Advancements in DNA vaccine vectors, non-mechanical delivery methods, and molecular adjuvants to increase immunogenicity

Crosstalk between Cytoplasmic RIG-I and STING Sensing Pathways

(2017) Alessandra Zevini et al.   TRENDS IN IMMUNOLOGY

An Interleukin 12 Adjuvanted Herpes Simplex Virus 2 DNA Vaccine Is More Protective Than a Glycoprotein D Subunit Vaccine in a High-Dose Murine Challenge Model

(2017) Kenneth C. Bagley et al.   VIRAL IMMUNOLOGY

Cutting Edge: The RIG-I Ligand 3pRNA Potently Improves CTL Cross-Priming and Facilitates Antiviral Vaccination

(2016) Katharina Hochheiser et al.   JOURNAL OF IMMUNOLOGY

Triggering Intracellular Receptors for Vaccine Adjuvantation

(2016) Alice Gutjahr et al.   TRENDS IN IMMUNOLOGY

Effects of Circular DNA Length on Transfection Efficiency by Electroporation into HeLa Cells

(2016) Benjamin D. Hornstein et al.   PLoS One

Towards effective non-viral gene delivery vector

(2015) Michaela Šimčíková et al.   Biotechnology & Genetic Engineering Reviews

A cGAS-Independent STING/IRF7 Pathway Mediates the Immunogenicity of DNA Vaccines

(2015) John J. Suschak et al.   JOURNAL OF IMMUNOLOGY

Enhanced Influenza Virus-Like Particle Vaccination with a Structurally Optimized RIG-I Agonist as Adjuvant

(2015) Vladimir Beljanski et al.   JOURNAL OF VIROLOGY

Co-administration of plasmid-encoded granulocyte-macrophage colony-stimulating factor increases human immunodeficiency virus-1 DNA vaccine-induced polyfunctional CD4+ T-cell responses

(2015) Vinicius Canato Santana et al.   MEMORIAS DO INSTITUTO OSWALDO CRUZ

Co-administration with DNA encoding papillomavirus capsid proteins enhances the antitumor effects generated by therapeutic HPV DNA vaccination

(2015) Benjamin Yang et al.   Cell and Bioscience

Vector optimization and needle-free intradermal application of a broadly protective polyvalent influenza A DNA vaccine for pigs and humans

(2015) Marie Borggren et al.   Human Vaccines & Immunotherapeutics

Codon-optimized filovirus DNA vaccines delivered by intramuscular electroporation protect cynomolgus macaques from lethal Ebola and Marburg virus challenges

(2015) Rebecca J Grant-Klein et al.   Human Vaccines & Immunotherapeutics

Innate Immune Sensing and Signaling of Cytosolic Nucleic Acids

(2014) Jiaxi Wu et al.   Annual Review of Immunology

Protective efficacy of Toxoplasma gondiicalcium-dependent protein kinase 1 (TgCDPK1) adjuvated with recombinant IL-15 and IL-21 against experimental toxoplasmosis in mice

(2014) Jia Chen et al.   BMC INFECTIOUS DISEASES

Improving DNA Vaccine Performance Through Vector Design

(2014) James Williams   CURRENT GENE THERAPY

Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells

(2014) C Bergamaschi et al.   GENE THERAPY

Variable effects of the co-administration of a GM-CSF-expressing plasmid on the immune response to flavivirus DNA vaccines in mice

(2014) Hui Chen et al.   IMMUNOLOGY LETTERS

CpG/Poly (I:C) mixed adjuvant priming enhances the immunogenicity of a DNA vaccine against eastern equine encephalitis virus in mice

(2014) JinZhu Ma et al.   INTERNATIONAL IMMUNOPHARMACOLOGY

Therapeutic Vaccination with Simian Immunodeficiency Virus (SIV)-DNA+IL-12 or IL-15 Induces Distinct CD8 Memory Subsets in SIV-Infected Macaques

(2014) R. Halwani et al.   JOURNAL OF IMMUNOLOGY

Identification of Aim2 as a Sensor for DNA Vaccines

(2014) John J. Suschak et al.   JOURNAL OF IMMUNOLOGY

Activation of the RIG-I Pathway during Influenza Vaccination Enhances the Germinal Center Reaction, Promotes T Follicular Helper Cell Induction, and Provides a Dose-Sparing Effect and Protective Immunity

(2014) R. R. Kulkarni et al.   JOURNAL OF VIROLOGY

Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice

(2014) Zhong-Yuan Li et al.   VACCINE

Microbial Sensing by Toll-Like Receptors and Intracellular Nucleic Acid Sensors

(2014) Surya Pandey et al.   Cold Spring Harbor Perspectives in Biology

Clearance of persistent HPV infection and cervical lesion by therapeutic DNA vaccine in CIN3 patients

(2014) Tae Jin Kim et al.   Nature Communications

The role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccination

(2014) Kristin Hollister et al.   Human Vaccines & Immunotherapeutics

Micro-minicircle Gene Therapy: Implications of Size on Fermentation, Complexation, Shearing Resistance, and Expression

(2014) Sofia Stenler et al.   Molecular Therapy-Nucleic Acids

Combined CpG and poly I:C stimulation of monocytes results in unique signaling activation not observed with the individual ligands

(2013) Ryan J. Arsenault et al.   CELLULAR SIGNALLING

Safety and Comparative Immunogenicity of an HIV-1 DNA Vaccine in Combination with Plasmid Interleukin 12 and Impact of Intramuscular Electroporation for Delivery

(2013) Spyros A. Kalams et al.   JOURNAL OF INFECTIOUS DISEASES

Oral vaccination with a liposome-encapsulated influenza DNA vaccine protects mice against respiratory challenge infection

(2013) Jing Liu et al.   JOURNAL OF MEDICAL VIROLOGY

In Vivo Electroporation of Minicircle DNA as a Novel Method of Vaccine Delivery To Enhance HIV-1-Specific Immune Responses

(2013) Q. Wang et al.   JOURNAL OF VIROLOGY

A Mini-intronic Plasmid (MIP): A Novel Robust Transgene Expression Vector In Vivo and In Vitro

(2013) Jiamiao Lu et al.   MOLECULAR THERAPY

Enhanced Nasal Mucosal Delivery and Immunogenicity of Anti-Caries DNA Vaccine through Incorporation of Anionic Liposomes in Chitosan/DNA Complexes

(2013) Liulin Chen et al.   PLoS One

Marker-free plasmids for biotechnological applications – implications and perspectives

(2013) Pedro H. Oliveira et al.   TRENDS IN BIOTECHNOLOGY

HIV/SIV DNA vaccine combined with protein in a co-immunization protocol elicits highest humoral responses to envelope in mice and macaques

(2013) Jinyao Li et al.   VACCINE

Antibiotic-free production of a herpes simplex virus 2 DNA vaccine in a high yield cGMP process

(2013) Jared Nelson et al.   Human Vaccines & Immunotherapeutics

A BCR/ABL-hIL-2 DNA Vaccine Enhances the Immune Responses in BALB/c Mice

(2013) Yanan Qin et al.   Biomed Research International

Synthetic DNA Vaccines: Improved Vaccine Potency by Electroporation and Co-Delivered Genetic Adjuvants

(2013) Seleeke Flingai et al.   Frontiers in Immunology

Influenza Virus H5 DNA Vaccination Is Immunogenic by Intramuscular and Intradermal Routes in Humans

(2012) J. E. Ledgerwood et al.   Clinical and Vaccine Immunology

Three types of humanCpG motifs differentially modulate and augment immunogenicity of nonviral and viral repliconDNA vaccines as built-in adjuvants

(2012) Yun-Zhou Yu et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

The Extragenic Spacer Length Between the 5′ and 3′ Ends of the Transgene Expression Cassette Affects Transgene Silencing From Plasmid-based Vectors

(2012) Jiamiao Lu et al.   MOLECULAR THERAPY

Minicircle DNA Vectors Achieve Sustained Expression Reflected by Active Chromatin and Transcriptional Level

(2012) Lia E. Gracey Maniar et al.   MOLECULAR THERAPY

Safety and Immunogenicity of an HIV-1 Gag DNA Vaccine with or without IL-12 and/or IL-15 Plasmid Cytokine Adjuvant in Healthy, HIV-1 Uninfected Adults

(2012) Spyros A. Kalams et al.   PLoS One

MDA5 Can Be Exploited as Efficacious Genetic Adjuvant for DNA Vaccination against Lethal H5N1 Influenza Virus Infection in Chickens

(2012) Matthias Liniger et al.   PLoS One

Immunotherapy Against HPV16/18 Generates Potent TH1 and Cytotoxic Cellular Immune Responses

(2012) M. L. Bagarazzi et al.   Science Translational Medicine

Sequential administration of cytokine genes to enhance cellular immune responses and CD4+T memory cells during DNA vaccination

(2012) Baowei Su et al.   Human Vaccines & Immunotherapeutics

A multiagent filovirus DNA vaccine delivered by intramuscular electroporation completely protects mice from ebola and Marburg virus challenge

(2012) Rebecca J. Grant-Klein et al.   Human Vaccines & Immunotherapeutics

Plasmid IL-12 electroporation in melanoma

(2012) Edward Cha et al.   Human Vaccines & Immunotherapeutics

A DNA Vaccine for Venezuelan Equine Encephalitis Virus Delivered by Intramuscular Electroporation Elicits High Levels of Neutralizing Antibodies in Multiple Animal Models and Provides Protective Immunity to Mice and Nonhuman Primates

(2011) Lesley C. Dupuy et al.   Clinical and Vaccine Immunology

Mutants of plant genes for developing cancer vaccines

(2011) Silvia Massa et al.   Human vaccines & immunotherapeutics

In Vivo Electroporation Enhances the Immunogenicity of an HIV-1 DNA Vaccine Candidate in Healthy Volunteers

(2011) Sandhya Vasan et al.   PLoS One

Incorporation of immunostimulatory motifs in the transcribed region of a plasmid DNA vaccine enhances Th1 immune responses and therapeutic effect against Mycobacterium tuberculosis in mice

(2011) Juan Wu et al.   VACCINE

Phase I study of a Neisseria meningitidis liposomal vaccine containing purified outer membrane proteins and detoxified lipooligosaccharide

(2011) Wendell D. Zollinger et al.   VACCINE

Unique Th1/Th2 Phenotypes Induced during Priming and Memory Phases by Use of Interleukin-12 (IL-12) or IL-28B Vaccine Adjuvants in Rhesus Macaques

(2010) M. P. Morrow et al.   Clinical and Vaccine Immunology

The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA

(2010) Peter Öhlschläger et al.   INTERNATIONAL JOURNAL OF CANCER

IL-15 trans-presentation by pulmonary dendritic cells promotes effector CD8 T cell survival during influenza virus infection

(2010) Jodi McGill et al.   JOURNAL OF EXPERIMENTAL MEDICINE

pFARs, Plasmids free of antibiotic resistance markers, display high-level transgene expression in muscle, skin and tumour cells

(2010) Corinne Marie et al.   JOURNAL OF GENE MEDICINE

Critical design criteria for minimal antibiotic-free plasmid vectors necessary to combine robust RNA Pol II and Pol III-mediated eukaryotic expression with high bacterial production yields

(2010) Aaron E. Carnes et al.   JOURNAL OF GENE MEDICINE

Essential Role for TLR9 in Prime but Not Prime-Boost Plasmid DNA Vaccination To Activate Dendritic Cells and Protect from Lethal Viral Infection

(2010) D. Rottembourg et al.   JOURNAL OF IMMUNOLOGY

Coexpressed RIG-I Agonist Enhances Humoral Immune Response to Influenza Virus DNA Vaccine

(2010) J. M. Luke et al.   JOURNAL OF VIROLOGY

A liposome-based platform, VacciMax®, and its modified water-free platform DepoVax™ enhance efficacy of in vivo nucleic acid delivery

(2010) Mohan Karkada et al.   VACCINE

Immunogenicity and protective efficacy of a DNA vaccine against Venezuelan equine encephalitis virus aerosol challenge in nonhuman primates

(2010) Lesley C. Dupuy et al.   VACCINE

Phase 1 clinical trials of the safety and immunogenicity of adjuvanted plasmid DNA vaccines encoding influenza A virus H5 hemagglutinin

(2010) Larry R. Smith et al.   VACCINE

The Toll-like receptor adaptor molecule TRIF enhances DNA vaccination against classical swine fever

(2010) Chao Wan et al.   VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice

(2009) Hui Hu et al.   BIOTECHNOLOGY LETTERS

DNA Vaccination with Electroporation Induces Increased Antibody Responses in Patients with Prostate Cancer

(2009) Lindsey Low et al.   HUMAN GENE THERAPY

KMP-11 DNA immunization significantly protects against L. donovani infection but requires exogenous IL-12 as an adjuvant for comparable protection against L. major

(2009) Suniti Bhaumik et al.   VACCINE

Vaxfectin® enhances both antibody and in vitro T cell responses to each component of a 5-gene Plasmodium falciparum plasmid DNA vaccine mixture administered at low doses

(2009) Martha Sedegah et al.   VACCINE

DNA vaccines: developing new strategies to enhance immune responses

(2008) Shaheed A. Abdulhaqq et al.   IMMUNOLOGIC RESEARCH

A Nasal Interleukin-12 DNA Vaccine Coexpressing Yersinia pestis F1-V Fusion Protein Confers Protection against Pneumonic Plague

(2008) H. Yamanaka et al.   INFECTION AND IMMUNITY

TANK-binding kinase-1 delineates innate and adaptive immune responses to DNA vaccines

(2008) Ken J. Ishii et al.   NATURE

Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime–protein boost HIV-1 vaccine in healthy human volunteers

(2008) Shixia Wang et al.   VACCINE

Combined effects of IL-12 and electroporation enhances the potency of DNA vaccination in macaques

(2008) Lauren A. Hirao et al.   VACCINE

Direct plasmid DNA encapsulation within PLGA nanospheres by single oil-in-water emulsion method

(2007) Hyejung Mok et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS