Journal
ONCOTARGET
Volume 8, Issue 60, Pages 101475-101488Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.21135
Keywords
galectin-3; thyroid cancer; hypoxia; migration; Src signaling pathway
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Funding
- Programs for National Natural Scientific Foundation of China [81272722, 31370807, 81502004]
- Program for Foundation of Zhongshan Hospital affiliated to Fudan University [2015ZSYXGG12]
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Background: The aim of this study is to investigate the role of Galectin-3 in human thyroid cancer migration. Methods: The expression of Galectin-3 in surgical specimens was investigated using immunohistochemistry and western blot. A papillary thyroid cancer cell line (B-cpap) and an anaplastic thyroid cancer cell line (8305c) were transfected with short-hairpin RNA against Galectin-3 (Gal-3-shRNA). Low-molecular citrus pectin (LCP) was also used to antagonize Galectin-3. The migration and invasion of the cell lines were examined. The related signaling pathways were investigated to explore the Galectin-3 mechanism of action. Results: Galectin-3 was highly expressed in metastasized thyroid cancers. Knocking down and antagonizing Galectin-3 significantly suppressed the migration of thyroid cancer cells. Knocking down Galectin-3 inhibited the activity of Wnt, MAPK, Src and Rho signaling pathways. Galectin-3 was up-regulated via HIF-1 alpha in a hypoxic environment. Galectin-3 knockdown could reduce cell motility in hypoxic environments. Conclusion: This study suggests that Galectin-3 could act as a modulator of thyroid cancer migration, especially in hypoxic microenvironments. This regulation function of Galectin-3 may work through multiple signaling pathways.
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