Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 5, Issue 9, Pages 7768-7776Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.7b01270
Keywords
Deep eutectic solvents; Choline chloride; Membrane protein; Reaction centers; Photosynthesis
Categories
Funding
- Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR), Research Project of National Interest [Prot. 2010C4R8M8]
- MIUR
- DiTECH (Molecular Nanotechnology for Health and Environment MAAT) [PON 02_00563_3316357]
- Interuniversities Consortium CINMPIS
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Deep eutectic solvents (DESs) are emerging as a new class of green solvents with the potential to replace organic solvents in several fundamental and applied processes. In this work, we offer an unprecedented characterization of the behavior of the bacterial photosynthetic reaction center (RC) from Rhodobacter sphaeroides in a series of choline chloride based DESs. RC is a membrane-spanning three-subunit pigment protein complex that, upon illumination, is capable of producing a stable charge-separated state. Thus, it represents the ideal model for carrying out basic studies of protein solvent interactions. Herein, we first report that, in many DES mixtures investigated, RC (a) is stable, (b) is capable of generating the charge-separated state, and (c) is even able to perform its natural photocycle. It proved, indeed, to be effective in reducing quinone molecules to quinol by withdrawing electrons from cytochrome c. As an example of biotechnological application, a photoelectrochemical cell based on DES-dissolved RC has also been designed and successfully employed to generate photocurrents arising from the reduction of the electron-donor ferrocenemethanol.
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