Article
Plant Sciences
Lingling Fang, Amit Raj Sharma, Chineche Aniemena, Krystian Roedel, Florence Henry, Philippe Moussou, Anita Samuga, Fabricio Medina-Bolivar
Summary: Stilbene and benzofuran derivatives isolated from white mulberry root have shown various biological activities. Multiple elicitors, including CD, MgCl2, MeJA, and H2O2, were used to enhance the production of these bioactive compounds. The results showed that co-treatment with CD, H2O2, and MgCl2 significantly increased the accumulation of stilbenes and benzofurans in hairy root cultures.
Article
Agronomy
Shangqian Qin, Yanrong Liu, Jianping Yan, Shiwen Lin, Wanjun Zhang, Bingwu Wang
Summary: Rhizobium rhizogenes-mediated plant hairy root induction is a convenient method for studying root-specific gene function. In this study, we developed an optimized tobacco hairy root induction system using different R. rhizogenes strains and media. We found that the strain LBA9402 exhibited the highest induction rate and could also deliver foreign genes to hairy roots. Overexpression of the MsSPL12 gene significantly increased nicotine production in transgenic hairy roots. Our results suggest that this system could be used for efficient manipulation of tobacco nicotine biosynthesis.
Review
Plant Sciences
Kevin J. Morey, Christie A. M. Peebles
Summary: While plants are a valuable source of natural products, their commercial application faces challenges. Hairy root cultures provide a potential production platform for non-agricultural plants, with advancements in metabolic engineering, synthetic biology tools, and bioreactor technology enabling commercial application.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Ruth Hornedo-Ortega, Michael Jourdes, Gregory Da Costa, Arnaud Courtois, Julien Gabaston, Pierre-Louis Teissedre, Tristan Richard, Stephanie Krisa
Summary: Stilbene metabolites are of great interest due to their similar or even stronger biological effects compared to their parent compounds. Their study is highly relevant as they are predominant in biological fluids. The metabolism of oxyresveratrol (ORV) and gnetol (GN) resulted in the formation of several glucuronide metabolites, which showed the ability to decrease the production of inflammatory factors in stimulated macrophages.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Jiao Jiao, Lan Yao, Jin-Xian Fu, Yao Lu, Qing-Yan Gai, Xue Feng, Xiao-Jia He, Run-Ze Cao, Yu-Jie Fu
Summary: Pigeon pea hairy root cultures (PPHRCs) were cocultured with Aspergillus oryzae CGMCC 3.951 (AO 3.951) to enhance the production of cajaninstilbene acid (CSA), a health-promoting compound. AO 3.951 elicitation led to a significant increase in CSA content in both hairy roots and culture media, while reducing flavonoid glycosides. AO 3.951 also activated the expression of key enzyme genes involved in CSA biosynthesis and transporter genes associated with CSA transmembrane transport. The cocultivation approach holds great potential for commercial production of high-value phenolic compounds like CSA.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Seungeun Baek, Jong-Eun Han, Thanh-Tam Ho, So-Young Park
Summary: This study tested the efficiency of hairy root induction in Centella asiatica using leaf and petiole explants. The results showed that petiole-derived hairy roots had the highest growth index and triterpenoid concentration. The findings suggest that hairy root culture of C. asiatica can be scaled up for mass production of triterpenoids.
Review
Plant Sciences
Diptesh Biswas, Avijit Chakraborty, Swapna Mukherjee, Biswajit Ghosh
Summary: This study highlights the importance of hairy roots in the production of secondary metabolites in Solanaceous plants. It provides an overview of modern biotechnological techniques used to enhance the production of these metabolites in hairy root cultures.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Khwanlada Kobtrakul, Dolly Rani, Asma Binalee, Pattarapol Udomlarp, Tatiya Srichai, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: Cannabis sativa L. (hemp) is widely used for medicinal, food, and textile purposes. This study focused on the roots of hemp, which have received less attention than other parts. By using hairy root cultures and treating them with salicylic acid, the levels of triterpenoids friedelin and epifriedelanol in the roots were significantly increased. These findings suggest that hairy root cultures with optimized elicitation could be an alternative method to produce valuable triterpenoids.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Minsol Choi, Jiwon Yoon, So Hwi Yang, Jae Kwang Kim, Sang Un Park
Summary: In this study, the optimal conditions for the hairy root culture of Salvia plebeia were determined, and its growth and accumulation of phenolic compounds were evaluated. The results showed that hairy roots cultured under optimal conditions exhibited enhanced phenolic compound accumulation and antioxidant activity, making them potential sources of antioxidants.
Article
Biotechnology & Applied Microbiology
Lucas Gaston Sosa Alderete, Hebe Ronchi, Natalia M. Monjes, Elizabeth Agostini
Summary: This study aimed to investigate the relationship between plant circadian clock and xenobiotic metabolism, focusing on the expression and activity of PODs genes. The results showed that the PODs genes exhibited circadian oscillations, and phenol treatment did not alter their expression patterns. The total POD activity also displayed marked oscillations, particularly in phenol-treated roots, with the highest levels observed at ZT23.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniela P. Herrera, Andrea M. Chanique, Ascension Martinez-Marquez, Roque Bru-Martinez, Robert Kourist, Loreto P. Parra, Andreas Schuller
Summary: This study reports the engineering of a resveratrol OMT from Vitis vinifera with high catalytic efficiency in producing pterostilbene. Through point mutations and variant design, the native substrate selectivity of VvROMT was successfully altered, indicating its potential for the tailor-made production of stilbenes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agricultural Engineering
Ganesan Mahendran, Narsingh Verma, Manju Singh, Karuna Shanker, Suchitra Banerjee, Birendra Kumar, Laiq ur Rahman
Summary: This study aimed to design an efficient hairy root induction protocol for Swertia chirayita and investigate the effects of methyl jasmonate, sodium nitroprusside, and salicylic acid on the production of bioactive xanthones. The results showed that the highest root induction frequency and biomass were achieved with specific cell density, infection time, and acetosyringone concentration. Furthermore, the content of bioactive xanthones was significantly increased with the application of methyl jasmonate. Overall, the developed hairy root protocol of Swertia chirayita is cost-effective and suitable for large-scale industrial production of bioactive xanthones.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Biochemistry & Molecular Biology
Caili Li, Meizhen Wang
Summary: This review summarizes the current progress on medicinal plant hairy root culture for bioactive compounds production, including the stimulating effects of various biotic and abiotic elicitors on the accumulation of secondary metabolites, as well as the synergistic effects by combination of different elicitors or with other strategies. Additionally, the transgenic system has promising prospects to increase bioactive compounds content by introducing their biosynthetic or regulatory genes into medicinal plant hairy root, offering great potential to further increase secondary metabolites yield by the integration of manipulating pathway genes with elicitors and other strategies. Finally, advances on two valuable pharmaceuticals production in the hairy root cultures are illustrated in detail, along with the successful production of bioactive compounds by hairy root culture in bioreactors.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Hyeon Ji Yeo, Min Jae Kwon, Sang Yeon Han, Jae Cheol Jeong, Cha Young Kim, Sang Un Park, Chang Ha Park
Summary: Agastache rugosa, also known as Korean mint, is a perennial aromatic medicinal plant that contains various phenolic compounds with pharmacological and physiological activities, including antioxidant, anticancer, antiviral, antifungal, and antibacterial properties.
Article
Biotechnology & Applied Microbiology
Sailendra Singh, Pallavi Pandey, Md. Qussen Akhtar, Arvind Singh Negi, Suchitra Banerjee
Summary: Through metabolic engineering, successful synthesis of curcumin and its glucoside in Atropa belladonna hairy roots has been achieved, leading to improved production yields.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Food Science & Technology
Nurul Huda, Xiangdong Li, Tanzim Jahan, Yuqi He, Chaonan Guan, Kaixuan Zhang, Ainong Gao, Milen I. Georgiev, Meiliang Zhou
Summary: Coix is a minor cereal crop with diverse bioactive compounds and significant therapeutic effects, but there is insufficient attention on breeding and genomic studies of Coix.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Hui Zhao, Yuqi He, Kaixuan Zhang, Shijuan Li, Yong Chen, Ming He, Feng He, Bin Gao, Di Yang, Yu Fan, Xuemei Zhu, Mingli Yan, Nathalie Giglioli-Guivarc'h, Christophe Hano, Alisdair R. Fernie, Milen Georgiev, Dagmar Janovska, Vladimir Meglic, Meiliang Zhou
Summary: Crop domestication leads to changes in the metabolites of Tartary buckwheat, with several genes identified as responsible for the synthesis of important medicinal components and defense molecules such as salicylic acid. The lower expression of these genes is associated with a reduction in salicylic acid and other medicinal compounds.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Review
Plant Sciences
Edgar Perez-Matas, Diego Hidalgo-Martinez, Ainoa Escrich, Miguel Angel Alcalde, Elisabeth Moyano, Mercedes Bonfill, Javier Palazon
Summary: Paclitaxel and its derivatives are widely used chemotherapeutic agents for cancer treatment, but their production remains challenging due to scarcity. This review discusses the progress in improving taxane production through genetic engineering techniques, including the overexpression of limiting genes and transcription factors in Taxus spp. cells and transformed roots, as well as attempts to produce taxanes in heterologous organisms. However, the transfer of the complete PTX metabolic route has not been achieved yet, and taxane biosynthesis is still restricted to Taxus cells and some endophytic fungi.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Medicine, Research & Experimental
Martina S. Savova, Liliya Mihaylova, Daniel Tews, Martin Wabitsch, Milen I. Georgiev
Summary: Obesity is a growing disorder that damages patients' quality of life and increases healthcare costs. Developing safe and innovative prevention strategies and treatments for obesity is crucial. The pathophysiology of obesity is complex and involves multiple signaling pathways, including the PI3K/AKT pathway, which plays a critical role in metabolic homeostasis. Natural compounds have been shown to target the PI3K/AKT signaling pathway and have potential for managing obesity. This paper evaluates the druggability of PI3K as a target and suggests perspectives on using natural compounds to modulate the PI3K/AKT pathway for obesity management.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Kristiana M. Amirova, Petya A. Dimitrova, Milena N. Leseva, Ivanka K. Koycheva, Albena T. Dinkova-Kostova, Milen I. Georgiev
Summary: CDDO-Me regulates neutrophil function by reducing necrosis and cell apoptosis, decreasing CXCR4 expression, increasing Nrf2 levels, and improving histological score of joint damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Diego Hidalgo Martinez, Anastasios Melis
Summary: Recent research has shown that exogenous genes from plants, humans, and bacteria can be stably over-expressed in cyanobacteria by forming functional heterohexameric structures with the CpcA alpha-subunit of phycocyanin and the 28.9 kDa phycocyanin disc linker polypeptide CpcG1. The study suggests that cyanobacteria tolerate the presence of heterologous recombinant proteins when they are fused with essential cellular proteins, such as phycocyanin, allowing for substantial and stable accumulation.
METABOLIC ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Ainoa Escrich, Diego Hidalgo, Mercedes Bonfill, Javier Palazon, Raul Sanchez-Munoz, Elisabeth Moyano
Summary: Novel approaches to optimize the production of plant specialized metabolites are crucial to reach maximum productivity of plant biofactories. Plant polyploidization frequently enhances protein synthesis and thereby increases the biosynthesis of specialized metabolites. By treating immature embryos of Taxus baccata with colchicine, polyploid cell lines were successfully induced, which showed higher taxane pathway gene expression and 6.2-fold higher taxane production compared to diploid cells. This finding is a step forward towards developing a sustainable production system for the anticancer drug paclitaxel.
Article
Biochemistry & Molecular Biology
Edgar Perez-Matas, Pascual Garcia-Perez, Mercedes Bonfill, Luigi Lucini, Diego Hidalgo-Martinez, Javier Palazon
Summary: Elicited cell cultures of Taxus spp. are sustainable biotechnological production systems of the anticancer drug paclitaxel. This study investigates the impact of elicitation on the synthesis of other bioactive compounds and reveals the differential effects of elicitors on cell growth and phenolic biosynthesis in Taxus baccata cell suspensions. Untargeted metabolomics analysis identifies numerous phenolic compounds and their changes over time, indicating the potential of Taxus ssp. biofactories to supply taxanes and valuable phenolic antioxidants.
Article
Biodiversity Conservation
Teodora Ivanova, Andrey Marchev, Mihail Chervenkov, Yulia Bosseva, Milen Georgiev, Ekaterina Kozuharova, Dessislava Dimitrova
Summary: In response to global challenges, including climate change, there is a need to enhance food security, which has led to a renewed interest in wild foods and foraging practices. This study focuses on the tradition of consuming spring edible ruderal plants in rural regions of Bulgaria. Data was gathered through field studies and interviews, and a total of 65 edible ruderals from 22 plant families were identified. The utilization of these plants varied, with some used in cooked dishes and others consumed raw in salads.
Article
Biology
Blagovesta Boneva, Andrey Marchev, Kristiana Amirova, Petya Ganova, Milen Georgiev, Andrey Tchorbanov, Nikolina Mihaylova
Summary: The aim of this study was to investigate the potential therapeutic effect of saffron extract in osteoarthritis. The results showed that saffron significantly reduced osteoarthritis-related joint histopathological alterations and decreased serum TNF alpha levels. Flow-cytometry analysis also revealed a decrease in pro-inflammatory immune cell subtypes in the spleen. These findings suggest that saffron could be a potential therapeutic approach for osteoarthritis patients.
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biochemistry & Molecular Biology
Juan Carlos Vera-Urbina, Susana Selles-Marchart, Ascension Martinez-Marquez, Maria Jose Martinez-Esteso, Maria Angeles Pedreno, Jaime Morante-Carriel, Roque Bru-Martinez
Summary: This study presents the characterization and optimization of t-R production in grape cell cultures. Factors such as growth phase, elicitor concentration, biomass, order of addition, and cell conditions were investigated. The optimal conditions for t-R production were determined, and the results can guide the upscaling of the bioproduction system.
Article
Plant Sciences
Edgar Perez-Matas, Pascual Garcia-Perez, Begona Miras-Moreno, Luigi Lucini, Mercedes Bonfill, Javier Palazon, Diego Hidalgo-Martinez
Summary: This study investigates the effects of coronatine (COR) and salicylic acid (SA) elicitors on taxane-associated metabolism in Taxus baccata cell cultures. Untargeted metabolomics analysis reveals pleiotropic effects induced by COR and SA, leading to changes in cell growth, taxane content, and secondary metabolism. Metabolite annotation shows significant effects on N-containing compounds, phenylpropanoids, and terpenoids. The highest level of paclitaxel content is observed on day 8 under SA elicitation, negatively correlating with the biomarkers kauralexin A2 and taxusin. This study provides valuable insights into the intricate metabolic changes associated with paclitaxel production.
Article
Plant Sciences
Miguel Angel Alcalde, Javier Palazon, Mercedes Bonfill, Diego Hidalgo-Martinez
Summary: This study explored the effects of overexpressing the squalene synthase (SQS) gene and the transcription factor TSAR2 on various aspects of C. asiatica hairy root lines. The transformed lines showed noticeable alterations in morphological traits and content of centelloside, phytosterol, and squalene. The expression of T-DNA genes modulated the centelloside biosynthesis genes. These findings provide valuable information for the biotechnological application of C. asiatica hairy roots.
Review
Plant Sciences
Anastasios Melis, Diego Alberto Hidalgo Martinez, Nico Betterle
Summary: This review addresses selected aspects of cyanobacterial production and scale-up methods, with a focus on the challenge and promise of specialty chemicals and proteins. The importance of synthetic biology with these microorganisms is also emphasized.
PHOTOSYNTHESIS RESEARCH
(2023)