Article
Cell Biology
Li Chen, Ying-Chun Ouyang, Jian Li, Jing-Yi Qiao, Lin -Jian Gu, Zhen-Bo Wang, Yi Hou, Heide Schatten, Qing-Yuan Sun
Summary: The study indicates that Septin 4 plays a crucial role in regulating the G2/M transition in mouse oocytes by controlling the accumulation of CCNB1. Depletion of Septin 4 leads to GV arrest and alters the expression level of CDC20, while there is no significant change in the expression level of CDH1.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Min Gao, Yanling Qiu, Tianqi Cao, Dungao Li, Jingwen Wang, Yiren Jiao, Zhiyun Chen, Junjiu Huang
Summary: In this study, the role of HtrA2 in mouse oocyte maturation was investigated. The findings indicate that HtrA2 is a key regulator of oocyte maturation and its deficiency with age contributes to reproduction failure in females.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Developmental Biology
Meng-Meng Shan, Yuan-Jing Zou, Zhen-Nan Pan, Hao-Lin Zhang, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: The study reveals an important role of the motor protein KIFC1 in mouse oocyte meiosis, where it is involved in spindle migration and actin dynamics. Depletion of KIFC1 leads to abnormal meiotic division, disrupted chromosome alignment, and disturbed endoplasmic reticulum distribution.
Article
Fisheries
Jiawei Zhang, Yonglin Su, Ruzhuo Zhong, Qingheng Wang, Yu Jiao, Chuangye Yang
Summary: This study conducted transcriptome analysis of oocytes in Sipunculus nudus to identify key genes and pathways involved in GVBD process, revealing that hormonal action, calcium regulation, phosphorylation, and spindle microtubule action may participate in the process of GVBD.
AQUACULTURE RESEARCH
(2021)
Article
Endocrinology & Metabolism
Dongmei Deng, Juan Xie, Yin Tian, Ling Zhu, Xuemei Liu, Junxia Liu, Guoning Huang, Jingyu Li
Summary: Acquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation. Two approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage or post-IVM at the metaphase II (MII) stage. This study found that oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Xin Jin, Kehan Wang, Lu Wang, Wenwen Liu, Chi Zhang, Yuexin Qiu, Wei Liu, Huiyu Zhang, Dong Zhang, Zhixia Yang, Tinghe Wu, Jing Li
Summary: The study reveals that induction of mitophagy in oocytes can activate the PRKN-mediated pathway and block meiosis at metaphase I stage, with the regulatory role of RAB7 in rescuing observed defects. In aged GV oocytes, increased PINK1 and PRKN levels, along with decreased RAB7, lead to defective mitophagosome formation and impaired oocyte quality. Treatment with the RAB7 activator ML098 shows promise in ameliorating age-related deterioration of oocyte quality.
Article
Cell Biology
Yuan-Jing Zou, Meng-Meng Shan, Hong-Hui Wang, Zhen-Nan Pan, Meng-Hao Pan, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: Our study investigated the roles of RAB14 during oocyte meiotic maturation, showing that depletion of RAB14 caused large polar bodies and defects in spindle migration. The regulation on actin-based spindle migration and Golgi apparatus distribution by RAB14 during mouse oocyte meiotic maturation was suggested by our results.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Di Wu
Summary: The germinal vesicle (GV) stage is a critical transition point in mammalian oocyte development that determines oocyte quality through active RNA degradation and transcriptome reprofiling. Oocyte RNA-seq has revealed differences between non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) phases. Single oocyte RNA-seq (soRNA-seq) shows higher reproducibility and a better illustration of gene changes during oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiao-Han Li, Jia-Qian Ju, Zhen-Nan Pan, Hong-Hui Wang, Xiang Wan, Meng-Hao Pan, Yao Xu, Ming-Hong Sun, Shao-Chen Sun
Summary: The study demonstrates the critical role of PRC1 in midzone/midbody formation and cytokinesis during meiosis in mouse oocytes, with its regulation by KIF4A.
Article
Cell Biology
Daniela R. Chavez, Pei-Chih Lee, Pierre Comizzoli
Summary: The study identified BRD2 and ASRGL1 as critical factors for oocyte meiotic competence, while NPM1 did not show significant involvement in the process. Inhibition of BRD2 and NPM1 resulted in oocytes being unable to mature to the metaphase II stage, indicating their importance in oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Najmeh Davoodian, Ali Kadivar, Ebrahim Ahmadi, Hassan Nazari, Hossein Mehrban
Summary: The addition of Quercetin was found to have positive effects on the survival rate, cleavage rate, and total blastocyst rate of oocytes. It also protected vitrified oocytes against early apoptotic damage and improved their maturation and developmental competence after IVM.
Article
Cell Biology
Edgar del Llano, Rajan Iyyappan, Daria Aleshkina, Tomas Masek, Michal Dvoran, Zongliang Jiang, Martin Pospisek, Michal Kubelka, Andrej Susor
Summary: In mammalian females, the maturation and development of oocytes are regulated by various factors, including the maturation-promoting factor (MPF) complex and serum-glucocorticoid kinase proteins (SGK1). This study demonstrates that SGK1 is expressed in mouse oocytes and its activation promotes CDK1 activation, leading to the development and maturation of oocytes.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Yue Xue, Tie-Gang Meng, Ying-Chun Ouyang, Sai-Li Liu, Jia-Ni Guo, Zhen-Bo Wang, Heide Schatten, Chun-Ying Song, Xing-Ping Guo, Qing-Yuan Sun
Summary: This study found that Miro1 plays a crucial role in the maturation process of mouse oocytes by regulating mitochondrial homeostasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Guangyi Cao, Ruirui Guo, Manqi Chen, Chuanbo Sun, Jichang Wang
Summary: Poly(ADP-ribosyl)ation (PARylation), a vital post-translational modification, is involved in various biological processes. The mono(ADP-ribosyl) transferase PARP12 plays a crucial role in oocyte meiotic maturation in mice. Its depletion leads to abnormal spindle organization, chromosome misalignment, and activation of the spindle assembly checkpoint. Additionally, PARP12 depletion disrupts transcriptome homeostasis and affects the asymmetric division process.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Reproductive Biology
Qian Zhou, Ke Xu, Bing-Wang Zhao, Jing-Yi Qiao, Yuan-Yuan Li, Wen-Long Lei, Jian Li, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: Knockdown of MARCH5 in mouse oocytes leads to the arrest of oocyte maturation at the MI stage, characterized by the continuous activation of the spindle assembly checkpoint (SAC) and the failure of Cyclin B1 degradation. This may be due to the induction of mitochondrial dysfunctions by MARCH5 knockdown. MARCH5, a mitochondrial ubiquitin ligase that promotes mitochondrial elongation, is involved in regulating mitochondrial function during mouse oocyte meiotic maturation.
BIOLOGY OF REPRODUCTION
(2023)