Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Food Science & Technology
Jie Zhou, Teng-Yu Wang, Zhi Lan, Han-Jie Yang, Xing-Jian Ye, Rui Min, Zhao-Hui Wang, Qing Huang, Jing Cao, Yu-E Gao, Wen-Long Wang, Xiu-Lan Sun, Yi Zhang
Summary: Foodborne microbial contamination (FMC) is the primary cause of food poisoning and illness. Isothermal amplification methods with functional nucleic acids (FNAs) have enabled the detection of mycotoxins, making biosensors for FMC more sensitive, cost-effective, and less reliant on nanomaterials for signal output.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Xiuen Cao, Chuanpin Chen, Qubo Zhu
Summary: This review summarizes the applications of functional nucleic acids and isothermal amplification techniques in biosensing, highlighting their important contributions to the field. These technologies have great potential to enhance the performance of biosensors.
Article
Chemistry, Analytical
Rui Mao, Tianzuo Wang, Yue Zhao, Xinyao Wu, Shun Zhang, Ting Cai
Summary: The article presents a novel isothermal amplification method called closed dumbbell mediated isothermal amplification (CDA) for nucleic acids. The method offers the advantages of simple primer design, high amplification efficiency, and successful detection of virus genes and bacterial pathogens. Real-time and colorimetric CDA detection methods have also been developed and proven effective.
Article
Biochemistry & Molecular Biology
Xu Chen, Qingxue Zhou, Xueli Wu, Shuoshi Wang, Rui Liu, Shilei Dong, Wei Yuan
Summary: The study introduced a novel method, LAMP-PNB, for identifying N. gonorrhoeae in clinical samples, which could be completed within 1 hour, including DNA preparation, amplification reaction, and reporting.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Junyeong Lee, Hyowoong Noh, Chang-Ju Lee, Ju-Hyun Bae, Moon-Chang Baek, Muhan Choi, Sung-Wook Nam, Hyun-Hwa Cha, Gun Oh Chong, Hyung Soo Han, Hongsik Park
Summary: The diagnosis of pathogen infection using loop-mediated isothermal amplification (LAMP) is a fast, cost-effective, and highly specific method for detecting target DNAs. However, the subjective nature of naked-eye observation can significantly affect the diagnosis result. In this study, a colorimetric sensor platform was developed to accurately monitor the color change of LAMP solutions for diagnosing pathogen infection. The method showed high accuracy when tested with clinical samples for Streptococcus agalactiae bacterial infection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Daehan Nam, Seokjoon Kim, Jung Ho Kim, Seungjin Lee, Daneub Kim, Jinseo Son, Doyeon Kim, Byung Seok Cha, Eun Sung Lee, Ki Soo Park
Summary: Loop-mediated isothermal amplification (LAMP) is a widely used molecular diagnostics technology. This study introduces a new version of LAMP, called low-temperature LAMP, which operates at 37 degrees C for on-site diagnosis. The low-temperature LAMP differs from conventional LAMP in terms of MgSO4 and dNTPs concentrations, as well as the lengths of DNA probes. Under optimal conditions, low-temperature LAMP shows comparable amplification efficiency to conventional LAMP. Additionally, the method enables the detection of miR-21 with high sensitivity and selectivity at 37 degrees C, without requiring expensive DNA modifications or special additives.
Article
Multidisciplinary Sciences
Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada
Summary: This study developed a RT-LAMP technique for the diagnosis of HPeVs, particularly HPeV3, allowing for rapid and convenient detection of HPeV3 RNA. The technique showed clinical utility in diagnosing newborns and infants with sepsis-like symptoms.
Article
Chemistry, Analytical
Manpreet Kaur, Khadijah Ayarnah, Danielle Duanis-Assaf, Noam Alkan, Evgeni Eltzov
Summary: In this study, a paper-based device for reverse transcription loop-mediated isothermal amplification (RT-LAMP) was proposed for real-time simultaneous detection of C. gloeosporioides latent infections in tomatoes. The assay was found to be highly selective, with a detection limit of 0.5 pg of total extracted RNA. The results were generated within 40 minutes, making it suitable for identifying C. gloeosporioides in resource-limited settings.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Rui Mao, Xinyao Wu, Qing Miao, Ting Cai
Summary: Nucleic acid-based assays, such as Loop-mediated isothermal amplification (LAMP), have been widely used in clinical diagnostics, food safety, and environmental monitoring. A novel method called asymmetric stem-loop-mediated isothermal amplification (ASLAMP) was developed to overcome the challenges of specific primer design and non-specific amplification associated with LAMP. ASLAMP showed promising results in detecting the H1N1 gene fragment with simple primer design, short target sequence, and high amplification efficiency, offering potential for point-of-care DNA isothermal amplification.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Ane Rivas-Macho, Unai Eletxigerra, Ruth Diez-Ahedo, Angela Barros, Santos Merino, Felipe Goni-de-Cerio, Garbine Olabarria
Summary: An electrochemical sensor based on loop-mediated isothermal amplification (LAMP) technology was developed for the detection of SARS-CoV-2 virus in clinical samples. The sensor eliminates the need for DNA extraction by heating the sample, reducing the time required for each measurement.
Article
Chemistry, Analytical
Seokhwan Kim, Jung Ho Kim, Seokjoon Kim, Jung Soo Park, Byung Seok Cha, Eun Sung Lee, Jinjoo Han, Jiye Shin, Youngjun Jang, Ki Soo Park
Summary: A loop-mediated isothermal amplification-based nucleic acid lateral flow assay (LAMP-NALFA) system was developed for specific and multiplex detection of genetic markers at a low cost. The system demonstrated high selectivity in discriminating target amplification products and non-specific amplification products, and showed good practical applicability in different samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Guangcheng Luo, Tingting Yi, Qiang Wang, Bin Guo, Li Fang, Guoyuan Zhang, Xiaolan Guo
Summary: This study introduces a novel nucleic acid detection method called SPA, which amplifies target nucleic acids specifically and sensitively using Bst DNA polymerase and stem-loop primers. Compared to traditional PCR and LAMP technologies, SPA is more specific and convenient. The pH indicator-based colorimetric SPA showed 100% accuracy for HPV16 detection in cervical samples, indicating its great potential for POCT nucleic acid testing.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Public, Environmental & Occupational Health
Xiaolan Huang, Fei Xiao, Nan Jia, Chunrong Sun, Jin Fu, Zheng Xu, Xiaodai Cui, Hui Huang, Dong Qu, Juan Zhou, Yi Wang
Summary: The ongoing outbreak of monkeypox, caused by monkeypox virus (MPXV), requires a rapid and sensitive MPXV detection method. In this study, a diagnostic test called MPX-LAMP-LFB was developed based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the diagnosis of MPXV infection. The MPX-LAMP-LFB assay can specifically detect MPXV strains without cross-reaction with non-MPXV pathogens and has a low sensitivity of 5 copies/µl of plasmid template and 12.5 copies/µl of pseudovirus in human blood samples. The entire process of the assay can be completed in about 1 hour, making it a useful tool for rapid and reliable diagnosis of MPXV infection.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemical Research Methods
Mun-Bum Song, Hyou-Arm Joung, Young Kyoung Oh, Kwonyoung Jung, Young Deok Ahn, Min-Gon Kim
Article
Biochemical Research Methods
Cheng Guo Li, Hyou-Arm Joung, Hyungrye Noh, Mun-Bum Song, Min-Gon Kim, Hyungil Jung
Article
Chemistry, Analytical
Donggu Hong, Hyou-Arm Joung, Do Young Lee, Sanghyo Kim, Min-Gon Kim
SENSORS AND ACTUATORS B-CHEMICAL
(2015)
Article
Biophysics
Kahee Kim, Hyou-Arm Joung, Gyeo-Re Han, Min-Gon Kim
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Medicine, Research & Experimental
Jusung Oh, Hyou-Arm Joung, Hyung Soo Han, Jong Kun Kim, Min-Gon Kim
Article
Chemistry, Analytical
Md. Rajibul Akanda, Hyou-Arm Joung, Vellaiappillai Tamilavan, Seonhwa Park, Sinyoung Kim, Myung Ho Hyun, Min-Gon Kim, Haesik Yang
Article
Food Science & Technology
Hyun-Kyung Oh, Hyou-Arm Joung, Minhyuk Jung, Hohjai Lee, Min-Gon Kim
Article
Chemistry, Multidisciplinary
Hyou-Arm Joung, Zachary S. Ballard, Jing Wu, Derek K. Tseng, Hailemariam Teshome, Linghao Zhang, Elizabeth J. Horn, Paul M. Arnaboldi, Raymond J. Dattwyler, Omai B. Garner, Dino Di Carlo, Aydogan Ozcan
Article
Chemistry, Multidisciplinary
Artem Goncharov, Hyou-Arm Joung, Rajesh Ghosh, Gyeo-Re Han, Zachary S. Ballard, Quinn Maloney, Alexandra Bell, Chew Tin Zar Aung, Omai B. Garner, Dino Di Carlo, Aydogan Ozcan
Summary: This study demonstrates a point-of-care serodiagnosis assay that can simultaneously quantify three biomarkers of acute cardiac injury. Using a paper-based fluorescence vertical flow assay (fxVFA) processed by a low-cost mobile reader, the platform achieves fast testing time and high sensitivity for the target biomarkers. Validation using human serum samples shows minimal cross-reactivity and accurate quantification of the three biomarkers. Blind testing with individually activated cartridges further confirms the accuracy and reliability of this multiplexed computational fxVFA. The inexpensive paper-based design and handheld footprint make this platform a promising point-of-care sensor for diagnostics in resource-limited settings.
Article
Biochemical Research Methods
Yi Luo, Hyou-Arm Joung, Sarah Esparza, Jingyou Rao, Omai Garner, Aydogan Ozcan
Summary: The research presents a quantitative particle agglutination assay that combines mobile lens-free microscopy and deep learning to rapidly measure the concentration of a target analyte. Using 88 unique patient serum samples, the platform achieved good matches in the hs-CRP range (0-10 μg/mL) and successfully differentiated high CRP concentrations.
Article
Health Care Sciences & Services
Zachary S. Ballard, Hyou-Arm Joung, Artem Goncharov, Jesse Liang, Karina Nugroho, Dino Di Carlo, Omai B. Garner, Aydogan Ozcan
NPJ DIGITAL MEDICINE
(2020)
Article
Chemistry, Analytical
Aniruddha Ray, Sarah Esparza, Dimei Wu, Mark R. Hanudel, Hyou-Arm Joung, Barbara Gales, Derek Tseng, Isidro B. Salusky, Aydogan Ozcan
Article
Radiology, Nuclear Medicine & Medical Imaging
Youngung Seok, Hyungjun Jang, Jusung Oh, Hyou-Arm Joung, Min-Gon Kim
BIOMEDICAL PHYSICS & ENGINEERING EXPRESS
(2019)
Article
Biochemical Research Methods
Hyou-Arm Joung, Zachary S. Ballard, Alice Ma, Derek K. Tseng, Hailemariam Teshome, Spencer Burakowski, Omai B. Garner, Dino Di Carlo, Aydogan Ozcan