Article
Parasitology
Martin K. Nielsen, Chelsea Facison, Jessica A. Scare, Avery N. Martin, Holli S. Gravatte, Ashley E. Steuer
Summary: The study showed that diagnosing Strongylus vulgaris on pooled samples is reliable when at least 50% of the feces in a pool are from positive animals. However, since S. vulgaris is relatively rare in managed horses, using a pooled sample screening approach may result in some loss of diagnostic sensitivity. Nonetheless, pooled sample screening on farms could still be useful under certain circumstances, with PCR performing better at lower proportions of positive feces.
VETERINARY PARASITOLOGY
(2021)
Article
Parasitology
Christian M. Leutenegger, Cecilia E. Lozoya, Jeffrey Tereski, Jan Andrews, Kelly D. Mitchell, Cathy Meeks, Jennifer L. Willcox, Gregory Freeman, Holly L. Richmond, Christian Savard, Michelle D. Evason
Summary: This study compared the performance of real-time PCR (qPCR) and zinc sulfate centrifugal fecal flotation microscopy (ZCF) for fecal parasite screening. The results showed that qPCR had a significantly higher detection frequency than ZCF and could detect parasite markers that were undetected by ZCF.
PARASITES & VECTORS
(2023)
Article
Infectious Diseases
A. S. Muadica, S. Balasegaram, K. Beebeejaun, P. C. Koster, B. Bailo, M. Hernandez-de-Mingo, A. Dashti, E. Dacal, J. M. Saugar, I Fuentes, D. Carmena
Summary: The study found a high prevalence of intestinal parasites in children regardless of symptoms. Drinking well or river water, contact with domestic animals, and lack of latrines were contributing factors to human infections.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Microbiology
Ofelia Luis Nhambirre, Idalecia Cossa-Moiane, Adilson Fernando Loforte Bauhofer, Assucenio Chissaque, Maria Luisa Lobo, Olga Matos, Nilsa de Deus
Summary: Diarrhea remains a public health problem in Mozambique, and this study aimed to determine the proportion and factors associated with intestinal parasitic infection (IPI) in children with diarrheal disease. The most common parasites observed were Cryptosporidium spp. and the co-infection of Ascaris lumbricoides and Trichuris trichiura. Age and province were found to be associated with IPI.
Article
Medicine, General & Internal
Siriporn Srirungruang, Buraya Mahajindawong, Panachai Nimitpanya, Uthaitip Bunkasem, Pattama Ayuyoe, Surang Nuchprayoon, Vivornpun Sanprasert
Summary: This study assessed effective DNA extraction methods for different types of intestinal parasites and found that QB is the most effective method for the diagnosis and monitoring of intestinal parasites via PCR.
Article
Agriculture, Dairy & Animal Science
Xavier Blasco, Xavier Manteca, Manel Lopez-Bejar, Anais Carbajal, Joaquim Castella, Anna Ortuno
Summary: This study aimed to assess the impact of housing conditions on intestinal parasites and fecal cortisol metabolite levels in multi-unowned-cat environments. It was found that improving housing, such as increasing enclosure size and avoiding dog exposure, can reduce stress and the occurrence of intestinal parasites, especially protozoa.
Article
Microbiology
Veronica Casmo, Sergio Chicumbe, Rosa Chambisse, Rassul Nala
Summary: Intestinal parasitic infections (IPIs) are a public health problem among army recruits in southern Mozambique. The study found a prevalence of IPIs of 25.1%, with Entamoeba coli and Trichuris trichiura being the most common parasites. Parasitic infection was associated with the origin of the participant and the province of Sofala had the highest prevalence.
Article
Parasitology
Gustavo Henrique Sinhorin, Ana Carolina Gomes Carneiro, Beatrice Emeli Silva Farias, Patricia de Almeida, Antonio Ralph Medeiros-Sousa, Leonardo Augusto Kohara Melchior, Andreia Fernandes Brilhante
Summary: Intestinal parasite infections are a significant public health problem in the Amazon region, particularly during the dry season. Male and older individuals have a higher risk of infection, and certain parasites show a higher prevalence during the dry season compared to the rainy season.
PARASITOLOGY RESEARCH
(2023)
Article
Immunology
Ian Kim B. Tabios, Marcello Otake Sato, Ourlad Alzeus G. Tantengco, Raffy Jay C. Fornillos, Masashi Kirinoki, Megumi Sato, Raniv D. Rojo, Ian Kendrich C. Fontanilla, Yuichi Chigusa, Paul Mark B. Medina, Mihoko Kikuchi, Lydia R. Leonardo
Summary: Schistosomiasis still has a significant impact on public health in the Philippines. The commonly used Kato-Katz (K-K) technique has low sensitivity, especially in low endemic areas and patients with light infection. This study compared the diagnostic performance of immunological, molecular, parasitological, and ultrasonographic tests, and found that SjcDNA detection assay and POC-CCA urine test were more sensitive than stool microscopy in detecting schistosomiasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Junxia Feng, Xiaohu Cui, Bing Du, Hanqing Zhao, Yanling Feng, Jinghua Cui, Chao Yan, Lin Gan, Zheng Fan, Tongtong Fu, Ziying Xu, Zihui Yu, Rui Zhang, Shuheng Du, Ziyan Tian, Qun Zhang, Guanhua Xue, Jing Yuan
Summary: This study developed a rapid and sensitive ddPCR assay for detecting Klebsiella pneumoniae in fecal samples. The ddPCR assay showed higher sensitivity, specificity, and lower inhibition compared to real-time PCR and microbial culture. The findings suggest that ddPCR could be an effective tool for detecting K. pneumoniae in fecal samples and guiding treatment decisions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Joao Lozano, Mariana Louro, Cristina Almeida, Ana Claudia Victorio, Pedro Melo, Joao Paulo Rodrigues, Manuela Oliveira, Adolfo Paz-Silva, Luis Madeira de Carvalho
Summary: This study isolated seven Mucor isolates from avian fecal samples, which is the first report of native predatory fungi isolated from avian feces and demonstrated their lytic activity against coccidia.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Xueyong Zhang, Yingna Jian, Yijuan Ma, Zhi Li, Yong Fu, Zhouzai Cairang, Xiaohong Wang, Hong Duo, Zhihong Guo
Summary: The study found that the prevalence of intestinal parasite infections in dogs in urban and rural environments in Qinghai Province differed, with higher rates in rural areas, and some parasites were only found in rural environments. Dogs are important sources of environmental contamination, posing a potential zoonotic infection risk to humans and other animals.
Article
Microbiology
Catia Salamandane, Maria Luisa Lobo, Sonia Afonso, Regina Miambo, Olga Matos
Summary: Important pathogenic intestinal parasites such as Giardia duodenalis, Cryptosporidium, and Enterocytozoon bieneusi were identified in fruits/vegetables commercially purchased in Maputo City. Measures should be taken to reduce the risk of contamination of fresh produce by intestinal parasites and prevent foodborne diseases in the management of these horticultural markets.
Article
Microbiology
Aly S. Muadica, Pamela C. Koster, Alejandro Dashti, Begona Bailo, Marta Hernandez-de-Mingo, Sooria Balasegaram, David Carmena
Summary: The study found that Giardia duodenalis was the most common enteric parasite in children in Mozambique, followed by Blastocystis sp. and Cryptosporidium spp. Various species and subtypes of these parasites were detected in the children, indicating complex transmission pathways.
Article
Multidisciplinary Sciences
Adam Diedrich, Mano Sivaganesan, Jessica R. Willis, Amirreza Sharifi, Orin C. Shanks
Summary: Municipal stormwater systems are designed to collect, transport, and discharge precipitation from a defined catchment area into local surface waters. However, these discharges may contain unsafe levels of fecal waste.
Article
Public, Environmental & Occupational Health
Lynn Meurs, Felix S. Lempp, Norman Lippmann, Henning Trawinski, Arne C. Rodloff, Matthias Eckardt, Anja Klingeberg, Tim Eckmanns, Jan Walter, Christoph Luebbert, Muna Abu Sin, Esther-Maria Antao, Michael Behnke, Jutta Bleidorn, Evgeniya Boklage, Christina Forstner, Petra Gastmeier, Jochen Gensichen, Alexander Gropmann, Stefan Hagel, Regina Hanke, Wolfgang Hanke, Lukas Klimmek, Ulrich Kraft, Tobias Kramer, Markus Lehmkuhl, Norman Ludwig, Antina Luebke-Becker, Oliwia Makarewicz, Anne Moeser, Inga Petruschke, Mathias W. Pletz, Cornelius Remschmidt, Florian Salm, Katja Schmuecker, Sandra Schneider, Christin Schroeder, Frank Schwab, Joachim Trebbe, Szilvia Vincze, Horst C. Vollmar, Sebastian Weis, Wibke Wetzker, Lothar H. Wieler
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2020)
Article
Immunology
Lucja A. Labuda, Ayola A. Adegnika, Bruce A. Rosa, John Martin, Ulysse Ateba-Ngoa, Abena Serwaa Amoah, Honorine Mbenkep Lima, Lynn Meurs, Moustapha Mbow, Mikhael D. Manurung, Jeannot F. Zinsou, Hermelijn H. Smits, Peter G. Kremsner, Makedonka Mitreva, Maria Yazdanbakhsh
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Multidisciplinary Sciences
Joep J. J. M. Stohr, Jaco J. Verweij, Anton G. M. Buiting, John W. A. Rossen, Jan A. J. W. Kluytmans
Article
Parasitology
Thomas Koeller, Andreas Hahn, Enkhtsetseg Altangerel, Jaco J. Verweij, Olfert Landt, Simone Kann, Denise Dekker, Juergen May, Ulrike Loderstaedt, Andreas Podbielski, Hagen Frickmann
Article
Infectious Diseases
Reina S. Sikkema, Suzan D. Pas, David F. Nieuwenhuijse, Aine O'Toole, Jaco Verweij, Anne van der Linden, Irina Chestakova, Claudia Schapendonk, Mark Pronk, Pascal Lexmond, Theo Bestebroer, Ronald J. Overmars, Stefan van Nieuwkoop, Wouter van den Bijllaardt, Robbert G. Bentvelsen, Miranda M. L. van Rijen, Anton G. M. Buiting, Anne J. G. van Oudheusden, Bram M. Diederen, Anneke M. C. Bergmans, Annemiek van der Eijk, Richard Molenkamp, Andrew Rambaut, Aura Timen, Jan A. J. W. Kluytmans, Bas B. Oude Munnink, Marjolein F. Q. Kluytmans van den Bergh, Marion P. G. Koopmans
LANCET INFECTIOUS DISEASES
(2020)
Article
Biochemistry & Molecular Biology
Bas B. Oude Munnink, David F. Nieuwenhuijse, Mart Stein, Aine O'Toole, Manon Haverkate, Madelief Mollers, Sandra K. Kamga, Claudia Schapendonk, Mark Pronk, Pascal Lexmond, Anne van der Linden, Theo Bestebroer, Irina Chestakova, Ronald J. Overmars, Stefan van Nieuwkoop, Richard Molenkamp, Annemiek A. van der Eijk, Corine GeurtsvanKessel, Harry Vennema, Adam Meijer, Andrew Rambaut, Jaap van Dissel, Reina S. Sikkema, Aura Timen, Marion Koopmans
Article
Infectious Diseases
Piet Cools, Lisette van Lieshout, Rob Koelewijn, David Addiss, Sitara S. R. Ajjampur, Mio Ayana, Richard S. Bradbury, Jason L. Cantera, Daniel Dana, Kerstin Fischer, Rubina Imtiaz, Joyce Kabagenyi, James Lok, James McCarthy, Rojelio Mejia, Zeleke Mekonnen, Sammy M. Njenga, Nurulhasanah Othman, Hongguang Shao, Rebecca Traub, Marjan Van Esbroeck, Jozef Vercruysse, Johnny Vlaminck, Steven A. Williams, Jaco J. Verweij, Jaap J. van Hellemond, Bruno Levecke
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Infectious Diseases
Bruno Levecke, Piet Cools, Marco Albonico, Shaali Ame, Cecile Angebault, Mio Ayana, Jerzy M. Behnke, Jeffrey M. Bethony, Giuseppe Cringoli, Daniel Dana, Bertrand Guillard, Nguyen Thi Viet Hoa, Gagandeep Kang, Deepthi Kattula, Jennifer Keiser, Andrew C. Kotze, Leonardo F. Matoso, Maria P. Maurelli, James S. McCarthy, Zeleke Mekonnen, Greg Mirams, Antonio Montresor, Rodrigo Correa Oliveira, Maria V. Periago, Simone A. Pinto, Laura Rinaldi, Somphou Sayasone, Laurentine Sumo, Louis-Albert Tchuem-Tchuente, Dang Thi Cam Thach, Eurion Thomas, Ahmed Zeynudin, Jaco J. Verweij, Johnny Vlaminck, Jozef Vercruysse
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Virology
Eric C. J. Claas, Pieter W. Smit, Mario J. A. W. M. van Bussel, Harold Verbakel, Mohammed Taouil, Jaco J. Verweij, Steven F. T. Thijsen
Summary: The QIA P&A kit shows good performance in sample preparation and amplification of SARS-CoV-2 and can serve as a rapid molecular testing strategy for managing the pandemic.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Infectious Diseases
Piet Cools, Johnny Vlaminck, Jaco J. Verweij, Bruno Levecke
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Microbiology
Tanja Hoffmann, Andreas Hahn, Jaco J. Verweij, Gerard Leboulle, Olfert Landt, Christina Strube, Simone Kann, Denise Dekker, Jurgen May, Hagen Frickmann, Ulrike Loderstadt
Summary: This study compared standard and harsh nucleic acid extraction methods for helminth real-time PCR, finding that in some cases, harsh extraction methods may increase the positivity rate of test results, but the effects vary depending on the specific real-time PCR assay used.
Article
Pathology
Jaco J. Verweij
Summary: This paper discusses setting tailor-made performance characteristics for the validation of laboratory developed tests (LDTs) for the molecular diagnosis of intestinal parasitic infections in compliance with ISO 15189 guidelines, emphasizing the importance of maintaining these tests through daily monitoring and reassessment of the test set-up. It highlights the necessity of modifying or adding additional characteristics based on personal insights to achieve continuous quality improvement in diagnostic laboratory practice.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2022)
Article
Virology
N. Van der Moeren, V. F. Zwart, G. Goderski, G. T. Rijkers, W. van den Bijllaardt, J. Veenemans, J. A. J. W. Kluytmans, S. D. Pas, A. Meijer, J. J. Verweij, J. L. A. N. Murk, J. J. J. M. Stohr
Summary: The study evaluated the performance of the Diasorin SARS-CoV-2 antigen detection assay (DAA) in clinical and experimental samples, demonstrating high sensitivity and specificity in clinical samples with a Ct value below 30. The test has potential for use as a diagnostic assay for symptomatic community-dwelling individuals early after disease onset in the context of disease control.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Virology
R. Jajou, A. J. G. Mutsaers-van Oudheusden, J. J. Verweij, A. Rietveld, J. L. Murk
Summary: This study found that individuals who transmitted SARS-CoV-2 had viral loads more than three times higher than non-transmitters. The information of viral load can be used to prioritize individuals with the highest risk of transmission during source and contact tracing.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Microbiology
Nathalie Van der Moeren, Veronica A. T. C. Weterings, Suzan D. Pas, Jaco J. Verweij, Wouter van den Bijllaardt, Joyce Geurts, Vivian F. Zwart, Esther B. Lodder, Jan A. J. W. Kluytmans, Jean-Luc Murk, Joep J. J. M. Stohr
Summary: This study retrospectively describes a SARS-CoV-2 outbreak among a partially vaccinated population in a long-term care facility in the Netherlands. The results suggest that phylogenetic analysis, with a maximum number of included samples, can help control such outbreaks. The study also suggests that long-term care facilities may behave as a single epidemiological unit.