Chibby 1: a new component of β-catenin-signaling in chronic myeloid leukemia

Chibby 1 (CBY1) is a small and evolutionarily conserved protein, which act as beta-catenin antagonist. CBY1 is encoded by C22orf2 (22q13.1) Its antagonistic function on beta-catenin involves the direct interaction with: The C-terminal activation domain of beta-catenin, which hinders beta-catenin binding with Tcf/Lef transcription factors hence repressing beta-catenin transcriptional activation. 14-3-3 scaffolding proteins (sigma or xi), which drive CBY1 nuclear export into a stable tripartite complex with beta-catenin. The relative proximity of C22orf2 gene encoding for CBY1 to the BCR breakpoint on chromosome 22q11, whose translocation and rearrangement with the c-ABL is the causative event of chronic myeloid leukemia (CML), suggested that gene haploinsufficiency may play a role in the disease pathogenesis and progression. We found CBY1 down-modulation associated with the BCR-ABL1, promoted by transcriptional mechanisms (promoter hyper-methylation) and post-transcriptional events, addressing the protein towards proteasome-dependent degradation through SUMOylation. CBY1 reduced expression in clonal progenitors and, more importantly, in leukemic stem cells (LSC), is contingent upon the tyrosine kinase (TK) activity of BCR-ABL1 fusion protein. Accordingly, its induction by Imatinib (IM) and second generation TK inhibitors contributes to beta-catenin inactivation through multiple events encompassing the activation of endoplasmic reticulum (ER) stress-associated unfolded protein response (UPR) and autophagy, eventually leading to apoptotic death. These findings support the advantage of combined regimens including drugs targeting DNA epigenetics and/or proteasome to eradicate the BCR-ABL1+ hematopoiesis.