Article
Engineering, Electrical & Electronic
Miranda Parisi, Massimiliano Lucidi, Paolo Visca, Gabriella Cincotti
Summary: This article reviews various super-resolution microscopy techniques utilized by microbiologists to investigate bacterial cell morphology and functions. The achieved resolution is discussed, with a focus on fluorophore selection and other critical imaging requirements. Recent applications are described, which have provided novel insights into bacterial cellular structures. The pivotal role of super-resolution microscopy in addressing antimicrobial resistance is emphasized.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Spectroscopy
Hulya Yilmaz, Shyam S. Mohapatra, Mustafa Culha
Summary: The study compared SERS and SEIRAS for microbial identification, showing that SEIRAS provides rich molecular information and improved discrimination power compared to FTIR, making it a potential technique for microbial analysis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biophysics
Qiuyan Chen, Liyan Zhao, Hong Liu, Qianqian Ding, Chenghao Jia, Sihao Liao, Ningtao Cheng, Min Yue, Shikuan Yang
Summary: In this study, nanoporous Ag nanorods were prepared through chemical reduction of electrodeposited Ag7O8NO3 nanorods using a reducing agent. The nanopores in the Ag nanorods can function as both analyte containers and SERS hot spots, providing strong SERS enhancement. The simple fabrication process and outstanding SERS performance make the nanoporous Ag nanorods promising candidates for sensitive detection of various pollutants, narcotics, and biomolecules.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xiao-Tong Pan, Xuan-Ye Yang, Tian-Qi Mao, Kang Liu, Zao-Zao Chen, Li-Na Ji, De-Chen Jiang, Kang Wang, Zhong-Ze Gu, Xing-Hua Xia
Summary: In this study, we used silver nanowires to extract SERS spectra in situ from cell culture models and applied machine learning algorithm for analysis. The results showed significant differences in protein abundance between 2D and 3D models, and nucleic acid-rich and protein-rich regions can be accurately distinguished.
Review
Chemistry, Multidisciplinary
Wei Li, Gabriele S. Kaminski Schierle, Bingfu Lei, Yingliang Liu, Clemens F. Kaminski
Summary: Super-resolution imaging techniques have become popular for visualizing cellular structures with nanometric resolution. This review focuses on the use of fluorescent nanoparticles (NPs) as bright and photostable probes for super-resolution imaging. The review provides an overview of different super-resolution methods, discusses their demands on the properties of fluorescent NPs, and reviews the features, strengths, and weaknesses of each NP class. The future outlook and opportunities in material science for the development of probes for multiplexed subcellular imaging with nanometric resolution are also discussed.
Article
Biochemical Research Methods
Cristina-Cassiana Andrei, Anne Moraillon, Eric Larquet, Monica Potara, Simion Astilean, Endre Jakab, Julie Bouckaert, Lea Rosselle, Nadia Skandrani, Rabah Boukherroub, Francois Ozanam, Sabine Szunerits, Anne Chantal Gouget-Laemmel
Summary: SERS is ideal for providing bacterial-specific molecular fingerprints, but generating reproducible spectra in complex structures like bacteria remains challenging. The study shows that the environment of bacteria affects the origin of SERS spectra, with densely packed bacteria exhibiting characteristic signatures related to secretion or cell structures, and isolated bacteria showing spectral variabilities. The variabilities do not prevent discrimination of different E. coli strains based on SERS signatures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Wei Lin, Gary C. H. Mo, Sohum Mehta, Jin Zhang
Summary: Super-resolution activity imaging currently does not consider the locations of collaborating regulators/effectors in living cells. The DrFLINC technique, based on the FLINC principle, utilizes nonfluorescent Dronpa to enhance fluorescence fluctuations, leading to the development of a superior red label and a next-generation activity sensor for context-rich super-resolution biosensing.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Physics, Applied
Wangqiao Chen, Hanbo Jiang, Xun Huang
Summary: This work presents a super-resolution acoustic imaging method that can invert the distribution, strength, and structure of sources in three-dimensional space. By utilizing the nonlinear coupling process between a low-frequency sound field and a high-frequency plane wave, this method overcomes the resolution limit. The reconstructed results with different source strengths and frequencies demonstrate the effectiveness of the proposed method, particularly for low-frequency source imaging. This method enhances the super-resolution analysis capability of acoustic imaging tests and provides deeper physical insights into various propulsion systems in underwater and aerospace systems.
APPLIED PHYSICS LETTERS
(2022)
Article
Nanoscience & Nanotechnology
Tristan C. Paul, Guy M. Hagen, Anatoliy O. Pinchuk, Kelly L. McNear
Summary: Creating sensitive and reproducible SERS substrates is a challenge. This study found that using a 405 nm laser to deposit lines of silver nanoparticles from a silver nitrate and sodium citrate solution yielded the best enhancement and consistency.
Review
Chemistry, Analytical
Xiaoxiao Jiang, Lu Kong, Yu Ying, Qiongchan Gu, Jiangtao Lv, Zhigao Dai, Guangyuan Si
Summary: Super-resolution optical imaging has become a hot spot in promoting nanotechnology and biotechnology research by overcoming the diffraction limit. Graphene, with its unique properties, has emerged as a meritorious candidate in high-resolution imaging. This article summarizes the working principle of graphene-assisted imaging devices and reviews the recent advances in super-resolution optical imaging based on graphene for both near-field and far-field applications.
Article
Spectroscopy
L. S. Murcia-Correa, O. C. Usuriaga, Lucia Vieira, L. Raniero
Summary: In this study, a DVD-R@AgNPs SERS substrate was prepared using the CCPD technique for detecting herbicide GLP. Scanning Electron Microscopy and Energy-dispersive X-ray spectroscopy were used to characterize the substrate and surface chemical changes. The DVD-R@AgNPs SERS sensor is a low-cost substrate that can analyze trace amounts of pesticides, demonstrating high SERS sensitivities and various applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Engineering, Electrical & Electronic
Emma J. Reid, Lawrence F. Drummy, Charles A. Bouman, Gregery T. Buzzard
Summary: This paper presents a Multi-resolution Data Fusion algorithm that accurately interpolates low-resolution electron microscope data. The algorithm utilizes small amounts of unpaired high-resolution data to train a neural network denoiser and incorporates a Multi-Agent Consensus Equilibrium problem formulation to balance the denoiser with a forward model agent for fidelity to measured data.
IEEE TRANSACTIONS ON COMPUTATIONAL IMAGING
(2022)
Article
Chemistry, Analytical
C. Suarez, W. R. Premasiri, H. Ingraham, A. N. Brodeur, L. D. Ziegler
Summary: A simple water extraction and transfer procedure is found to produce reproducible and highly sensitive SERS spectra of dried bloodstains on Ag nanoparticle substrates. The water/Ag methodology allows the detection and identification of diluted bloodstains on Ag substrates, while avoiding potential DNA damage. However, this water-only procedure is not effective on Au SERS substrates, due to the difference in the lysis and denaturation effects of blood cells on Ag and Au nanoparticles, respectively. The use of acetic acid is required for obtaining SERS spectra of dried bloodstains on Au substrates.
Article
Chemistry, Multidisciplinary
Carlos Puente, Nayely Pineda Aguilar, Idalia Gomez, Israel Lopez
Summary: Surface-enhanced Raman spectroscopy (SERS) substrates have attracted great interest due to the need for substrates with high sensitivity and low fabrication costs. In this study, Ag nanoparticles were photoconverted using a simple and affordable red-green-blue light-emitting diode photoreactor. The dispersions obtained were transformed into a paste of nanoparticles and used to fabricate SERS substrates through a simple drop-casting process. The performance of these substrates was tested and showed a significant enhancement in Raman intensities and substrate sensitivity with an increase in the number of faces and vertices in the particle.
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)