Article
Immunology
Johannes Dirks, Oliver Andres, Luisa Paul, Georgi Manukjan, Harald Schulze, Henner Morbach
Summary: The expression of IgD in human naive B cells has a significant impact on their maturation and Ig selection, shaping the composition of naive B cells and being involved in selecting specific Ig V-H segments for pre-immune Ig repertoire. Naive B cells lacking IgD expression showed decreased responsiveness in vitro to signals from IgM-BCR, TLR7/9, or CD40 pathways. Furthermore, the frequency of IgM(lo/-) B cells lacking IgD was significantly reduced in the mature naive B cell compartment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Timm Amendt, Omar El Ayoubi, Alexandra T. Linder, Gabriele Allies, Marc Young, Corinna S. Setz, Hassan Jumaa
Summary: IgD-deficient mice have an accelerated rate of B cell responsiveness and can activate antibody production within 24 hours after immunization, while wildtype mice require 3 days to activate antibody responses. IgD-deficient mice are unable to modulate antibody production, suggesting that IgD controls the activation rate of B cells and subsequent antibody production by sensing and distinguishing antigen valences.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Timm Amendt, Hassan Jumaa
Summary: The study indicates that autoreactive antibodies can be easily generated in wild-type animals, but adaptive tolerance can prevent autoimmune diseases by inducing affinity-matured IgM specific to self-antigens. This suggests that B-cell tolerance does not necessarily involve the complete elimination of autoreactive specificities, as inducible generation of autoantigen-specific high-affinity IgM serves as a protective mechanism against self-destruction.
Article
Biochemistry & Molecular Biology
Deniz Saltukoglu, Bugra Oezdemir, Michael Holtmannspoetter, Ralf Reski, Jacob Piehler, Rainer Kurre, Michael Reth
Summary: Using lattice light-sheet microscopy and tailored custom-built 4D image analysis, the cell-surface topography of Ramos B cells and the spatiotemporal organization of IgM-BCR were studied. Dynamic ridge-network organization and IgM-BCR cluster mobility were found to be linked and controlled by Arp2/3 complex activity. The results suggest that dynamic topographical features of the cell surface govern the localization and transport of IgM-BCR clusters for antigen screening by B cells.
Article
Immunology
Katrin Schoenfeld, Julia Harwardt, Jan Habermann, Adrian Elter, Harald Kolmar
Summary: This article presents a novel approach using specific antibody-drug conjugates to eliminate malignant B cells while maintaining a functional adaptive immune system, providing a new method for non-Hodgkin's lymphoma treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Qiang Su, Mengying Chen, Yan Shi, Xiaofeng Zhang, Gaoxingyu Huang, Bangdong Huang, Dongwei Liu, Zhangsuo Liu, Yigong Shi
Summary: This study reports the cryo-electron microscopy structure of human IgM-BCR in the resting state and reveals the organizational principles of BCR. The findings may facilitate the design of antibody-based therapeutics.
Article
Immunology
Zoe Steier, Dominik A. Aylard, Laura L. McIntyre, Isabel Baldwin, Esther Jeong Yoon Kim, Lydia K. Lutes, Can Ergen, Tse-Shun Huang, Ellen A. Robey, Nir Yosef, Aaron Streets
Summary: Streets, Yosef, Robey and colleagues used multiomics analysis to establish a comprehensive timeline of the development of CD4(+) and CD8(+) T cells lineage commitment. They identified sequential waves of TCR signaling that initiate CD4(+) T cell lineage differentiation followed by CD8(+) T cell lineage specification. Their findings provide valuable insights into cell fate decisions and suggest a sequential selection process in guiding lineage choice.
Article
Immunology
Christopher M. Skopnik, Khlowd Al-Qaisi, Rosaleen A. Calvert, Philipp Enghard, Andreas Radbruch, Brian J. Sutton, Hiromi Kubagawa
Summary: This study identified critical amino acid residues of human Fc mu R for binding to IgM Fc, as well as the effects of certain residues on receptor expression and IgM binding. Results suggest specific sites and stretches within the Fc mu R structure that are essential for its proper function and interaction with IgM.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Diana Martin, M. Camino Ordas, Ines Carvalho, Patricia Diaz-Rosales, Noelia Nunez-Ortiz, Samuel Vicente-Gil, Aitor Arrogante, Carlos Zarza, Marina Machado, Benjamin Costas, Carolina Tafalla
Summary: This study investigates the effects of methionine, tryptophan, and lysine on the functionality of rainbow trout IgM(+) B cells. The results show that methionine supplementation significantly increases cell proliferation and IgM secretion, while tryptophan and lysine have minor or even negative effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jarred M. Whitlock, Evgenia Leikina, Kamran Melikov, Luis Fernandez De Castro, Sandy Mattijssen, Richard J. Maraia, Michael T. Collins, Leonid V. Chernomordik
Summary: In this study, it was found that the nuclear RNA chaperone La protein plays a role in regulating osteoclast fusion. The levels of La protein decrease during monocyte-to-osteoclast differentiation but reappear as a low molecular weight form at the surface of fusing osteoclasts, promoting fusion. The fusion-promoting role of La protein is independent of its canonical interactions with RNA and involves interactions with Annexin A5.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Samyuktha Ramesh, Soohyung Park, Wonpil Im, Melissa J. Call, Matthew E. Call
Summary: The B cell receptor (BCR) and T cell receptor (TCR) share a common core transmembrane (TM) structure, which is vital for optimal receptor assembly and stability in the cell membrane.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Nicolas Rochereau, Eva Michaud, Louis Waeckel, Martin Killian, Remi Gayet, Roman Goguyer-Deschaumes, Xavier Roblin, Gilles Biolley, Blaise Corthesy, Stephane Paul
Summary: Recent studies have shown that SIgM can also undergo retrotranscytosis through M cells, indicating a potential role in regulating mucosal immunity.
Article
Immunology
Fauna L. L. Smith, Hannah P. P. Savage, Zheng Luo, Christopher M. M. Tipton, F. Eun-Hyung Lee, April C. C. Apostol, Anna E. E. Beaudin, Diego A. A. Lopez, Ingvill Jensen, Stefan Keller, Nicole Baumgarth
Summary: Natural (n)IgM is an evolutionarily conserved substance that reacts to both self and foreign antigens. Its deficiency can lead to an increase in autoimmune diseases and infections. Recent studies have revealed that nIgM is produced by different types of B-1 cells, generating distinct repertoires, and BM nIgM-secreting B-1 plasma cells require the presence of TCR alpha beta(+) CD4 T cells for their development. The repertoire of BM nIgM-secreting B-1 plasma cells contains public clones with short Igh-CDR3 motifs.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Fisheries
Jianmin Ye, Lan Li, Chenxi Duan, Liting Wu, Xiao Tu, Mary Ann Vogelbein, Erin Bromage, Stephen L. Kaattari
Summary: It was found that high antigen concentrations could significantly compromise IgM antibody titers and affinities in rainbow trout. Pulsed antigen cultures provided more accurate IgM antibody evaluations, and high antigen concentrations specifically suppressed high affinity B cell induction. Optimal expression of high affinity antibodies required exposure to lower concentrations of antigen.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Hongkai Wu, Zhichao Zhou, Shi Xie, Rong Yan, Mingxing Gong, Xingui Tian, Zhanhui Wang
Summary: The study measured the B cell receptor (BCR) repertoire similarity in blood and spleen samples from BALB/c and C57BL/6J mice, finding better convergence in IgM compared to IgA and IgG, and better robustness in IgM somatic mutation networks. It also showed better sharing of CDR3 clonotypes and clonal lineages in spleen samples than in blood samples. Differences in CDR3 evenness, mutated clonotype proportions, and maximal network degrees were detected between animal batches.