4.1 Article

Ultrastructural observations of Beauveria bassiana infection in Xylotrechus rusticus larvae

Journal

ENTOMOLOGICAL RESEARCH
Volume 48, Issue 3, Pages 204-213

Publisher

WILEY
DOI: 10.1111/1748-5967.12273

Keywords

Beauveria bassiana; infection process; scanning electron microscopy; transmission electron microscopy; Xylotrechus rusticus

Categories

Funding

  1. National Scientific and Technological Project in the Twelfth Five-Year Plan of rural areas [2012BAD19B0704]

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In the present study we investigated the infection process of Beauveria bassiana on Xylotrechus rusticus larvae using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The SEM results showed that B. bassiana spores germinated on the surface of the larval body and invaded the larva as an appressorium. The hyphae then germinated from the spores and spread throughout the larval body. After the death of the larva, conidiophores formed at one end of the hypha on the surface of the larval body and prepared for a new round of infection. The TEM results showed severe damage to the larval cuticle after hyphae infection. The structure of the cuticle became thinner and eventually flocculent; muscle tissues were dissociated and eventually stuck to the hyphae, and the corpus adiposum was loose and deformed, and eventually degraded.

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