Article
Plant Sciences
Pawel Pasko, Pawel Zagrodzki, Krzysztof Okon, Ewelina Prochownik, Miroslaw Krosniak, Agnieszka Galanty
Summary: This study aimed to evaluate the effects of broccoli sprouts on thyroid cells and rats. The results showed that broccoli sprouts can decrease the viability of thyroid cancer cells and prevent inflammation. The study also confirmed the safety of broccoli sprouts, but further research is needed.
Article
Food Science & Technology
Andrea Mahn, Carmen Elena Perez, Victor Zambrano, Herna Barrientos
Summary: Blanching conditions were investigated to maximize sulforaphane content in broccoli sprouts. Higher temperatures and shorter immersion times were found to increase sulforaphane content. The optimum conditions were blanching at 61 degrees C for 4.8 min, resulting in a 3.3-fold increase in sulforaphane content compared to untreated sprouts. This process could contribute to the development of functional foods and nutraceuticals containing sulforaphane.
Article
Chemistry, Applied
Li Zhuang, Kexin Xu, Yinglian Zhu, Fengwu Wang, Junxia Xiao, Liping Guo
Summary: Under ZnSO4 stress, CaCl2 treatment promoted growth and reduced oxidative stress in broccoli sprouts, while verapamil treatment resulted in the highest levels of stress markers. Both exogenous and endogenous calcium played a role in enhancing glucoraphanin biosynthesis and sulforaphane formation, with verapamil showing an adverse effect on sulforaphane production due to increased ESP expression and activity.
Article
Food Science & Technology
Adriana Vanegas Torres, Nimrod Tish, Victor Rodov
Summary: This study aimed to enhance the nutritional value of broccoli sprouts by using hydrogen peroxide (H2O2) as an elicitor. Daily spraying with H2O2 increased the accumulation of glucosinolates in the sprouts, changed their morphology, and did not affect the yield. However, when applied to wild rocket plants, H2O2 caused leaf bleaching and a decline in phenolics content. The synthesis of glutathione prevailed over the formation of glucosinolates in the sprouts.
Article
Food Science & Technology
Jan Triska, Josef Balik, Milan Houska, Pavla Novotna, Martin Magner, Nadezda Vrchotova, Pavel Hic, Ladislav Jilek, Katerina Thorova, Petr Snurkovic, Ivo Soural
Summary: Sulforaphane, a unique compound known for its health benefits, can be increased in broccoli sprouts by controlling temperature and adding co-factors. These technological steps are important for preserving sulforaphane, but there are limitations in the amount that can be achieved. By focusing on the extraction process, researchers aim to increase the amount of sulforaphane in the final extract for potential use as a dietary supplement.
Article
Food Science & Technology
Jelena Bokic, Dubravka Skrobot, Jelena Tomic, Vanja Seregelj, Angel Abellan-Victorio, Diego A. Moreno, Nebojsa Ilic
Summary: The study investigated the use of broccoli sprouts powder (BsP) as a novel food ingredient to improve the nutritional and bioactive profile of conventional pasta. Results showed that incorporating BsP increased the levels of protein, lipids, and minerals in the pasta, while reducing carbohydrates. BsP also increased the content of glucosinolates, synapic acid derivatives, total phenolics, and antioxidant activity in uncooked pasta. Although cooking slightly decreased the content of sinapic acid derivatives and total phenolics, their levels were still significantly higher in enriched pasta samples. However, there was a significant loss of glucosinolates during pasta cooking. The textural analysis showed that uncooked enriched pasta had lower flexibility and breaking strength, while cooked enriched pasta was less firm compared to the control. Incorporating BsP also increased the bitterness of the pasta, but overall pasta quality and liking were not significantly affected.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
W. C. Rivero, Qingyang Wang, Deepti Salvi
Summary: This study investigated the effectiveness of plasma-activated water (PAW) as a sanitizer for sprout washing. The results showed that PAW and chlorine (Cl) both achieved significant reductions in microbial counts on sprouts, with no significant difference in sprout quality.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Biophysics
Lixue Ma, Yan He, Lijun Bai, Mo Li, Xiaofan Sui, Bingyang Liu, Baocheng Tian, Yanhua Liu, Qiang Fu
Summary: A high drug-loaded MLX injection was successfully developed using nanocrystals, which showed significant analgesic effects.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Microbiology
Johanna M. Holman, Louisa Colucci, Dorien Baudewyns, Joe Balkan, Timothy Hunt, Benjamin Hunt, Marissa Kinney, Lola Holcomb, Allesandra Stratigakis, Grace Chen, Peter L. Moses, Gary M. Mawe, Tao Zhang, Yanyan Li, Suzanne L. Ishaq
Summary: Dietary intervention with steamed broccoli sprouts can protect against dysbiosis and colitis induced by dextran sodium sulfate (DSS), providing a potential therapeutic option for inflammatory bowel diseases.
Article
Agriculture, Multidisciplinary
Cui Li, Shuhui Song, Yanan He, Siyu Han, Haijie Liu
Summary: A new type of electrolyzed water, CaCl2-HCl electrolyzed water, was found to enhance glucosinolate metabolism in broccoli sprouts by activating calcium signaling pathway. This research provides a theoretical basis for cultivating high-quality broccoli sprouts.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Chemistry, Applied
Noelia Castillejo, Lorena Martinez-Zamora, Perla A. Gomez, Giuseppina Pennisi, Andrea Crepaldi, Juan A. Fernandez, Francesco Orsini, Francisco Artes-Hernandez
Summary: Yellow LED lighting enhances the accumulation of total phenolic and glucosinolate content, improving nutritional value, while Fluorescent light and Darkness reduce the glucosinolate content.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Shingo Kasamatsu, Takuma Owaki, Somei Komae, Ayaka Kinno, Tomoaki Ida, Takaaki Akaike, Hideshi Ihara
Summary: The consumption of broccoli has been linked to a reduced risk of various diseases, and it is rich in phytochemicals such as glucosinolates and isothiocyanates. This study focused on the endogenous polysulfide content of broccoli and the effects of germination on the polysulfide content and composition. The results showed that the polysulfide content significantly increased during germination, and cysteine hydropersulfide and cysteine hydrotrisulfide were the main organic polysulfide metabolites. Additionally, novel sulforaphane derivatives conjugated with polysulfides were produced in the broccoli sprouts and exhibited strong antioxidant properties. These findings have important implications for understanding the health-promoting effects of broccoli sprouts.
Article
Agronomy
Li Yang, Dimitrios Fanourakis, Georgios Tsaniklidis, Kun Li, Qichang Yang, Tao Li
Summary: Broccoli sprouts are rich in health-promoting bioactive compounds, and their content is influenced by both cultivation light quality and temperature. Higher temperature and blue light often enhance growth and compound production. In general, the content of bioactive compounds is higher in cotyledon compared to hypocotyl.
Article
Food Science & Technology
Li Zhuang, Guoqing Huang, Xiaodan Li, Junxia Xiao, Liping Guo
Summary: This study investigated the effects of various LED lights on the biochemical parameters and nutrition content of broccoli sprouts. Different colors of LED lights had different effects on the growth and nutrition content of the sprouts, with blue LED lights resulting in the highest levels of ascorbic acid and anthocyanins, and green LED lights resulting in the highest levels of ATP, ADP, and AMP.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Nutrition & Dietetics
Xiao Men, Xionggao Han, Se-Jeong Lee, Geon Oh, Keun-Tae Park, Jong-Kwon Han, Sun-Il Choi, Ok-Hwan Lee
Summary: It was found that broccoli sprout powder, mustard seed powder, and sulforaphane-rich mixture powder have anti-obesogenic effects by inhibiting adipocyte differentiation and adipogenesis, and by increasing the expression of proteins related to lipid metabolism and lipolysis.
Review
Chemistry, Applied
Francisco J. Salar, Raul Dominguez-Perles, Cristina Garcia-Viguera, Pablo S. Fernandez
Summary: This article critically reviews the main non-thermal processing alternatives for non-alcoholic fruit and vegetable-based beverages, analyzing their advantages and downsides compared to conventional thermal treatments. It provides insights into their practical application and suitability for the industry.
FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Diego Hernandez-Prieto, Pablo S. S. Fernandez, Vicente Agullo, Cristina Garcia-Viguera, Jose A. Egea
Summary: The present study examines the impact of a beverage containing citrus and maqui with various sweeteners on male and female consumers. The beverages were developed and tested as a source of polyphenols in an earlier study. Plasma samples were collected before and after two months of daily consumption, and metabolomics techniques were used to measure bioactive-compound levels. Advanced versions of ANOVA and clustering analysis were employed to determine the effects of sex and sweetener factors on these compounds. Machine learning techniques were also applied to improve the results. The findings demonstrate sex-specific regulation of certain compounds, such as caffeic acid and 3,4-dihydroxyphenylacetic acid for men, and trans ferulic acid (TFA) or naringenin glucuronide for women. Sweeteners, such as stevia for women or sucrose for men, were also observed to have an impact on the regulation of these compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Paula Garcia-Ibanez, Maria A. Nunez-Sanchez, Alba Oliva-Bolarin, Maria A. Martinez-Sanchez, Bruno Ramos-Molina, Antonio J. Ruiz-Alcaraz, Diego A. Moreno
Summary: Digestates from red cabbage sprouts and red radish sprouts, which are derived from cruciferous vegetables, have anti-inflammatory effects and could be used as dietary coadjuvants in the treatment of chronic inflammation. These findings were observed in an in vitro model, where the bioaccessible compounds from the sprouts significantly decreased the production of pro-inflammatory cytokines in stimulated cells.
Article
Food Science & Technology
Paola Sanchez-Bravo, Angel Abellan, Pedro J. Zapata, Cristina Garcia-Viguera, Raul Dominguez-Perles, Maria Jose Gimenez
Summary: Broccoli has been used to develop healthy beverages by supplementing craft beers with broccoli sprouts and powder, which are rich in bioactive sulforaphane (SFN). The SFN concentrations were high in the supplemented beers (5.00 and 2.54 mg/L for sprouts and powder, respectively) prior to bottling, and decreased by >50% after bottling. After 150 storage days, the SFN concentrations were 1.08 and 0.30 mg/L for sprouts and powder, respectively. Sensory analysis showed positive attributes in beers developed using sprouts, but also negative attributes associated with broccoli-based beer, such as cooked vegetables, alcoholic, and earthy leather flavors. Overall, the addition of fresh broccoli sprouts is more suitable for enhancing SFN content and sensory features in beer, providing a functional alternative appreciated by consumers.
Article
Biochemistry & Molecular Biology
Antonio Costa-Perez, Sonia Medina, Paola Sanchez-Bravo, Raul Dominguez-Perles, Cristina Garcia-Viguera
Summary: The by-products of grape winemaking process, such as grape stems, pomace, and wine lees, contain a diverse range of phytochemical compounds, notably polyphenols, which have health benefits. However, these by-products have negative impacts on the sustainability of the agro-food industry and the local environment. This study compares the polyphenolic profiles of these three residues and provides new knowledge on the role of yeast and lactic acid bacteria metabolism in diversifying the phenolic composition. The results show that grape stems have the highest diversity of polyphenols, followed closely by wine lees. Yeasts and LAB may play a key role in transforming phenolic compounds, resulting in new molecules with specific bioactivity features and improving the biological potential of these underexploited residues.
Article
Materials Science, Multidisciplinary
Erika Paulsen, Diego A. Moreno, Domingo Martinez-Romero, Cristina Garcia-Viguera
Summary: The impact of perforation-mediated modified atmosphere packaging (PM-MAP) on broccoli florets was studied at different temperature scenarios. PM-MAP slightly altered the composition of O-2 and CO2, preserving the external quality and bioactive compounds of florets for 21 days, even at 7 degrees C. The level of perforation affected the evolution of firmness and glucosinolate content, especially with increasing temperature. Therefore, the perforation level should be considered in the design of packaging for fresh-cut products.
Article
Agronomy
Angelo Signore, Fabio Amoruso, Victor M. Gallegos-Cedillo, Perla A. Gomez, Jesus Ochoa, Catalina Egea-Gilabert, Antonio Costa-Perez, Raul Dominguez-Perles, Diego A. Moreno, Jose Antonio Pascual, Juan A. Fernandez
Summary: By using agro-industrial compost as a substitute for peat, the growth of rocket salad can be improved without negatively affecting yield and dry weight percentage. This compost can also enhance the concentration of phytochemicals and vitamin C in the plants.
Article
Biochemistry & Molecular Biology
Diego Hernandez-Prieto, Alberto Garre, Vicente Agullo, Cristina A. Garcia-Viguera, Jose A. Egea
Summary: Metabolic diseases have been linked to excessive consumption of high-sugar content beverages. To address this, there has been an increased demand for plant-based formulations with health-promoting properties. A longitudinal trial with 140 volunteers was conducted to assess the bioavailability of (poly)phenols in a maqui-citrus beverage, and it was discovered that the sex of the volunteer and the type of sweetener added to the beverage had an impact on the bioavailability of the (poly)phenol metabolites. Clustering analysis also revealed patterns in the distribution of metabolites based on sex and/or sweeteners. These findings highlight the potential of stevia as a bioavailability enhancer and demonstrate that sex plays a role in the metabolism of (poly)phenols.
Article
Food Science & Technology
Nieves Baenas, Cristina Garcia-Viguera, Raul Dominguez-Perles, Sonia Medina
Summary: This study aimed to identify and quantify tryptophan, serotonin, and melatonin in winery by-products and evaluate their antioxidant capacity. The results showed that grape stems had the highest content of tryptophan and antioxidant capacity, while grape pomace contained higher levels of serotonin and melatonin. These findings suggest that winery by-products can be valuable sources of tryptophan, serotonin, and melatonin, with tryptophan being the most important contributor to the antioxidant capacity.
Article
Immunology
Omar Guadarrama-Enriquez, Gabriel Fernando Moreno-Perez, Maria Eva Gonzalez-Trujano, Guadalupe Esther Angeles-Lopez, Rosa Ventura-Martinez, Irene Diaz-Reval, Agustina Cano-Martinez, Francisco Pellicer, Nieves Baenas, Diego A. Moreno, Cristina Garcia-Viguera
Summary: This study investigates the analgesic and anti-inflammatory activities of an aqueous extract of Brassica oleracea var. italica sprouts (AEBS) and its active metabolite sulforaphane (SFN). The results demonstrate the potential of AEBS and SFN as natural products for the treatment of pain and inflammation.
INFLAMMOPHARMACOLOGY
(2023)
Review
Nutrition & Dietetics
Antonio Costa-Perez, Vanesa Nunez-Gomez, Nieves Baenas, Giuseppe Di Pede, Mariem Achour, Claudine Manach, Pedro Mena, Daniele Del Rio, Cristina Garcia-Viguera, Diego A. Moreno, Raul Dominguez-Perles
Summary: This systematic review summarizes the findings of human studies on the metabolism and bioavailability of glucosinolates (GSL) and isothiocyanates (ITC), providing a comprehensive analysis for future research and consultation on this less studied area of GSL for food and health.
Article
Biochemistry & Molecular Biology
Davide Risso, Gillian Dunngalvin, Sameer Saxena, Andrea Doolan, Lisa Spence, Kavita Karnik
Summary: This study investigated the gastrointestinal tolerance of low-calorie sugar D-allulose in young children and found that it was well tolerated. It can be considered as a candidate for replacing added sugars with lower caloric content in commercially produced goods.
Article
Biochemistry & Molecular Biology
Xinying Suo, Anna Baggio, Nicoletta Pellegrini, Silvia Vincenzetti, Elena Vittadini
Summary: This study investigated the effects of shape, gluten, and structural breakdown on the starch digestibility and glycemic index of gluten-containing and gluten-free pasta. The results showed that mastication effort, shape, and gluten content had significant impacts on starch digestion and glycemic index, with gluten being the major factor affecting the amount of digested starch.
Article
Biochemistry & Molecular Biology
Xinnuo Hu, Yanjun Yang, Cuihua Chang, Junhua Li, Yujie Su, Luping Gu
Summary: Fish collagen was enzymatically hydrolyzed using alkaline, papain, and ginger protease. The addition of ginger protease increased the degree of hydrolysis and resulted in changes in the amino acid composition and molecular weight distribution of the peptides. Combined hydrolysis with ginger protease improved the ACE inhibitory activity of the sample but affected fibroblast proliferation activity.
Article
Biochemistry & Molecular Biology
Tong Nie, Xin Wang, Aqun Li, Anshan Shan, Jun Ma
Summary: This study investigated the mechanism by which hesperidin prevents nonalcoholic fatty liver disease (NAFLD) by modulating fatty acid beta oxidation. The results suggest that hesperidin promotes fatty acid beta oxidation by activating SIRT1/PGC1 alpha, thus improving NAFLD.
Article
Biochemistry & Molecular Biology
Li Li, Jing Zhao, Jinxue Wang, Qianqian Xiong, Xuechun Lin, Xiaolei Guo, Fan Peng, Wangqun Liang, Xuezhi Zuo, Chenjiang Ying
Summary: The daily administration of 10 g d-1 inulin-type fructans significantly reduced the circulating arsenic retention in end-stage renal disease by increasing the arsenic clearance rate through urine and dialysis. However, it was found to be ineffective for other trace elements.
Article
Biochemistry & Molecular Biology
Chuanchuan Wang, Weixuan Chen, Yishan Jiang, Xiao Xiao, Qianhui Zou, Jiarui Liang, Yu Zhao, Qianxu Wang, Tian Yuan, Rui Guo, Xuebo Liu, Zhigang Liu
Summary: Autism Spectrum Disorder (ASD) symptoms may be improved through modulation of gut microbiota. This study investigated the effects of a synbiotic treatment on an ASD-like mouse model, and found that it rectified social impairments, attenuated inflammatory cytokine expressions, protected gut barrier integrity, and altered gut microbiota composition. The synbiotic treatment elevated beneficial metabolites and upregulated genes associated with their synthesis. Overall, the synbiotic combination mitigated ASD-related social impairments through regulation of the gut-brain axis.
Review
Biochemistry & Molecular Biology
Chunli Kong, Caiping Duan, Yixuan Zhang, Yiying Wang, Zheng Yan, Sumei Zhou
Summary: This review critically discusses the physicochemical properties of bioactive molecules in kidney beans and focuses on the current knowledge of non-starch polysaccharide (NSP) structure and function on intestinal health and metabolism. It also provides suggestions for further investigation and development of NSPs as novel plant carbohydrate resources.
Article
Biochemistry & Molecular Biology
Fei Shen, Qianqian Wang, Sami Ullah, Ya Pan, Minjie Zhao, Jing Wang, Ming Chen, Fengqin Feng, Hao Zhong
Summary: This study investigated the peristalsis-promoting potential of Ligilactobacillus acidipiscis YJ5 and its underlying molecular mechanism in relieving constipation. The results showed that L. acidipiscis YJ5 intervention increased intestinal peristalsis in zebrafish and mouse models, reduced colonic aquaporin expression, repaired intestinal morphological damage, enhanced the colonic mucosal barrier, and reversed the gut microbiota structure. The positive effects of L. acidipiscis YJ5 were attributed to its metabolites such as malic acid and heliangin.
Article
Biochemistry & Molecular Biology
Zhuo Qu, Jiahui Tian, Jiachen Sun, Ying Shi, Jianqiang Yu, Wannian Zhang, Chunlin Zhuang
Summary: Smoking is the main risk factor for lung cancer, but garlic oil compound DATS shows potential as a chemopreventive agent. DATS can inhibit the carcinogenic effects of tobacco and regulate gut microbiota, as well as activate the PPAR-gamma pathway to suppress tumor growth.
Article
Biochemistry & Molecular Biology
Yanli Zhang, Jielun Hu, Yadong Zhong, Shuai Liu, Liandi Liu, Xinyi Mu, Chunhua Chen, Shenji Yang, Guohao Li, Duoduo Zhang, Xinru Huang, Jinrui Yang, Xiaojun Huang, Shuigen Bian, Shaoping Nie
Summary: The study found that different ratios of insoluble and soluble dietary fiber have distinct effects on gut microbiota and serum metabolites. Increasing the ratio of insoluble dietary fiber increases the relative abundance and acid production of specific bacterial genera, while increasing the ratio of soluble dietary fiber increases the diversity and relative abundance of other bacterial genera and regulates metabolic pathways.
Article
Biochemistry & Molecular Biology
Enhui Tang, Tong Hu, Zhaokang Jiang, Xiaojun Shen, Huan Lin, Haiyan Xian, Xinlan Wu
Summary: Isoquercitrin alleviates lipopolysaccharide-induced intestinal mucosal barrier damage in mice by inhibiting the TLR4/MyD88/NF-kappa B signaling pathway and modulating the intestinal flora.
Article
Biochemistry & Molecular Biology
Nesma A. Abd Elrazik, Sahar A. Helmy
Summary: This study found that betanin, the major pigment in red beetroot, has a protective effect against bleomycin-induced pulmonary fibrosis in rats. The protective effect may be attributed to the attenuation of the NLRP3/IL-1 beta/TGF-beta 1 signaling pathway and the suppression of the EMT process.
Article
Biochemistry & Molecular Biology
Sha Li, Longchen Shang, Yuanyuan Chen, Rong Song, Jing Li, Bin Li
Summary: A new form of konjac fiber was prepared that could instantly expand in contact with digestive fluid, inhibiting the digestion of food. The digestion regulation ability of the fiber was influenced by the freezing temperature used during preparation, with the fiber prepared at -20 degrees C showing the best performance in delaying gastric emptying and preventing intestinal starch hydrolysis.
Article
Biochemistry & Molecular Biology
Xian Xia, Jiangdong Li, Rui Liang, Yi Li, Xiaojuan Ma, Ying Yang, Daniel Lozano-Ojalvo
Summary: This study aimed to reduce the allergenicity of ovomucoid through unfolding-assisted glycation. The results showed that maltose glycation effectively reduced the IgG-binding capacity of the allergen. Unfolded and glycated ovomucoid exhibited enhanced antioxidant activity and metal ion chelating ability.
