Article
Biochemistry & Molecular Biology
Alaleh Zati Zehni, Falk Batz, Aurelia Vattai, Till Kaltofen, Svenja Schrader, Sven-Niclas Jacob, Jan-Niclas Mumm, Helene Hildegard Heidegger, Nina Ditsch, Sven Mahner, Udo Jeschke, Theresa Vilsmaier
Summary: This study aimed to evaluate the prognostic value of steroid hormone receptor expression in breast cancer patients, focusing on the retinoid X receptor (RXR) and thyroid hormone receptors (THRs). The analysis showed significant correlations between RXR and THRs expression and survival in different breast cancer entities. The findings suggest the need for further investigation into molecular therapies targeting steroid hormone receptors in breast cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Daniel Zaienne, Laura Isigkeit, Julian A. Marschner, Silke Duensing-Kropp, Georg Hoefner, Daniel Merk
Summary: This study discovered a novel selective RXR agonist, valerenic acid, which exhibits unique subtype and homodimer specificity. Through structural modification of this natural product, an analogue with enhanced activity was identified.
Article
Biochemistry & Molecular Biology
Longying Jiang, Xueke Liu, Xujun Liang, Shuyan Dai, Hudie Wei, Ming Guo, Zhuchu Chen, Desheng Xiao, Yongheng Chen
Summary: This study aimed to investigate the mechanism of FXR binding to DNA elements and the heterodimer interactions in the FXR-DBD/RXR-DBD complex through structural, biochemical and bioinformatics analyses. Further biochemical assays showed that RAR, THR, and NR4A2 do not form heterodimers with RXR when bound to the IR1 sites, indicating that IR1 may be a unique binding site for the FXR/RXR heterodimer. These studies could provide a better understanding of dimerization specificity of nuclear receptors.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Peter W. Jurutka, Orsola di Martino, Sabeeha Reshi, Sanchita Mallick, Zhela L. Sabir, Lech J. P. Staniszewski, Ankedo Warda, Emma L. Maiorella, Ani Minasian, Jesse Davidson, Samir J. Ibrahim, San Raban, Dena Haddad, Madleen Khamisi, Stephanie L. Suban, Bradley J. Dawson, Riley Candia, Joseph W. Ziller, Ming-Yue Lee, Chang Liu, Wei Liu, Pamela A. Marshall, John S. Welch, Carl E. Wagner
Summary: The study demonstrated that modification of partial agonists like NEt-4IB and potent rexinoids like bexarotene can lead to compounds with improved RXR selectivity, decreased cross-signaling of other RXR-dependent nuclear receptors, increased LXRE-heterodimer selectivity, and enhanced anti-proliferative potential in leukemia cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Himanshu Tak, Sanjana Negi, Thumballi R. Ganapathi
Summary: Increasing crop productivity is crucial for global food supply, and closing stomata plays a key role in pathogen resistance and drought tolerance. Novel research has uncovered insights into stomatal closure networks that can enhance crop endurance.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemical Research Methods
Sara Yousefi Taemeh, Nima Dehdilani, Lena Goshayeshi, Sylvie Rival-Gervier, Jalil Mehrzad, Bertrand Pain, Hesam Dehghani
Summary: This study demonstrates the counteraction of negative regulatory elements in the Ovalbumin gene by CRISPR interference, and reveals the possibility of achieving estrogen-independent expression of the Ovalbumin gene through the deletion of steroid-dependent regulatory elements and negative regulatory elements.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Article
Oncology
Alaleh Zati Zehni, Falk Batz, Vincent Cavailles, Sophie Sixou, Till Kaltofen, Simon Keckstein, Helene Hildegard Heidegger, Nina Ditsch, Sven Mahner, Udo Jeschke, Theresa Vilsmaier
Summary: This study assessed the prognostic value of nuclear versus cytoplasmic RXR alpha expression in breast cancer, showing that the subcellular localization of RXR alpha may have different impacts on tumorigenesis and prognosis. Cytoplasmic RXR alpha expression was associated with a more aggressive disease course, while nuclear RXR alpha expression appeared to be a protective factor, indicating potential implications for targeted tumor therapy.
Review
Pediatrics
Juan F. Garcia Rivas, Robin D. Clugston
Summary: Congenital diaphragmatic hernia (CDH) is a severe birth defect with high mortality and long-term morbidity rates. The Retinoid Hypothesis, proposed by Greer et al. in 2003, has been supported by research conducted in the past 20 years. As our understanding of the role of retinoid signaling in diaphragm development improves, it holds promise for improving CDH outcomes.
PEDIATRIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Tao Lin, Yuechan Chen, Yanling Zhang, Yaoyao Li, Lingyu Gao, Zhiwei Zhang
Summary: The study investigated the transcription regulation of KLF7 in chicken adipose tissue, with findings showing that the 5'-flanking sequence of KLF7 has significant promoter activity. GATA2 enhanced promoter activity, while GATA3 inhibited it, indicating that GATA2 and GATA3 play important roles in regulating KLF7 expression.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2021)
Article
Endocrinology & Metabolism
Xiaowen Chen, Xin Hou, Hao Yang, Hao Liu, Jun Wang, Chenghui Wang
Summary: This study investigated the molecular function and interplay of EcR and RXR during the molting process of Chinese mitten crab. The expression level of RXR was found to be more stable and higher than EcR throughout the molting process. CO-IP and luciferase reporter assay experiments confirmed the molecular interaction between EcR and RXR, which significantly induced the transcription of the essential gene E75 in the ecdysteroid signaling pathway. These findings provide a theoretical basis for the endocrine control of molting in E. sinensis, as well as novel insights into the molecular mechanism of molting in crustaceans mediated by the ecdysteroid signaling pathway.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Iuliia Dorofeeva, Assylbek Zhylkibayev, Irina V. Saltykova, Venkatram Atigadda, Bibek Adhikari, Oleg S. Gorbatyuk, Maria B. Grant, Marina S. Gorbatyuk
Summary: The RXR agonist UAB126 has shown therapeutic potential for treating obesity and diabetic retinopathy. In the study, UAB126 improved metabolic function and glucose uptake in diabetic macrophages, reduced hyperglycemia, preserved retinal function, enhanced AMPK activity, and increased the expression of genes associated with lipid metabolism.
Review
Biochemical Research Methods
Pierre Germain, Natacha Rochel, William Bourguet
Summary: Retinoids, including vitamin A and its metabolites, play critical roles in regulating essential biological processes by controlling gene expression through binding to nuclear transcription factors. Heterodimers formed by retinoic acid receptors and retinoid X receptors mediate the pleiotropic effects of retinoids by recruiting co-regulatory complexes to target gene promoters.
EXPLORING NUCLEAR RECEPTORS
(2021)
Article
Cell Biology
Junhui Yu, Kui Yang, Jianbao Zheng, Pengwei Zhao, Jie Xia, Xuejun Sun, Wei Zhao
Summary: Our study reveals the regulatory mechanism of EZH2-mediated FXR silencing in colorectal tumorigenesis. We demonstrate that the combination of FXR agonist OCA and EZH2 inhibitor GSK126 exhibits synergistic inhibition effects on colon cancer cells by suppressing clonogenic growth, invasion, and promoting apoptosis. Mechanistically, the drug combination accelerates FXR nuclear location and upregulates CDX2 expression. Depletion of CDX2 attenuates the synergistic effects of the drug combination.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Jiaxin Liu, Yimin Li, Yaqi Gan, Qing Xiao, Ruotong Tian, Guang Shu, Gang Yin
Summary: The dysregulation of transcription factors is associated with malignant growth and colorectal cancer development. By analyzing differential transcription factors in colorectal cancer patients and establishing a genetic risk scoring model, ZNF26 was identified as a potential novel biomarker for diagnosis and prognostic evaluation of colorectal cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Subir Kumar Juin, Sathnur Pushpakumar, Utpal Sen
Summary: The study found that in diabetic kidney, PPAR gamma expression was decreased, while RXR alpha, RXR beta, and RAR gamma 1 expression was increased, along with upregulations of PAI-1, MMP-9, and MMP-13. Additionally, collagen IV, fibronectin, and laminin expression were increased, while elastin expression was decreased in the diabetic kidney.
