Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-06786-9
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Funding
- Engineering and Physical Sciences Research Council [EP/J007404/1]
- Biotechnology and Biological Sciences Research Council [BB/L006979/1, BB/I019464/1, BB/L006804/1]
- BBSRC [BB/P001335/1, BB/R012415/1, BB/L006979/1, BB/L006804/1, BB/I019464/1] Funding Source: UKRI
- EPSRC [EP/J007404/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L006979/1, BB/L006804/1, BB/I019464/1, BB/P001335/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J007404/1] Funding Source: researchfish
- National Biofilms Innovation Centre [NBIC Co-Director MacPhee] Funding Source: researchfish
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BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.
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