Article
Medicine, General & Internal
Saoussen Oueslati, Camille Gonzalez, Herve Volland, Vincent Cattoir, Sandrine Bernabeu, Delphine Girlich, Duncan Dulac, Marc Plaisance, Laure Boutigny, Laurent Dortet, Stephanie Simon, Thierry Naas
Summary: Vancomycin-resistant enterococci (VREs) are a major nosocomial pathogen worldwide, with rapid detection being crucial for prevention. The NG-Test VanB is an efficient and rapid assay with 100% sensitivity and specificity for confirming VanB-VREs. Its use can lead to more effective management of carriers and infected patients in less than 15 minutes.
Article
Multidisciplinary Sciences
Nader S. Abutaleb, Ahmed E. M. Elhassanny, Daniel P. Flaherty, Mohamed N. Seleem
Summary: Dorzolamide demonstrates potent activity against clinical VRE isolates and shows synergistic and additive interactions with gentamicin. In an in vivo mouse model, dorzolamide outperforms linezolid in reducing VRE colonization, suggesting it as a promising treatment option for VRE infections.
Article
Microbiology
Cecilia G. Carvalhaes, Helio S. Sader, Jennifer M. Streit, Mariana Castanheira, Rodrigo E. Mendes
Summary: According to data from a 10-year study, oritavancin has shown strong activity against Gram-positive pathogens that cause bloodstream infections, and has demonstrated good efficacy against resistant strains of MRSA and Enterococcus.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Medicine, General & Internal
He-Ning Wu, Er-Yan Yuan, Wen-Bin Li, Min Peng, Qing-Yu Zhang, Ke-liang Xie
Summary: This study retrospectively analyzed the microbiological and clinical data of bloodstream infection (BSI) patients in an ICU in Tianjin, China. The results showed that Gram-negative bacteria had the highest drug resistance rate and resulted in more severe brain damage, renal function damage, and thrombocytopenia. The test index of procalcitonin (PCT) could be used to distinguish Gram-negative bacteremia from Gram-positive and differentiate bacteremia from fungemia, but further research is needed.
FRONTIERS IN MEDICINE
(2022)
Article
Environmental Sciences
Nicoletta Makowska, Katarzyna Bresa, Ryszard Koczura, Anna Philips, Katarzyna Nowis, Joanna Mokracka
Summary: The study revealed the presence of dangerous antibiotic-resistant pathogens such as MRSA and VRE in urban sewage, with their frequency increasing during wastewater treatment. Furthermore, wastewater treatment was found to increase the relative abundance of mecA and virulence genes, indicating the existence of pathogenic bacteria in the effluent.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Hui Gao, Fuqiang Zhao, Ruijing Li, Shuaichen Jin, Haibo Zhang, Keyu Zhang, Shisheng Li, Qin Shu, Guangshui Na
Summary: This study investigated the occurrence and distribution of antibiotics and antibiotic resistance genes (ARGs) in surface water environment, focusing on the Liaohe River Basin in China. The results showed the presence of multiple antibiotics and ARGs in the water, indicating pollution and spread of antibiotic resistance in the aquatic ecosystem. The pollution level of antibiotics and ARGs was higher in the Daliao River system compared to the Liaohe River system. Multidrug and aminoglycoside were the dominant types of ARGs, and mobile genetic elements (MGEs) played an important role in the occurrence and evolution of ARGs in water.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Engineering, Environmental
Jing Li, Lijuan Li, Qin Li, Wen Fang, Yonghao Sun, Yu Lu, Jing Wang, Yanrong Zhu, Yao Zhang
Summary: The upstream basin of Hanjiang River is crucial for China's South-to-North Water Diversion Project. The water and soil quality in the river have significant impacts on the environment and biology. This study explored the correlation between heavy metals and metal resistance genes in the river. The results showed that heavy metals had a greater impact on antibiotic resistance genes than antibiotics, and there was a significant positive correlation between resistance genes.
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
(2023)
Article
Environmental Sciences
Lin Liu, Shan-Bin Guang, Yu Xin, Jie Li, Guo-Fu Lin, Li-Qin Zeng, Shao-Qin He, Yu-Ming Zheng, Guan-Yu Chen, Quan-Bao Zhao
Summary: This study aimed to understand the characteristics and influencing factors of antibiotic resistant genes (ARGs) in the subtropical drinking water river-reservoir system. The results showed that various environmental factors had significant impacts on the abundance and distribution of ARGs, and bacterial host and resistance mechanisms were also correlated with ARGs.
ENVIRONMENTAL POLLUTION
(2023)
Article
Public, Environmental & Occupational Health
Jens Thomsen, Najiba M. Abdulrazzak, Hussain AlRand, Godfred Antony Menezes, Carole A. Moubareck, Dean B. Everett, Abiola Senok, Andreas Podbielski
Summary: Enterococci are causing concern due to their increasing resistance to antibiotics in the Middle East and North Africa region, and there is a lack of AMR surveillance data for enterococci in this area.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Infectious Diseases
Pai-Wei Su, Emirlyn Cheng Yang, Sin-Hua Moi, Cheng-Hong Yang, Li-Yeh Chuang
Summary: The problem of antibiotic-resistant strains is a global public health issue. This study focused on the multi-drug resistant Acinetobacter baumannii and identified several drug resistance genes in these strains. The study also found that these genes influenced biofilm formation and antibiotic resistance patterns.
Review
Microbiology
Alexandra A. Guffey, Patrick J. Loll
Summary: VRE poses a serious threat to human health and new therapeutics are urgently needed. The VanRS two-component system, with its key molecules VanS and VanR, may provide a potential target for new treatment options.
Article
Environmental Sciences
Zhao Zhao, Chunchen Li, Liangying Jiang, Dayong Wu, Huijuan Shi, Guohua Xiao, Yueqiang Guan, Xianjiang Kang
Summary: Antibiotic resistance genes (ARGs) and human pathogenic bacteria in urban rivers can potentially affect human health through the food chain and diffusion. Factors such as nutrient levels, heavy metals, total petroleum hydrocarbons (TPHs), phytoplankton, and bacterial communities in water and sediment can influence the distribution of ARGs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Chemistry, Analytical
Nor Dyana Zakaria, Hairul Hisham Hamzah, Ibrahim Luqman Salih, Venugopal Balakrishnan, Khairunisak Abdul Razak
Summary: Vancomycin-resistant Enterococci (VRE) genes are bacteria strains that are resistant to the antibiotic vancomycin. They exhibit phenotypic and genotypic variations and pose significant issues for hospitalized patients. This review summarizes methods for detecting VRE strains and explores the potential use of electrochemical DNA biosensors for detecting VRE genes. However, no information was found on the development of electrochemical biosensors for VRE genes, only for vancomycin-susceptible bacteria.
Article
Environmental Sciences
Silvia Bonetta, Andrea Di Cesare, Cristina Pignata, Raffaella Sabatino, Manuela Macri, Gianluca Corno, Marco Panizzolo, Sara Bonetta, Elisabetta Carraro
Summary: This study examines the fate of antibiotic resistance in the urban water cycle in Italy, focusing on the prevalence of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in wastewater treatment plants (WWTPs) and drinking water treatment plants (DWTPs). Plate counting and quantitative real-time PCR (qPCR) were used to quantify ARB (resistant to tetracycline, ampicillin, and sulfonamides) and measure the abundance of selected ARGs. The results show higher concentrations of ARB and ARGs in WWTPs compared to DWTPs, indicating that WWTPs are hotspots for antibiotic resistance spread. Although significant reductions were observed after treatment, none of the detected ARB or ARGs were completely removed from drinking water. Therefore, it is important to integrate culture-dependent and culture-independent methods to investigate antibiotic resistance dynamics in aquatic ecosystems involved in the urban water cycle, and to monitor the presence of ARB and ARGs, especially in drinking water, as it represents a potential route of transmission to humans.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Pedro Teixeira, Marta Tacao, Isabel Henriques
Summary: This study investigated the distribution and temporal variation of antibiotic resistant bacteria and genes in a highly polluted river in Portugal. The results showed that water quality was poor, particularly near pig farms, and multiple antibiotic resistant genes were detected. The downstream locations of wastewater treatment plants had higher abundances of antibiotic resistant genes. Human and animal waste were identified as potential sources of these genes, and water quality parameters were significantly correlated with gene abundances.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Yoshihiro Suzuki, Reina Hashimoto, Hui Xie, Emi Nishimura, Masateru Nishiyama, Kei Nukazawa, Satoshi Ishii
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Ecology
Kei Nukazawa, Shinsuke Kajiwara, Tsuyoshi Saito, Yoshihiro Suzuki
ECOLOGICAL ENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Atsushi Jikumaru, Satoshi Ishii, Tomoko Fukudome, Yasuhiko Kawahara, Atsushi Iguchi, Yoshifumi Masago, Kei Nukazawa, Yoshihiro Suzuki
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2020)
Article
Multidisciplinary Sciences
Kei Nukazawa, Kentaro Akahoshi, Yoshihiro Suzuki
Article
Environmental Sciences
Kei Nukazawa, Kodai Shirasaka, Shinsuke Kajiwara, Tsuyoshi Saito, Mitsuteru Irie, Yoshihiro Suzuki
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Infectious Diseases
Emi Nishimura, Masateru Nishiyama, Kei Nukazawa, Yoshihiro Suzuki
Summary: This study compared the antibiotic resistance profile of Escherichia coli upstream and downstream of human habitation, finding no significant difference in resistance rates between different seasons and sampling sites. Additionally, significant differences were observed in the resistance profiles of E. coli between upstream and downstream sampling sites, indicating possible transmission or lack of antibiotic resistance genes as E. coli flows downstream.
Article
Environmental Sciences
Yoshihiro Suzuki, Mizuho Uno, Masateru Nishiyama, Kei Nukazawa, Yoshifumi Masago
Summary: The study proposed a new disinfection technology utilizing sunlight irradiation and seawater mixing to inactivate bacteria in wastewater, with the disinfection efficiency quantitatively evaluated for fecal indicator bacteria. Results showed a significant enhancement in disinfection effectiveness under specific salinity conditions, and a treatment system design was proposed based on detailed research.
JOURNAL OF WATER AND HEALTH
(2021)
Article
Infectious Diseases
Hui Xie, Yoshitoshi Ogura, Yoshihiro Suzuki
Summary: This study investigated the survival of E. coli in wastewater and the relationship between phylogenetic groups and antibiotic-resistant E. coli. The results showed that the prevalence of phylogroup B2 increased over time, along with an increase in antibiotic-resistant E. coli. The findings suggest that antibiotic-resistant B2 isolates have a higher survivability in treated wastewater.
Article
Public, Environmental & Occupational Health
Yoshihiro Suzuki, Hayate Hiroki, Hui Xie, Masateru Nishiyama, Shinsuke H. Sakamoto, Ryoko Uemura, Kei Nukazawa, Yoshitoshi Ogura, Toru Watanabe, Ikuo Kobayashi
Summary: In this study, antibiotic-resistant Escherichia coli strains isolated from a livestock farm were investigated, and it was found that when antimicrobials were managed and used cautiously, the resistance rate was low and there was no outbreak of resistance.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2022)
Article
Chemistry, Physical
Ryoya Yoshimura, SoeHtet Wai, Yasuyuki Ota, Kensuke Nishioka, Yoshihiro Suzuki
Summary: Hydrogen, a clean and renewable energy source, is a potential substitute for fossil fuels. In this study, the performance of a polymer electrolyte membrane (PEM) was investigated under different monovalent and divalent cation contaminations. The results showed that divalent cations have a stronger impact on the degradation of PEM compared to monovalent cations.
Article
Environmental Sciences
Yoshihiro Suzuki, Atsushi Jikumaru, Soichiro Tamai, Kei Nukazawa, Yoshifumi Masago, Satoshi Ishii
Summary: A major challenge in detecting waterborne pathogens is the low concentration of target bacteria in water. In this study, the coagulation and foam concentration method was used to obtain DNA from water samples, and 16S rRNA gene sequencing was conducted to analyze microbial community shifts and identify potentially pathogenic bacteria.
Article
Microbiology
Soichiro Tamai, Hiroshi Shimamoto, Kei Nukazawa, Yoshihiro Suzuki
Summary: This study finds that high counts of bacteria are present in beach sand, posing potential health threats to humans. The investigations reveal that coliform counts in the top surface sand increase significantly with rainfall, leading to changes in the composition of these bacteria. Some bacteria with suspected pathogenicity were found in the beach sand, emphasizing the importance of bacteria control for public health improvement.
Article
Engineering, Chemical
Tomohiro Yadai, Yoshihiro Suzuki
Summary: Efficient desalination requires the removal of blockade-causing substances from seawater. The combination of traditional softening and ballasted flocculation can effectively remove Ca2+ and Mg2+, as well as suspended solids and bacteria, improving the treatability of generated sludge.
Article
Engineering, Environmental
Yoshihiro Suzuki, Ryosuke Kaku, Katsuya Takahashi, Miyuka Kanai, Soichiro Tamai, Yuko Annaka, Nobuaki Chuganji
Summary: Ballasted flocculation achieves high-speed sedimentation of suspensions by using microsand and polymer flocculant combined with microflocs made of polyaluminum chloride (PAC) as an inorganic coagulant. This study investigated the effects of pH, PAC dosage, microsand and polymer dosages on treatment efficiency and floc settling velocity. The optimal conditions resulted in a floc settling velocity 17 times higher than conventional coagulation-sedimentation, with a turbidity of 0.54 TU and 99.7% removal efficiency. The study also provided fundamental information on dosage optimization and proposed removal mechanisms under different conditions.
WATER SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Environmental
Soichiro Tamai, Yoshitoshi Ogura, Miki Okuno, Kei Nukazawa, Yoshihiro Suzuki
Summary: In this study, a process was developed to concentrate exDNA adsorbed on colloids and dissolve them with an acid. The method successfully recovered uidA and tetA genes from genomic DNA and plasmids, respectively, and rapidly concentrated exDNA at a high concentration in wastewater effluent. Metagenomic analysis revealed the functions of some exDNA genes, including those encoding antibiotic resistance proteins. This study contributes to our understanding of horizontal gene transfer mechanisms in aquatic environments.
ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY
(2023)
