Article
Immunology
Jonathan D. Cogen, Frankline M. Onchiri, Nicole Mayer Hamblett, Ronald L. Gibson, Wayne J. Morgan, Margaret Rosenfeld
Summary: The study showed that prolonged use of oral antibiotics in children with cystic fibrosis increased the risk of acquiring Achromobacter xylosoxidans, while treatment with intravenous antibiotics was associated with an increased risk of acquiring multidrug-resistant Pseudomonas aeruginosa and MRSA.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Infectious Diseases
Emma Reece, Pedro H. de Almeida Bettio, Julie Renwick
Summary: Pseudomonas aeruginosa is a dominant pathogen in cystic fibrosis airway disease, leading to significant inflammation, airway damage, and poor outcomes. Chronic infections of P. aeruginosa in CF patients are difficult to eradicate and are associated with worse lung function, higher morbidity, and reduced life expectancy. The complex community of microorganisms in the CF airway and their interactions may influence antimicrobial resistance and disease progression of P. aeruginosa.
Article
Immunology
Kelly Kweng, Andrea Benedetti, Yvonne Yau, Valerie Waters, Dao Nguyen
Summary: This study found that PA isolates from new-onset CF infections exhibit strain-specific susceptibility to neutrophil antibacterial functions, and PA isolates resistant to neutrophil phagocytosis are independent risk factors for failed tobramycin eradication. Experimental results on neutrophil phagocytosis and intracellular killing showed that isolates from persistent infections were more resistant compared to those from eradicated infections.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Marco Artini, Gianluca Vrenna, Marika Trecca, Vanessa Tuccio Guarna Assanti, Ersilia Vita Fiscarelli, Rosanna Papa, Laura Selan
Summary: Pseudomonas aeruginosa is a bacterium frequently involved in cystic fibrosis airway infections. It increases its virulence through biofilm, motility, toxin production, and invasion of host cells. The metalloprotease serratiopeptidase (SPEP) has been shown to impair the virulence-related properties of Gram-positive bacteria. In this study, the effects of SPEP on different physiological aspects related to the virulence of P. aeruginosa isolated from CF patients were investigated. The results showed that SPEP impaired attachment to inert surfaces and adhesion/invasion of eukaryotic cells. It also had strain-dependent effects on pyocyanin and pyoverdine production. Additionally, SPEP seemed to increase swarming motility and staphylolytic protease production. Further studies are needed to understand why different strains react differently to specific treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Parisa Bonyadi, Narjes Tehrani Saleh, Mohadeseh Dehghani, Mahsa Yamini, Kumarss Amini
Summary: This study aimed to evaluate the pattern of antibiotic resistance of Pseudomonas aeruginosa strains from patients with cystic fibrosis (CF) using a systematic review and meta-analysis. High resistance to most of the studied antibiotics was observed, indicating the need for antibiotic usage monitoring. Colistin was found to be the most appropriate treatment choice, but further randomized controlled trials are recommended.
MICROBIAL PATHOGENESIS
(2022)
Article
Biochemistry & Molecular Biology
Rafael Amieva, Teresa Gil-Gil, Jose Luis Martinez, Manuel Alcalde-Rico
Summary: Multidrug efflux pumps are crucial for antibiotic resistance in bacteria, but they also modulate other aspects of bacterial physiology, such as quorum sensing and iron homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Lise Goltermann, Kasper Langebjerg Andersen, Helle Krogh Johansen, Soren Molin, Ruggero La Rosa
Summary: A new mechanism of macrolide resistance caused by ribosomal protein mutations in Pseudomonas aeruginosa was investigated in this study. The results showed that macrolide antibiotics are still effective against P. aeruginosa and resistance development should be considered in patients receiving prolonged courses of macrolide treatment. Improved macrolide susceptibility testing is necessary for the detection of resistant bacteria.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Ijeoma N. Okoliegbe, Karolin Hijazi, Kim Cooper, Corinne Ironside, Ian M. Gould
Summary: This study compared the in vitro activity of new antimicrobials with other antipseudomonal agents and found colistin to be one of the most active antimicrobials, while combinations with beta-lactam plus beta-lactamase inhibitors showed synergistic effects. The study also highlights the importance of effective fluoroquinolone stewardship for CF patients.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Chemistry, Analytical
Zahra Rafiee, Seokheun Choi
Summary: A reliable and rapid AST device was developed to monitor bacterial growth and treatment efficacy in biofilm-based infections. The device measures changes in electrical outputs to assess the effectiveness of antibiotic treatment against pathogenic biofilms.
Article
Respiratory System
Jonathan D. Cogen, Anna Faino, Frankline Onchiri, Ronald L. Gibson, Lucas R. Hoffman, David P. Nichols, Margaret Rosenfeld, Matthew P. Kronman
Summary: The selection of antibiotics for managing pulmonary exacerbation in children with cystic fibrosis is usually based on previous respiratory culture results. However, there is currently a lack of data to guide the selection of antibiotics for children who have been culture negative for Pseudomonas aeruginosa (Pa) for at least one year.
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2022)
Article
Microbiology
Ronan A. Murphy, Matthew Coates, Sophia Thrane, Akshay Sabnis, James Harrison, Silke Schelenz, Andrew M. Edwards, Thomas Vorup-Jensen, Jane C. Davies
Summary: Pseudomonas aeruginosa is a common pathogen causing lung infections in people with cystic fibrosis. Glatiramer acetate, a drug used in multiple sclerosis treatment, has shown potential antipseudomonal activity. This study demonstrates that glatiramer acetate improves the efficacy of tobramycin, an antibiotic, against P. aeruginosa. It disrupts the bacterial cell wall and enhances the permeability of P. aeruginosa, allowing tobramycin to work more effectively. This research suggests that glatiramer acetate could be repurposed as an antibiotic resistance breaker for P. aeruginosa.
MICROBIOLOGY SPECTRUM
(2022)
Review
Microbiology
Christina S. Thornton, Michael G. Surette
Summary: Cystic fibrosis is the most common and lethal genetic disease among the Caucasian population, leading to chronic airway inflammation and declining pulmonary function. Studies have shown a diverse community of anaerobic bacteria in CF patients' airways, potentially impacting disease progression through synergistic interaction with principal pathogens. Despite the understanding of this complex bacterial milieu, the specific roles of anaerobes in disease progression remain unclear.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Xuerui Bao, Mona Bove, Tom Coenye
Summary: The high tolerance of Pseudomonas aeruginosa biofilms in respiratory tract infections in cystic fibrosis contributes to the failure of antibiotic therapy. This study identified carbon sources that could enhance the inhibiting and/or eradicating activity of tobramycin, ciprofloxacin, and ceftazidime against P. aeruginosa PAO1 biofilms. The mechanisms underlying the enhanced biofilm eradicating activity were strain-dependent.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Immunology
Veronica Roxana Flores-Vega, Silvia Yalid Vargas-Roldan, Jose Luis Lezana-Fernandez, Ricardo Lascurain, Jose Ignacio Santos-Preciado, Roberto Rosales-Reyes
Summary: Cystic fibrosis is a genetic disease caused by a mutation in the cftr gene, affecting chloride ion and water transport and leading to bacterial infections in the lungs. Autophagy plays a crucial role in pathogen clearance, but P. aeruginosa and B. cenocepacia have strategies to evade this pathway, resulting in chronic inflammatory immune responses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Cristina Cigana, Ruggero Giannella, Alice Colavolpe, Beatriz Alcala-Franco, Giulia Mancini, Francesca Colombi, Chiara Bigogno, Ulla Bastrup, Giovanni Bertoni, Alessandra Bragonzi
Summary: CFTR modulators have direct antimicrobial properties and can enhance antibiotic activity against bacteria in cystic fibrosis patients. Bacterial infections can also impact the levels of CFTR modulators in the airways. Optimizing treatment regimens based on the interaction between CFTR modulators and bacterial infections may improve personalized treatment efficacy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Anandi Martin, Michael J. Bland, Hector Rodriguez-Villalobos, Jean-Luc Gala, Philippe Gabant
Summary: This study assessed the potential of bacteriocins and their synergistic effects with anti-tuberculosis drugs against Mycobacterium tuberculosis. The researchers found bacteriocins with interesting antimycobacterial activity, and these bacteriocins showed significant synergistic effects when used in combination with rifampicin, ofloxacin, and moxifloxacin against susceptible and resistant clinical strains.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Engineering, Manufacturing
Farah Fahma, Afrinal Firmanda, Jaydee Cabral, Daniel Pletzer, John Fisher, Bhushan Mahadik, I. Wayan Arnata, Dewi Sartika, Anting Wulandari
Summary: This article reviews the use of 3D printed cellulose as an ideal wound dressing and their properties, including mechanical properties, permeability aspect, absorption ability, ability to retain and provide moisture, biodegradation, antimicrobial property, and biocompatibility. The applications of 3D printed cellulose in the management of chronic wounds, burns, and painful wounds are also discussed.
3D PRINTING AND ADDITIVE MANUFACTURING
(2023)
Article
Biochemistry & Molecular Biology
Igor V. Bodrenko, Tsedenia Alemu Zewdie, Jiajun Wang, Eshita Paul, Susanne Witt, Mathias Winterhalter
Summary: This study investigates protein-protein interaction using electrophysiology, focusing on the structure of the TolC-AcrA-AcrB complex and the inhibitory effect of potential efflux pump inhibitors. The findings suggest that the fluctuation of ion current and the average current through the TolC channel can be used as complementary measures to assess the interaction of the complex.
Article
Ecology
Christina L. Wiesmann, Yue Zhang, Morgan Alford, Corri D. Hamilton, Manisha Dosanjh, David Thoms, Melanie Dostert, Andrew Wilson, Daniel Pletzer, Robert E. W. Hancock, Cara H. Haney
Summary: Members of the bacterial genus Pseudomonas can form mutualistic, commensal, and pathogenic associations with diverse hosts. The study found that the regulatory gene ColR/S is functionally conserved between Pseudomonas aeruginosa and Pseudomonas fluorescens, but there are differences in the regulon. ColR/S allows Pseudomonas to sense and respond to a host, and the divergence of the ColR regulon may be related to different lifestyles.
Letter
Immunology
Kamilia Chahi, Christine Collienne, Ahalieyah Anantharajah, Hector Rodriguez-Villalobos, Philippe Hantson
EMERGING MICROBES & INFECTIONS
(2023)
Correction
Microbiology
Albert Ruiz-Sorribas, Herve Poilvache, Nur Hidayatul Nazirah Kamarudin, Annabel Braem, Francoise Van Bambeke
MICROBIOLOGY SPECTRUM
(2023)
Review
Mycology
Isidore W. Yerbanga, Seydou Nakanabo Diallo, Toussaint Rouamba, Olivier Denis, Hector Rodriguez-Villalobos, Isabel Montesinos, Sanata Bamba
Summary: This systematic review provides evidence of the existence of invasive aspergillosis (IA) in Africa, with a prevalence of up to 27% and a fatality rate of over 60%. The most common clinical form is invasive pulmonary aspergillosis, and predisposing conditions include neutropenia, HIV/AIDS, renal transplant recipients, and renal failure. The main management strategy is to initiate antifungal therapy after a failure of broad-spectrum antibiotic therapy.
JOURNAL DE MYCOLOGIE MEDICALE
(2023)
Article
Chemistry, Multidisciplinary
Yao Chen, Andrea Barba-Bon, Bohumir Gruner, Mathias Winterhalter, M. Alphan Aksoyoglu, Sushil Pangeni, Maryam Ashjari, Klaudia Brix, Giulia Salluce, Yeray Folgar-Camean, Javier Montenegro, Werner M. Nau
Summary: Cobalt bisdicarbollides (COSANs) are used as selective and efficient molecular carriers to transport hydrophilic oligopeptides through membranes. They can permeate lipid bilayer membranes without causing membrane damage. COSANs transport arginine-rich and lysine-rich peptides effectively, but cannot transport small molecules or anionic cargos. The transportation mechanism is a molecular carrier mechanism, and living cell experiments show that a fluorescent peptide cargo can be delivered into the cytosol.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Public, Environmental & Occupational Health
Daniel Valia, Juste Stephane Kouanda, Brecht Ingelbeen, Karim Derra, Berenger Kabore, Francois Kiemde, Toussaint Rouamba, Eli Rouamba, Franck Sovi Hien, Linda Campbell, Marie Meudec, Annie Robert, Halidou Tinto, Marianne A. B. van Der Sande, Hector Rodriguez Villalobos
Summary: Self-medication is common in rural Burkina Faso due to limited healthcare resources and easy access to antibiotics. This study emphasizes the importance of improving access to healthcare facilities and implementing antibiotic stewardship programs.
TROPICAL MEDICINE & INTERNATIONAL HEALTH
(2023)
Article
Microbiology
Juan Calvet-Seral, Estefania Crespo-Yuste, Vanessa Mathys, Hector Rodriguez-Villalobos, Pieter-Jan Ceyssens, Anandi Martin, Jesus Gonzalo-Asensio
Summary: Antimicrobial resistance (AMR) poses a threat to the prevention and treatment of infections caused by microorganisms. In this study, a method was developed to accurately determine the genotype-phenotype associations for putative antibiotic-resistant polymorphisms in Mycobacterium abscessus.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Infectious Diseases
Francoise Van Bambeke, Sebastian Wicha, Paul M. Tulkens, Markus Zeitlinger
Article
Multidisciplinary Sciences
Imane Saad Albichr, Samy Mzougui, Arnaud Devresse, Helene Georgery, Eric Goffin, Nada Kanaan, Jean Cyr Yombi, Leila Belkhir, Julien De Greef, Anais Scohy, Hector Rodriguez-Villalobos, Benoit Kabamba-Mukadi
Summary: The study evaluated the Quan-T-Cell SARS-CoV-2 test for measuring cellular immune responses in vaccinated healthy and immuno-suppressed subjects. The results showed that the test had good sensitivity and specificity for detecting specific T-cell responses against the SARS-CoV-2 spike protein, providing an additional tool for better management of COVID-19.
Review
Mycology
Isidore W. Yerbanga, Seydou Nakanabo Diallo, Toussaint Rouamba, Agustin Resendiz-Sharpe, Katrien Lagrou, Olivier Denis, Hector Rodriguez-Villalobos, Isabel Montesinos, Sanata Bamba
Summary: This review summarizes the available data on the performance of the disk diffusion method in determining triazole susceptibility profile of Aspergillus species. The results show that the disk diffusion method could be a suitable alternative to the reference methods for antifungal susceptibility testing in routine clinical microbiology laboratories.
JOURNAL DE MYCOLOGIE MEDICALE
(2023)
Article
Chemistry, Physical
Stefan Milenkovic, Jiajun Wang, Silvia Acosta-Gutierrez, Mathias Winterhalter, Matteo Ceccarelli, Igor V. Bodrenko
Summary: Transmembrane beta-barrel proteins are versatile candidates for various technological applications and their behavior is influenced by environmental conditions. Comparing two highly homologous porins, OmpF and OmpC, we observed subtle amino acid substitutions that can modulate mass transport properties. These differences can be attributed to the disparities in the environmental conditions under which the porins are expressed. Our analysis not only highlights the advantages of enhanced sampling methods, but also provides valuable insights into the biological function and technical applications of these proteins.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Microbiology
Xiaoya Wei, Jiacong Gao, Congjuan Xu, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Iain L. Lamont, Daniel Pletzer, Weihui Wu
Summary: This study reveals the response mechanism of P. aeruginosa to murepavadin and provides a promising antibiotic combination for the treatment of P. aeruginosa infections.
MICROBIOLOGY SPECTRUM
(2023)