Article
Chemistry, Multidisciplinary
Maria Teresa Rocchetti, Pasquale Russo, Giuseppe Spano, Letizia De Santis, Ilenia Iarusso, Nicola De Simone, Samira Brahimi, Daniela Fiocco, Vittorio Capozzi
Summary: This study reversed the traditional approach for probiotic screening by evaluating the in vitro probiotic properties of microbes already commercialized in the dairy sector. The analyzed strains showed good survival under stressful conditions, strong inhibitory activity against harmful bacteria, high adhesion rates, and anti-inflammatory effects.
APPLIED SCIENCES-BASEL
(2022)
Article
Immunology
Basavaprabhu Haranahalli Nataraj, Chette Ramesh, Rashmi Hogarehalli Mallappa
Summary: The study demonstrates that surface proteins derived from probiotics can effectively inhibit the adhesion of MRSA on mucosal and cellular surfaces, helping to prevent their intestinal infections.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Giovanna Batoni, Esingul Kaya, Elisa Catelli, Sabrina Quinti, Matteo Botti, Alessandro De Carli, Marta Bianchi, Giuseppantonio Maisetta, Semih Esin
Summary: The study aimed to evaluate the adhesion ability of commercial probiotic strains to human lung epithelial cells and their ability to prevent adhesion of the lung pathogen Pseudomonas aeruginosa and reduce inflammation. Lactobacillus acidophilus showed the highest adhesion ability and was effective in preventing adhesion of P. aeruginosa. L. acidophilus also reduced the release of IL-1β and IL-6 in PBMCs.
Article
Food Science & Technology
Thiwanya Choeisoongnern, Sasithorn Sirilun, Rungaroon Waditee-Sirisattha, Komsak Pintha, Sartjin Peerajan, Chaiyavat Chaiyasut
Summary: Probiotic Enterococcus faecium OV3-6 and its secreted active peptide have shown the ability to survive in simulated digestion conditions, reduce bacterial adhesion, inhibit growth of various bacteria, and produce stable antimicrobial peptides. These characteristics make it a potential probiotic and bio-preservative for applications in fermented foods and functional food or feed industries.
Article
Chemistry, Applied
S. Akbaba, D. Atila, D. Keskin, T. Tezcaner, A. Tezcaner
Summary: This study successfully developed a novel intraperitoneal patch with immunomodulatory property for treatment of hernia, regeneration of abdominal wall and prevention of intraperitoneal adhesions. The patch design includes polypropylene mesh, pullulan hydrogel, and fibroin/chitosan oligosaccharide lactate layer for immunomodulation and regeneration.
CARBOHYDRATE POLYMERS
(2021)
Article
Microbiology
P. R. Chandhni, Diwas Pradhan, Kandukuri Sowmya, Sunny Gupta, Saurabh Kadyan, Ritu Choudhary, Archita Gupta, Ganga Gulati, Rashmi Hogarehalli Mallappa, Jai K. K. Kaushik, Sunita Grover
Summary: The study demonstrated the therapeutic effects of probiotic surface proteins in colitis mice, offering a potential and safer alternative for the management of inflammatory bowel disorders.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sara Silva, Eduardo M. Costa, Helder Oliveira, Vitor De Freitas, Rui M. Morais, Conceicao Calhau, Manuela Pintado
Summary: Blueberry extracts have antimicrobial properties and can be used together with probiotics to reduce pathogen adhesion. The combination of blueberry extract with Bifidobacterium animalis can even completely inhibit pathogen adhesion.
Article
Microbiology
Fatima Alameri, Mohammad Tarique, Tareq Osaili, Riyad Obaid, Abdelmoneim Abdalla, Razan Masad, Ashraf Al-Sbiei, Maria Fernandez-Cabezudo, Shao-Quan Liu, Basel Al-Ramadi, Mutamed Ayyash
Summary: In this study, selected lactic acid bacteria (LAB) isolated from vegetable products were investigated for their potential probiotic characteristics, including resistance to acid and bile, cholesterol-reducing ability, production of exopolysaccharides (EPS), antimicrobial and hemolytic activities, and attachment capabilities. Some of the isolates also showed survival ability and attachment capabilities in the gastrointestinal tract.
Article
Multidisciplinary Sciences
Diletta Mazzantini, Marco Calvigioni, Francesco Celandroni, Antonella Lupetti, Emilia Ghelardi
Summary: The study found that different strains have different in vitro biological properties, highlighting the usefulness of in vitro tests as a prelude for clinical research.
SCIENTIFIC REPORTS
(2022)
Article
Nutrition & Dietetics
Patricia Diez-Echave, Izaskun Martin-Cabrejas, Jose Garrido-Mesa, Susana Langa, Teresa Vezza, Jose M. Landete, Laura Hidalgo-Garcia, Francesca Algieri, Melinda J. Mayer, Arjan Narbad, Ana Garcia-Lafuente, Margarita Medina, Alba Rodriguez-Nogales, Maria Elena Rodriguez-Cabezas, Julio Galvez, Juan L. Arques
Summary: Limosilactobacillus reuteri INIA P572 shows high resistance to gastrointestinal conditions, can grow and produce reuterin in a human colonic model, and exhibits clear immunomodulatory and protective effects in a DSS-induced colitic mice model.
Article
Microbiology
Ekaterina Khokhlova, Joan Colom, Annie Simon, Shahneela Mazhar, Guillermo Garcia-Lainez, Silvia Llopis, Nuria Gonzalez, Maria Enrique-Lopez, Beatriz Alvarez, Patricia Martorell, Marta Tortajada, John Deaton, Kieran Rea
Summary: Through clinical studies and in vitro experiments, we have identified the novel strain of B. clausii CSI08 (Munispore(R)) as a potential probiotic. This strain is resistant to gastric acid and bile salts, inhibits the growth of human pathogens, utilizes a wide range of carbohydrates, and produces beneficial enzymes. It also adheres to intestinal cells and reduces inflammation. Moreover, it exhibits cytoprotective effects in cell culture and animal models. Overall, these beneficial properties make B. clausii CSI08 a promising potential probiotic.
Article
Multidisciplinary Sciences
Bum Ju Kil, Young Jin Pyung, Hyunjoon Park, Jun-Won Kang, Cheol-Heui Yun, Chul Sung Huh
Summary: Recently, eukaryotic probiotics, particularly Saccharomyces cerevisiae yeast strains, have gained less attention compared to prokaryotic probiotics. In this study, novel yeast strains isolated from Korean fermented beverages were investigated for their potential probiotic characteristics. The selected strains showed various capabilities such as aggregation tendencies, co-aggregation with pathogens, hydrophobicity, antioxidant effects, survival in simulated gastrointestinal conditions, and adhesion to intestinal cells. Moreover, these strains had high cell wall glucan content and were confirmed as probiotics through genetic analysis. Additionally, S. cerevisiae strain GILA exhibited anti-inflammatory effects in both in vitro and in vivo experiments, suggesting its potential as a therapeutic probiotic for inflammation-related diseases.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Natalia C. Hernandez-Delgado, Edgar Torres-Maravilla, Lino Mayorga-Reyes, Rebeca Martin, Philippe Langella, Ricardo Perez-Pasten-Borja, Maria E. Sanchez-Pardo, Luis G. Bermudez-Humaran
Summary: This study isolated 20 potential probiotic strains from the agave fermentation stage in mezcal production, among which Lactobacillus rhamnosus LM07 and Lactobacillus plantarum LM17 and LM19 showed antioxidant and anti-inflammatory properties, indicating their potential therapeutic effects on intestinal inflammation.
Article
Biochemistry & Molecular Biology
Linan Tian, Tongtong Sun, Mengyao Fan, Hongyan Lu, Changshan Sun
Summary: In this paper, a novel hydrogel system composed of Antheraea pernyi silk protein (ASF) and tyramine-modified hyaluronic acid (HA-Ph) loaded with azithromycin (AZI), glucose oxidase (GOX), and horseradish peroxidase (HRP) was developed for the treatment of postoperative peritoneal adhesions. The hydrogel exhibited enhanced antibacterial ability due to GOX-catalyzed oxygen production and showed a retarding effect on AZI release. Furthermore, the hydrogel promoted the modulation of the TLR-4/Myd88/NF-kappa B pathway and TGF-beta/Smad2/3 pathway by azithromycin in the locally damaged peritoneum, resulting in accelerated tissue remodeling and reduced collagen deposition. Therefore, the application of this drug-loaded hydrogel system on postoperative abdominal wounds effectively inhibited the formation of postoperative adhesions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Immunology
Jose Maria Lemme-Dumit, Silvia Ines Cazorla, Gabriela Del Valle Perdigon, Carolina Maldonado-Galdeano
Summary: The study shows that oral administration of probiotics or their sub-cellular fraction can reduce bacterial burden in the spleen and liver after Salmonella challenge. The beneficial effects of probiotic-derived CW after challenge are attributed to Th1-type cell-mediated immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Luisa Martins Trindade, Licia Torres, Isabel David Matos, Vivian Correia Miranda, Luis Claudio Lima de Jesus, Gregorio Cavalcante, Jonathan Junio de Souza Oliveira, Geovanni Dantas Cassali, Pamela Mancha-Agresti, Vasco Ariston de Carvalho Azevedo, Tatiani Uceli Maioli, Valbert Nascimento Cardoso, Flaviano dos Santos Martins, Simone de Vasconcelos Generoso
Summary: Intestinal mucositis is a common side effect of cancer treatment, and the current management options are not very effective. Recent research suggests that the gut microbiota plays a role in the development and severity of mucositis. Therefore, the use of probiotics and paraprobiotics may have potential therapeutic effects on intestinal mucositis.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biotechnology & Applied Microbiology
Quesia S. Damaceno, Bruno Gallotti, Isabela M. M. Reis, Yasmim C. P. Totte, Gabriella B. Assis, Henrique C. Figueiredo, Tales F. Silva, Vasco Azevedo, Jacques R. Nicoli, Flaviano S. Martins
Summary: Breast milk contains beneficial bacteria that can positively influence the health of newborns. The study identified 77 bacteria belonging to four genera, including Staphylococcus, Streptococcus, Leuconostoc, and Lacticaseibacillus. Two potential probiotics, Lacticaseibacillus rhamnosus and Leuconostoc mesenteroides, were selected and found to colonize the gastrointestinal tract in germ-free mice and maintain viability after spray-drying.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biochemistry & Molecular Biology
Daniel Ferreira de Lima Neto, Vagner Fonseca, Ronaldo Jesus, Leonardo Hermes Dutra, Layssa Miranda de Oliveria Portela, Carla Freitas, Eduardo Fillizola, Breno Soares, Andre Luiz de Abreu, Sandeep Twiari, Vasco Azevedo, Aristoteles Goes-Neto, Arnaldo Correia de Medeiros, Norberto Peporine Lopes, Paolo Marinho de Andrade Zanotto, Rodrigo Bentes Kato
Summary: This study used the structure of SARS-CoV-2 spike protein to examine the impact of mutations on the protein's stability and interaction with ACE-2 receptor. Molecular dynamics simulations and protein-protein docking experiments revealed that the mutations affected the stability of the protein and improved its interaction with ACE-2 receptor, particularly in the Gamma variant.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Jessica C. das D. Ribeiro, Mariana M. Drumond, Pamela Mancha-Agresti, Joao P. F. Guimaraes, Daiane da C. Ferreira, Maria I. A. Martins, Pedro M. de M. Murata, Andressa C. de Carvalho, Raquel T. Pereira, Valdir Ribeiro Junior, Vasco A. de C. Azevedo, Luciana de P. Naves
Summary: This experiment evaluated the effects of probiotics on the performance and intestinal health of broilers exposed to heat stress. The results showed that diets containing non-commercial probiotics improved weight gain and feed conversion in broilers.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Review
Immunology
Mohamed Ahmed Raslan, Sara Ahmed Raslan, Eslam Mansour Shehata, Amr Saad Mahmoud, Nagwa A. Sabri, Khalid J. Alzahrani, Fuad M. Alzahrani, Saleh Alshammeri, Vasco Azevedo, Kenneth Lundstrom, Debmalya Barh
Summary: This systematic review investigated the value and success of COVID-19 vaccination in children. The majority of adverse events reported were mild to moderate, and most were unrelated to vaccination. Different types of vaccines provided excellent protection against COVID-19 in children. Further studies are needed to confirm the long-term safety and effectiveness of vaccinations.
Article
Microbiology
Danitza Xiomara Romero-Calle, Francisnei Pedrosa-Silva, Luiz Marcelo Ribeiro Tome, Thiago J. J. Sousa, Leila Thaise Santana de Oliveira Santos, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Raquel Guimaraes Benevides, Thiago M. M. Venancio, Craig Billington, Aristoteles Goes-Neto
Summary: In Brazil, Salmonella enterica serovar Enteritidis is a significant health threat. A strain of Salmonella enterica serovar Enteritidis SE3 was isolated from soil in Santo Amaro, Brazil, an area contaminated with heavy metals and organic waste. The genome analysis of this strain revealed the presence of important resistance and virulence genes, which are crucial for controlling Salmonella and heavy metal pollution in the region.
Article
Microbiology
Paula Cristina Silva Almeida, Bruno Stelmastchuk Roque, Andrei Giacchetto Felice, Arun Kumar Jaiswal, Sandeep Tiwari, Vasco Azevedo, Mario Leon Silva-Vergara, Siomar de Castro Soares, Kennio Ferreira-Paim, Fernanda Machado Fonseca
Summary: This study identified four potential vaccine candidates and drug targets through genome analysis, which could contribute to the development of vaccines and drugs that can change the current scenario of histoplasmosis treatment and prevention.
Article
Genetics & Heredity
Alessandra Lima, Ana Carolina Barbosa Caetano, Raquel Hurtado Castillo, Roselane Gonsalves dos Santos, Diego Lucas Neres Rodrigues, Thiago de Jesus Sousa, Rodrigo Bentes Kato, Marcus Vinicius Canario Viana, Anne Cybelle Pinto Gomide, Flavia Figueira Aburjaile, Sandeep Tiwari, Arun Jaiswal, Alfonso Gala-Garcia, Nubia Seyffert, Thiago Luiz de Paula Castro, Bertram Brenig, Mateus Matiuzzi da Costa, Elaine Maria Seles Dorneles, Yves Le Loir, Vasco Azevedo
Summary: This study performed comparative genomic analyses of 73 Staphylococcus aureus genomes from different hosts, including 12 genomes isolated from sheep. The study revealed the genomic specialization and the role of specific genes in establishing infection in ovine mastitis-associated S. aureus.
Article
Immunology
Debmalya Barh, Flavia Figueira Aburjaile, Thais Silva Tavares, Miguel Etcheverria da Silva, Gabriel Pissolati Mattos Bretz, Igor Fernando Martins Rocha, Annesha Dey, Renan Pedra de Souza, Aristoteles Goes-Neto, Servio Pontes Ribeiro, Khalid J. Alzahrani, Ahmad A. Alghamdi, Fuad M. Alzahrani, Ibrahim Faisal Halawani, Sandeep Tiwari, Alaa A. A. Aljabali, Kenneth Lundstrom, Vasco Azevedo, Nirmal Kumar Ganguly
Summary: In this study, a nutrigenomics approach was applied to investigate the association between dietary habits and COVID-19 severity and deaths. The results showed that distinct dietary habits of Indians, including increased consumption of certain food components and regular consumption of tea and turmeric, may contribute to the lower death rate from COVID-19 compared to western populations. These food components were found to suppress cytokine storm and various severity-related pathways of COVID-19.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Biochemical Research Methods
Carine R. Pereira, Raquel C. Neia, Saulo B. Silva, Charles H. D. Williamson, John D. Gillece, David O'Callaghan, Jeffrey T. Foster, Izabela R. C. Oliveira, Julio S. S. Bueno Filho, Andrey P. Lage, Vasco A. C. Azevedo, Elaine M. S. Dorneles
Summary: Numerous genotyping techniques are available for understanding the transmission dynamics of brucellosis, with varying costs and resolution levels. In this study, the genotypes of 53 Brazilian B. abortus isolates were compared using different genotyping methods, with MLVA showing higher resolution and cost-effectiveness, and SNP techniques and cgMLST showing the highest resolution and being recommended for outbreak investigations.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Microbiology
Monique Ferrary Americo, Andria dos Santos Freitas, Tales Fernando da Silva, Luis Claudio Lima de Jesus, Fernanda Alvarenga Lima Barroso, Gabriela Munis Campos, Rhayane Cristina Viegas Santos, Gabriel Camargos Gomes, Rafael Assis, Enio Ferreira, Pamela Mancha-Agresti, Juliana Guimaraes Laguna, Jean-Marc Chatel, Rodrigo Dias de Oliveira Carvalho, Vasco Azevedo
Summary: Mucositis is a gastrointestinal inflammation caused by chemotherapy treatments that severely affects patient's quality of life. Probiotic strains have shown promising results as an alternative treatment, and targeting the site of inflammation could be explored further. This study evaluated the anti-inflammatory effect of GDF11 delivered by Lactococcus lactis strains and found that the recombinant lactococci strains improved intestinal damage and reduced goblet cell degeneration in a murine model of mucositis induced by 5-FU. These strains also reduced neutrophil infiltration and exhibited immunomodulation of inflammatory markers, suggesting their potential as a gene therapy for 5-FU-induced intestinal mucositis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Sankaran Venkatachalam, Nisha Murlidharan, Sowmya R. Krishnan, C. Ramakrishnan, Mpho Setshedi, Ramesh Pandian, Debmalya Barh, Sandeep Tiwari, Vasco Azevedo, Yasien Sayed, M. Michael Gromiha
Summary: AIDS is a challenging infectious disease with a need for understanding drug resistance mechanisms. A new double-insertion mutation (L38HL) in HIV subtype C protease was investigated for its potential in inducing drug resistance towards the protease inhibitor Saquinavir (SQV). Computational techniques revealed that the L38HL mutation increased flexibility in certain regions and decreased binding affinity of SQV compared to wild-type. The mutation also resulted in a wide opening at the binding site and altered flap dynamics, leading to decreased interactions with the binding site and a potential drug resistance phenotype.
Article
Food Science & Technology
Marine Mantel, Tales Fernando da Silva, Rafael Gloria, Daniele Vassaux, Katia Duarte Vital, Valbert Nascimento Cardoso, Simone Odilia Antunes Fernandes, Eric Guedon, Yves Le Loir, Ana Maria Caetano Faria, Malvyne Rolli-Derkinderen, Vasco Azevedo, Gwenael Jan
Summary: Inflammatory bowel diseases (IBD) are a growing concern in western countries due to gut microbiota dysbiosis and loss of immunomodulatory bacteria. Probiotic products containing selected immunomodulatory bacterial strains, such as Propionibacterium freudenreichii, can mitigate IBD. The role of dairy matrix components in fermented dairy products and their effects on IBD remain unknown. This study found that P. freudenreichii-fermented milk, particularly with the presence of dairy fat, can prevent colitis and opens new perspectives for developing immunomodulatory functional fermented foods.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biochemistry & Molecular Biology
Anais Lavoisier, Martine Morzel, Severine Chevalier, Gwenaele Henry, Julien Jardin, Marielle Harel-Oger, Gilles Garric, Didier Dupont
Summary: This study investigated the digestion differences of high-protein dairy products in the elderly population. The results showed that the digestion rate in the gastric phase was slower in the older adult model, but no significant differences were observed in proteolysis in the intestinal phase compared to the young adult model.
Article
Cell & Tissue Engineering
Caroline C. C. Picoli, Patricia Rocha Martins, Xiao Lin Casey Wong, Thamires Righi, Pedro P. G. Guimaraes, Mauro C. X. Pinto, Jaime H. H. Amorim, Vasco A. C. Azevedo, Silma Regina Pereira, Alexandre Kanashiro, Fabio Cardoso Cruz, Rodrigo R. R. Resende, Akiva Mintz, Paul S. S. Frenette, Alexander Birbrair
Summary: Understanding the bone marrow niche is crucial for maintaining hematopoietic stem cells. This study presents a new method, called "whole bone subcutaneous transplantation", to precisely study the bone marrow niche in transgenic animals. By labeling cells, researchers were able to identify specific cellular components of the niche and found that certain cells play an important role in maintaining hematopoietic stem cells.
STEM CELL REVIEWS AND REPORTS
(2023)