Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation

Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new

(2016) Masahiro Sato et al.   Biology Direct

Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

(2016) Masakazu Hashimoto et al.   DEVELOPMENTAL BIOLOGY

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation

(2016) Wenbo Wang et al.   Journal of Genetics and Genomics

Origins of Programmable Nucleases for Genome Engineering

(2016) Srinivasan Chandrasegaran et al.   JOURNAL OF MOLECULAR BIOLOGY

DIANA-mirExTra v2.0: Uncovering microRNAs and transcription factors with crucial roles in NGS expression data

(2016) Ioannis S. Vlachos et al.   NUCLEIC ACIDS RESEARCH

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

(2016) Kazuto Yoshimi et al.   Nature Communications

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes

(2015) M. Crispo et al.   PLoS One

Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins

(2015) Séverine Ménoret et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Simple Genome Editing of Rodent Intact Embryos by Electroporation

(2015) Takehito Kaneko et al.   PLoS One

Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases

(2014) Séverine Remy et al.   GENOME RESEARCH

High-Affinity IgG Antibodies Develop Naturally in Ig-Knockout Rats Carrying Germline Human IgH/Ig /Ig  Loci Bearing the Rat CH Region

(2013) M. J. Osborn et al.   JOURNAL OF IMMUNOLOGY

Intracellular Tracking of Single-plasmid DNA Particles After Delivery by Electroporation

(2013) Christelle Rosazza et al.   MOLECULAR THERAPY

Highly acetylated tubulin permits enhanced interactions with and trafficking of plasmids along microtubules

(2012) M A Badding et al.   GENE THERAPY

Glycosaminoglycans Improves Early Development of Zona-free 8-cell Rat Embryos to Blastocysts in a Chemically Defined Medium, but Not the Pregnancy Rate Following Transfer of the Blastocysts

(2012) Masanobu OKUYAMA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Efficient Delivery of DNA and Morpholinos into Mouse Preimplantation Embryos by Electroporation

(2012) Hui Peng et al.   PLoS One

The Actin Cytoskeleton Has an Active Role in the Electrotransfer of Plasmid DNA in Mammalian Cells

(2011) Christelle Rosazza et al.   MOLECULAR THERAPY

Knockout rats generated by embryo microinjection of TALENs

(2011) Laurent Tesson et al.   NATURE BIOTECHNOLOGY

Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery

(2010) Jean-Michel Escoffre et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Progress and prospects: nuclear import of nonviral vectors

(2010) A P Lam et al.   GENE THERAPY

Tissue-specific and transcription factor-mediated nuclear entry of DNA

(2009) Aaron M. Miller et al.   ADVANCED DRUG DELIVERY REVIEWS

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

Zinc-finger nucleases: a powerful tool for genetic engineering of animals

(2009) Séverine Rémy et al.   TRANSGENIC RESEARCH