Article
Medicine, General & Internal
Yafang Li, Zhichuang Lian, Qifeng Li, Wei Ding, Wenyi Wang, Ling Zhang, Xirennayi Muhataer, Yuan Zhou, Xiaohong Yang, Chao Wu
Summary: This study investigated the molecular mechanisms of Notch signaling and autophagy in the development of pigeon breeder's lung (PBL) pulmonary fibrosis. The results showed that Notch signaling regulates autophagy and is involved in the pathological process of PBL pulmonary fibrosis.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Cell Biology
Yanhui Li, Ying Sun, Nan Wu, Haichun Ma
Summary: This study reveals the significant role of GRK2 in the development of pulmonary fibrosis. Inhibition of GRK2 attenuates the pathological features of pulmonary fibrosis, possibly through suppression of Smad3 expression. This suggests that GRK2 may be a potential therapeutic target for treating pulmonary fibrosis.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Drew F. K. Williamson, Sean R. N. Marris, Vanesa Rojas-Rudilla, Jacqueline L. Bruce, Cloud P. Paweletz, Geoffrey R. Oxnard, Lynette M. Sholl, Fei Dong
Summary: Activating mutations in EGFR can predict the effectiveness of tyrosine kinase inhibitors in treating advanced non-small cell lung cancer. This article presents a fast and accurate analytical method for detecting EGFR mutations.
Article
Biochemistry & Molecular Biology
Hailong Li, Jinhe Li, Yayue Hu, Ruotong Zhang, Xiaoting Gu, Yiying Wei, Shanshan Zhang, Xuefen Chen, Luqing Wei, Xiaohe Li, Songtao Gu, Jin Jin, Hui Huang, Honggang Zhou, Cheng Yang
Summary: This study found that FOXO3 has binding sequences with the SPON1 promoter, which activates the transcription of SPON1 circRNA and promotes its expression. The circSPON1 is involved in the extracellular matrix deposition and inhibits fibroblast activation by interacting with Smad3 and interfering with Smad7 mRNA. These findings provide new insights into the mechanism of FOXO3-regulated circSPON1 in pulmonary fibrosis and potential therapeutic targets for idiopathic pulmonary fibrosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Environmental Sciences
Naresh Singh, Naveen Arora
Summary: The study found that exposure to diesel exhaust in mice led to lung injury and pulmonary fibrosis, as evidenced by impaired lung function, cellular infiltration, inflammation, thickening of the alveolar wall, and tissue remodeling. The involvement of the TGF-beta/SMAD3 pathway and activation of EMT in diesel exhaust-exposed mice was demonstrated.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Sudheendran Mavila, Heidi R. Culver, Alex J. Anderson, Tania R. Prieto, Christopher N. Bowman
Summary: An athermal approach using self-immolative thioester linked nucleic acids (TENA) for mRNA enrichment from total RNA has shown comparable performance to a commercial kit. The method is compatible with downstream applications such as RT-qPCR for gene expression analysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Medicine, General & Internal
Edinson Dante Meregildo-Rodriguez, Martha Genara Asmat-Rubio, Gustavo Adolfo Vasquez-Tirado
Summary: Tuberculosis is a global public health emergency. Improved diagnostic methods are urgently needed to address this epidemic. Droplet digital PCR (ddPCR) shows comparable diagnostic performance to qPCR for pulmonary tuberculosis, and better performance for extrapulmonary tuberculosis.
FRONTIERS IN MEDICINE
(2023)
Article
Infectious Diseases
Ruben Morilla, Francisco J. Medrano, Ana Calzada, Esther Quintana, Elena Campano, Vicente Friaza, Enrique J. Calderon, Carmen de la Horra
Summary: This study suggests for the first time the possible transmission of Pneumocystis in the family environment of patients with cystic fibrosis, indicating that patients and their household members are reservoirs and possible sources of this infection.
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Allergy
Chao Wu, Wei Ding, Yafang Li, Wenyi Wang, Zhichuang Lian, Xirennayi Muhataer, Yuan Zhou, Qifeng Li, Xiaohong Yang
Summary: In this study, we investigated the molecular mechanism by which B lymphocytes regulate Th1/Th2 imbalance in hypersensitivity pneumonia-induced pulmonary fibrosis. Our results showed that B cells play a key role in inducing pneumonia, and the Notch signaling pathway is involved in regulating the Th1/Th2 ratio.
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Fan Yang, Zhen-feng Hou, Hao-yue Zhu, Xiao-xuan Chen, Wan-yang Li, Ren-shuang Cao, Yu-xuan Li, Ru Chen, Wei Zhang
Summary: This study identified key processes and signaling pathways involved in the development of IPF, suggesting the potential therapeutic value of CAT in treating IPF by targeting collagen metabolism imbalance, inflammatory response, and EMT process.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, General & Internal
Jeong-Hwa Kim, Jae-Woon Oh, Young Lee, Jeong-Ho Yun, Seong-Ho Choi, Dong-Woon Lee
Summary: This study found that detecting 18 bacterial species in mouth-rinse samples can be used to diagnose periodontitis, with Treponema denticola, Prevotella nigrescens, and Streptococcus mitis significantly associated with severe periodontitis. Further research is needed to assess the potential of oral-rinsing solutions in indicating oral infection risk and improving oral hygiene.
JOURNAL OF CLINICAL MEDICINE
(2021)