Article
Infectious Diseases
Nicola Mangieri, Roberto Foschino, Claudia Picozzi
Summary: The study found that using bacteriophages can effectively reduce the number of E. coli bacteria producing biofilms, thus improving food safety. Preventing biofilm formation is more effective than trying to remove pre-formed biofilms.
Article
Immunology
Caroline Zellmer, Mohamad R. A. Sater, Miriam H. Huntley, Majdi Osman, Scott W. Olesen, Bharat Ramakrishna
Summary: Fecal microbiota transplantation is recommended for multiply recurrent Clostridioides difficile infection, but transmission of Shiga toxin-producing Escherichia coli from donors can lead to adverse events. Improved screening can help prevent future transmission.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Magali Fernandez, Maria Laura Casaux, Martin Fraga, Rafael Vignoli, Ines Bado, Pablo Zunino, Ana Umpierrez
Summary: The aim of this study was to characterize pathogenic Escherichia coli isolates from dead newborn calves in Uruguay. Various virulence genes were identified, and a high prevalence of STEC, EPEC, and NTEC infections was found. The potential role of EHEC (stx1/eae/ehxA) in calf mortality was indicated. Antimicrobial resistance and gene transmissibility pose a threat to public health.
Review
Immunology
Su-bin Hwang, Ramachandran Chelliah, Ji Eun Kang, Momna Rubab, Eric Banan-MwineDaliri, Fazle Elahi, Deog-Hwan Oh
Summary: STEC is a global foodborne bacterial pathogen that can cause severe diarrhea and other critical illnesses, mainly through inflammation caused by Shiga toxins. There is currently no widely accepted treatment for STEC infections, and further research is needed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
R. R. Atitkar, J. R. Hauser, A. R. Melton-Celsa
Summary: In a murine model of STEC infection, the stx(2a) phage lytic genes were found to be unnecessary for the virulence of pathogenic O157:H7 clinical isolates or for the release of Stx2a into bacterial cultures. These findings suggest an alternative mechanism for Stx2a release from STEC strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Scott T. Henderson, Pallavi Singh, David Knupp, David W. Lacher, Galeb S. Abu-Ali, James T. Rudrik, Shannon D. Manning
Summary: In this study, PCR assays were used to examine the occupancy of Shiga toxin prophages in different loci among 346 E. coli O157 strains, revealing variations in occupancy profiles among different clades. Strains belonging to clade 8 were found to have unique prophage occupancy profiles that could impact toxin expression, providing insights into the complexity of prophage integration and its implications on virulence.
Article
Food Science & Technology
Lin Walker, Shengqian Sun, Harshavardhan Thippareddi
Summary: The aim of this study was to compare the growth of non-O157 Shiga toxin-producing Escherichia coli (STEC) with E. coli O157:H7 in ground beef and modified Tryptic Soy Broth (TSB). Three available models (ComBase, Huang et al. model, and Cepeda et al. model) for predicting STEC growth in ground beef were evaluated. The study found that the growth of non-O157 STEC was similar to that of E. coli O157: H7, indicating that growth models for E. coli O157:H7 can be used to predict the growth of non-O157 STEC. Among the three models tested, the Cepeda et al. model accurately predicted STEC growth in ground beef and is considered a conservative growth model for STEC in commercial ground beef products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Roberto M. Vidal, David A. Montero, Felipe Del Canto, Juan C. Salazar, Carolina Arellano, Alhejandra Alvarez, Nora L. Padola, Hernan Moscuzza, Analia Etcheverria, Daniel Fernandez, Victoria Velez, Mauro Garcia, Rocio Colello, Marcelo Sanz, Angel Onate
Summary: In this study, the safety and immunogenicity of a vaccine candidate against Shiga toxin-producing Escherichia coli (STEC) was evaluated. The vaccine, formulated with two chimeric proteins, was administered to pregnant cows in their third trimester of gestation. The results demonstrated that the vaccine is safe and immunogenic, suggesting the feasibility of vaccinating cows with it during late pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Danielle M. Tack, Hannah M. Kisselburgh, LaTonia C. Richardson, Aimee Geissler, Patricia M. Griffin, Daniel C. Payne, Brigette L. Gleason
Summary: The study summarizes the epidemiology and transmission routes of STEC outbreaks in the United States from 2010 to 2017, finding that foodborne transmission is the most common route, and there are differences between O157 and non-O157 outbreaks in terms of food sources, demographic characteristics, and severity.
Article
Biotechnology & Applied Microbiology
Siobhan C. McCarthy, Guerrino Macori, Gina Duggan, Catherine M. Burgess, Seamus Fanning, Geraldine Duffy
Summary: This study found a high prevalence of STEC circulating within sheep in Ireland, with prevalence related to animal age and seasonality. Sheep harbor a variety of non-O157 STEC, whose prevalence and contribution to human disease have been underinvestigated for many years. A variety of Stx variants were also observed, some of which are of high clinical importance.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Michelle Qiu Carter, Nicole Laniohan, Chien-Chi Lo, Patrick S. G. Chain
Summary: This study examines the pathogenic potential of Shiga toxin-producing Escherichia coli (STEC) O145:H28 in clinical and environmental strains using comparative genomics. The core genes-based tree failed to differentiate between environmental and clinical strains, while the accessory genes-based tree grouped all clinical strains together. Loss-of-function mutations were common in virulence genes related to adherence and secretion systems. Differences in pathogenicity islands and other genetic elements were observed between O145:H28 and reference strains. The study reveals the genetic diversity and evolution of virulence in STEC.
Article
Food Science & Technology
Fangli Nong, Ping Zhang, Jing Meng, Qingchao Xie, Yufeng Li, Yingjie Pan, Yong Zhao, Haiquan Liu
Summary: This study found 49 strains of Shiga toxin-producing Escherichia coli (STEC) in retail raw meats in Southeast China, belonging to 33 divergent O:H serotypes. These STEC strains carried multiple virulence factors, showed resistance to various antibiotics, and had the ability to form biofilms, posing potential health risks to consumers.
Article
Immunology
Margherita Montalbano Di Filippo, Arianna Boni, Paola Chiani, Manuela Marra, Maria Carollo, Lucrezia Cristofari, Fabio Minelli, Arnold Knijn, Stefano Morabito
Summary: This study examines the interaction between STEC strains and Acanthamoeba sp., finding that STEC can survive within these amoebae and maintain their pathogenic potential. The internalization in free-living amoebae allows STEC to resist non-permissive growth conditions, escape detection, and withstand disinfection treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
David A. Montero, Richard Garcia-Betancourt, Roberto M. Vidal, Juliana Velasco, Pablo A. Palacios, Daniela Schneider, Carolina Vega, Leonardo Gomez, Hernan Montecinos, Rodrigo Soto-Shara, Angel Onate, Leandro J. Carreno
Summary: Researchers have developed a chimeric protein-based vaccine targeting seven virulence factors of Shiga toxin-producing Escherichia coli (STEC), which elicited significant immune responses in mice and provided protection against infection. However, further improvement is needed to enhance the vaccine's efficacy and explore other potential mechanisms of protection.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Microbiology
Ritesh Ray, Pallavi Singh
Summary: This article summarizes the research on the prevalence of STEC in livestock and wildlife hosts, and presents important microbial and environmental factors contributing to STEC spread and infections. The article also discusses potential strategies for limiting STEC shedding and transmission.
Article
Biochemical Research Methods
Teruyuki Miyake, Ryosei Tamaki, Moeko Asanuma, Yoji Fukada, Shun Hirota, Takashi Matsuo
BIOCONJUGATE CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Rini Majumder, Snigdha Roy, Kentaro Okamoto, Satoshi Nagao, Takashi Matsuo, Partha Pratim Parui
Article
Chemistry, Multidisciplinary
Catur Jatmika, Kazumo Wakabayashi, Ryosei Tamaki, Naoki Akiyama, Ibuki Nakamura, Shun Hirota, Hiroyasu Yamaguchi, Takashi Matsuo
Review
Chemistry, Physical
Takashi Matsuo
Summary: This review article discusses the application of designed Hoveyda-Grubbs-type complexes in bio-relevant studies, addressing issues and strategies when used in aqueous media, and controlling the reactivities of the complexes through structural modification. It also introduces a recent finding on the ruthenium complex transfer reaction between Hoveyda-Grubbs-type complexes and biomolecules.
Article
Biology
Makoto Mitsunaga, Kimihiro Ito, Takashi Nishimura, Hironori Miyata, Kei Miyakawa, Takeshi Morita, Akihide Ryo, Hisataka Kobayashi, Yoshimitsu Mizunoe, Tadayuki Iwase
Summary: The PIAS system is a targeted antimicrobial strategy that combines light-sensitive dye with monoclonal antibodies to eliminate fungal, viral, and bacterial pathogens, regardless of their drug resistance status. This strategy can act on a broad range of microbes, including drug-resistant strains, and has the potential to be a next-generation anti-infective therapy.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemical Research Methods
Tadayuki Iwase, Kimihiro Ito, Takashi Nishimura, Kei Miyakawa, Akihide Ryo, Hisataka Kobayashi, Makoto Mitsunaga
Summary: Microbial pathogens can develop resistance to drugs, so finding new therapeutic agents is crucial. This article introduces a photoimmuno-antimicrobial strategy (PIAS) that uses specific antibodies and dyes to target a wide range of microbes, including the SARS-CoV-2 virus and MRSA bacteria.
Article
Biochemistry & Molecular Biology
Ibuki Nakamura, Hiroshi Amesaka, Mizuho Hara, Kento Yonezawa, Keisuke Okamoto, Hironari Kamikubo, Shun-ichi Tanaka, Takashi Matsuo
Summary: This study investigates the applicability of synthetic binding proteins, specifically monobodies, in regulating the functions of proteins that undergo large conformational changes. The results show that monobodies can capture and stabilize specific conformational states of proteins, thereby affecting their functions. This suggests that monobody-based modulators can regulate the functions of proteins with significant conformational changes.
Article
Chemistry, Inorganic & Nuclear
Tsubasa Kinugawa, Takashi Matsuo
Summary: This paper investigates the effect of adding chalcogen atoms (sulfur or oxygen) to the benzylidene ligand of the Hoveyda-Grubbs second-generation complex (HG-II) on its catalytic properties. The study shows that the sulfur ligand enhances the stability and methanol tolerance of the catalyst, while the oxygen ligand decreases catalyst durability.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Yuji Mikata, Marin Tanaka, Saori Yasuda, Aya Tsuruta, Taeka Hagiwara, Hideo Konno, Takashi Matsuo
Summary: BQDMEN and its 6-methoxyquinoline derivative (6-MeOBQDMEN) are fluorescent sensors with minor response to Zn2+ and Cd2+. However, when three methoxy substituents are introduced at the 5,6,7-position of the quinoline rings, the fluorescent metal ion selectivity is reversed to favor Cd2+. This reversal of preference is also valid for 1,3-propanediamine derivatives. The dinuclear cadmium complex is suggested to be the key component for the fluorescent Cd2+ selectivity.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Yuji Mikata, Mizuho Uchida, Hinata Koike, Sunao Shoji, Yutaka Ohsedo, Yasushi Kawai, Takashi Matsuo
Summary: Eighteen mononuclear copper(II) complexes with oxygen-containing N4O1 pentadentate ligands were synthesized and the effects of substitution of (iso)quinoline heteroaromatics at different positions on the properties of copper(II) complexes were investigated. The results indicate that the steric hindrance caused by the peri hydrogen atom in quinoline at the Ar-3 position enhances the coordination ability of quinoline nitrogen and ether oxygen atoms to the metal center. The quinoline substitution at the Ar-3 site exhibits an opposite effect compared with those at the Ar-1 and Ar-2 sites in the absorption spectra.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Yuji Mikata, Minori Kaneda, Shizuka Yonemura, Miyu Akedo, Hideo Konno, Takashi Matsuo
Summary: The selectivity of TQEN towards metal ions was altered by introducing different substituents, with 8-MOMOTQEN showing high selectivity and sensitivity towards Cd2+. The MOMO group played a crucial role in enhancing fluorescent metal-ion selectivity.
DALTON TRANSACTIONS
(2022)
Letter
Medicine, Research & Experimental
Manami Tsunoi, Sunao Iyoda, Tadayuki Iwase
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Chemistry, Organic
Takashi Matsuo
Summary: Hoveyda-Grubbs-type complexes with ruthenium center coordinated by an N-heterocyclic carbene and a 2-alkoxybenzylidene ligands are important catalysts for olefin metathesis with applications in biochemical research. Structural modification allows for application of these complexes to biomolecules, while studies on the mechanism of olefin metathesis in aqueous media have been conducted.
JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN
(2021)
Article
Chemistry, Inorganic & Nuclear
Catur Jatmika, Kenta Goshima, Kazumo Wakabayashi, Naoki Akiyama, Shun Hirota, Takashi Matsuo
DALTON TRANSACTIONS
(2020)
Article
Infectious Diseases
Chiaki Okai, Yoshiro Itani, Akira Furuta, Yoshimitsu Mizunoe, Tadayuki Iwase
JAPANESE JOURNAL OF INFECTIOUS DISEASES
(2019)