Review
Biotechnology & Applied Microbiology
Tarana Siddika, Ilka U. Heinemann
Summary: MiRNAs are small non-coding RNAs that regulate posttranscriptional gene expression by interacting with target mRNAs. The finely controlled biogenesis, target recognition, and degradation of miRNAs play crucial roles in maintaining cell proliferation, growth, differentiation, and apoptosis. MiRNAs have emerged as potential biomarkers for disease diagnosis, with their expression and activity varying in different contexts such as developmental stages, tissues, and responses to external stimuli.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiaowei Wei, Zehan Cai, Lijun Cai, Yu Wang, Dagan Zhang, Yefei Zhu
Summary: This article introduces a novel multiplex platform for efficient detection of bladder cancer-related microRNAs by integrating photonic crystal barcodes with branched hybridization chain reaction. The platform shows high sensitivity and accuracy, and the results are consistent with conventional detection methods. This method provides a new approach for the clinical diagnosis of bladder cancer.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Chemistry, Analytical
Hua Chai, Yuguo Tang, Zhenzhen Guo, Peng Miao
Summary: This study presents a ratiometric electrochemical biosensor for the detection of circulating tumor DNA (ctDNA). The sensor utilizes DNA probes and DNAzyme activation to achieve detection. The results show high sensitivity and selectivity, and the method holds great potential for widespread application in biological sample analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Taras Pasternak, Ivan A. Paponov, Serhii Kondratenko
Summary: Procedures have been optimized for direct regeneration of entire plants from shoot and root protoplasts of Arabidopsis thaliana, including adjustments to culture media, detection protocols, and optimization of cell reprogramming. Cell division competence was detected through DNA replication events and high cell density, and further optimized by applying glutathione to increase sensitivity to cell cycle activation by auxin. The developed protocol allows investigation into the molecular mechanism of somatic plant cell de-differentiation.
Article
Chemistry, Multidisciplinary
Adrianna N. Masterson, Nayela N. Chowdhury, Yue Fang, Michele T. Yip-Schneider, Sumon Hati, Prashant Gupta, Sha Cao, Huangbing Wu, C. Max Schmidt, Melissa L. Fishel, Rajesh Sardar
Summary: In this study, an advanced nanoplasmonic sensor was developed for the sensitive and amplification-free detection of microRNAs in unprocessed plasma microsamples. The sensor utilized specially designed -ssDNA receptors attached to gold triangular nanoprisms to achieve highly specific detection. The sensor showed an attomolar limit of detection in human plasma and enabled high-throughput assay in a multiwell plate format. The performance of the sensor was validated using clinical samples, demonstrating its ability to differentiate pancreatic ductal adenocarcinoma (PDAC) from other conditions and determine PDAC stages. Furthermore, the sensor showed potential in monitoring microRNA changes in PDAC patients before and after surgery.
Article
Multidisciplinary Sciences
Natthaya Chuaypen, Umaporn Limothai, Pattapon Kunadirek, Pornchai Kaewsapsak, Patipark Kueanjinda, Nattachai Srisawat, Pisit Tangkijvanich
Summary: This study identified miR-601 and miR-630 as significantly up-regulated in patients with icteric leptospirosis, and they were found to be good predictors of subsequent acute liver failure with high sensitivity. Additionally, elevated expression of these miRNAs was also predictive of multi-organ failures and poor overall survival in patients with leptospirosis.
Article
Computer Science, Information Systems
Eghbal Hosseini, Line Reinhardt, Danda B. Rawat
Summary: This article discusses two gradient-based methodologies for solving LP and NLP problems and their efficiency in solving optimization problems with large feasible spaces.
IEEE INTERNET OF THINGS JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
Thomas Jet, Guillaume Gines, Yannick Rondelez, Valerie Taly
Summary: MicroRNA detection is a crucial challenge in analytical chemistry, with the need for high sensitivity, specificity, robustness, and quantitative analysis. The ability to perform multiplexed detection of miRNA concentrations is important for recognizing disease signatures and new innovative detection methods have been developed to address this challenge.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Endocrinology & Metabolism
Hongli Shi, Xin Jiang, Cuidi Xu, Qun Cheng
Summary: This study investigated the differences in miRNA expression profiles in postmenopausal osteoporosis patients with fragility fractures and identified several miRNAs associated with bone mineral density. The study also explored the potential role of these miRNAs in the pathophysiology of postmenopausal osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Claudia Sacchetto, Zenab Mohseni, Robin M. W. Colpaert, Libero Vitiello, Marzia De Bortoli, Indira G. C. Vonhogen, Ke Xiao, Giulia Poloni, Alessandra Lorenzon, Chiara Romualdi, Riccardo Bariani, Elisa Mazzotti, Luciano Daliento, Barbara Bauce, Domenico Corrado, Thomas Thum, Alessandra Rampazzo, Leon J. de Windt, Martina Calore
Summary: The study identified miR-185-5p as significantly upregulated in ARVC patients compared to healthy controls, with a confirmed link to ARVC pathophysiology based on receiver operating characteristic analysis.
Review
Quantum Science & Technology
Guan-Jie Fan-Yuan, Ze-Hao Wang, Shuang Wang, Zhen-Qiang Yin, Wei Chen, De-Yong He, Guang-Can Guo, Zheng-Fu Han
Summary: The decoy-state protocol enables high performance in QKD systems without using single-photon sources. However, a synthesis of different protocols is lacking, making it difficult to determine the optimal protocol. By reviewing and synthesizing advances in the decoy-state method, optimal protocols are identified through the PSO algorithm, providing practical guidelines for various QKD systems.
ADVANCED QUANTUM TECHNOLOGIES
(2021)
Article
Oncology
Jody Vykoukal, Johannes F. Fahrmann, Nikul Patel, Masayoshi Shimizu, Edwin J. Ostrin, Jennifer B. Dennison, Cristina Ivan, Gary E. Goodman, Mark D. Thornquist, Matt J. Barnett, Ziding Feng, George A. Calin, Samir M. Hanash
Summary: Blood-based cancer biomarkers, including miRNA and four protein marker panel, have shown utility in improving the assessment and early detection of lung cancer.
Article
Obstetrics & Gynecology
Hanna Huebner, Hannah Lubrich, Simon Blum, Sophia Antoniadis, Johannes Lermann, Arif Ekici, Peter A. Fasching, Matthias W. Beckmann, Matthias Ruebner, Stefanie Burghaus
Summary: The study compared two extraction methods for isolating and quantifying ccfDNA from endometriosis patients, with the Maxwell method showing significantly higher yield and the QIAamp method showing higher ccfDNA integrity. Both methods are suitable for downstream applications in non-invasive detection and analysis of somatic mutations within endometriosis tissue.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Chemistry, Analytical
Furui Jin, Danke Xu
Summary: The mi-CHA method detects miRNAs by changing the conformation of hairpin structured DNA probes on the chip surface, showing high sensitivity and specificity for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Oncology
Wen-Da Zhou, Lei Shao, Li Dong, Rui-Heng Zhang, Yi-Fan Li, He-Yan Li, Hao-Tian Wu, Xu-Han Shi, Wen-Bin Wei
Summary: Translation: It is significant to establish diagnosis and prognosis evaluation systems for uveal melanoma (UM) patients through imaging techniques. However, imaging examinations lack quantitative biomarkers, making early diagnosis of UM difficult. In this study, six circulating miRNAs were chosen as candidates for independent validation in determining new molecular biomarkers for UM. The results confirmed significantly higher levels of three miRNAs in the serum of UM patients compared to healthy controls, with miR-199a-3p showing the best performance. There were also significant differences in miRNA levels between different types of melanoma and between localized and metastatic UM.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Eduard Claver, Andrea Di Marco, Pamela Frances Brown, Joshua Bradley, Gaetano Nucifora, Alejandro Ruiz-Majoral, Paolo Domenico Dallaglio, Marcos Rodriguez, Josep Comin-Colet, Ignasi Anguera, Christopher A. Miller, Matthias Schmitt
Summary: In patients with non-ischaemic dilated cardiomyopathy (DCM), late gadolinium enhancement limited to the right ventricular insertion points (IP-LGE) is associated with similar outcomes as compared to patients without enhancement (LGE-), but with a significantly lower risk of ventricular arrhythmias and sudden death compared to patients with enhancement (LGE+). Patients with enhancement present at both the right ventricular insertion points and the left ventricle (IP&LV-LGE) have similar clinical characteristics and outcomes as the rest of patients with enhancement.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
(2023)
Article
Cardiac & Cardiovascular Systems
Giulio Falasconi, Diego Penela, David Soto-Iglesias, Pietro Francia, Cheryl Teres, Daniel Viveros, Aldo Bellido, Jose Alderete, Julia Meca-Santamaria, Paula Franco, Augusto Ordonez, Marta Diaz-Escofet, Maria Matiello, Giuliana Maldonado, Claudia Scherer, Marina Huguet, Oscar Camara, Jose-Tomas Ortiz-Perez, Julio Marti-Almor, Antonio Berruezo
Summary: This study aims to evaluate the safety and efficacy of early ventricular tachycardia ablation guided by cardiac magnetic resonance (CMR) in patients with post-myocardial infarction (MI). Patients will be categorized into an ablation group or a medical treatment group based on CMR-derived scar characteristics, and the occurrence of sudden cardiac death or sustained monomorphic ventricular tachycardia will be observed as the primary endpoint. The secondary endpoints include the safety and efficiency outcomes of CMR-guided ablation.
JOURNAL OF INTERVENTIONAL CARDIAC ELECTROPHYSIOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Andrew Sindone, Wolfram Doehner, Josep Comin-Colet
Summary: Intravenous iron-carbohydrate complex supplementation has a positive effect on heart failure patients, reducing hospitalization rates and improving functional and exercise capacity.
Review
Cardiac & Cardiovascular Systems
Melanie Grehn, Stefano Mandija, Marcin Miszczyk, David Krug, Bartlomiej Tomasik, Kristine E. Stickney, Pino Alcantara, Filippo Alongi, Matteo Anselmino, Ricardo Salgado Aranda, Brian Balgobind, Judit Boda-Heggemann, Leif-Hendrik Boldt, Nicola Bottoni, Jakub Cvek, Olgun Elicin, Gaetano Maria De Ferrari, Rutger J. Hassink, Colien Hazelaar, Gerhard Hindricks, Coen Hurkmans, Cinzia Iotti, Tomasz Jadczyk, Otakar Jiravsky, Raphaël Jumeau, Steen Buus Kristiansen, Mario Levis, Manuel Algara Lopez, Julio Marti-Almor, Felix Mehrhof, Ditte Sloth Moller, Giulio Molon, Alexandre Ouss, Petr Peichl, Jiri Plasek, Pieter G. Postema, Aurelio Quesada, Tobias Reichlin, Roberto Rordorf, Boris Rudic, Ardan M. Saguner, Rachel M. A. ter Bekke, Jose Lopez Torrecilla, Esther G. C. Troost, Viviana Vitolo, Nicolaus Andratschke, Katja Zeppenfeld, Slawomir Blamek, Martin Fast, Ludovica de Panfilis, Oliver Blanck, Etienne Pruvot, Joost J. C. Verhoeff
Summary: The STOPSTORM consortium, funded by the EU Horizon 2020 Framework, aims to establish a standardized treatment and outcome platform for investigating STAR in ventricular tachycardia. The consortium consists of 31 clinical and research institutions and is divided into nine work packages. A comprehensive questionnaire was conducted to review the current clinical practice of STAR in Europe, highlighting potential areas of optimization and harmonization.
Article
Cardiac & Cardiovascular Systems
Giulio Falasconi, Diego Penela, David Soto-Iglesias, Pietro Francia, Cheryl Teres, Andrea Saglietto, Beatriz Jauregui, Daniel Viveros, Aldo Bellido, Jose Alderete, Julia Meca-Santamaria, Paula Franco, Carlo Gaspardone, Rodolfo San Antonio, Marina Huguet, Oscar Camara, Jose-Tomas Ortiz-Perez, Julio Marti-Almor, Antonio Berruezo
Summary: The study aimed to personalize the ablation index (AI) based on left atrial wall thickness (LAWT) and investigate its effectiveness in PV antrum isolation for persistent atrial fibrillation (PeAF). The results showed that this personalized approach was effective and efficient, with low procedure, fluoroscopy, and RF time. The importance of this study lies in providing a new method for the treatment of PeAF.
Article
Cardiac & Cardiovascular Systems
Pietro Francia, Daniel Viveros, Giulio Falasconi, Diego Penela, David Soto-Iglesias, Julio Marti-Almor, Jose Alderete, Andrea Saglietto, Aldo Francisco Bellido, Paula Franco-Ocana, Fatima Zaraket, Maria Matiello, Juan Fernandez-Armenta, Rodolfo San Antonio, Antonio Berruezo
Summary: This study assessed the impact of aging on the candidacy and efficacy of cardioneuroablation (CNA) for treating reflex syncope and functional bradyarrhythmia. The results showed that age does not affect the effectiveness of CNA; CNA is an effective method for treating reflex syncope and functional bradyarrhythmia.
Article
Cardiac & Cardiovascular Systems
Helena Tizon-Marcos, Andrea Toloba, Isaac Subirana Cachinero, Roberto Elosua, Alessandro Sionis, Francisco Fernandez-Aviles, Hector Bueno, Andres Carrillo, Antoni Bayes, Pedro L. Sanchez, Merce Roque, Laia Mila, Ane Elorriaga, Jessica Vaquero, Daniel Fernandez-Berges, Daniel Bosch, Javier Alameda, Julio Marti Almor, Manuel Jimenez-Navarro, Luis Martinez, Juan Sanchis, Esther Sanchez, Catalina Rubert, Luis Ruiz-Valdepenas, Marcos Rodriguez, Inigo Lozano, Emad Abu-Assi, Vicente Bertomeu Gonzalez, Jaume Marrugat
Summary: This study analyzed the effect of dual antiplatelet therapy (DAPT) in ST-segment elevation myocardial infarction (STEMI) patients after 1 year. The results showed that there was a significantly higher cardiovascular mortality and cardiovascular readmission or mortality in patients on DAPT compared to those on single antiplatelet therapy at 5 years. It is suggested that extending DAPT up to 5 years in high-risk patients does not improve their long-term prognosis.
REVISTA ESPANOLA DE CARDIOLOGIA
(2023)
Article
Health Care Sciences & Services
Helena Cristobal, Cristina Enjuanes, Montserrat Batlle, Marta Tajes, Begona Campos, Josep Francesch, Pedro Moliner, Marta Farrero, Rut Andrea, Jose Tomas Ortiz-Perez, Albert Morales, Manel Sabate, Josep Comin-Colet, Pablo Garcia de Frutos
Summary: Heart failure (HF) is classified based on left ventricular ejection fraction (EF) into HF with reduced, mildly reduced, and preserved EF. Biomarkers may differ depending on EF type. This study analyzed the levels of soluble form of the AXL receptor tyrosine kinase (sAXL) in HF patients with reduced and preserved EF. The results showed that sAXL levels were a reliable predictor for all-cause and cardiovascular mortality only in the HFpEF cohort.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Health Care Sciences & Services
Josep Comin Colet, Antoni Sicras Mainar, Joel Salazar-Mendiguchia, MariaIsabel del Campo Alonso, Ainara Echeto, David Vilanova Larena, Olga Delgado Sanchez
Summary: This observational and retrospective study analyzed healthcare resources usage and costs associated with nonvalvular atrial fibrillation (NVAF) in Spain. The results showed that compared to acenocoumarol, apixaban reduced the incidence of strokes and systemic embolisms, minor and major bleedings, and deaths. Apixaban also led to significant cost savings in nursing visits, hospitalizations, and emergency visits, with an annual saving of €274 per patient from the perspective of society. Therefore, apixaban is considered a cost-effective alternative for patients with NVAF.
JOURNAL OF COMPARATIVE EFFECTIVENESS RESEARCH
(2023)
Article
Public, Environmental & Occupational Health
Damia Valero-Bover, David Monterde, Gerard Carot-Sans, Miguel Cainzos-Achirica, Josep Comin-Colet, Emili Vela, Montse Cleries, Julia Folguera, Sonia Abilleira, Miquel Arrufat, Yolanda Lejardi, Oscar Solans, Toni Dedeu, Marc Coca, Pol Perez-Sust, Caridad Pontes, Jordi Piera-Jimenez
Summary: The study aimed to assess the contribution of age and comorbidity to the risk of critical illness in hospitalized COVID-19 patients using increasingly exhaustive tools for measuring comorbidity burden. It was found that comorbidity burden explained better than chronological age the increased risk of critical illness observed in patients hospitalized with COVID-19.
CLINICAL EPIDEMIOLOGY
(2023)
Article
Health Care Sciences & Services
Maria del Mar Ras-Jimenez, Raul Ramos-Polo, Josep Francesch Manzano, Miriam Corbella Santano, Herminio Morillas Climent, Nuria Jose-Bazan, Santiago Jimenez-Marrero, Paloma Garcimartin Cerezo, Sergi Yun Viladomat, Pedro Moliner Borja, Blanca Torres Cardus, Jose Maria Verdu-Rotellar, Carles Diez-Lopez, Jose Gonzalez-Costello, Elena Garcia-Romero, Fernando de Frutos Seminario, Laura Triguero-Llonch, Cristina Enjuanes Grau, Marta Tajes Orduna, Josep Comin-Colet
Summary: The soluble transferrin receptor (sTfR) is a marker of tissue iron status that indicates an increased iron demand. This study found that, in non-anemic heart failure patients with normal systemic iron status, higher levels of sTfR were associated with impaired functional capacity and worse quality of life.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Oscar Cano, Victor Bazan, Eduardo Aranad
Summary: This article presents the results of the Spanish catheter ablation registry for the year 2022. The registry saw a significant increase in activity, particularly in atrial fibrillation ablation. Acute success rates improved while complications and mortality rates decreased.
REVISTA ESPANOLA DE CARDIOLOGIA
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jean-Nicolas Dacher, Patrick Langguth, David Adam, Walther-Benedikt Winkler, Julio Marti-Almor, Guenther Prenner, Maria Emilce Trucco, Amir Kol, Meixiang Xiang, Donato Melissano, Hanan Fawaz, Dennis H. Lau
Summary: This study evaluated the safety and performance of trademarked -ENO (R), -TEO (R), or -OTO (R) pacing systems at 1.5 and 3 T MRI, as well as the image quality of non-enhanced MR examinations. The results showed low incidence of MRI-related complications, good electrical stability of the pacing systems, and preserved interpretability of MRI images.
EUROPEAN RADIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Diego Penela, Alfredo Chauca, Juan Fernandez-Armenta, Ricardo Pavon, Begona Benito, Juan Acosta, Jose Miguel Lozano, Giulio Falasconi, Rodolfo San Antonio, David Soto-Iglesias, Julio Marti-Almor, Augusto Ordonez, Aldo Bellido, Jose Miguel Carreno, Maria Matiello, Lucas Cano, Alonso Pedrote, Daniel Viveros, Jose Alderete, Pietro Francia, Maria Algarra-Cullell, Etelvino Silva, Julia Meca-Santamaria, Paula Franco, Riccardo Cappato, Antonio Berruezo
Summary: This study compared the safety, efficacy, and efficiency of single vs. multi-catheter approach for atrial flutter ablation. The results showed that the single-catheter approach is not inferior to the conventional multi-catheter approach, and it has shorter procedure and fluoroscopy time.
JOURNAL OF INTERVENTIONAL CARDIAC ELECTROPHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Diego Penela, Giulio Falasconi, Jose Miguel Carreno, David Soto-Iglesias, Juan Fernandez-Armenta, Juan Acosta, Julio Marti-Almor, Begona Benito, Aldo Bellido, Alfredo Chauca, Claudia Scherer, Daniel Viveros, Jose Alderete, Etelvino Silva, Augusto Ordonez, Jaume Francisco-Pascual, Nuria Rivas-Gandara, Julia Meca-Santamaria, Paula Franco, Carmine De Lucia, Hussam Ali, Riccardo Cappato, Oscar Camara, Pietro Francia, Antonio Berruezo
Summary: This study evaluated the accuracy of a hybrid algorithm (HA) for predicting the origin of OTVAs and developed a new score with improved discriminatory capacity. The results showed that the new score accurately predicted the origin of OTVAs, even in cases with a V3 precordial transition.
JOURNAL OF INTERVENTIONAL CARDIAC ELECTROPHYSIOLOGY
(2023)