Article
Biochemistry & Molecular Biology
Daniella Souza Silva, William Marcondes Facchinatto, Danilo Martins dos Santos, Fernanda Isadora Boni, Talita Alvarenga Valdes, Andrei Leitao, Maria Palmira Daflon Gremiao, Luiz Alberto Colnago, Sergio Paulo Campana-Filho, Sidney Jose Lima Ribeiro
Summary: Two samples of DPCat derivatives with different degrees of quaternization were successfully synthesized and showed good water solubility and electrostatic stability at different pH levels. DPCat35 demonstrated better interactions with biological tissue and mucin glycoproteins, suggesting potential for the development of drug delivery systems for prostate and liver cancer treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Ali Dehshahri, Bahman Khalvati, Zahra Taheri, Farshad Safari, Reza Mohammadinejad, Abolfazl Heydari
Summary: This study evaluated the transfection efficiency and cytotoxicity of N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan salt (HTCS) as a gene carrier. HTCS was synthesized and used for the delivery of the interleukin-12 (IL-12) gene plasmid. The results showed that HTCS could effectively condense the IL-12 plasmid into polyplexes and demonstrated higher hIL-12 production compared to CS and polyethylenimine polyplexes in cell transfection experiments.
Article
Engineering, Biomedical
William Marcondes Facchinatto, Joana Galante, Leticia Mesquita, Daniella Souza Silva, Danilo Martins dos Santos, Tiago Bueno Moraes, Sergio Paulo Campana-Filho, Luiz Alberto Colnago, Bruno Sarmento, Jose das Neves
Summary: This study explored the use of novel nanoparticles as carriers for clotrimazole, demonstrating good drug release properties and improved drug permeability in cell monolayer models.
ACTA BIOMATERIALIA
(2021)
Article
Polymer Science
Marina Gorshkova, Irina Volkova
Summary: The interaction between HTCC and ASS is pH-dependent, resulting in stable complexes suitable for the development of biocompatible, biodegradable, and pH-tunable drug delivery systems. Factors such as the ratio of components, molecular mass, pH, and salt concentration must be considered when preparing such complexes.
JOURNAL OF POLYMER RESEARCH
(2021)
Article
Chemistry, Applied
Yong Li, Yan Li, Tao Chen, Xiaodeng Yang, Congde Qiao, Fei Hao, Mingxia Liu
Summary: This paper investigates the effect of crystallized calcium carbonate particles (cry-CaCO3) on the properties of HTCC/CMC films. The results show that cry-CaCO3 particles promote the homogeneous distribution of the HTCC/CMC matrix, significantly improving mechanical properties while having little effect on thermal stability, whiteness, and wettability of the films. The study further characterizes the cry-CaCO3 particles and reveals that the strong electrostatic, hydrogen bonding, and flexible interaction between CMC and cry-CaCO3 particles play a key role in improving the mechanical properties of HTCC/CMC films.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Danyang Wang, Yongjuan Zou, Ning Wang, Jie Wu
Summary: We developed an emulsion using chitosan hydrochloride salt (CSCL) as stabilizer, which effectively loaded antigens and slowed down antigen release. The emulsion showed strong immune responses and generated memory T cells in adaptive immunity.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Min Wang, Lin Yue, Sobia Niazi, Imran Mahmood Khan, Yin Zhang, Zhouping Wang
Summary: The study synthesized a novel hybrid flocculant with antibacterial activity, HTCC-CA, by conjugating cinnamic acid with HTCC. The results showed that HTCC-CA derivatives exhibited excellent flocculation efficiency and high antibacterial activity, making them promising agents for wastewater treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Chi-Chieh Chang, Abdullah Algaissi, Chia-Chun Lai, Chun-Kai Chang, Jr-Shiuan Lin, Yi-Shiang Wang, Bo-Hau Chang, Yu-Chiuan Chang, Wei-Ting Chen, Yong-Qing Fan, Bi-Hung Peng, Chih-Yu Chao, Shiou-Ru Tzeng, Pi-Hui Liang, Wang-Chou Sung, Alan Yung-Chih Hu, Shin C. Chang, Ming-Fu Chang
Summary: Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks cause high mortality and require the development of an effective vaccine. The trimeric spike protein on the viral envelope plays a crucial role in MERS-CoV infection. A subunit vaccine containing the recombinant S1 protein was designed and proved to induce strong immune responses. The use of the QS-21 adjuvant enhanced humoral and cellular immunity in mice, suggesting its potential as an effective adjuvant for MERS-CoV vaccines.
Article
Immunology
Kai Zhao, Yuan Gao, Gaowei Hu, Lei Wang, Shangjin Cui, Zheng Jin
Summary: The study developed a VP2 protein subunit vaccine against Porcine parvovirus (PPV) using N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) as the adjuvant, showing that the vaccine induced higher antibody levels and long-term immune protection in immunized sows. However, the vaccine mainly stimulated humoral immunity rather than cellular immunity, and provided 100% protective efficacy against PPV infection in sows. This suggests that N-2-HACC could be a potential adjuvant for controlling PPV.
Article
Immunology
Naomi Nacasch, Keren Cohen-Hagai, Nurit Tayar, Avraham Levian, Gloria Rashid, Sydney Benchetrit, Eran Neumark, Ori Wand, Tammy Hod, Yossi Rosman, Moshe Shashar, Ayelet Grupper, Pnina Shitrit
Summary: This study compared the humoral response to HBV and COVID-19 vaccines in MHD patients, finding that a reduced response to hepatitis B was not associated with a reduced response to COVID-19 vaccine. Different risk factors were associated with poor immune response to HBV and to COVID-19 vaccines.
Article
Nanoscience & Nanotechnology
Yuhong Lin, Beini Sun, Zheng Jin, Kai Zhao
Summary: N-2-hydroxypropyl trimethyl ammonium chloride chitosan/N,O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) were prepared and demonstrated to have excellent mucosal absorption and enhanced immune effects. These nanoparticles could promote lymphocyte proliferation and the secretion of pro-inflammatory factors, leading to stimulation of mucosal and systemic immune responses. Chitosan-based nano-delivery system has great promise as a mucosal vaccine adjuvant in viral infectious diseases and immunization therapy.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Adam M. Weiss, Jainu Ajit, Tyler J. Albin, Neeraj Kapoor, Shilpa Maroju, Aym Berges, Lucy Pill, Jeff Fairman, Aaron P. Esser-Kahn
Summary: This study demonstrated the successful use of CFPS technology to produce large quantities of site-specific antigen-adjuvant conjugates for enhanced antigen presentation and T-cell production. Site-specific conjugation using copper-free click chemistry significantly improved the efficacy of the OVA-CpG conjugates both in vitro and in vivo, reducing the required CpG dose for T-cell production tenfold.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Isra Dmour, Nazrul Islam
Summary: Chitosan is a natural polymer with wide applications in the field of drugs, vaccines, and antigen delivery. It has unique properties that make it a promising adjuvant in vaccine delivery. Studies have shown that nanoparticles loaded with chitosan and its derivatives can induce immune responses. The physicochemical properties of chitosan provide an excellent opportunity for engineering antigen-specific adjuvant/delivery systems.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Virology
Felicia Schlotthauer, Joey McGregor, Heidi E. Drummer
Summary: Despite the high effectiveness of direct-acting antiviral agents in treating existing hepatitis C infections, most countries are unlikely to reach the World Health Organization targets for elimination of HCV by 2030. Therefore, the development of a prophylactic vaccine remains a top priority. Current research is shifting towards vaccines that generate humoral immunity, either alone or in combination with T cell-based vaccines, with a focus on high-resolution structures of viral glycoproteins and their interaction with monoclonal antibodies.
Article
Immunology
Bruno J. Lewin, Janelle Rodriguez, Su-Jau Yang, Sara Y. Tartof
Summary: This study found that individuals with Hispanic and Asian/Pacific Islander backgrounds, as well as those who preferred a language other than English, had a higher prevalence of Hepatitis A immunity.
