Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Antonio Casado-Diaz, Angel Rodriguez-Ramos, Barbara Torrecillas-Baena, Gabriel Dorado, Jose Manuel Quesada-Gomez, Maria Angeles Galvez-Moreno
Summary: Study found that phloretin can inhibit human adipogenesis by increasing beta-catenin and inducing apoptosis in adipocytes during late differentiation. This suggests the potential of phloretin-rich foods for clinical use and regenerative-medicine applications to combat obesity and prevent bone-marrow adiposity.
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Xinyun Xu, Ling Zhao, Paul D. Terry, Jiangang Chen
Summary: Mesenchymal stem cells from bone marrow can differentiate into either adipocytes or osteoblasts, and external stimuli such as environmental contaminants, heavy metals, dietary, and physical factors can influence this fate decision. Maintaining a balance between osteogenesis and adipogenesis is crucial for bone homeostasis, and disruptions in lineage commitment of these stem cells are associated with various bone disorders. This review focuses on understanding how external stimuli affect the fate of mesenchymal stem cells and calls for future studies to investigate the impact on bone health and underlying mechanisms of differentiation.
Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: For the use of bone marrow stromal cells (BMSC) in cartilage repair, controlling their hypertrophic differentiation is crucial. TGF-beta 1 stimulation triggers chondrogenic and hypertrophic differentiation in BMSCs, and silencing SP7, a transcription factor associated with hypertrophic differentiation, reduces both hypertrophic and chondrogenic differentiation processes. However, SP7 silencing compromises overall trilineage differentiation of BMSCs and makes it challenging to promote chondrogenesis while reducing hypertrophy in cartilage tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Review
Cell Biology
Chao Yang, Maowen Luo, Yu Chen, Min You, Qiang Chen
Summary: MicroRNAs play a critical role in regulating the differentiation of mesenchymal stem cells, providing clues for therapeutic strategies in regenerative medicine and biotherapy for related diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Haitao Wang, Peng Wei, Yi Zhang, Yuebai Li, Li Yin
Summary: lncRNA plays a significant role in osteoporosis, regulating osteogenic differentiation, adipogenic differentiation, and osteogenic-angiogenic coupling of bone marrow mesenchymal stem cells through the TCONS_00023297/miR-608/RUNX2/SHH signaling axis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Ziyi Li, Yafei Wang, Shilun Li, Yukun Li
Summary: Bone regeneration is a complex process involving the coordination of osteogenesis and osteoclastogenesis, with the balance between osteogenesis and adipogenesis of BMSCs playing a critical role. Recent studies have shown that exosomes from M2 macrophages facilitate osteogenesis and reduce adipogenesis through the miR-690/IRS-1/TAZ axis, potentially serving as a therapeutic tool for bone loss diseases.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Zheng-Zhao Liu, Chun-Gu Hong, Wen-Bao Hu, Meng-Lu Chen, Ran Duan, Hong-Ming Li, Tao Yue, Jia Cao, Zhen-Xing Wang, Chun-Yuan Chen, Xiong-Ke Hu, Ben Wu, Hao-Ming Liu, Yi-Juan Tan, Jiang-Hua Liu, Zhong-Wei Luo, Yan Zhang, Shan-Shan Rao, Ming-Jie Luo, Hao Yin, Yi-Yi Wang, Kun Xia, Lang Xu, Si-Yuan Tang, Rong-Gui Hu, Hui Xie
Summary: Senile osteoporosis (OP) is associated with decreased autophagic activity, with OPTN playing a crucial role in bone metabolism through selective autophagy. Reduced expression of OPTN during aging may lead to OP due to impaired degradation of FABP3 via selective autophagy, which impairs osteogenesis of MSCs and contributes to bone loss. Reactivating OPTN or inhibiting FABP3 could be a potential treatment strategy for senile OP.
Article
Biochemistry & Molecular Biology
Zun Wang, Xiaohua Li, Junxiao Yang, Yun Gong, Huixi Zhang, Xiang Qiu, Ying Liu, Cui Zhou, Yu Chen, Jonathan Greenbaum, Liang Cheng, Yihe Hu, Jie Xie, Xucheng Yang, Yusheng Li, Martin R. Schiller, Yiping Chen, Lijun Tan, Si-Yuan Tang, Hui Shen, Hong-Mei Xiao, Hong-Wen Deng
Summary: This study utilized single-cell RNA sequencing to identify distinct subpopulations of BM-MSCs in humans, shedding light on their cellular heterogeneity and roles in maintaining bone homeostasis, as well as potential novel markers for BM-MSC purification.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Genetics & Heredity
Ying Zhang, Ling Ling, Arya Ajay D. O. Ajayakumar, Yating Michelle Eio, Andre J. van Wijnen, Victor Nurcombe, Simon M. Cool
Summary: This study reveals a positive correlation between FGFR2 expression and osteogenic potential in human adult bone marrow-derived MSCs. Knockdown of FGFR2 leads to downregulation of pro-osteogenic genes and upregulation of pro-adipogenic genes, suppressing osteogenesis. Furthermore, FGFR2 knockdown results in upregulation of EZH2, an enzyme that regulates MSC lineage commitment and suppresses osteogenesis under osteogenic induction.
Article
Cell & Tissue Engineering
Hai-Bo Huang, Hai-Tao Luo, Na-Na Wei, Miao-Ling Liu, Fei He, Wei Yang, Jun Dong, Xiao-Fei Yang, Fu-Rong Li
Summary: This study revealed the landscape of lineage-specific circRNAs during adipo-osteogenesis of mesenchymal stem cells (MSCs) through integrative analysis. It identified circ-CRLF1 as a potential therapeutic target for skeleton-related diseases, providing new insights for their treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Huan Zhou, Lei Zhang, Yue Chen, Chun-Hui Zhu, Fa-Ming Chen, Ang Li
Summary: Bone formation is a complex regeneration process regulated by multiple signaling pathways, with the Hedgehog (Hh) pathway playing a crucial role. Dysregulation of the Hh pathway in adults is associated with bone-related diseases, while during repair processes, it can be reactivated to modulate bone formation. Current therapeutic strategies targeting the Hh cascade for osteogenesis coordination have been reviewed.
CELL PROLIFERATION
(2022)
Article
Cell & Tissue Engineering
Feng Zhi, Yi Ding, Rong Wang, Yujiao Yang, Kaiming Luo, Fei Hua
Summary: Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and regulates the balance between osteogenesis and adipogenesis in hBMSCs by sponging miR-431-5p.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Marine
Binbin Li
Summary: This study investigated the operability of walk-to-work operation through gangway for floating wind turbines and service operation vessels, focusing on the impact of second-order drift motion and hydrodynamic interactions. It was found that operational failure in the short wave peak period is primarily caused by horizontal displacement or velocity, while vertical motion dominates for longer periods.
Article
Engineering, Marine
Dongsheng Qiao, Peng Li, Gang Ma, Xuliang Qi, Jun Yan, Dezhi Ning, Binbin Li
Summary: A Long Short-Term Memory (LSTM) neural network model is developed to accurately predict mooring line responses based on vessel motion input, with a feature extraction method and judging standard proposed to improve prediction accuracy.
Article
Engineering, Marine
Dongsheng Qiao, Binbin Li, Jun Yan, Yu Qin, Haizhi Liang, Dezhi Ning
Summary: The study evaluated the performance changes of a FPSO with different modes of mooring line failure through time-domain numerical simulations. The results showed that the failure of mooring lines mainly influences the responses of sway, surge, and yaw, and the change rule is closely related to the stiffness and symmetry of the mooring system.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2021)
Review
Environmental Sciences
Dongsheng Qiao, Guangning Zhi, Haizhi Liang, Dezhi Ning, Jun Yan, Binbin Li
Summary: The passage discusses the role of physical model testing in the commercial utilization of wave energy converters, as well as the energy conversion principles, model scale requirements, and hydrodynamic similarity criteria involved. It proposes different similarity requirements for each stage of energy conversion process based on different mechanical factors and recommends consistent power scale ratios with Froude similarity for the three energy conversions.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Engineering, Ocean
Binbin Li
Summary: The hydrodynamic coupling between a semi-submersible floating wind turbine and an offshore support vessel during walk-to-work operation was studied, analyzing the complex multi-body hydrodynamic problem in the frequency domain. Three coupling strategies were proposed in the time domain simulation modeling to identify and evaluate coupling effects. The dynamic and mechanical properties of the wind turbine were validated, and it was found that neglecting hydrodynamic interaction led to overestimated motion of the support vessel and reduced motion of the wind turbine due to the 'shielding effect'.
APPLIED OCEAN RESEARCH
(2021)
Article
Engineering, Marine
Ziming Wang, Dongsheng Qiao, Jun Yan, Guoqiang Tang, Binbin Li, Dezhi Ning
Summary: This paper proposes a method to predict mooring line tension by using low-frequency and wave-frequency responses. By optimizing the neural network structure and using corresponding responses as input data, the low-frequency and wave-frequency tensions are predicted separately and then superposed to obtain the total tension. The feasibility is verified by considering different data relevance, and the method can significantly improve the accuracy of tension prediction.
Article
Engineering, Marine
Binbin Li, Dongsheng Qiao, Wenhua Zhao, Zhenzhong Hu, Sunwei Li
Summary: This study investigates the operability of using the new type of SWATH vessel for walk-to-work operations off the southeastern coast of China. The analysis of SWATH's motion behavior shows excellent operability for various transfer approaches and wave headings, although higher fuel consumption is observed under high sea-states. With an average operability greater than 95%, SWATH proves to be a more attractive vessel type compared to monohulls.
Article
Engineering, Marine
Zhengru Ren, Hongyu Zhou, Binbin Li, Zhenzhong Hu, Menghong Yu, Wei Shi
Summary: This paper introduces a novel localization scheme for moored floating structures, which utilizes tension measurements to provide real-time position references and estimate unknown anchor positions. The proposed method enhances safety by extracting redundant position information from tension measurements. A line-of-sight model and various considerations are presented to provide a realistic and robust tension-based localization scheme.
Article
Engineering, Marine
Li Yin, Dongsheng Qiao, Binbin Li, Haizhi Liang, Jun Yan, Guoqiang Tang, Jinping Ou
Summary: The parallel active motion compensated gangway is a new technology used in offshore wind service operation vessels to control large motion during the transfer process. This method controls the low frequency responses of the vessel using a DP system and compensates for the residual motion using an active parallel platform. The system identification method and inverse kinematics are used to achieve control and motion requirements.
Article
Engineering, Marine
Xinlong Wang, Dongsheng Qiao, Linxiao Jin, Jun Yan, Bin Wang, Binbin Li, Jinping Ou
Summary: This paper uses OpenFOAM to numerically simulate the wave run-up and wave load of the heaving cylinder by solving the Reynolds-averaged Navier-Stokes equation. The wave run-up of the heaving cylinder is decomposed into the relative run-up height and the absolute run-up height compared with the fixed cylinder, in which the phase difference between the absolute wave run-up curve and the cylindrical motion curve is the important factor. The different wave conditions are considered, including the short wave, resonance wave and long wave. The results show that the dimensionless maximum run-up height of the heaving cylinder changes nonlinearly with the incident wave frequency. In the range of about 23% of the resonance frequency of the heaving cylinder, the increase of the relative run-up height should be considered with the reason of resonance and phase differences. The dimensionless horizontal wave force increases nonlinearly with the increased scattering parameters. The fixed and heaving cylinder results differ significantly from the theoretical value when the ka value is larger than 0.25.
Article
Engineering, Marine
Wei Huang, Binbin Li, Xiaobo Chen
Summary: This study proposes a new quasi-dynamic method to assess the tendon mechanics and safety of the tension leg platform (TLP) system. By establishing an analytical tendon model and separating the hull's horizontal and vertical response as well as wave-frequency excitations, the method achieves simultaneous time history of tendon tension without the need for time-consuming convolution integral or finite element models. Validation through model test measurement and fully coupled analysis shows excellent agreement and demonstrates the reliability, accuracy, and efficiency of the method in TLP platform design.
Article
Engineering, Marine
Chenyu Wang, Jin Liu, Binbin Li, Wei Huang
Summary: Part I of this paper series introduces the absolute nodal coordinate formulation (ANCF), which is then applied in Part II to simulate flexible structures in offshore operation analysis. The in-house code MiNos, developed based on ANCF, is validated and used for static and dynamic time-domain analysis of flexible cables and mooring lines. Results are compared with commercial software OrcaFlex, demonstrating the accuracy of the in-house program and the potential of ANCF in dynamic analysis of marine operations. ANCF's high continuity and ability to mitigate numerical problems make it a promising theory for such analyses.
Article
Engineering, Marine
Binbin Li, Ziqi Liu, Hui Liang, Miaozi Zheng, Dongsheng Qiao
Summary: The hydrodynamic and seakeeping performances of a perforated fish farming vessel are studied using a numerical method that combines porous theory and potential flow theory. The effects of the openings on the motion response, wave load, and hydrodynamic coefficients are analyzed, and the results show that the modified vessel exhibits improved seakeeping performance, especially in terms of roll. The variation of the opening ratio significantly alters the natural frequencies and damping, and the hydrodynamic coefficients of heave increase with a lower natural frequency as the discharge coefficient and the number of openings increase. The wave loads exhibit a fluctuating behavior within a certain frequency range and become more pronounced with the effect of tank fluid increases. The spectral analysis results indicate a better performance of the perforated vessel in extreme sea states, making it feasible for offshore operations.
Article
Engineering, Marine
Wei Huang, Binbin Li, Do Kyun Kim
Summary: Synthetic fiber ropes are commonly used as the main material for towing lines, connecting towed ships and tug boats. The choice of different materials, such as polyester, aramid, and HMPE, can affect the behavior of the towing system and result in instability of the towed ship. In this study, a numerical model is proposed to consider the characteristics of fiber ropes in the towing system, particularly the dynamic stiffness, which is determined based on the mean load of the towing line. The effect of different types of fiber ropes and initial length on the performance of the towed ship under various wind speeds is thoroughly investigated. The results show that material characteristics significantly influence the directional stability of the ship, and the length of the towing line and wind strength can affect the fishtailing effect and motion period of the towed ship.
Article
Engineering, Marine
Binbin Li, Chenyu Wang
Summary: This paper series introduces the use of the absolute nodal coordinate formulation (ANCF) in modeling and studying flexible cables in various offshore floating operations. ANCF allows for arbitrary large deformation in flexible cables without considering centrifugal and Coriolis effects. The paper derives a linear expression for inertial forces and considers hydrodynamic-structure and soil-structure interactions in dynamic analysis. The in-house codes MiNos and KraKen are developed for dynamic cable analysis and multibody floating operation analysis, respectively.
