Article
Plant Sciences
Milana Mitrovic, Slavica Marinkovic, Tatjana Cvrkovic, Jelena Jovic, Oliver Krstic, Miljana Jakovljevic
Summary: Diseases associated with 'Candidatus Phytoplasma solani' ('Ca. P. solani') cause significant economic damage to crops. The transmission and persistence of these diseases in agroecosystems are determined by polyphagous insect vectors and wild plants as natural reservoirs. The stolbur phytoplasma, a pathogen native to Europe, is particularly influenced by fluctuating market demands and limited crop rotation in Serbia. To cope with disease outbreaks, an online Compendium was created, providing a risk assessment framework and decision-making options for land use and crop allocation.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Agronomy
Oliver Krstic, Tatjana Cvrkovic, Slavica Marinkovic, Miljana Jakovljevic, Milana Mitrovic, Ivo Tosevski, Jelena Jovic
Summary: This study provides data on the genetic diversity and ecological properties of Flavescence doree (FD) outbreaks in Serbia. The dominant genotype causing FD in Serbia is M51, with multiple outbreaks of M12 also recorded. Tree of heaven is reported as a new reservoir for FD in Serbian vineyards, along with clematis. The study also identifies new endemic genotypes and confirms the complexity of the FD ecological cycle.
Article
Agriculture, Multidisciplinary
Ivana Stankovic, Katarina Zecevic, Goran Delibasic, Jelena Jovic, Ivo Tosevski, Branka Krstic
Summary: This is the first report of GRSPaV presence in Serbia, highlighting the need for further monitoring and increased phytosanitary control measures to prevent its spreading and introduction of new virus variants.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2023)
Article
Agronomy
Jelena Jovic, Ivo Tosevski
Summary: The study aims to develop molecular tools for discriminating three host plant associations of Hyalesthes obsoletus, a vector of 'Candidatus Phytoplasma solani'. Multiplex PCR was designed to differentiate the host plant associations based on the size of amplified fragments. The method provides valuable information on disease epidemiology and transmission routes when combined with 'Ca. P. solani' genotyping, offering insights into the phylogenetic lineage and natural host preferences of H. obsoletus.
Article
Biotechnology & Applied Microbiology
Ivo Tosevski, Sharlene E. Sing, Roberto Caldara, David K. Weaver, Jelena Jovic, Oliver Krstic, Hariet L. Hinz
Summary: Our study reveals that the seed-feeding weevil originally identified as Gymnetron antirrhini in North America is actually a separate species, Rhinusa dieckmanni. This weevil species is more widely distributed than previously thought, utilizing multiple host plants in the Balkans and the Anatolian Plateau. The phenotype of the weevil is highly variable and dependent on the seed capsule size of the host population.
BIOLOGICAL CONTROL
(2023)
Article
Entomology
Biljana Vidovic, Tatjana Cvrkovic, Warea Orapa
Summary: This study describes and illustrates a new genus and species in Papua New Guinea, called Solenidiversum falcatariae gen. nov. sp. nov. It infests the plant Falcataria moluccana and causes erineum on both leaf surfaces. This new genus has distinctive characteristics such as solenidion on the inner side of tarsus I, fusion of tibiae of both legs with tarsus, presence of all leg and ventral opisthosomal setae, entire empodium, and scapular tubercles on the rear shield margin. The addition of this new genus and species increases the total number of Colopodacini tribe to 16 genera and 41 species. A key to distinguish the genera of Colopodacini tribe is provided.
Article
Plant Sciences
Sanja Radonjic, Oliver Krstic, Tatjana Cvrkovic, Snjezana Hrncic, Slavica Marinkovic, Milana Mitrovic, Ivo Tosevski, Jelena Jovic
Summary: Flavescence doree (FD) is a severe and epidemic grapevine yellows disease caused by FD phytoplasma (FDp), transmitted by Scaphoideus titanus leafhopper. Montenegro has been conducting preventive surveillance for FDp presence since 2018. In 2021, FDp was detected in a grapevine from Godinje, Crmnica wine subregion, and clematis plants and alder trees in various regions of Montenegro were found carrying related phytoplasmas. These findings emphasize the need for stringent management and monitoring of S. titanus in Montenegrin vineyards to prevent FDp epidemic outbreaks.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Horticulture
Tatjana Cvrkovic, Jelena Jovic, Oliver Krstic, Slavica Marinkovic, Miljana Jakovljevic, Milana Mitrovic, Ivo Tosevski
Summary: This study demonstrates the pivotal role of Dictyophara europaea as an intermediate vector in the transmission of 'Candidatus Phytoplasma solani' among different habitats and plants.