Article
Medicine, Research & Experimental
Mohammad Malekan, Mohammad Ali Ebrahimzadeh, Fateme Sheida
Summary: The adaptation to O2 loss is regulated by hypoxia-inducible factors such as HIF-1 alpha, which plays a crucial role in melanoma progression through various signaling pathways. Therefore, HIF-1 alpha serves as a diagnostic/prognostic biomarker and a potential target for therapy in melanoma.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Environmental Sciences
Qianqian Jiang, Qiannan Di, Dandan Shan, Qian Xu
Summary: Nonylphenol (NP), an endocrine disruptor, can induce apoptosis in Sertoli cells (SCs). This study found that exposure to NP inhibits lactate production and secretion in SCs, and disrupts intracellular homeostasis by reducing the expression of HIF-1α and inhibiting aerobic glycolysis, leading to ROS-mediated apoptosis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Medicine, Research & Experimental
Qi Liu, Chengcheng Guan, Cui Liu, Huayao Li, Jibiao Wu, Changgang Sun
Summary: This article discusses the expression and role of HIF-1 alpha in TNBC, including its regulation of various biological processes and its potential significance in immunotherapy, chemotherapy, anti-angiogenic therapy, and photodynamic therapy. The upstream regulatory factors and future directions of targeting HIF-1 alpha are also studied.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Oncology
Motoo Nagane, Koichi Ichimura, Ritsuko Onuki, Daichi Narushima, Mai Honda-Kitahara, Kaishi Satomi, Arata Tomiyama, Yasuhito Arai, Tatsuhiro Shibata, Yoshitaka Narita, Takeo Uzuka, Hideo Nakamura, Mitsutoshi Nakada, Yoshiki Arakawa, Takanori Ohnishi, Akitake Mukasa, Shota Tanaka, Toshihiko Wakabayashi, Tomokazu Aoki, Shigeki Aoki, Soichiro Shibui, Masao Matsutani, Keisuke Ishizawa, Hideaki Yokoo, Hiroyoshi Suzuki, Satoshi Morita, Mamoru Kato, Ryo Nishikawa
Summary: This study evaluated the efficacy and safety of bevacizumab in Japanese patients with newly diagnosed glioblastoma and identified an expression classifier that may predict patient prognosis.
Article
Oncology
Youliang Wu, Delong Meng, Yexiang You, Ruochuan Sun, Min Fu, Qiang Yan, Shangxin Zhang, Zheng Fang, Junjun Bao, Yongxiang Li
Summary: In gallbladder cancer (GBC), nuclear expression of HIF-1A is associated with higher tumor grade and worse survival, while in normal gallbladder tissues, the expression of cytoplasmic and nuclear HIF-1A is correlated with microvessel density.
Article
Multidisciplinary Sciences
Yukino Kobayashi, Ami Oguro, Yuta Hirata, Susumu Imaoka
Summary: The study reveals that PDI regulates HIF-1α by modulating its redox state for lysosome-dependent degradation, making it a novel factor in controlling HIF-1α.
Article
Chemistry, Medicinal
Claudia Quattrociocchi, Alberto Mangia, Silvio Aime, Valeria Menchise, Daniela Delli Castelli
Summary: The study focused on developing an MRI imaging probe that can target the hCAIX enzyme, which plays a significant role in the growth and survival of tumor cells. The results showed that the imaging probe exhibited high selectivity and sensitivity towards hCAI-expressing breast cancer cells. This approach holds great promise for the in vivo translation of a diagnostic procedure based on targeting hCA IX enzyme expression.
Article
Medical Laboratory Technology
Qing-Wei Lv, Zi-Qiang Zheng, Han Zhang, Mi Guo, Liang-Jun Shen
Summary: The study showed a strong association between serum HIF-1 alpha concentrations and trauma severity as well as poor 90-day outcomes in severe traumatic brain injury (sTBI) patients. Serum HIF-1 alpha concentrations above 280.2 pg/ml were identified as a promising prognostic biomarker for poor outcomes with high sensitivity and specificity.
CLINICA CHIMICA ACTA
(2021)
Article
Neurosciences
Bao-Bao Liang, Yu-Hong Wang, Jing-Jing Huang, Shuai Lin, Guo-Chao Mao, Zhang-Jian Zhou, Wan-Jun Yan, Chang-You Shan, Hui-Zi Wu, Amandine Etcheverry, Ya-Long He, Fang-Fang Liu, Hua-Feng Kang, An-An Yin, Shu-Qun Zhang
Summary: This study identified a set of CpGs that are associated with the efficacy of temozolomide (TMZ) treatment in glioblastoma (GBM) and constructed a risk signature that can predict overall survival of GBM patients treated with radiotherapy/TMZ. Functional studies indicated that one of the CpGs located in the gene GPR81 may have distinct impacts on TMZ resistance in GBM cells.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Anatomy & Morphology
Ester Parraga-Ros, Rafael Latorre-Reviriego, Monica Aparicio-Gonzalez, Talia Boronat-Belda, Octavio Lopez-Albors
Summary: This study characterized the immunolocalization of hypoxia markers (HIF-2α, GLUT1, and CAIX) in the porcine oviduct and found differential expression throughout the estrous cycle. The findings contribute to a better understanding of the cell physiology of the oviduct and could be useful in fertilization studies.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2023)
Article
Oncology
Vinodh Kannappan, Ying Liu, Zhipeng Wang, Karim Azar, Sathishkumar Kurusamy, Rajagopal S. Kilari, Angel L. Armesilla, Mark R. Morris, Mohammad Najlah, Peng Liu, Xiu-Wu Bian, Weiguang Wang
Summary: This study investigates the characteristics of Glioblastoma stem cells (GSC) under hypoxic conditions and examines the anti-GSC activity of a drug called disulfiram. The results show that disulfiram selectively kills GBM cells and inhibits the growth and migration of GSC without toxicity to vital organs.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Oncology
Yixin Yang, Hong Tang, Jiawen Zheng, Kai Yang
Summary: Current studies have shown that the decreased expression of PER1 gene is closely associated with the occurrence and progression of various malignant tumors, including oral squamous cell carcinoma (OSCC). This study reveals that PER1 promotes ferroptosis in OSCC cells by interacting with HIF-1 alpha. Furthermore, it is found that HIF-1 alpha inhibits PER1 transcription by binding to its promoter. Targeting the negative feedback loop between PER1 and HIF-1 alpha may provide a new strategy for OSCC treatment.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yukino Kobayashi, Ami Oguro, Susumu Imaoka
Summary: This study revealed that prolonged hypoxia increases cellular ROS levels, leading to the induction of HIF-1α regulators PHD2 and FIH-1. Cellular stress response enhances HIF-1α transcriptional activity by scavenging ROS, while Ref-1, regulated by ROS, plays a crucial role in HIF-1α activation and subsequent regulation of PHD2 and FIH-1.
FREE RADICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Julianne D. Twomey, Baolin Zhang
Summary: Circulating tumor cells (CTCs) are the source of metastasis and can be used as a liquid biopsy for monitoring cancer progression and treatment response. However, accurately detecting CTCs has been challenging due to their low frequency and heterogeneity. In this study, an in vitro model of CTCs was developed using non-adherent suspension culture to study breast cancer cell lines of different molecular subtypes. It was found that detached breast cancer cells accumulated cancer stem cells (CSCs) expressing stem cell markers and exhibiting high expressions of hypoxia-inducible gene products, especially CAIX. Inhibiting CAIX activity reduced CAIX expression and stem cell phenotypes in the targeted CTCs.
Article
Biotechnology & Applied Microbiology
Xiao-Yi Chen, Qian-Long Wang, Wei-Xue Huang, Long-Wei Li, Lan-Cong Liu, Yi Yang, You-Jiao Wu, Shan-Shan Song, Hao Ma, Hua Zhou, Pei Luo
Summary: This study aimed to analyze and evaluate the dynamic changes in HIF-1 alpha concentration in serum exosomes during bacterial peritonitis. A new colorimetric method to quantitate these changes was established. The results showed that the serum exosomal HIF-1 alpha levels increased in the early stage of bacterial peritonitis, reached a peak at 24-48 hours, and then gradually decreased. Intervention treatment reduced the abdominal infection extent, HIF-1 alpha concentration in serum exosomes, and the serum pro-inflammatory factors levels. These findings suggest that serum exosomal HIF-1 alpha can be used as a biomarker for predicting and monitoring the pathological process of bacterial peritonitis.
Article
Clinical Neurology
Anna Nigri, Stefania Ferraro, Claudia A. M. Gandini Wheeler-Kingshott, Michela Tosetti, Alberto Redolfi, Gianluigi Forloni, Egidio D'Angelo, Domenico Aquino, Laura Biagi, Paolo Bosco, Irene Carne, Silvia De Francesco, Greta Demichelis, Ruben Gianeri, Maria Marcella Lagana, Edoardo Micotti, Antonio Napolitano, Fulvia Palesi, Alice Pirastru, Giovanni Savini, Elisa Alberici, Carmelo Amato, Filippo Arrigoni, Francesca Baglio, Marco Bozzali, Antonella Castellano, Carlo Cavaliere, Valeria Elisa Contarino, Giulio Ferrazzi, Simona Gaudino, Silvia Marino, Vittorio Manzo, Luigi Pavone, Letterio S. Politi, Luca Roccatagliata, Elisa Rognone, Andrea Rossi, Caterina Tonon, Raffaele Lodi, Fabrizio Tagliavini, Maria Grazia Bruzzone
Summary: Neuroimaging studies often lack reproducibility. To address this issue, the RIN-Neuroimaging Network, a national consortium, was established to identify neuroimaging biomarkers of various neurological conditions. Through the establishment of standard procedures and a web-based infrastructure, this network aims to enhance data collection and sharing across multiple institutes.
FRONTIERS IN NEUROLOGY
(2022)
Article
Oncology
Laura Guarnaccia, Stefania E. Navone, Matteo M. Masseroli, Melissa Balsamo, Manuela Caroli, Silvia Valtorta, Rosa M. Moresco, Rolando Campanella, Luigi Schisano, Giorgio Fiore, Valentina Galiano, Emanuele Garzia, Giuseppe C. Appiani, Marco Locatelli, Laura Riboni, Giovanni Marfia
Summary: Glioblastoma is a highly aggressive primary brain tumor with limited treatment options. Recent studies have suggested that metformin, a commonly used antidiabetic drug, may have potential anticancer effects. In this study, the efficacy of metformin against glioblastoma endothelial cells (GECs) was tested. The results showed that metformin could significantly reduce cell viability and proliferation, induce apoptosis, and inhibit angiogenesis and invasiveness of GECs. Mechanistically, metformin was found to interfere with sphingolipid metabolism, shifting the balance towards the production of pro-apoptotic ceramide. These findings suggest that metformin may serve as an add-on therapy for glioblastoma treatment.
Article
Multidisciplinary Sciences
Rosalba Torrisi, Valentina Vaira, Laura Giordano, Annarita Destro, Vera Basilico, Saveria Mazzara, Piermario Salvini, Gabriella Gaudioso, Bethania Fernandes, Noemi Rudini, Giovanna Masci, Armando Santoro
Summary: This study retrospectively investigated the clinical, pathological, and molecular features of women with HR+/HER2 negative advanced breast cancer treated with fulvestrant. The results showed that the lack of visceral metastases, miRNA expression, and certain gene mutations were associated with 18-month progression-free survival.
SCIENTIFIC REPORTS
(2022)
Review
Hematology
Jurgen Thiele, Hans Michael Kvasnicka, Attilio Orazi, Umberto Gianelli, Naseema Gangat, Alessandro M. Vannucchi, Tiziano Barbui, Daniel A. Arber, Ayalew Tefferi
Summary: A group of international experts met to update the World Health Organization classification system for hematopoietic tumors and introduced the new International Consensus Classification (ICC) for Myeloid Neoplasms and Acute Leukemias. The focus of this review is on the ICC-2022 category of JAK2 mutation-prevalent myeloproliferative neoplasms (MPNs) and the importance of bone marrow morphology and genetic markers in disease classification and diagnostics.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Meeting Abstract
Hematology
Valentina Bellani, Daniele Cattaneo, Cristina Bucelli, Silvia Artuso, Umberto Gianelli, Francesco Onida, Alessandra Iurlo
Letter
Dermatology
Claudio Fenizia, Chiara Moltrasio, Luisa Ottobrini, Olga Utyro, Giovanni Genovese, Claudia Vanetti, Daria Trabattoni, Angelo V. Marzano
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Review
Pathology
Carlo Pescia, Gianluca Lopez, Umberto Gianelli, Giorgio Alberto Croci
Summary: Fibroblastic reticulum cell tumor (FRCT) is a rare dendritic neoplasm that usually occurs in lymph nodes but can also be found in the spleen and soft tissues. These tumors exhibit peculiar cytokeratin expression. The clinical presentation can range from very slow-growing to aggressive disease with features of malignancy. FRCT is challenging to diagnose due to its rarity and deceptive cytokeratin expression.
Article
Immunology
Ilaria Righi, Valentina Vaira, Letizia Corinna Morlacchi, Giorgio Alberto Croci, Valeria Rossetti, Francesco Blasi, Stefano Ferrero, Mario Nosotti, Lorenzo Rosso, Mario Clerici
Summary: This pilot study found that PD-1-expressing T lymphocytes were associated with acute rejection and the development of chronic lung allograft dysfunction (CLAD) in lung transplant patients, indicating a central role of PD-1 in the pathogenesis of rejection.
FRONTIERS IN IMMUNOLOGY
(2023)
Letter
Cardiac & Cardiovascular Systems
Saad Salman, Ludovica Lofino, Sara Mastinu, Angela Ammirabile, Marco Francone, Letterio Salvatore Politi, Ezio Lanza
CARDIOVASCULAR AND INTERVENTIONAL RADIOLOGY
(2023)
Article
Clinical Neurology
Umberto Pensato, Chiara de Philippis, Flavio Pistolese, Daniele Mannina, Simona Marcheselli, Letterio S. Politi, Armando Santoro, Stefania Bramanti
Summary: CAR T-cell therapy-related neurotoxicity is a cytokine-mediated neurological syndrome with diverse manifestations. This case study highlights a novel radiological finding of inflammatory foci in the corpus callosum, which can be reversed with high-dose methylprednisolone.
FRONTIERS IN NEUROLOGY
(2023)
Article
Endocrinology & Metabolism
Chiara Verdelli, Giulia Stefania Tavanti, Irene Forno, Valentina Vaira, Riccardo Maggiore, Leonardo Vicentini, Paolo Dalino Ciaramella, Francesca Perticone, Giovanni Lombardi, Sabrina Corbetta
Summary: This study found that osteocalcin (OC), a bone matrix protein, can modulate the function of parathyroid tumor cells by activating the calcium sensing receptor (CASR). OC reduces the activity of pERK/ERK and increases the levels of active beta-catenin through CASR activation. It also affects the expression of related genes to influence the physiological functions of parathyroid tumor cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Oncology
Annamaria Morotti, Francesco Gentile, Gianluca Lopez, Giulia Passignani, Luca Valenti, Marco Locatelli, Manuela Caroli, Claudia Fanizzi, Stefano Ferrero, Valentina Vaira
Summary: In this study, the differences in the epigenome of brain metastasis from colon cancer and lung cancer were analyzed and compared with primary tumors. The results show that colon tumors have a higher degree of genome-wide methylation compared to lung cancers. Brain metastasis from lung cancer deeply activates neural signatures, while colon brain metastases show activation of metabolic signaling. These signatures are specific for metastatic tumors and shed light on the epi/molecular mechanisms that colon and lung cancers adopt to thrive in the brain environment.
Review
Cell Biology
Leonie Saft, Hans M. Kvasnicka, Ludmila Boudova, Umberto Gianelli, Stefano Lazzi, Maria Rozman
Summary: Myeloid/lymphoid neoplasms with eosinophilia and tyrosine kinase gene fusions are rare hematopoietic tumors with diverse clinical and morphological presentations. Diagnosis of specific TK gene fusions may require additional evaluation with advanced molecular genetic techniques. The addition of new entities and genetic alterations involving TK genes in the latest classifications highlights the importance of studying M/LN-eo with TK gene fusions.
Article
Clinical Neurology
Delia Cannizzaro, Carla D. Anania, Mario De Robertis, Andrea Pizzi, Matteo Gionso, Chiara Ballabio, Maria Cleofe Ubezio, Gian Marco Frigerio, Massimiliano Battaglia, Emanuela Morenghi, Gabriele Capo, Davide Milani, Luca Attuati, Massimo Tomei, Marco Riva, Francesco Costa, Fabio Galbusera, Letterio S. Politi, Alessandro Ortolina, Maurizio Fornari
Summary: This study aimed to identify risk factors for adjacent-level syndrome after lumbar fusion surgery. Through a retrospective analysis of a large surgical series, the study identified age, BMI, preexisting disc degeneration, and postoperative pelvic tilt as the most relevant risk factors. In addition, patients older than 65 years experienced higher levels of clinical improvement and postsurgical satisfaction.
JOURNAL OF NEUROSURGERY-SPINE
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Riccardo Levi, Federico Garoli, Massimiliano Battaglia, Dario A. A. Rizzo, Maximilliano Mollura, Giovanni Savini, Marco Riva, Massimo Tomei, Alessandro Ortolina, Maurizio Fornari, Saurabh Rohatgi, Giovanni Angelotti, Victor Savevski, Gherardo Mazziotti, Riccardo Barbieri, Marco Grimaldi, Letterio S. Politi
Summary: This study aimed to evaluate the accuracy of machine learning in identifying physiological changes related to subjects' sex and age through analysis of radiomics features from lumbar vertebral CT images, and to determine its generalizability across different scanners. Spherical volumes-of-interest (VOIs) were annotated in the center of the vertebral body for each lumbar vertebra in 233 subjects who had undergone lumbar CT on 3 different scanners, and radiomics features from each VOI were evaluated. Machine learning models were used to identify subjects' sex and age, and a voting model was computed to combine predictions.
