Review
Medicine, General & Internal
Amit Akirov, Yaron Rudman
Summary: This study systematically reviewed the role of aromatase inhibitors in the treatment of male prolactinomas with dopamine-agonist-resistant or persistent hypogonadism. The results showed that aromatase inhibitors can reduce estrogen levels, improve sensitivity to dopamine agonists, and control prolactin levels, leading to the shrinking of tumors.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biotechnology & Applied Microbiology
Sundus Kaimari, Archana Kamalakar, Steven L. Goudy
Summary: JAG1 is an important ligand for the NOTCH signaling pathway, necessary for differentiation of stem cells and cranial neural crest cells. However, delivery of soluble JAG1 inhibits NOTCH signaling, so alternative immobilization approaches are being explored. This review discusses the role of JAG1 in disease, its potential as a therapeutic agent, and current delivery methods.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Simona Hankeova, Noemi Van Hul, Jakub Laznovsky, Elisabeth Verboven, Katrin Mangold, Naomi Hensens, Csaba Adori, Elvira Verhoef, Tomas Zikmund, Feven Dawit, Michaela Kavkova, Jakub Salplachta, Marika Sjoqvist, Bengt R. Johansson, Mohamed G. Hassan, Linda Fredriksson, Karsten Baumgartel, Vitezslav Bryja, Urban Lendahl, Andrew Jheon, Florian Alten, Kristina Tear Fahnehjelm, Bjorn Fischler, Jozef Kaiser, Emma R. Andersson
Summary: This study found significant sex differences in spontaneous bleeding events reported by ALGS patients, with more girls than boys experiencing intracranial hemorrhage. Jag1(Ndr/Ndr) mice displayed various vascular defects, and retinography proved to be a non-invasive method to analyze vascular changes in patients.
EMBO MOLECULAR MEDICINE
(2022)
Article
Orthopedics
Jianping Zhang, Na Li, Siyu Lu, Yanling Chen, Lequn Shan, Xingcheng Zhao, Yongqing Xu
Summary: The high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma in 68 clinical specimens. Expression of Jagged1 was significantly higher in F5M2 cells with high metastasis compared to F4 cells with low metastasis. Knockdown of Jagged1 led to reduced proliferation, migration, and invasion abilities in osteosarcoma cells.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2021)
Article
Genetics & Heredity
Laura C. Hernandez-Ramirez, Nathan Pankratz, John Lane, Fabio R. Faucz, Prashant Chittiboina, Denise M. Kay, Zachary Beethem, James L. Mills, Constantine A. Stratakis
Summary: This study aimed to determine the frequency and associated phenotypes of genetic drivers of Cushing's disease (CD). The study found that about 12.2% of patients had variants of interest at the germline level, 7.8% had variants at the somatic level, and 0.4% had coexisting germline and somatic variants.
GENETICS IN MEDICINE
(2022)
Review
Oncology
Noriaki Fukuhara, Mitsuru Nishiyama, Yasumasa Iwasaki
Summary: This review provides an update on recent advances in understanding the pathogenesis, diagnosis, and therapy of prolactinoma. It covers topics such as the effectiveness of dopamine agonists in treating prolactinoma, the recommendation of surgical treatment for patients resistant to dopamine agonists, and the challenges in treating giant and malignant prolactinomas.
Article
Orthopedics
Guangxi Wang, Jinglong Yan, Hao Zhang, Patrick Massey, J. Steven Alexander, Christopher G. Kevil, Shane Barton, Yufeng Dong
Summary: This study demonstrates that Notch activation in vivo can be induced by injecting Jagged1 peptide, leading to enhanced bone callus formation during allograft healing in a femur bone defect mouse model. The translational potential of this therapeutic strategy includes immediate application in chronic long bone defect repair and potential application in enhancing repair of other tissues and organs.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Article
Endocrinology & Metabolism
Kyungwon Kim, Yae Won Park, Daham Kim, Sung Soo Ahn, Ju Hyung Moon, Eui Hyun Kim, Eun Jig Lee, Cheol Ryong Ku
Summary: In patients with visible tumors, the presence of cavernous sinus invasion at diagnosis may be an unfavorable predictor for biochemical remission after CAB discontinuation.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Review
Oncology
Jun Y. Y. Oh, Robert C. C. Osorio, Jangham Jung, Luis Carrete, Nikita Choudhary, Meeki Lad, Atul Saha, Manish K. K. Aghi
Summary: The molecular pathogenesis of pituitary neuroendocrine tumors involves the interplay of many genes and transcription factors in the setting of a diverse altered tumor microenvironment. Recent advancements in molecular technologies, such as single-cell RNA sequencing, have been instrumental in characterizing the transcriptomic profiles of the normal pituitary gland and sporadic PitNETs, providing important insights into their development and potential therapeutic targets.
Article
Oncology
Chaofeng Li, Zhengju Chen, Jinli Gao, Tao Tang, Lei Zhou, Guochao Zhang, Dongdong Zhang, Chao Shen, Lei Guo, Tao Fu
Summary: This study identified the potential role of lncRNA MIR4435-2HG in gastric cancer (GC) tumorigenesis. Inhibition of MIR4435-2HG reduced the viability and migration ability of GC cells. Moreover, delivery of MIR4435-2HG shRNA via exosomes reversed macrophage M2 polarization and inhibited GC tumor growth.
FRONTIERS IN ONCOLOGY
(2022)
Review
Endocrinology & Metabolism
Eduardo J. Medina, Youssef M. Zohdy, Edoardo Porto, Juan M. Revuelta M. Barbero, David Bray, Justin Maldonado, Alejandra Rodas, Miguel Mayol, Bryan Morales, Stewart Neill, William Read, Gustavo Pradilla, Adriana Ioachimescu, Tomas Garzon-Muvdi
Summary: This study reviewed articles and case reports to summarize the molecular abnormalities and management of aggressive prolactinomas (APRLs). Several molecular abnormalities were found to be associated with the aggressiveness of APRLs, and various therapeutic modalities were reviewed for their effectiveness.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Anna Cho, Greisa Vila, Wolfgang Marik, Sigrid Klotz, Stefan Wolfsberger, Alexander Micko
Summary: In patients with small sellar lesions, Prolactin-Volume-Ratios greater than 100 may serve as a potential predictive marker for the diagnosis of prolactin-producing pituitary adenomas.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Clinical Neurology
Robert C. Osorio, Matheus P. Pereira, Taemin Oh, Rushikesh S. Joshi, Alexander F. Haddad, Kaitlyn M. Pereira, Kevin C. Donohue, Zain Peeran, William Carson, Aarav Badani, Elaina J. Wang, Sweta Sudhir, Ankush Chandra, Saket Jain, Angad Beniwal, Jose Gurrola II, Ivan H. El-Sayed, Lewis S. Blevins Jr, Philip V. Theodosopoulos, Sandeep Kunwar, Manish K. Aghi
Summary: This study assessed the correlation between tumor volume and prolactin level in prolactinoma and its impact on surgical outcomes. The results showed a strong association between tumor volume and prolactin levels, with an increase in tumor volume leading to a significant increase in prolactin levels in patients without biochemical remission after surgery.
JOURNAL OF NEUROSURGERY
(2023)
Review
Endocrinology & Metabolism
Shlomo Melmed, Ursula B. Kaiser, M. Beatriz Lopes, Jerome Bertherat, Luis Syro, Gerald Raverot, Martin Reincke, Gudmundur Johannsson, Albert Beckers, Maria Fleseriu, Andrea Giustina, John A. H. Wass, Ken K. Y. Ho
Summary: Pituitary neoplasms are common and mostly benign, but can have diverse effects on health. Understanding the clinical biology of these neoplasms is important for implementing appropriate treatment strategies and improving quality of life and mortality.
Article
Microbiology
Junjie Wen, Chuxi Chen, Meiqun Luo, Xiaocong Liu, Jiading Guo, Tingting Wei, Xinyi Gu, Sinan Gu, Yunshan Ning, Yan Li
Summary: The study highlights the significant role of the Notch signaling pathway in macrophages's response against Helicobacter pylori, with Jagged1 identified as a promoting molecule for bactericidal activities. This finding suggests Jagged1 could be a novel therapeutic target for controlling H. pylori infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Surgery
Charlotte Nys, Georges Versyck, Eveleen Buelens, Koen Engelborghs, Erwin Cornips, Heddy Van Leeuwen-Wintjens, Hugo Vankelecom, Frank Weyns, Diederik Peuskens
Summary: This study retrospectively analyzed 105 patients who underwent transnasal transsphenoidal pituitary surgery for pituitary adenomas and found that the surgery can be performed safely and effectively in a large non-university hospital, improving visual and/or hormonal disturbances and providing long-term tumor control.
ACTA CHIRURGICA BELGICA
(2023)
Article
Multidisciplinary Sciences
Harunobu Kagawa, Alok Javali, Heidar Heidari Khoei, Theresa Maria Sommer, Giovanni Sestini, Maria Novatchkova, Yvonne Scholte Op Reimer, Gael Castel, Alexandre Bruneau, Nina Maenhoudt, Jenna Lammers, Sophie Loubersac, Thomas Freour, Hugo Vankelecom, Laurent David, Nicolas Rivron
Summary: Human pluripotent stem cells cultured in specific conditions can efficiently form blastoids resembling blastocyst-stage analogues, providing a faithful model for investigating human implantation and development.
Article
Biochemistry & Molecular Biology
Lara Hemeryck, Florian Hermans, Joel Chappell, Hiroto Kobayashi, Diether Lambrechts, Ivo Lambrichts, Annelies Bronckaers, Hugo Vankelecom
Summary: Researchers have developed a novel organoid model starting from human tooth, allowing for the exploration of human tooth epithelial stem cell biology and their interaction with dental mesenchyme. These new models may pave the way for future tooth-regenerative perspectives.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Emma Laporte, Silke De Vriendt, Julie Hoekx, Hugo Vankelecom
Summary: Interleukin-6 (IL-6) is important for stem cell activation in the pituitary, but dispensable for normal development and homeostasis. However, IL-6 is needed for the acute stem cell response to local damage in the pituitary.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Guillermina M. Luque, Liza J. Schiavi-Ehrenhaus, Martina Jablonski, Paula A. Balestrini, Analia G. Novero, Nicolas I. Torres, Claudia E. Osycka-Salut, Alberto Darszon, Dario Krapf, Mariano G. Buffone
Summary: Combining fluorescent cell barcoding with drug screening method can be used to quickly screen new CatSper blockers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rui Xu, Hanting Zhao, Jia Qi, Guangxin Yao, Yaqiong He, Yao Lu, Qinling Zhu, Yuan Wang, Ying Ding, Zhenyi Zhu, Xinyu Li, Hugo Vankelecom, Yun Sun
Summary: Overweight significantly impairs IVF outcomes and is often accompanied by hyperglycemia, hyperlipidemia, and metabolic disorders. The relationship between granulosa cell function and metabolic alterations in follicular fluid has been extensively studied. However, the effects of high glucose levels on ovarian granulosa cells are not well understood. This study found that overweight women have elevated follicular glucose levels which activate the NLRP3 inflammasome and pyroptosis. The dysregulation of estradiol synthesis in granulosa cells of overweight patients was also attributed to the activation of the NLRP3 inflammasome and pyroptosis induced by high glucose. This study provides new insights for interpreting and improving IVF outcomes in overweight women.
Article
Medicine, General & Internal
Yun Sun, Linlin Cui, Yao Lu, Jichun Tan, Xi Dong, Tianxiang Ni, Junhao Yan, Yichun Guan, Guimin Hao, Jia-Yin Liu, Bo Zhang, Daimin Wei, Yan Hong, Yaqiong He, Jia Qi, Bing Xu, Juanjuan Lu, Qian Zhang, Shanshan Zhao, Xiaowei Ji, Xiaofang Du, Jie Zhang, Jinyong Liu, Jing Wang, Yingqin Huang, Dongmei Huang, Yanzhi Du, Hugo Vankelecom, Heping Zhang, Zi-jiang Chen
Summary: Prednisone treatment does not improve live birth rate in patients with recurrent implantation failure compared to placebo. The use of prednisone may increase the risk of preterm delivery and biochemical pregnancy loss. These findings challenge the value of prednisone use in clinical practice for the treatment of recurrent implantation failure.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2023)
Article
Endocrinology & Metabolism
Aurelie Hennes, Johanna Devroe, Katrien De Clercq, Martina Ciprietti, Katharina Held, Katrien Luyten, Nele Van Ranst, Nina Maenhoudt, Karen Peeraer, Hugo Vankelecom, Thomas Voets, Joris Vriens
Summary: Early embryo implantation involves a complex process of communication between the blastocyst and the endometrium. Proteases secreted by the blastocyst stimulate calcium signaling pathways in endometrial epithelial cells. This study identified protease-activated receptor 2 (PAR2) as the molecular initiator of calcium responses in endometrial epithelial cells and revealed the downstream signaling pathways involved.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Cell & Tissue Engineering
Florian Hermans, Lara Hemeryck, Celine Bueds, Marc Torres Pereiro, Steffie Hasevoets, Hiroto Kobayashi, Diether Lambrechts, Ivo Lambrichts, Annelies Bronckaers, Hugo Vankelecom
Summary: Organoid models have been successfully established to study tissue biology and development in a dish. In this study, tooth organoids (TOs) were developed from mouse molar and incisor, which express dental epithelium stem cell markers and recapitulate key properties of dental epithelium. These TOs can differentiate into ameloblast-resembling cells and co-differentiate into different cell types in the presence of dental mesenchymal stem cells. Furthermore, the TOs can also survive and differentiate in vivo. These organoid models provide new tools to study mouse tooth biology and development and may have implications for future human tooth repair and replacement.
Review
Endocrinology & Metabolism
Emma Laporte, Hugo Vankelecom
Summary: The pituitary gland is essential for the processes of body growth, fertility, and stress regulation. It contains enigmatic stem cells that require appropriate research models for better understanding of pituitary biology. 3D organoid models derived from pituitary stem cells or pluripotent stem cells have been established for studying pituitary development, biology, and disease.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Endocrinology & Metabolism
Silke De Vriendt, Celia Mesias Casares, Susana Rocha, Hugo Vankelecom
Summary: The uterus-lining endometrium is crucial for mammalian reproduction, but its mechanisms and diseases are not well understood due to its hidden location and lack of suitable research models. Recently, 3D organoid models have been developed to replicate the endometrium, including the reproduction of the menstrual cycle. However, there are limitations in the current use of organoids, such as lack of standardization and xenografting, which hinder clinical translation. Therefore, researchers are seeking better alternatives, such as fully defined matrices, to improve the growth of organoids and advance their application in basic research and clinical fields.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Meeting Abstract
Obstetrics & Gynecology
Xavier Santramaria, Beatriz Roson, Raul Perez, Nanda Kumar, Javier Gonzalez-Fernandez, Estefania Fernandez, Inmaculada Moreno, Hugo Vankelecom, Felipe Vilella, Carlos Simon
REPRODUCTIVE SCIENCES
(2023)
Article
Endocrinology & Metabolism
Shuanggang Hu, Zhe Sun, Boyu Li, Hanting Zhao, Yuan Wang, Guangxin Yao, Xinyu Li, Xuejiao Bian, Tin Chiu Li, Hugo Vankelecom, Yun Sun
Summary: Identification of key molecules involved in endometrial receptivity is crucial for understanding human implantation and improving pregnancy rates in assisted reproduction treatment. This study used proteomics to profile the proteomes of different phases of endometrial tissues and identified differentially expressed proteins. The findings contribute to the understanding of the mechanisms governing endometrial receptivity.
Article
Reproductive Biology
Andrea Romano, Sun-Wei Guo, Jan Brosens, Asgerally Fazlebas, Caroline E. Gargett, Stefan Giselbrecht, Martin Gotte, Linda Griffith, Hugh S. Taylor, Robert N. Taylor, Hugo Vankelecom, Charles Chapron, Xiao-Hong Chang, Khaleque N. Khan, Paola Vigano
Summary: This article presents the protocol for a Delphi process to develop a consensus on in vitro methodologies using endometrial cells. By selecting critical methodologies, topics, and items, and conducting surveys and ratings, unbiased guidance for research methods is promoted to increase confidence in the reliability of in vitro models.
REPRODUCTION AND FERTILITY
(2022)
Article
Biology
Indra Van Zundert, Nina Maenhoudt, Silke De Vriendt, Hugo Vankelecom, Beatrice Fortuni, Susana Rocha
Summary: In recent years, there have been significant advances in the development and implementation of 3D cell models for various applications, including drug development, organogenesis, cancer biology, and personalized medicine. Unlike 2D cell culture systems, advanced 3D cell models provide a more realistic representation of in vivo physiology. However, appropriate investigation techniques are necessary for these models to deliver scientific insights. Fluorescence microscopy has the potential to visualize these models with high spatial resolution, but sample preparation and imaging assays can be challenging.