Article
Cell Biology
Yuhan Hu, Qingzu Gao, Shuai Ma, Pei Yu, Shuang Ding, Xiaofei Yao, Zheying Zhang, Shuya Lu, Manman Lu, Jinghang Zhang, Yanling Wang, Xinlai Qian, Jiateng Zhong
Summary: FMR1, a new m(6)A reader, is upregulated in colorectal cancer (CRC) and promotes tumor growth and metastasis by recognizing the m(6)A modification site in EGFR mRNA, thereby maintaining its stability and expression. This study identifies the METTL3/FMR1/EGFR axis as a potential target for early therapeutic intervention in CRC progression.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Lin Zhang, Yuting Xu, Enmin Cai, Maojin Zheng, Lei Liu, Qingling Wang, Shibao Li
Summary: This study reveals that TSPAN8 is significantly upregulated in gastric cancer tissues and is associated with clinicopathological characteristics and poor prognosis. TSPAN8 promotes cancer cell migration and invasion by activating the EGFR/AKT pathway and regulating EGFR expression. Therefore, TSPAN8 may serve as a promising therapeutic target for gastric cancer.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Oncology
Yingying Zhu, Julien Saint-Pol, Viet Nguyen, Eric Rubinstein, Claude Boucheix, Celine Greco
Summary: Tspan8 is a tetraspanin protein that organizes membrane microdomains and interacts with other membrane molecules, influencing their function. Its expression in digestive tract tumors is associated with a poor prognosis. Tspan8 has been found to enhance the activity of endothelin converting enzyme ECE1, suggesting a role for Tspan8 in modulating the endothelin axis.
Article
Cell Biology
Xiaoqing Lu, Liwei An, Guangjian Fan, Lijuan Zang, Weiyi Huang, Junjian Li, Jun Liu, Weiyu Ge, Yuwei Huang, Jingxuan Xu, Shaoqian Du, Yuan Cao, Tianhao Zhou, Huijing Yin, Li Yu, Shi Jiao, Hongxia Wang
Summary: This study reveals the unconventional function of TSPAN8 and uncovers the molecular mechanisms underlying its nuclear translocation. It demonstrates the role of TSPAN8 in regulating transcription of cancer-promoting genes and identifies it as a potential therapeutic target for refractory cancers.
Review
Biochemistry & Molecular Biology
Sindhu Nair, James A. Bonner, Markus Bredel
Summary: EGFR is overexpressed in head and neck squamous cell carcinoma and anti-EGFR strategies have shown some clinical benefit but often lead to resistance. Mutations in various domains of the EGFR gene can impact drug binding, radiation response, and overall survival in HNSCC patients. Understanding the EGFR mutational landscape and its effects on treatment resistance can help stratify patients for targeted therapies and maximize therapeutic benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Miran Rada, Migmar Tsamchoe, Audrey Kapelanski-Lamoureux, Nour Hassan, Jessica Bloom, Stephanie Petrillo, Diane H. Kim, Anthoula Lazaris, Peter Metrakos
Summary: Vessel co-option is correlated with resistance against anti-angiogenic therapy in colorectal cancer liver metastases. Cancer cells exploit various mechanisms, such as apoptosis, autophagy, motility, and epithelial-mesenchymal transition, to displace hepatocytes and gain access to sinusoidal vessels for vessel co-option.
Article
Neurosciences
Pingping Zhu, Tiankun Lu, Zhenzhen Chen, Benyu Liu, Dongdong Fan, Chong Li, Jiayi Wu, Luyun He, Xiaoxiao Zhu, Ying Du, Yong Tian, Zusen Fan
Summary: This study reveals the importance of intestinal nerve cells in the self-renewal of colorectal cancer stem cells (CSCs) and the development of colorectal tumors. Serotonergic neurons in the enteric system produce serotonin (5-HT) as a regulator of CSC self-renewal. 5-HT interacts with HTR1B/1D/1F receptors to activate Wnt/β-catenin signaling in colorectal CSCs. Additionally, a metabolite derived from the colorectal cancer-enriched microbiota, isovalerate, suppresses the enrichment of the NuRD complex on the Tph2 promoter, leading to the expression of Tph2 and subsequent 5-HT production. 5-HT signaling is correlated with the severity of colorectal cancer.
Article
Oncology
Ying Fu, Xiuzhi Zhang, Xujun Liang, Yongheng Chen, Zhuchu Chen, Zhefeng Xiao
Summary: This study found that CapG is up-regulated in nasopharyngeal carcinoma tissues and cells. CapG promotes migration and invasion of nasopharyngeal carcinoma cells, possibly through the phosphorylation of MLC2. In contrast, Rac1 activation has no significant impact on cell motility in nasopharyngeal carcinoma.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Plant Sciences
Kunjian Peng, Zizheng Zou, Jijia Li, Yuanzhu Xie, Zhengnan Ming, Ting Jiang, Wensong Luo, Xiyuan Hu, Yuan Nie, Ling Chen, Tiao Luo, Ting Peng, Dayou Ma, Suyou Liu, Zhi-Yong Luo
Summary: Spinosyn A (SPA) and its derivative LM2I, derived from a soil microorganism, have potential inhibitory effects on various cancer cells, including colorectal cancer cells. The study demonstrates that SPA and LM2I have significant antiproliferative activity in colorectal cancer cell lines and xenograft tumors, and their inhibitory effects may be mediated through the EGFR pathway.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Xin Zhao, Han Li, Shaocheng Lyu, Jialei Zhai, Zhiwei Ji, Zhigang Zhang, Xinxue Zhang, Zhe Liu, Huaguang Wang, Junming Xu, Hua Fan, Jiantao Kou, Lixin Li, Ren Lang, Qiang He
Summary: The study used single-cell RNA sequencing to reveal the heterogeneity of pancreatic adenosquamous carcinoma (PASC), identifying cell clusters, potential biomarkers, and intercellular interactions, while defining associated signaling pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Medicine, Research & Experimental
Jinming Xu, Yan Wang, Jiahao Jiang, Cong Yin, Bentao Shi
Summary: ADAM12 is significantly upregulated in clear cell renal cell carcinoma (ccRCC) and is associated with poor prognosis. ADAM12 promotes ccRCC cell proliferation, migration, and invasion by activating the EGFR/ERK signaling pathway, inducing epithelial-mesenchymal transition (EMT), and upregulating target genes.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemical Research Methods
Qiong Wu, Jing De Chen, Zhuqing Zhou
Summary: In this study, it was found that high expression of AVL9 in colorectal carcinoma is closely correlated with M status, stages, and poor prognosis of patients. Functionally, AVL9 promotes cell migration. Mechanistically, it regulates EGFR expression to achieve this effect.
BIOLOGICAL PROCEDURES ONLINE
(2022)
Article
Medicine, Research & Experimental
Xishan Chen, Renba Liang, Huan Lin, Kaihua Chen, Li Chen, Ge Tian, Xiaodong Zhu
Summary: Downregulation of CD166 inhibited CSC-related genes and decreased pEGFR and pERK expression in vitro and in vivo. The sphere formation and tumorigenesis ability of CD166-shRNA cells were significantly reduced. Additionally, EGF-stimulated CD166-shRNA cells showed an increase in CSC-like traits by activating the EGFR/ERK1/2 signaling pathway.
Article
Pharmacology & Pharmacy
Hyemin Lee, Duen-Yi Huang, Hua-Ching Chang, Chia-Yee Lin, Wan-Yu Ren, Yang-Shia Dai, Wan-Wan Lin
Summary: B lymphocyte-induced maturation protein-1 (Blimp-1) can be transcriptionally upregulated by various stimuli in keratinocytes and squamous cell carcinoma (SCC) via EGFR transactivation and the AP-1 pathway. The nuclear localization of Blimp-1 remains unchanged under stimulation, and silencing Blimp-1 can enhance SCC cell migration. These findings suggest a potential therapeutic target in SCC by targeting Blimp-1 as a negative regulator of cell migration.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Rumeysa Biyik-Sit, Traci Kruer, Susan Dougherty, James A. Bradley, Daniel W. Wilkey, Michael L. Merchant, John O. Trent, Brian F. Clem
Summary: In addition to its traditional role in cellular serine synthesis pathway, PSAT1 has been shown to participate in lung cancer cell migration, mediated by its interaction with PKM2.
Article
Oncology
Danielle R. Bond, Richard Kahl, Joshua S. Brzozowski, Helen Jankowski, Crystal Naudin, Mamta Pariyar, Kelly A. Avery-Kiejda, Christopher J. Scarlett, Claude Boucheix, William J. Muller, Leonie K. Ashman, Murray J. Cairns, Severine Roselli, Judith Weidenhofer
Article
Oncology
Manale El Kharbili, Muriel Cario, Nicolas Bechetoille, Catherine Pain, Claude Boucheix, Francoise Degoul, Ingrid Masse, Odile Berthier-Vergnes
Article
Multidisciplinary Sciences
Anne-Claire Langlois, Giulia Manzoni, Laetitia Vincensini, Romain Coppee, Carine Marinach, Maryse Guerin, Thierry Huby, Veronique Carriere, Francois-Loic Cosset, Marlene Dreux, Eric Rubinstein, Olivier Silvie
SCIENTIFIC REPORTS
(2020)
Letter
Dermatology
E. Moreno Artero, N. Schinkel, S. Chaumon, I. Corset, T. Rabeony, C. Elie, N. Bellon, C. Bodemer, C. Greco
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laurent Fernandez, Morgane Malrieu, Christine Benistant, Patrice Dosset, Eric Rubinstein, Elena Odintsova, Fedor Berditchevski, Pierre-Emmanuel Milhiet
Summary: Tetraspanins are a family of transmembrane proteins that control the lateral segregation of their partners at the plasma membrane through mechanisms involving specific lipids. CD82 expression increases the dynamics of CD81 and alters its localization at the plasma membrane in mammary epithelial cells, while having no effect on CD9 behavior.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Mathilde Mathieu, Nathalie Nevo, Mabel Jouve, Jose Ignacio Valenzuela, Mathieu Maurin, Frederik J. Verweij, Roberta Palmulli, Danielle Lankar, Florent Dingli, Damarys Loew, Eric Rubinstein, Gaelle Boncompain, Franck Perez, Clotilde Thery
Summary: This study tracks the intracellular trafficking of two proteins before their secretion in EVs, reporting the biogenesis and protein markers of EV subtypes: ectosomes budding from the plasma membrane as well as exosomes from late endosomes.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
David Machover, Wathek Almohamad, Vincent Castagne, Christophe Desterke, Lea Gomez, Yann Gaston-Mathe, Claude Boucheix, Emma Goldschmidt
Summary: The supplementation of high-dose vitamin B6 enhances the anti-tumor potency of regimens containing FUra and FA in patients with digestive tract carcinoma, leading to improved treatment response rates.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Maelle Givelet, Virginie Firlej, Bruno Lassalle, Anne Sophie Gille, Clementine Lapoujade, Isabelle Holtzman, Amandine Jarysta, Farahd Haghighirad, Florent Dumont, Sebastien Jacques, Franck Letourneur, Francoise Pflumio, Isabelle Allemand, Catherine Patrat, Nicolas Thiounn, Jean Philippe Wolf, Lydia Riou, Virginie Barraud-Lange, Pierre Fouchet
Summary: Researchers have established an exhaustive transcriptome of human spermatogenesis by analyzing the characteristics of human germ cells, revealing the molecular networks and key transcriptional regulators involved in the regulation of human spermatogonial stem cells.
Article
Biochemistry & Molecular Biology
Celine Greco, Anne-Charlotte Ponsen, Stephanie Leclerc-Mercier, Joel Schlatter, Salvatore Cisternino, Claude Boucheix, Christine Bodemer
Summary: This study investigated the possibility of using erlotinib and lapatinib to treat painful palmoplantar keratoderma in patients with pachyonychia congenita. The results showed that oral erlotinib at a dose of 75 mg/day could alleviate pain and reduce hyperkeratosis to some extent. Oral lapatinib, while reducing pain and improving symptoms, also had side effects. The effectiveness of erlotinib cream was inconclusive. Oral Her1 or 1/2 inhibitors offer promising therapeutic perspectives for patients with pachyonychia congenita.
Article
Cell Biology
Vera-Marie E. Dunlock, Abbey B. Arp, Simar Pal Singh, Stephanie Charrin, Viet Nguyen, Erik Jansen, Fleur Schaper, Martin Ter Beest, Malou Zuidscherwoude, Sjoerd J. Van Deventer, Britt Nakken, Peter Szodorey, Maria C. Demaria, Mark D. Wright, Laia Querol Cano, Eric Rubinstein, Annemiek B. Van Spriel
Summary: T cells rely on the phosphatase CD45 and its partner CD53 for optimal signaling and activation, with CD53 controlling CD45 function and stability on the cell membrane. Understanding the mechanisms of CD45 regulation and CD53-mediated T cell immunity is crucial for advancing our knowledge of immune responses.
Article
Cell Biology
Ye Fan, Cedric Pionneau, Federico Cocozza, Pierre-Yves Boelle, Solenne Chardonnet, Stephanie Charrin, Clotilde Thery, Pascale Zimmermann, Eric Rubinstein
Summary: This study reveals that CD9, CD81, and CD63, commonly used as protein markers for extracellular vesicles (EVs), have little impact on the protein composition of EVs released by MCF7 breast cancer cells. Knocking out CD9 and CD81 only significantly decreases the levels of CD9P-1 and EWI-2 in EVs. This suggests a marginal role of CD9, CD81, and CD63 in determining the protein composition of EVs and highlights the regulation of CD9P-1 and EWI-2 expression level and/or trafficking by CD9 and CD81.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Immunology
Stephanie Charrin, Roberta Palmulli, Martine Billard, Denis Clay, Claude Boucheix, Guillaume Van Niel, Eric Rubinstein
Article
Biology
Etienne Eschenbrenner, Stephanie Jouannet, Denis Clay, Joelle Chaker, Claude Boucheix, Christel Brou, Michael G. Tomlinson, Stephanie Charrin, Eric Rubinstein
LIFE SCIENCE ALLIANCE
(2020)