Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis

Understanding of the intestinal microbiota is crucial to enhance intestinal health and performance parameters in animals. A more exhaustive research of the intestinal microbiota of broilers could be of interest to implement appropriate intervention measures. The aim of the present study was to investigate the development of the predominant cecal microbiota in broilers that were fed a Lactobacillus salivarius DSPV 001P strain during a complete rearing using denaturing gradient gel electrophoresis (DGGE). Bacterial DNA from cecal samples of 24 broilers at different ages were amplified by PCR and analysed by DGGE. A total of 35 DGGE products were excised and sequenced. Distinctive differences in bacterial communities were observed in the caecum as broilers age. At early stages, identified bacteria within the caecum of broilers were predominantly Clostridium-related species. Also, some sequences had the closest match to the genus Escherichia/Shigella. Furthermore, the caecum was a reservoir rich in uncultured bacteria. The major difference observed in our study was an increase of potentially beneficial Lactobacillus at Day 45. These results may be attributed to modulation of the microbiota by the probiotic supplementation. The obtained data could be relevant for future studies related to the influence of the microbiota resulting from probiotic supplementation on the performance and the immunological parameters of broilers.