Journal
TOXICOLOGY MECHANISMS AND METHODS
Volume 27, Issue 6, Pages 442-450Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/15376516.2017.1320458
Keywords
Antioxidant; colon epithelial cells; free radical; oxidative stress; virgin coconut oil polyphenol
Categories
Funding
- Kerala State Council for Science, Technology and Environment [01/FSHP-MAIN/2014/KSCSTE]
- Council of Scientific and Industrial Research [09/869(0012)/2012 EMR-I]
Ask authors/readers for more resources
Virgin coconut oil (VCO), extracted from the fresh coconut kernel, is a food supplement enriched with medium chain saturated fatty acids and polyphenolic antioxidants. It is reported to have several health benefits including lipid lowering, antioxidant and anti-inflammatory activities. The pharmacological benefits of VCO have been attributed to its polyphenol content (VCOP), the mechanistic basis of which is less explored. Liquid chromatography/mass spectroscopy (LC/MS) analysis of VCOP documented the presence of gallic acid, ferulic acid (FA), quercetin, methyl catechin, dihydrokaempferol and myricetin glycoside. Pre-treatment of VCOP at different concentrations (25-100 mu g/mL) significantly reduced the H2O2 and 2,2 '-azobis (2-amidinopropane) dihydrochloride (AAPH) induced cell death in HCT-15 cells. Giving further insight to its mechanistic basis, oxidative stress induced alterations in glutathione (GSH) levels and activities of GR (Glutathione-Reductase), GPx (Glutathione-Peroxidase), GST (Glutathione-S-Transferase) and catalase (CAT) were restored to near-normal by VCOP, concomitantly reducing lipid peroxidation. The efficacy of VCOP was similar to that of Trolox and FA added in culture. The study thus suggests that VCOP protects cells from pro-oxidant insults by modulating cellular antioxidant status.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available