Article
Biochemistry & Molecular Biology
Feng Kong, Katrina M. Ramonell
Summary: The gene ATL12 plays an important role in the response to salt stress and abscisic acid (ABA) in Arabidopsis thaliana. Overexpression of ATL12 increases the expression of salt stress-associated genes and ABA-responsive genes, and leads to higher levels of reactive oxygen species. Mutants of atl12 are sensitive to salt stress and ABA, and have shorter root length. The findings suggest that ATL12 modulates the response to salt stress and is involved in the ABA signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mingshuo Du, Dongping Lu, Xiaotong Liu
Summary: The ATL31/6 genes are involved in positively regulating plant response to salt stress, independent of ABA, and participate in regulating plant response to both biotic and abiotic stress.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Plant Sciences
Syed Muhammad Muntazir Mehdi, Michal Wojciech Szczesniak, Agnieszka Ludwikow
Summary: Abscisic acid (ABA) affects plant physiology by regulating gene expression. The AtBro1 gene plays a crucial role in the plant's response to abiotic stress, such as drought and salt stress. ABA stimulates stress-resistance responses through the activation of AtBro1.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Jun Tang, Junbo Yang, Hongchao Duan, Guifang Jia
Summary: The epitranscriptomic mark N-6-methyladenine (m(6)A) regulates plant development and stress response. ALKBH10B, an mRNA m(6)A demethylase in Arabidopsis, was found to negatively modulate the ABA response during seed germination. Transcriptome analysis revealed upregulation of ABA response genes in the alkbh10b mutants, indicating a role of ALKBH10B in plant stress responses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Hai An Truong, Seokjin Lee, Cao Son Trinh, Won Je Lee, Eui-Hwan Chung, Suk-Whan Hong, Hojoung Lee
Summary: Histone modification, particularly HDA15 overexpression, plays a crucial role in enhancing salt stress resistance in plants by regulating stress-responsive gene expression and ABA levels. Interaction between HDA15 and HY5 is essential for salt stress tolerance in Arabidopsis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Maria Naranjo-Arcos, Moumita Srivastava, Florian Deligne, Prakash Kumar Bhagat, Mansi Mansi, Ari Sadanandom, Gregory Vert
Summary: This study reveals that the plant hormone receptor BRI1 is regulated by SUMOylation and its activity is controlled by the SUMO protease Desi3a. Moreover, Desi3a regulates the interaction between BRI1 and the negative regulator BIK1 as well as BRI1 endocytosis. The deSUMOylation of BRI1 by Desi3a is proposed as a molecular crosstalk mechanism between temperature and BR signaling, enabling plants to respond to environmental inputs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Jun Tang, Junbo Yang, Qiang Lu, Qian Tang, Shuyan Chen, Guifang Jia
Summary: The mRNA modification N-6-methyladenosine (m(6)A) plays vital roles in ABA responses, and ALKBH9B-mediated m(6)A demethylation is crucial for post-transcriptional regulation of ABA responses.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Ping-Xia Zhao, Jing Zhang, Si-Yan Chen, Jie Wu, Jing-Qiu Xia, Liang-Qi Sun, Shi-Song Ma, Cheng-Bin Xiang
Summary: AGL16 acts as a negative regulator in stress response in Arabidopsis, with loss-of-AGL16 conferring resistance to salt stress and elevated AGL16 expression leading to opposing phenotypes compared with wild-type. The sensitivity to ABA in seed germination is inversely correlated with AGL16 expression levels.
Article
Biochemistry & Molecular Biology
Yanan Chen, Ling Li, Boyan Tang, Ting Wu, Guoping Chen, Qiaoli Xie, Zongli Hu
Summary: Results show that SlMYB55 affects ABA biosynthesis, regulates drought and salt responses through ABA-mediated pathways, and impacts the expression of genes related to stress response, flowering time, sepal size, and inflorescence, ultimately influencing stress tolerance and flower development in plants.
Article
Plant Sciences
Yibin Wang, Gaofeng Zhang, Huimin Zhou, Shanshan Yin, Yunxiang Li, Caixia Ma, Pengyun Chen, Lirong Sun, Fushun Hao
Summary: The cotton ABA receptors GhPYL9-5D and GhPYR1-3A are found to play important roles in response to ABA and abiotic stresses. Overexpression of these genes in Arabidopsis enhances ABA sensitivity and increases tolerance to drought, salt, and osmotic stress. Knockdown of these genes in cotton leads to reduced tolerance to polyethylene glycol 6000-induced drought, salinity, and osmotic stresses. In addition, these genes are co-expressed with redox signaling components, transcription factors, and auxin signal components, suggesting their involvement in multiple signaling pathways.
Article
Plant Sciences
An Shao, Xiao Xu, Erick Amombo, Wei Wang, Shugao Fan, Yanling Yin, Xiaoning Li, Guangyang Wang, Hongli Wang, Jinmin Fu
Summary: English Summary: Common bermudagrass has great potential for utilization on saline soil due to its high yield potential and excellent stress tolerance. CdWRKY2, when overexpressed in bermudagrass and Arabidopsis, enhances salt and ABA sensitivity, leading to inhibition of shoot and root growth. This study reveals that CdWRKY2 negatively regulates lateral root growth under salt stress by modulating ABA signaling and auxin homeostasis, partly dependent on AtMAPK3 function. CdWRKY2 and its homologue genes could serve as targets for genetic engineering of salinity-tolerant plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Shuangshuang Zhao, Qikun Zhang, Mingyue Liu, Huapeng Zhou, Changle Ma, Pingping Wang
Summary: Plants adapt to high-salt environments by regulating ion balance, activating osmotic stress pathways, mediating plant hormone signaling, and adjusting cytoskeleton dynamics and cell wall composition in response to salt stress signals. Understanding these mechanisms is crucial for improving agricultural crop yields.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Liang Ma, Xiaohong Liu, Wanjia Lv, Yongqing Yang
Summary: Saline-alkali soils pose a serious threat to plant growth and productivity worldwide. Scientists have made significant progress in understanding how plants adapt to salt stress by regulating ion homeostasis. The primary goals of plant salt stress signaling research in recent decades have been to understand the molecular mechanisms affecting salt tolerance and develop strategies to breed salt-resistant crops. This review focuses on recent advances in our understanding of the cellular and physiological mechanisms involved in plant responses to salt stress, particularly the changes in signal perception, decoding, and transduction in specific organelles or cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yanjun He, Yue Liu, Mengzhuo Li, Anthony Tumbeh Lamin-Samu, Dandan Yang, Xiaolin Yu, Muhammad Izhar, Ibadullah Jan, Muhammad Ali, Gang Lu
Summary: AtSAUR32, a member of the SAUR family, plays a crucial role in drought stress adaptation in Arabidopsis by mediating ABA signal transduction pathways.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Cell Biology
Waqar Islam, Abdul Waheed, Hassan Naveed, Fanjiang Zeng
Summary: Soil salinity is one of the most damaging factors to cultivatable land. Salt stress negatively affects plant growth and yields, and severe stress can be harmful. Plants have developed mechanisms, such as miRNA-mediated gene regulation, to withstand salt stress and other environmental variables. This review article discusses the adaptations of various plant species to salt stress through miRNA, as well as the salt responsive miRNAs, their target genes, and pathways. The article concludes that utilizing miRNAs may be a vital strategy for generating salt tolerant crops and ensuring food security in the future.
Review
Biochemistry & Molecular Biology
Leia Colin, Felix Ruhnow, Jian-Kang Zhu, Chunzhao Zhao, Yang Zhao, Staffan Persson
Summary: Salt stress causes ionic toxicity, osmotic stress, and oxidative stress, directly affecting plant growth and development. Plants perceive salt stress through the cell wall and coordinate effective responses. Some mechanisms underlying these processes have been elucidated.
Article
Chemistry, Analytical
Xin Feng, Anton Iliuk, Xiaoye Zhang, Shengnan Jia, Ao Shen, Wenke Zhang, Lianghai Hu, W. Andy Tao
Summary: In this study, an amphiphile-dendrimer supramolecular probe (ADSP) was developed for efficient capture and high-throughput analysis of exosomes, allowing the detection of marker proteins. The ADSP forms a supramolecular complex with exosomes by interacting with their phospholipid bilayers, and can be easily coated onto magnetic beads or nitrocellulose membranes for capture. This new strategy exhibits excellent extraction capability, superior sensitivity, good quantitation ability, and high throughput using clinical samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Xiangxiang Meng, Quanhui Wang, Ruili Hao, Xudong Li, Mu Li, Ruibo Hu, Hai Du, Zhubing Hu, Bin Yu, Shengjun Li
Summary: DNA damage response (DDR) is crucial for maintaining genome integrity in plants. This study reveals the role of MAC5A in DDR and its interaction with the 26S proteasome in Arabidopsis. MAC core subunits and the 26S proteasome are also involved in MMS-induced DDR. These findings contribute to a better understanding of the regulatory mechanisms of DDR in plants and its importance in growth and stress adaptation.
Article
Plant Sciences
Mingjing Wang, Hailong Zhang, Xiaonan Zhao, Jingwen Zhou, Guochen Qin, Yuqi Liu, Xiaoyue Kou, Zhenjie Zhao, Tao Wu, Jian-Kang Zhu, Xianzhong Feng, Lixin Li
Summary: The gene AtSYP81 in Arabidopsis thaliana plays a critical role in root growth and development by regulating peroxisome- and peroxidase-mediated ROS homeostasis. Knockdown of AtSYP81 resulted in reduced root meristem activity and disruption of root stem cell niche identity. Transcriptome analysis revealed changed expression of class III peroxidases, adding evidence of the regulatory role of AtSYP81 in ROS signaling.
Article
Chemistry, Multidisciplinary
Jie Sun, Qing Li, Yajie Ding, Dong Wei, Marco Hadisurya, Zhuojun Luo, Zhongze Gu, Bobin Chen, W. Andy Tao
Summary: Researchers introduced a functionally tunable material and strategy, extracellular vesicles to phosphoproteins (EVTOP), for monitoring disease-relevant phosphorylation events in circulating biofluids. EVs were efficiently isolated by magnetic beads and enriched for phosphopeptides for phosphoproteomic analyses. This platform enables the quantitative detection of a large number of EV phosphopeptides in a small volume of biofluids, presenting broad clinical applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Editorial Material
Biochemistry & Molecular Biology
Andres J. Cortes, Hai Du
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Xin Feng, Shengnan Jia, Muhammad Mujahid Ali, Guiyuan Zhang, Dejun Li, W. Andy Tao, Lianghai Hu
Summary: This study analyzed the urinary exosomal proteome and performed high-throughput downstream validation using a supramolecular probe-based capture and in situ detection. The technology efficiently enriched exosomes from urine samples with high concentration (5.5 x 1010 particles/mL) and high purity (2.607 x 1010 particles/mg). Proteomic analysis revealed 68 up-regulated proteins in patients with hepatocellular carcinoma, and three of them were validated using the supramolecular probe-based array.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Marco Hadisurya, Zheng-Chi Lee, Zhuojun Luo, Guiyuan Zhang, Yajie Ding, Hao Zhang, Anton B. Iliuk, Roberto Pili, Ronald S. Boris, W. Andy Tao
Summary: The translation of cancer signaling research into clinical application has been slow and ineffective. However, extracellular vesicles (EVs) show promise as a source for developing disease phosphoprotein markers. This study focuses on using mass spectrometry to profile urinary EV phosphoproteomics for renal cell cancer (RCC) differentiation. By optimizing the process of EV isolation, phosphopeptide enrichment, and DIA method, this study provides a powerful tool for future clinical applications.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Nan Wang, Yao Deng, Lisha Zhang, Yingchun Wan, Ting Lei, Yimin Yang, Can Wu, Hai Du, Ping Feng, Wuzhong Yin, Guanghua He
Summary: A rice mutant, osuge1, lacking UDP-glucose epimerase activity, exhibited delayed tapetum degradation and abnormal pollen development. OsUGE1 was found to participate in the TIP2/bHLH142-TDR-EAT1/DTD transcriptional regulatory cascade, in which TIP2 and TDR regulate OsUGE1 expression while OsUGE1 regulates EAT1 expression. Moreover, OsUGE1 was shown to regulate its own gene expression by directly binding to an E-box element in its promoter. These findings demonstrate that OsUGE1 has moonlighting functions as a transcriptional activator in addition to its role as a UDP-glucose epimerase.
Correction
Biochemistry & Molecular Biology
Ammara Abdullah, Saeed Salehin Akhand, Juan Sebastian Paez Paez, Wells Brown, Li Pan, Sarah Libring, Michael Badamy, Emily Dykuizen, Luis Solorio, W. Andy Tao, Michael K. Wendt
Review
Chemistry, Analytical
Xiaofeng Wu, Yi-Kai Liu, Anton B. Iliuk, W. Andy Tao
Summary: Protein phosphorylation is crucial for cellular physiology and dysregulation of phosphorylation events can lead to diseases. Clinical analysis of disease-related phosphoproteins provides unique information for precision medicine. Mass spectrometry (MS) is a powerful tool for identifying and studying phosphorylation events, and recent advances in sample preparation and instrument technology have contributed to its clinical applications. Data-independent acquisition method in MS and biofluid-derived extracellular vesicles are promising directions for future research and liquid biopsy.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yi-Kai Liu, Xiaofeng Wu, Marco Hadisurya, Li Li, Hristos Kaimakliotis, Anton Iliuk, W. Andy Tao
Summary: Extracellular vesicle proteomics is a useful tool for discovering biomarkers for disease diagnosis and treatment. However, the current workflow for analyzing EV proteomes using mass spectrometry is not suitable for clinical settings. In this study, we developed a one-pot analytical pipeline that combines a robust EV isolation method and in situ protein sample preparation to detect urinary EV proteomes. This streamlined pipeline enabled the analysis of the entire EV proteome in one day and yielded better results compared to existing methods.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Zheng-Chi Lee, Marco Hadisurya, Zhuojun Luo, Li Li, W. Andy Tao
Summary: Extracellular vesicles (EVs) have been identified as a promising source of disease biomarkers. However, the current methods for processing EV samples have limitations in terms of yield, integrity, speed, capacity, and recovery efficiency. In this study, we developed a high-throughput automated workflow that enables efficient isolation, lysis, and extraction of proteins from EVs in clinical samples. The automated protocol shows reproducible and robust proteomic quantitation, and has been successfully applied to identify upregulated proteins in kidney cancer patients compared to healthy controls. This hands-free workflow has the potential to streamline biomarker discovery, tumor monitoring, and early cancer diagnoses in both clinical and research settings.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Plant Sciences
Xiaoran Yang, Ran Hu, Fujun Sun, Shulin Shen, Mengzhen Zhang, Yiwei Liu, Yi Zhang, Hai Du, Kun Lu, Cunmin Qu, Nengwen Yin
Summary: This study identified and characterized HKT genes in rapeseed, revealing their roles in response to abiotic stresses. Based on phylogenetic relationships and functional domain composition, the HKT members were classified into four subgroups. The expression profiles of BnaHKT genes varied under potassium, phytohormone, and heavy-metal treatment, suggesting their involvement in heavy-metal tolerance.
Article
Biochemical Research Methods
Zheng-Chi Lee, Marco Hadisurya, Zhuojun Luo, Li Li, W. Andy Tao
Summary: This study developed an automated workflow for efficient and reproducible isolation and protein analysis of extracellular vesicles (EVs), overcoming the bottleneck in EV sample processing. The method identified a large number of potential biomarkers in kidney cancer patients compared to healthy controls, demonstrating its potential for biomarker discovery and early cancer diagnoses.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)