Article
Immunology
Rebecca Isabel Wurm-Kuczera, Judith Buentzel, Julia Felicitas Leni Koenig, Tobias Legler, Jan-Jakob Valk, Justin Hasenkamp, Wolfram Jung, Jan-Gerd Rademacher, Peter Korsten, Gerald Georg Wulf
Summary: Sarcoidosis is a disease characterized by altered immune response and inflammatory granulomatous tissue infiltration. It may occur during immune reconstitution after HSCT, with glucocorticoid treatment resolving symptoms effectively. The HLA allele association reflects that observed in the general population.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Cell Biology
Frantisek Mrazek, Sarka Pubalova, Zbynek Novak, Nikola Mojtkova, Milena Vrana
Summary: HLA-A*29:172 allele differs from HLA-A*29:01:01:01 by one missense single C/G nucleotide exchange in codon 77.
Review
Immunology
Tiira Johansson, Jukka Partanen, Paeivi Saavalainen
Summary: Varying levels of HLA allele-specific expression are associated with human diseases, and RNA sequencing-based approaches with NGS methodologies have emerged to quantify HLA allele-specific expression and provide important information for predicting immune cell therapy efficacy.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Scott M. Krummey, Alison J. Gareau
Summary: Advances in hematopoietic stem cell transplant have led to changes in donor selection approach, including the use of haploidentical donors or permissive HLA mismatches. However, this may increase the risk of donor-specific HLA antibodies, which can limit engraftment. Desensitization treatments can effectively reduce HLA antibodies and improve transplant outcomes. The consideration and management of HLA antibodies in donor selection are increasingly important in HSCT.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Thomas Fehr, Kerstin Huebel, Olivier de Rougemont, Irene Abela, Ariana Gaspert, Tayfun Guengoer, Mathias Hauri, Birgit Helmchen, Claudia Linsenmeier, Thomas Mueller, Jakob Nilsson, Oliver Riesterer, John D. Scandling, Urs Schanz, Pietro E. Cippa
Summary: This study reports the first European clinical trial on kidney transplantation aiming to induce immunological tolerance through mixed lymphohematopoietic chimerism. The results demonstrate that combined kidney and hematopoietic stem cell transplantation from an HLA-matched sibling is a feasible and successful approach, while maintaining immune responsiveness to mRNA vaccines.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Hongfeng Huang, Qixia Shen, Jingyi Zhou, Xiuyan Yang, Qiuqin Cai, Jia Shen, Shi Feng, Wenqing Xie, Hong Jiang, Jianghua Chen
Summary: The clinical experience demonstrates the efficacy of non-myeloablative conditioning protocol in inducing tolerance in HLA identical patients, and complete chimerism may be a risk factor for GVHD.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Ding-Ping Chen, Su-Wei Chang, Po-Nan Wang, Wei-Tzu Lin, Fang-Ping Hsu, Wei-Ting Wang, Ching-Ping Tseng
Summary: The study revealed significant associations between SNPs in co-stimulatory genes and adverse outcomes post-HSCT, suggesting a potential role of these SNPs in immune responses to grafts post-HSCT and providing potential targets for identifying appropriate donors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Chrysanthi Tsamadou, Daphne Engelhardt, Uwe Platzbecker, Elisa Sala, Thomas Valerius, Eva Wagner-Drouet, Gerald Wulf, Nicolaus Kroeger, Niels Murawski, Hermann Einsele, Kerstin Schaefer-Eckart, Sebastian Freitag, Jochen Casper, Martin Kaufmann, Mareike Duerholt, Bernd Hertenstein, Stefan Klein, Mark Ringhoffer, Sandra Frank, Christine Neuchel, Hubert Schrezenmeier, Joannis Mytilineos, Daniel Fuerst
Summary: Mismatches at the HLA-DRB3/4/5 loci may lead to an increased risk of severe graft-versus-host disease (GvHD) post-transplantation, particularly in otherwise fully matched transplant pairs. The study results suggest that mismatches at the HLA-DRB3/4/5 loci are associated with adverse outcomes in unrelated hematopoietic stem cell transplantation (uHSCT).
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Hematology
Effie W. Petersdorf, Mats Bengtsson, Mary Horowitz, Caroline McKallor, Stephen R. Spellman, Eric Spierings, Ted A. Gooley, Phil Stevenson
Summary: HLA-DQ heterodimers influence susceptibility to autoimmune diseases, but their role in hematopoietic cell transplantation is unknown. This study found that the presence of G2 heterodimers increased the risk of relapse in HLA-matched and HLA-DQ-mismatched patients undergoing transplantation, and the risk increased with a higher number of G2 molecules.
Review
Cell & Tissue Engineering
Davod Pashoutan Sarvar, Hosein Effatpanah, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: The study highlights the important role of MSC-derived extracellular vesicles (MSC-EVs) in hematopoietic regulation and immune response after transplantation. MSC-EVs directly fuse with hematopoietic stem cell membranes, affecting their fate, and regulate the proliferation and differentiation of hematopoietic stem cells through the release of various bioactive factors.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Immunology
Yanmin Zhao, Fei Gao, Yibo Wu, Jimin Shi, Yi Luo, Yamin Tan, Jian Yu, Xiaoyu Lai, Mingming Zhang, Wei Zhang, He Huang
Summary: Research suggests that decreased iKIR-HLA C pairing post-transplantation may lead to weakened NK cell function and increased risk of relapse. Patients undergoing haploidentical HSCT with decreased iKIR-HLA C pairs post-transplantation are associated with higher relapse rates and reduced survival outcomes, particularly for those with myeloid diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Tirthankar Sen, Rajkumar P. Thummer
Summary: The human microbiota plays a significant role in organ transplantation outcomes and rejection risk, with complex interactions existing between different microbiotas and the human body.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Soheila Montazersaheb, Ali Ehsani, Ezzatollah Fathi, Raheleh Farahzadi, Ilja Vietor
Summary: Autophagy is a fundamental process that helps cells adapt to metabolic stress by degrading and recycling intracellular components. It plays a crucial role in various aspects of allogeneic hematopoietic stem cell transplantation (HSCT), including the survival and function of hematopoietic stem cells, T-cell differentiation, antigen presentation, and responsiveness to cytokine stimulation. However, the overall role of autophagy in HSCT remains unclear, highlighting the need for further understanding to improve transplant outcomes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Immunology
Ashley M. Nelson, Alexandra A. Erdmann, Christopher L. Coe, Mark B. Juckett, Keayra Morris, Jennifer M. Knight, Peiman Hematti, Erin S. Costanzo
Summary: Increased synthesis and release of inflammatory signalling proteins are common among individuals with hematologic malignancies undergoing hematopoietic cell transplantation (HCT). This study examined the relationship between markers of inflammatory activity and depression symptoms following HCT. The results showed that patients with elevated levels of IL-6 and IL-10 reported more severe depression symptoms post-HCT, especially in terms of neurovegetative symptoms.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Immunology
Ding-Ping Chen, Po-Nan Wang, Ai-Ling Hour, Wei-Tzu Lin, Fang-Ping Hsu, Wei-Ting Wang, Ching-Ping Tseng
Summary: In addition to classical HLA genes, the outcomes of post-HSCT are influenced by HLA-related genes and non-HLA genes involved in immune regulation. The HLA-G gene is crucial for immune tolerance, tumor cell escape, and transplant rejection reduction. This study investigates the association between genetic variants in the 3'-UTR and 5'-URR of HLA-G and adverse outcomes in leukemia patients receiving HSCT. Specific SNPs and haplotypes were found to be linked to the occurrence of chronic graft-versus-host disease (GVHD). These findings suggest that these genetic variants may serve as predictors for poor outcomes after HSCT and guide preventive treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Chantal Reina-Ortiz, Michael Constantinides, Alexis Fayd-Herbe-de-Maudave, Jessy Presumey, Javier Hernandez, Guillaume Cartron, Rosana Diez, Isabel Izquierdo, Gemma Azaceta, Luis Palomera, Isabel Marzo, Javier Naval, Alberto Anel, Martin Villalba, David Giraldosa
Summary: The study evaluated the potential of expanded NK cells from two sources combined with mAbs to target primary multiple myeloma cells. Results indicate that umbilical cord blood eNKs are highly cytotoxic against MM cells, while peripheral blood eNKs have significant cytotoxic advantage when combined with daratumumab.
Article
Multidisciplinary Sciences
Karim Mrouj, Nuria Andres-Sanchez, Geronimo Dubra, Priyanka Singh, Michal Sobecki, Dhanvantri Chahar, Emile Al Ghoul, Ana Bella Aznar, Susana Prieto, Nelly Pirot, Florence Bernex, Benoit Bordignon, Cedric Hassen-Khodja, Martin Villalba, Liliana Krasinska, Daniel Fisher
Summary: Ki-67, while not necessary for cell proliferation, plays a crucial role in tumor initiation, growth, and metastasis. Its absence leads to changes in the transcriptome and immune response, making cancer cells more susceptible to drugs and immune attacks.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Alexis Fayd'herbe De Maudave, Wilhem Leconet, Karine Toupet, Michael Constantinides, Guillaume Bossis, Marion de Toledo, Jerome Vialaret, Christophe Hirtz, Adolfo Lopez-Noriega, Christian Jorgensen, Daniele Noel, Pascale Louis-Plence, Sylvestre Grizot, Martin Villalba
Summary: Monoclonal antibodies have high therapeutic potential for cancer or autoimmune diseases, but intravenous administration can lead to off-target effects. Utilizing a local controlled-delivery approach with in situ forming depot technology can help address this issue.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Cell Biology
Chantal Reina-Ortiz, David Giraldos, Gemma Azaceta, Luis Palomera, Isabel Marzo, Javier Naval, Martin Villalba, Alberto Anel
Summary: NK cell-based therapies show promise as anticancer treatments. The review compares autologous and allogeneic NK cell treatments for MM and their combination with existing therapies. The placement of these treatments in clinical regimens is discussed based on patient immune profiles.
Article
Multidisciplinary Sciences
Sana Belkahla, Joaquin Marco Brualla, Alexis Fayd'herbe de Maudave, Paolo Falvo, Nerea Allende-Vega, Michael Constantinides, Abrar Ul Haq Khan, Lois Coenon, Catherine Alexia, Giulia Mitola, Paul Massa, Stefania Orecchioni, Francesco Bertolini, Wissem Mnif, Javier Hernandez, Alberto Anel, Martin Villalba
Summary: Leukemic cells adapt their metabolism to support their fast proliferation and can be recognized by immune cells through activating receptors. The tumor suppressor gene p53 plays a role in cell metabolism and the expression of ligands involved in immune recognition. A chemical compound called dichloroacetate (DCA) induces the expression of these ligands in tumor cells with functional p53, sensitizing them to cytotoxic lymphocytes. DCA treatment also slows down tumor growth in vivo and could potentially enhance the effectiveness of immunotherapies using CAR T cells or NK cells.
SCIENTIFIC REPORTS
(2022)
Review
Immunology
Lois Coenon, Martin Villalba
Summary: This review provides an overview of the latest strategies employed to improve antibody-dependent NK cell cytotoxicity, by enhancing the biological function of CD16a receptor.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Abrar Ul Haq Khan, Hamideh Salehi, Catherine Alexia, Jose M. Valdivielso, Milica Bozic, Isabel C. Lopez-Mejia, Lluis Fajas, Sabine Gerbal-Chaloin, Martine Daujat-Chavanieu, Delphine Gitenay, Martin Villalba
Summary: This study shows that glucose starvation or sustained pyruvate dehydrogenase activation can induce genetic remodeling to enhance fatty acid oxidation in cells. The signaling pathway MEK5/ERK5 plays a key role in this process, regulating lipid transport, activation of acyl-CoA synthetase long-chain family members, and induction of acyl-CoA dehydrogenases.
Article
Immunology
Dang-Nghiem Vo, Nicolas Leventoux, Mauricio Campos-Mora, Sandrine Gimenez, Pierre Corbeau, Martin Villalba
Summary: NK cells play a critical role in the antiviral immune response against HIV-1. Impaired NK cell activity is observed in HIV-1 patients, with alterations in NK cell subsets and the emergence of memory NK cells. NK cells can acquire CCR5 and CXCR4 through trogocytosis from T cells, but not CD4. HIV-1 patients exhibit distinct NK cell clusters, with increased expression of CXCR4 and CCR5 in viremic patients. Degranulation and trogocytosis may be linked in HIV-1 infection.
Article
Pharmacology & Pharmacy
Francois Noverraz, Baptiste Robin, Solene Passemard, Benedicte Fauvel, Jessy Presumey, Emilie Rigal, Alan Cookson, Joel Chopineau, Pierre Martineau, Martin Villalba, Christian Jorgensen, Anne Aubert-Pouessel, Marie Morille, Sandrine Gerber-Lemaire
Summary: The newly developed excipient C16TreSuc can stabilize antibodies and enable pulmonary immunotherapy for the treatment of COVID-19 and other lung diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Ana Antunes, Luis Alvarez-Vallina, Federico Bertoglio, Nicolas Bouquin, Stephanie Cornen, Francis Duffieux, Pierre Ferre, Raphaelle Gillet, Christian Jorgensen, Mark B. Leick, Bernard Maillere, Helene Negre, Mireia Pelegrin, Nicolas Poirier, Dietmar Reusch, Bruno Robert, Guy Serre, Alain Vicari, Martin Villalba, Christoph Volpers, Gavin Vuddamalay, Herve Watier, Thierry Wurch, Lennart Zabeau, Stefan Zielonka, Baolin Zhang, Alain Beck, Pierre Martineau
Summary: The annual Antibody Industrial Symposium brought together scientists from academia and industry to discuss new advancements in antibody engineering and cell-based therapies. The symposium also addressed production and intellectual property protection issues.
Article
Oncology
Michael Constantinides, Alexis Fayd'herbe De Maudave, Marie Potier-Cartereau, Mauricio Campos-Mora, Guillaume Cartron, Martin Villalba
Summary: CD20 monoclonal antibodies have a strong ability to quickly kill B cells using different mechanisms. However, the assessment of killing is difficult. This study shows that common cytometry protocols involving centrifugation can miss important parts of the killing effect of drugs. Alternative methods are proposed to assess fast target cell killing in vitro, avoiding centrifugation or based on survival comparison or counting beads, which are applicable to B cell lines and primary tumor cells.
Article
Biochemistry & Molecular Biology
Chantal Reina-Ortiz, M. Pilar Mozas, David Ovelleiro, Fei Gao, Martin Villalba, Alberto Anel
Summary: Therapies based on allogenic Natural Killer (NK) cells, specifically expanded and activated NK (eNK) cells, have shown significant cytotoxicity against hematological cancers. This study aims to understand the changes that occur in healthy NK cells during the expansion process in order to develop a more potent eNK-based therapy. MicroRNA (miRNA) expression analysis revealed changes in the expression of 64 miRNAs, highlighting the up-regulation of miRs-146a, -124, -34a, and -10a, which play a key role in cell survival, and the down-regulation of miRs-199a, -223, and -340, associated with increased NK cell cytotoxicity. Validation experiments confirmed the functional importance of these miRNAs and demonstrated the wide variety of changes in eNK cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Mauricio Campos-Mora, William Jacot, Genevieve Garcin, Marie-Lise Depondt, Michael Constantinides, Catherine Alexia, Martin Villalba
Summary: NK cells infiltrate tumor environment and eliminate tumor cells. They can perform trogocytosis by acquiring tumor antigens from solid cancer cells. The extent of trogocytosis depends on the target cell and the antigen.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Minna Sivonen, Katja A. Sirvio, Sara Wojciechowski, Anssi Kailaanmaki, Satu Kaipainen, Aubrey Bailey, Martin Villalba, Tuija Kekarainen
Summary: In this study, we aimed to identify the optimal activation and expansion protocol for cytotoxic NK cells against glioblastoma in vitro. The results showed that the ex vivo proliferation capacity and functionality of NK cells were affected by multiple factors, including the donor, composition of starting material, cytokine combination, and the activation protocol. These findings could be utilized for upscaling clinical NK cell manufacturing.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Nerea Allende-Vega, Joaquin Marco Brualla, Paolo Falvo, Catherine Alexia, Michael Constantinides, Alexis Fayd'herbe de Maudave, Lois Coenon, Delphine Gitenay, Giulia Mitola, Paul Massa, Stefania Orecchioni, Francesco Bertolini, Isabel Marzo, Alberto Anel, Martin Villalba
Summary: Solid tumor cells have altered metabolism that can protect them from cytotoxic lymphocytes, but metformin can increase tumor cell sensitivity to cytotoxic lymphocytes. Research shows that metformin induces expression of NKG2DL and ICAM-1, promoting binding between lymphocytes and tumor cells, and reducing the growth of human hematological tumor cells.
SCIENTIFIC REPORTS
(2022)