Article
Fisheries
Cheng-dong Jin, Mo-fei Li
Summary: The study demonstrates that Omp1Et is a surface-located virulence factor of E. tarda, and recombinant Omp1Et can effectively induce immune protection as a subunit vaccine.
Article
Infectious Diseases
Yuying Fu, Lishan Zhang, Huanhuan Song, Junyan Liao, Li Lin, Wenjia Jiang, Xiaoyun Wu, Guibin Wang
Summary: This study investigates the role of lysine acetylation and succinylation in the antibiotic resistance mechanism of Edwardsiella tarda. The results demonstrate that these post-translational modifications play important roles in bacterial antibiotic resistance and are enriched in various metabolic pathways. The findings provide insights into the mechanism of antibiotic resistance and may aid future research on the pathogenesis of this bacterium.
Article
Biochemical Research Methods
Chao Wang, Xue-sa Dong, Yan-yan Yang, Guo-jing Xu, Meng-meng Wu, Fa-jun Yan, Long-gang Zhang, Li An, Pei-sheng Fu, Xi-rong Wang, Yu-bin Su, Qing-lei Meng
Summary: Metabolites in the TCA cycle increase the viability of bacteria against chloramphenicol, while NADPH promotes antibiotic resistance and oxidants restore the bactericidal ability. Additionally, the intracellular redox state determines the sensitivity to chloramphenicol, and the total antioxidative capacity is significantly reduced in antibiotic-resistant strains. These findings suggest that metabolite adjuvants should be cautiously applied, as they promote resistance to antibiotics.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Microbiology
Bei-bei Yan, Xue-sa Dong, Jun-peng Wang, Xiao-ying Li, Li An, Xi-rong Wang, Long-gang Zhang, Qing-lei Meng, Chao Wang
Summary: This study reveals that glutamate can promote chloramphenicol resistance by influencing glutathione biosynthesis and the tricarboxylic acid cycle. These findings are important for advancing our understanding of the impact of glutamate on antibiotic resistance.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Immunology
Shannon M. Collins, Angela C. Brown
Summary: Bacterial outer membrane vesicles (OMVs) play key roles in pathogenesis by delivering biomolecules to host cells, while also facilitating intra-bacterial communication processes, making them promising antibiotic delivery vehicles.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Kazuki Oishi, Moeri Morise, Linh Khanh Vo, Nhung Thi Tran, Daichi Sahashi, Rena Ueda-Wakamatsu, Wataru Nishimura, Masaharu Komatsu, Kazuhiro Shiozaki
Summary: The study suggests that the glycosphingolipid LacCer may act as a positive regulator in the invasion of E. tarda, by influencing the binding of bacteria to LacCer and GlcCer in microdomains.
CELLULAR MICROBIOLOGY
(2021)
Article
Fisheries
Mo-fei Li, Yu-ting Du, Cheng-dong Jin, Xue-peng Li, Yuan-yuan Sun
Summary: The study found that Ail1 protein in Edwardsiella piscicida plays a crucial role in its pathogenicity, affecting the survival and infection abilities of the bacteria as well as its virulence in hosts. This suggests that Ail1 may be explored as a potential subunit vaccine in aquaculture.
Article
Microbiology
Jingzhou Ye, Yubin Su, Xuanxian Peng, Hui Li
Summary: This study found that reactive oxygen species (ROS) play a role in ceftazidime resistance and sensitivity, with Fe3+ promoting ROS production by activating the pyruvate cycle. This discovery has important theoretical and practical implications.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
John William Young, Zhiyu Zhao, Irvinder Singh Wason, Franck Duong van Hoa
Summary: The outer membrane of Gram-negative bacteria is crucial for cell protection and antimicrobial discovery. A new method for isolating the outer membrane proteome has been developed, enabling further studies and potential discovery of antimicrobials.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Shao-hua Li, Jiao Xiang, Ying-yue Zeng, Xuan-xian Peng, Hui Li
Summary: This study investigates the tetracycline resistance mechanisms of Edwardsiella tarda using GC-MS based untargeted metabolomics and biochemistry and molecular biology techniques. The results indicate that elevated proton motive force (PMF) contributes to the resistance, and a combination of tetracycline with gentamicin can effectively kill resistant bacteria.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Laurent A. Bekale, Devesh Sharma, Brian Bacacao, Jing Chen, Peter L. Santa Maria
Summary: Bacteria develop tolerance after antibiotic exposure, followed by genetic resistance through the population of tolerant bacteria. Bacterial persister cells, a subpopulation within bacteria culture, maintain genetic susceptibility to antibiotics. The low metabolic activity of persister cells can be exploited for eradication, and gold nanoclusters coated with adenosine triphosphate (AuNC@ATP) can kill persister cells. Using AuNC@ATP, it is possible to prevent the emergence of antibiotic-resistant superbugs by eradicating persister cells in bacteria culture.
Article
Biology
Mofei Li, Meng Wu, Yuanyuan Sun, Li Sun
Summary: This study reveals the important role of E. tarda TraT in complement resistance and cellular invasion, providing insights into its strong evasion mechanisms.
COMMUNICATIONS BIOLOGY
(2022)
Article
Microbiology
Ming-Hsien Chiang, Fang-Ju Chang, Dinesh Kumar Kesavan, Aparna Vasudevan, Huaxi Xu, Kuo-Lun Lan, Shu-Wei Huang, Hung-Sheng Shang, Yi-Ping Chuang, Ya-Sung Yang, Te-Li Chen
Summary: This study reveals the immunological relevance and crucial role of imipenem-induced outer membrane vesicles (OMVs) from Elizabethkingia anophelis in bacterial survival under antibiotic stress conditions, rather than being a source of antibiotic resistance. Furthermore, this is the first study to discuss the protein-protein interaction network of OMVs released by E. anophelis, particularly under antibiotic stress. These findings provide important insights into clinical antibiotic stewardship.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Luis David Ginez, Aurora Osorio, Ricardo Vazquez-Ramirez, Thelma Arenas, Luis Mendoza, Laura Camarena, Sebastian Poggio
Summary: The outer membrane (OM) is crucial for Gram-negative bacterial cell envelope, with restricted diffusion of OM proteins and LPS creating a semi-crystalline state. The low fluidity of OM contributes to cell rigidity, with LPS characteristics strongly influencing OM properties and fluidity. Measurement of LPS fluidity can be challenging but important for understanding membrane dynamics.
Article
Veterinary Sciences
Harresh Adikesavalu, Thangapalam Jawahar Abraham, Siddhartha Narayan Joardar
Summary: This study assessed the effects of the outer membrane proteins (OMPs) of Edwardsiella tarda on immune response and antibody production in pangas catfish. The results showed that OMPs in combination with AH adjuvant can enhance immune response and antibody production, and provide protection against E. tarda infection in catfish.
VETERINARY RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Lu Li, Manjun Yang, Wei-cong Zhu, Xian-jie Liu, Xuan-xian Peng, Hui Li
Summary: Antibiotic resistance is a global public health concern, and understanding the mechanisms behind it is crucial for control. This study identified changes in the proteome of Escherichia coli in response to ampicillin, showing alterations in central carbon metabolism, oxidative energy production pathways, and fatty acid biosynthesis. Further investigation revealed specific proteins, such as AdhE, playing a role in resistance by regulating intracellular alcohol levels.
PROCESS BIOCHEMISTRY
(2021)
Article
Engineering, Environmental
Manjun Yang, Hui Liu, Bo Ye, Wei Qian
Summary: The study utilized abrasive waterjet (AWJ) cutting technology to crush waste PCBs, demonstrating its capability in effectively separating metals and nonmetals and its potential in environmentally friendly recovery of metal resources.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Biochemistry & Molecular Biology
Dai-xiao Yang, Hao Yang, Yun-chao Cao, Ming Jiang, Jun Zheng, Bo Peng
Summary: The study found that succinate is a crucial biomarker for phagocytosis in monocytes/macrophages, and exogenous succinate can enhance phagocytic rate, a process that is related to the TCA cycle. Furthermore, succinate also regulates gene expression associated with immune response and phagocytosis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Immunology
Man-jun Yang, Ming Jiang, Xuan-xian Peng, Hui Li
Summary: This study investigated how water temperature affects the survival of tilapia to bacterial infection from the perspective of metabolic state. It was found that tilapia were more susceptible to infection at 33 degrees C, but the infection could be attenuated by exogenous myo-inositol. The exogenous myo-inositol inactivated the elevated TCA cycle, increasing the survival ability of tilapia at higher water temperature.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Dai-Xiao Yang, Man-Jun Yang, Yue Yin, Tian-Shun Kou, Liao-Tian Peng, Zhuang-Gui Chen, Jun Zheng, Bo Peng
Summary: This study reveals the close association between overactive immune responses and metabolism, with exogenous serine identified as a potential therapeutic for regulating immune responses in fish upon bacterial infection. Such modulation of metabolism could be a promising strategy to control excessive immune responses.
Article
Biochemistry & Molecular Biology
Caixia Zhang, Lijun Xu, Jian Li, Jiansong Chen, Manjun Yang
Summary: This study sequenced and annotated the complete genome of H. coralloides, providing a valuable resource for studying the evolutionary diversification and bioactivity of mushrooms.
INTERNATIONAL JOURNAL OF GENOMICS
(2022)
Article
Biochemistry & Molecular Biology
Bo Ye, Jian Li, Lijun Xu, Hui Liu, Manjun Yang
Summary: This study reveals that supplementing Nile tilapia feed with black soldier fly larvae meal can improve fish growth rate, enhance energy metabolism and fatty acid synthesis, and improve the flavor of fish meat.
Article
Biochemistry & Molecular Biology
Jian-jun Tao, Juan-juan Xiang, Ming Jiang, Su-fang Kuang, Xuan-xian Peng, Hui Li
Summary: A modified microtitre plate dilution method, called the minimum killing concentration (MKC) test, was developed to evaluate the bactericidal efficacy of metabolites. The MKC test was suitable for different metabolites and clinically multidrug-resistant pathogens, and results correlated with the plate-counting test.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Microbiology
Ming Jiang, Zhuang-gui Chen, Hui Li, Tian-tuo Zhang, Man-jun Yang, Xuan-xian Peng, Bo Peng
Summary: Macrophages play a role in restricting bacterial infection by promoting phagocytosis and releasing cytokines and complement components. The expression of IL-1 beta and bacterial phagocytosis peak at 8 to 12 hours post-treatment, while the expression of complement component 3 continues to rise for 24 hours. Metabolomic analysis suggests a correlation between cellular concentrations of succinate and IL-1 beta, as well as inosine and C3. Inosine improves the survival of bacterial pathogen-infected mice and tilapia, indicating its potential therapeutic use in preventing and treating bacterial infections.
Article
Oncology
Xiao-li Yang, Peng Wang, Hua Ye, Ming Jiang, Yu-bin Su, Xuan-xian Peng, Hui Li, Jian-ying Zhang
Summary: Metabolomics is an efficient tool for screening biomarkers related to esophageal cancer. This study found that elevated amino acid metabolism and reduced carbohydrate metabolism are characteristic features of the disease. Metabolic differences were determined between different stages of the disease and between patients who received treatment and those who did not. Glycine, fructose, ornithine, and threonine were identified as potential biomarkers for evaluating disease prognosis and therapy in esophageal cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Infectious Diseases
Ming Jiang, Xia Li, Chun-Lin Xie, Peng Chen, Wei Luo, Chu-xiao Lin, Qiao Wang, Ding-Ming Shu, Cheng-long Luo, Hao Qu, Jian Ji
Summary: Salmonella enterica is a food-borne pathogen that poses a severe threat to both poultry production and human health. Understanding antibiotic resistance mechanisms and developing novel control measures are essential in combating antibiotic-resistant S. enterica.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Multidisciplinary Sciences
Ming Jiang, Yu -bin Su, Jin-zhou Ye, Hui Li, Su -fang Kuang, Jia-han Wu, Shao-hua Li, Xuan-xian Peng, Bo Peng
Summary: The research reveals that as ampicillin-sensitive strains acquire resistance to ampicillin, the abundance of glucose progressively decreases. Ampicillin triggers the event by targeting pts promoter and pyruvate dehydrogenase, leading to increased glucose transport and inhibited glycolysis. This process results in the activation of pentose phosphate pathway, generating reactive oxygen species and causing genetic mutations. The competitive binding of accumulated pyruvate and ampicillin gradually restores pyruvate dehydrogenase activity, lowers glucose level, and activates cAMP/CRP complex, inhibiting glucose transport and reactive oxygen species but enhancing DNA repair, ultimately leading to ampicillin resistance. Glucose and Mn2+ delay the acquisition of resistance, providing a promising approach to control antibiotic resistance.
Article
Biochemistry & Molecular Biology
Ming Jiang, Xin-Hai Chen, Hui Li, Xuan-Xian Peng, Bo Peng
Summary: Multidrug-resistant bacteria pose a significant threat to public health. Through proteomic and metabolomic analyses, the study identified a correlation between L-Alanine metabolism and phagocytosis with mouse survival in Vibrio parahaemolyticus infection. Experimental validation showed that exogenous L-Alanine promoted phagocytosis of multidrug-resistant pathogens, revealing the underlying mechanism.
Article
Oncology
Xun Chen, Chen Yi, Man-Jun Yang, Xueqi Sun, Xubin Liu, Hanyu Ma, Yiming Li, Hongyu Li, Chao Wang, Yi He, Guanhui Chen, Shangwu Chen, Li Yu, Dongsheng Yu
Summary: The study found distinct metabolic characteristics in oral precancerous cells and cervical precancerous lesions compared to normal and cancer cells. Enhanced metabolic activity and increased expression of glycolytic enzymes were observed in cervical precancerous lesions, suggesting metabolic reprogramming may contribute to early tumorigenesis.