Article
Chemistry, Multidisciplinary
Bernardo Miller Naranjo, Semai Naicker, Oliver Lieleg
Summary: The study presents a testing setup for evaluating the functionality of coatings on endotracheal tubes. Different coating strategies were compared through ex vivo experiments, and it was found that covalent coatings generated from lab-purified mucins performed the best. This approach can also be applied to evaluate coatings on other medical tubing systems.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Immunology
Abigail E. Powell, Duo Xu, Gillie A. Roth, Kaiming Zhang, Wah Chiu, Eric A. Appel, Peter S. Kim
Summary: Ebola virus is a highly pathogenic virus that has caused numerous outbreaks. The existing vaccine requires ultra-low temperature storage, posing logistical challenges. By designing and characterizing two new vaccines, researchers found that some protein subunit antigens did not elicit enhanced antibody responses when displayed on multivalent scaffolds, providing insights for the design of future Ebola virus vaccines.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Hayley Gorman, France Moreau, Antoine Dufour, Kris Chadee
Summary: The colonic mucus bilayer plays a crucial role in the innate defense system, providing a habitat for the commensal microbiota. The study investigates the synthesis and interaction of FCGBP and MUC2 mucin in the mucus layer, and their impact on the structural integrity and epithelial barrier function.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yudi Liu, Jinyu Yi, Haokang Jia, Yutong Miao, Maolin Hou
Summary: The white-backed planthopper is one of the most important pests of rice in Asia, and the highly expressed SFMLP in its salivary glands plays a crucial role in its feeding. The study found that silencing SFMLP hindered the feeding ability of the planthopper and affected rice defense mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Kenrie P. Y. Hui, Ka -Chun Ng, John C. W. Ho, Hin-Wo Yeung, Rachel H. H. Ching, Haogao Gu, Joseph C. K. Chung, Velda L. Y. Chow, Ko-Yung Sit, Michael K. Y. Hsin, Timmy W. K. Au, Leo L. M. Poon, Malik Peiris, John M. Nicholls, Michael C. W. Chan
Summary: This study characterizes the replication competence and respiratory tissue tropism of different Omicron variants and finds that BA.2 has higher replication efficiency in nasal and bronchial tissues compared to wild-type, Delta and BA.1. The tested Omicron variants also show lower replication levels in lung tissues, which aligns with the milder clinical manifestations observed in patients infected with Omicron strains.
Article
Multidisciplinary Sciences
Lukasz Kurach, Agnieszka Chlopas-Konowalek, Barbara Budzynska, Marcin Zawadzki, Pawel Szpot, Anna Boguszewska-Czubara
Summary: Synthetic opioid etazene exhibits severe dose-dependent developmental toxicity and cardiotoxic effects in Danio rerio, with its metabolite potentially serving as a target for toxicological screening. Further research is needed to explore the exact mechanism of action of etazene.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Mun-Ock Kim, Jae-Won Lee, Jae Kyoung Lee, Yu Na Song, Eun Sol Oh, Hyunju Ro, Dahye Yoon, Yun-Hwa Jeong, Ji-Yoon Park, Sung-Tae Hong, Hyung Won Ryu, Su Ui Lee, Dae Young Lee
Summary: Korean black ginseng extract inhibits airway inflammation and mucus secretion by decreasing the activation of TAK1, suggesting it could be a potential adjuvant in the treatment of airway inflammatory diseases caused by cigarette smoke.
Article
Multidisciplinary Sciences
Kathrin Koedderitzsch, Ekaterina Zezina, Lingzi Li, Matthias Herrmann, Nadine Biesemann
Summary: The study revealed a direct role of TNF in the glycolytic reprogramming of FLS, confirming the potency of immunometabolism in RA. TNF stimulation was shown to enhance glycolysis, reduce GLUT1 and GAPDH levels, which may be linked to treatment response in patients.
SCIENTIFIC REPORTS
(2021)
Article
Endocrinology & Metabolism
Francesco Andreozzi, Concetta Di Fatta, Rosangela Spiga, Gaia Chiara Mannino, Elettra Mancuso, Carolina Averta, Carmen De Caro, Martina Tallarico, Antonio Leo, Rita Citraro, Emilio Russo, Giovambattista De Sarro, Giorgio Sesti
Summary: The study found a positive relationship between fasting glucagon levels and various proinflammatory cytokines and acute-phase reactants. Through animal experiments and in vitro cell experiments, the researchers discovered that glucagon can stimulate the activation of proinflammatory pathways in the liver, which may be related to hyperglycemia and cardiometabolic risk.
DIABETES OBESITY & METABOLISM
(2023)
Article
Cell Biology
Francesca Molinari, Alessandra Feraco, Simone Mirabilii, Serena Saladini, Luigi Sansone, Enza Vernucci, Giada Tomaselli, Vincenzo Marzolla, Dante Rotili, Matteo A. Russo, Maria Rosaria Ricciardi, Agostino Tafuri, Antonello Mai, Massimiliano Caprio, Marco Tafani, Andrea Armani
Summary: The inhibition of SIRT5 stimulates brown adipogenesis in vitro, promoting the expression of brown adipocyte and mitochondrial markers and increasing lipolysis. This approach shows potential as a strategy to target obesity and type 2 diabetes by activating brown adipose tissue.
Article
Biochemistry & Molecular Biology
Andrew Borgert, B. Lachele Foley, David Live
Summary: The study found that the conformational impact of α-O-Man and α-O-GaINAc modifications differs in the mucin-like region of ot-dystroglycan, with the former having limited effects on the peptide scaffold and the latter showing more profound effects. Structural analysis revealed a more extensive network of hydrogen bond interactions within the alpha-O-GaINAc-threonine residue, influencing the properties of the protein backbone. These conformational effects are relevant to the mechanical properties of alpha-dystroglycan.
Article
Multidisciplinary Sciences
Mary M. Walker, Molly E. Baumann, John H. Alexander, Britani N. Blackstone, Christopher B. Morgan, Thomas J. Scharschmidt, Heather M. Powell
Summary: Segmental bone defects present clinical challenges and mechanical expansion of periosteum can facilitate graft incorporation. Further research is needed to optimize the osteogenic potential.
Article
Chemistry, Medicinal
Wenbing Zhi, Shengnan Jiang, Zongren Xu, Yuye An, Jing Chen, Ye Li, Yang Liu, Hong Zhang
Summary: This study found that Oxysophocarpine (OSC) can alleviate asthma by reducing inflammation and mucus secretion, regulating immune responses, and suppressing the JNK/AP-1 signaling pathway.
Article
Biochemistry & Molecular Biology
Valentina Scalise, Chiara Sanguinetti, Tommaso Neri, Silvana Cianchetti, Michele Lai, Vittoria Carnicelli, Alessandro Celi, Roberto Pedrinelli
Summary: PCSK9 induces TF expression through activation of the TLR4/NFkB signaling pathway, with TF playing an essential role in both coagulation and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Natasha Haskey, Jiayu Ye, Mehrbod Estaki, Andrea A. Verdugo Meza, Jacqueline A. Barnett, Mitra Yousefi, Blake W. Birnie, Samantha Gruenheid, Sanjoy Ghosh, Deanna L. Gibson
Summary: There is a growing awareness of the interaction between diet, gut microbiota, and the immune system in the development of inflammatory bowel disease (IBD). High-fat diets have been shown to exacerbate IBD, while the fat blend found in the Mediterranean diet (MD) may have protective effects. A study in mice found that a diet mimicking the MD fat blend reduced disease activity and inflammation, and improved metabolic parameters in a mouse model of IBD.
Article
Biochemistry & Molecular Biology
Kuan Feng, Fei Deng, Zhihong Hu, Hualin Wang, Yun-Jia Ning
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Virology
Jun Wang, Minglu Yang, Haibing Xiao, Guo-Hua Huang, Fei Deng, Zhihong Hu
Letter
Cell Biology
Manli Wang, Ruiyuan Cao, Leike Zhang, Xinglou Yang, Jia Liu, Mingyue Xu, Zhengli Shi, Zhihong Hu, Wu Zhong, Gengfu Xiao
Letter
Cell Biology
Jia Liu, Ruiyuan Cao, Mingyue Xu, Xi Wang, Huanyu Zhang, Hengrui Hu, Yufeng Li, Zhihong Hu, Wu Zhong, Manli Wang
Article
Biotechnology & Applied Microbiology
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Summary: This study systematically analyzed the nuclear localization of 154 ORFs encoded by AcMNPV, with 25 viral proteins found to be localized in the nucleus in the absence of virus infection. Upon virus infection, 11 proteins originally located in the cytoplasm were completely translocated into the nucleus, suggesting their nuclear import is facilitated by other viral or host proteins. Further experiments identified specific proteins that were imported into the nucleus with the assistance of other nuclear localization proteins.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
Tong Chen, Xiaoyan Duan, Hengrui Hu, Yu Shang, Yangbo Hu, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu
Summary: The study investigated the impact of 42 genes of AcMNPV on the production of infectious BVs, revealing that 36 genes are dispensable, 3 genes are essential, and 3 genes are important but not essential for infectious BV production. The results contribute to understanding the functional genomics of baculoviruses and provide valuable information for future engineering of baculovirus expression systems.
Article
Virology
Mingyue Xu, Bo Wang, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu, Jia Liu
Summary: The establishment of a reverse genetics system based on a SFTSV C4 strain has revealed insights into virus replication and protein expression, and provides a valuable tool for the development of vaccines and antivirals against SFTS.
Correction
Virology
Mingyue Xu, Bo Wang, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu, Jia Liu
Article
Biochemical Research Methods
Hengrui Hu, Kai Pan, Yu Shang, Yijia Guo, Han Xiao, Fei Deng, Manli Wang, Zhihong Hu
Summary: The study utilized synthetic biology to investigate the fundamental function of baculoviral hrs through multiloci manipulation, revealing the importance of retaining a single hr for efficient viral DNA replication and progeny production. The research also identified the role of hrs in enhancing gene transcription within surrounding regions. Overall, the advantages of synthetic biology in engineering and functional studies of large DNA viruses were demonstrated.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Virology
Yu Shang, Hengrui Hu, Xi Wang, Hualin Wang, Fei Deng, Manli Wang, Zhihong Hu
Summary: Researchers successfully modified a synthetic baculovirus, AcMNPV-WIV-Syn1, into a novel bacmid, AcBac-Syn, for use as a backbone for baculovirus system. By constructing a vector containing a LacZ:attTn7 and egfp cassette and recombining it with the AcMNPV-WIV-Syn1 genome in yeast, they were able to generate the bacmid AcBac-Syn. The resulting bacmid showed similar biological characteristics to the wild-type virus and could be easily used for foreign gene insertion and expression.
Article
Virology
Yujia Liu, Xiaoqian Zhang, Dongbin Chen, Dehong Yang, Chenxu Zhu, Linmeng Tang, Xu Yang, Yaohui Wang, Xingyu Luo, Manli Wang, Yongping Huang, Zhihong Hu, Zulian Liu
Summary: This study demonstrates the successful prevention of BmNPV infection by disrupting the lef8 and lef9 genes using the CRISPR/Cas9 system. The replication and mortality of BmNPV were significantly reduced in the transgenic silkworms. These findings provide alternative targets for the CRISPR antiviral system and offer new ideas for virus infection research and insect pest control.
Article
Virology
Huanyu Zhang, Wenhua Kuang, Congcong Fu, Jiang Li, Manli Wang, Zhihong Hu
Summary: The study identified that the baculovirus core gene ac81 likely encodes a disulfide isomerase which plays a crucial role in various functions of the baculovirus life cycle. This suggests that viral disulfide bond formation could be an ancient mechanism shared by insect-specific large DNA viruses like baculoviruses, nudiviruses, and hytrosaviruses encoding AC81 and P33 (sulfhydryl oxidase).
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Yijia Guo, Hengrui Hu, Han Xiao, Fei Deng, Jiang Li, Manli Wang, Zhihong Hu
Summary: In this study, we used synthetic biology to optimize baculovirus expression vectors by reducing the number of genes through gene deletion. We demonstrated that the newly constructed virus is capable of producing infectious progeny viruses.
Article
Virology
Xiao Wang, Mingyue Xu, Huanhuan Ke, Longda Ma, Liushuai Li, Jiang Li, Fei Deng, Manli Wang, Zhihong Hu, Jia Liu
Summary: This study constructed a reporter virus for SFTSV, which can be used for drug screening in vitro. The results showed that this reporter virus can effectively screen antiviral drugs. Additionally, the reporter virus exhibited attenuated pathogenicity in mice, making it a promising tool for high-throughput screening of antiviral drugs in the future.
Letter
Cell Biology
Jia Liu, Yufeng Li, Liang Liu, Xudong Hu, Xi Wang, Hengrui Hu, Zhihong Hu, Yiwu Zhou, Manli Wang
