Journal
THERANOSTICS
Volume 7, Issue 7, Pages 2067-2077Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.19427
Keywords
Cardiomyocytes; hPSCs
Categories
Funding
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Korean government (MSIP) [2015M3A9C6031514]
- Korea Health Technology R&D Project through Korea Health Industry Development Institute (KHIDI)
- Ministry of Health & Welfare, Republic of Korea [HI15C2782, HI16C2211]
- NHLBI [R01HL127759, R01HL129511]
- NIDDK [DP3-DK108245]
- CityU Start-up Grant [7200492]
- CityU Research Project [9610355]
- Georgia Immuno Engineering Consortium through Georgia Institute of Technology, Emory University
- Georgia Research Alliance
- Korea Health Promotion Institute [HI16C2211010017] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) are considered a most promising option for cell-based cardiac repair. Hence, various protocols have been developed for differentiating hPSCs into CMs. Despite remarkable improvement in the generation of hPSC-CMs, without purification, these protocols can only generate mixed cell populations including undifferentiated hPSCs or non-CMs, which may elicit adverse outcomes. Therefore, one of the major challenges for clinical use of hPSC-CMs is the development of efficient isolation techniques that allow enrichment of hPSC-CMs. In this review, we will discuss diverse strategies that have been developed to enrich hPSC-CMs. We will describe major characteristics of individual hPSC-CM purification methods including their scientific principles, advantages, limitations, and needed improvements. Development of a comprehensive system which can enrich hPSC-CMs will be ultimately useful for cell therapy for diseased hearts, human cardiac disease modeling, cardiac toxicity screening, and cardiac tissue engineering.
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