Article
Microbiology
Xuerui Bao, Mona Bove, Tom Coenye
Summary: The high tolerance of Pseudomonas aeruginosa biofilms in respiratory tract infections in cystic fibrosis contributes to the failure of antibiotic therapy. This study identified carbon sources that could enhance the inhibiting and/or eradicating activity of tobramycin, ciprofloxacin, and ceftazidime against P. aeruginosa PAO1 biofilms. The mechanisms underlying the enhanced biofilm eradicating activity were strain-dependent.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Giovanni Di Bonaventura, Veronica Lupetti, Simone De Fabritiis, Alessandra Piccirilli, Annamaria Porreca, Marta Di Nicola, Arianna Pompilio
Summary: This study evaluated the antibacterial and antivirulence activities of eight FDA-approved non-antibiotic drugs and their effects on biofilm formation. Ciclopirox and actinomycin D showed the best antibacterial activity and were most effective against preformed biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Giovanni Di Bonaventura, Veronica Lupetti, Fabio Verginelli, Sara Giancristofaro, Rosemary Barbieri, Giovanni Gherardi, Arianna Pompilio
Summary: This study evaluated the antibacterial, antibiofilm, and antivirulence activities of apramycin compared to tobramycin against P. aeruginosa in cystic fibrosis patients. Apramycin showed better efficacy than tobramycin, without cytotoxicity to bronchial epithelial CF cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marco Artini, Gianluca Vrenna, Marika Trecca, Vanessa Tuccio Guarna Assanti, Ersilia Vita Fiscarelli, Rosanna Papa, Laura Selan
Summary: Pseudomonas aeruginosa is a bacterium frequently involved in cystic fibrosis airway infections. It increases its virulence through biofilm, motility, toxin production, and invasion of host cells. The metalloprotease serratiopeptidase (SPEP) has been shown to impair the virulence-related properties of Gram-positive bacteria. In this study, the effects of SPEP on different physiological aspects related to the virulence of P. aeruginosa isolated from CF patients were investigated. The results showed that SPEP impaired attachment to inert surfaces and adhesion/invasion of eukaryotic cells. It also had strain-dependent effects on pyocyanin and pyoverdine production. Additionally, SPEP seemed to increase swarming motility and staphylolytic protease production. Further studies are needed to understand why different strains react differently to specific treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Thang Truong, Alexander Twist, Andrey Zaytsev, Emma C. L. Marrs, Audrey Perry, Graeme Turnbull, Sylvain Orenga, Stephen P. Stanforth, John D. Perry
Summary: The study evaluated the culture of P. aeruginosa from respiratory samples of CF patients using Pseudomonas aeruginosa chromogenic agar (PACA), which proved to be a highly effective culture medium for the isolation and specific detection of P. aeruginosa from respiratory samples.
Article
Immunology
Jiwar Al-Zawity, Faria Afzal, Aysha Awan, Daniela Nordhoff, Alexander Kleimann, Daniel Wesner, Tristan Montier, Tony Le Gall, Mareike Muller
Summary: This study investigates the influence of the steroid sex hormone estradiol (E-2) on biofilm formation of Pseudomonas aeruginosa in cystic fibrosis (CF) patients. The results show that E-2 treatment can increase the attached biofilm mass, remodel the biofilm ultrastructure, and potentially modulate the QS signaling in the biofilm.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Marco Artini, Rosanna Papa, Filippo Sapienza, Mijat Bozovic, Gianluca Vrenna, Vanessa Tuccio Guarna Assanti, Manuela Sabatino, Stefania Garzoli, Ersilia Vita Fiscarelli, Rino Ragno, Laura Selan
Summary: The study evaluated the biofilm growth modulation exerted by 61 commercial essential oils on a selected number of P. aeruginosa strains isolated from CF patients. Additionally, machine learning techniques were used to identify the essential oil chemical components responsible for the positive or negative modulation of bacterial biofilm formation.
Article
Microbiology
Mona Bove, Xuerui Bao, Andrea Sass, Aurelie Crabbe, Tom Coenye
Summary: The combination of QSI and tobramycin for eradicating Pseudomonas aeruginosa biofilms did not show evolution-proof characteristics, as bacterial resistance mutations were identified in genes involved in antibiotic resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Giovanna Batoni, Elisa Catelli, Esinguel Kaya, Arianna Pompilio, Marta Bianchi, Emilia Ghelardi, Giovanni Di Bonaventura, Semih Esin, Giuseppantonio Maisetta
Summary: Therapy of lung infections caused by Pseudomonas aeruginosa in cystic fibrosis patients is challenging due to the presence of sticky mucus and antibiotic-tolerant biofilm. This study evaluates the growth of probiotic strains in an artificial sputum medium and their effects on planktonic and biofilm growth of clinical strains of P. aeruginosa. The results show that Lacticaseibacillus rhamnosus and Lactiplantibacillus plantarum can grow in the sputum medium and inhibit the growth of P. aeruginosa, including the pre-formed biofilm.
Article
Microbiology
Giovanni Di Bonaventura, Veronica Lupetti, Andrea Di Giulio, Maurizio Muzzi, Alessandra Piccirilli, Lisa Cariani, Arianna Pompilio
Summary: Antibiotic resistance calls for new strategies in treating pulmonary infections in CF patients. Repurposing known drugs accelerates drug discovery and development. This study identified five potential hits from a library of 3,386 drugs, with antimicrobial activity against P. aeruginosa, biofilm inhibition, and low toxicity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Pharmacology & Pharmacy
Amin Rakhshan, Nazanin Farahbakhsh, Ghamartaj Khanbabaee, Seyed Ahmad Tabatabaii, Saeed Sadr, Maryam Hassanzad, Mohammad Sistanizad, Farzaneh Dastan, Mahmoud Hajipour, Amir Reza Bahadori, Bahador Mirrahimi
Summary: This study evaluated the concurrent use of inhaled and intravenous aminoglycoside antibiotics for treating pulmonary exacerbation caused by Pseudomonas aeruginosa in cystic fibrosis patients. The results showed that this treatment method was safe and effective, and it improved patients' forced expiratory volume and treatment outcomes.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Isaac Martin, Valerie Waters, Hartmut Grasemann
Summary: The treatment of lung infection in cystic fibrosis is limited by the biofilm growth of pathogenic organisms. Interventions targeting bacteria, extracellular matrix components, and signaling pathways offer potential new therapeutic strategies to overcome current limitations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Marco Artini, Esther Imperlini, Francesco Buonocore, Michela Relucenti, Fernando Porcelli, Orlando Donfrancesco, Vanessa Tuccio Guarna Assanti, Ersilia Vita Fiscarelli, Rosanna Papa, Laura Selan
Summary: This study demonstrates the anti-biofilm and anti-virulence activity of KHS-Cnd against clinical strains of Pseudomonas aeruginosa. It impairs biofilm development and causes biofilm disaggregation, while also reducing adhesion to host cells and inhibiting invasion. The potential of KHS-Cnd as a therapeutic agent against P. aeruginosa is highlighted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Esther Shmidov, Ilana Lebenthal-Loinger, Shira Roth, Sarit Karako-Lampert, Itzhak Zander, Sivan Shoshani, Amos Danielli, Ehud Banin
Summary: The functions of bacterial TA systems are still disputed and poorly understood. This study sheds light on potential functions of bacterial TA systems. The novel type II TA system PrrT/A in Pseudomonas aeruginosa is found to regulate biofilm formation, motility, prophage induction, bacteriophage production, and aminoglycoside resistance, providing new insights into the diverse roles and impacts of TA systems in bacteria.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Pierre-Henri Elchinger, Cedric Delattre, Sophie Faure, Olivier Roy, Stephanie Badel, Thierry Bernardi, Claude Taillefumier, Philippe Michaud
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2015)
Article
Microbiology
Elodie Olivares, Stephanie Badel-Berchoux, Christian Provot, Benoit Jaulhac, Gilles Prevost, Thierry Bernardi, Francois Jehl
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Veronique Tabone, Guillaume Pierre, Stephanie Badel-Berchoux, Christine Gardarin, Thierry Bernardi, Philippe Michaud
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2015)
Article
Biochemistry & Molecular Biology
P-H. Elchinger, C. Delattre, S. Faure, O. Roy, S. Badel, T. Bernardi, P. Michaud, C. Taillefumier
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2017)
Article
Immunology
Jason Tasse, Delphine Croisier, Stephanie Badel-Berchoux, Pascal Chavanet, Thierry Bernardi, Christian Provot, Frederic Laurent
PATHOGENS AND DISEASE
(2016)
Article
Multidisciplinary Sciences
Jason Tasse, Sophie Trouillet-Assant, Jerome Josse, Patricia Martins-Simoes, Florent Valour, Carole Langlois-Jacques, Stephanie Badel-Berchoux, Christian Provot, Thierry Bernardi, Tristan Ferry, Frederic Laurent
Article
Microbiology
Bo-Hyung Lee, Sophie Cole, Stephanie Badel-Berchoux, Laurent Guillier, Benjamin Felix, Nicolas Krezdorn, Michel Hebraud, Thierry Bernardi, Ibrahim Sultan, Pascal Piveteau
FRONTIERS IN MICROBIOLOGY
(2019)
Review
Microbiology
Elodie Olivares, Stephanie Badel-Berchoux, Christian Provot, Gilles Prevost, Thierry Bernardi, Francois Jehl
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Elodie Olivares, Jason Tasse, Stephanie Badel-Berchoux, Christian Provot, Gilles Prevost, Thierry Bernardi
Article
Infectious Diseases
Xavier Argemi, Gilles Prevost, Philippe Riegel, Christian Provot, Stephanie Badel-Berchoux, Francois Jehl, Elodie Olivares, Yves Hansmann
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2017)