Journal
ACS SYNTHETIC BIOLOGY
Volume 6, Issue 11, Pages 2021-2027Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00162
Keywords
chemically inducible dimerization; focused ultrasound; microbubble; mammalian cells
Categories
Funding
- Ministry of Science and Technology (MOST), Taiwan [MOST 105-2113-M-002-003, MOST 105-2221-E-007-055, MOST 105-2119-M-182-001, MOST 104-2311-B-007-001, MOST 105-2628-B-007-001-MY3]
- National Tsing Hua University
- Program for Translational Innovation of Biopharmaceutical Development-Technology Supporting Platform Axis-nMACS imaging
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We developed an ultrasound chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by at-short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.
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