Article
Multidisciplinary Sciences
Andrea Piserchio, Kimberly J. Long, Luke S. Browning, Amanda L. Bohanon, Eta A. Isiorho, Kevin N. Dalby, Ranajeet Ghose
Summary: Protein translation, a high-energy process, requires robust regulation under energy-deprived conditions. The eEF-2K plays a crucial role in this regulation by suppressing translational elongation through phosphorylation of eEF-2. It has been suggested that eEF-2K's response to energy levels is mediated by AMPK and the mTOR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Medicinal
Zhengnian Li, Ryosuke Ishida, Yan Liu, Jinhua Wang, Yina Li, Yang Gao, Jie Jiang, Jianwei Che, Jason M. Sheltzer, Matthew B. Robers, Tinghu Zhang, Kenneth D. Westover, Behnam Nabet, Nathanael S. Gray
Summary: This study describes a medicinal chemistry campaign to identify a CDK11 inhibitor. Through structure-guided medicinal chemistry modifications, a CDK11 probe ZNL-05-044 with improved selectivity was identified, which can arrest cell cycle and impact CDK11-dependent gene splicing in cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Oncology
Na Ly Tran, Hyerim Kim, Cheol-Hee Shin, Eun Ko, Seung Ja Oh
Summary: In this study, we used an AI-driven screening strategy to discover a novel anticancer medication that targets STK33 and induces cancer cell apoptosis and cell cycle arrest at the S phase.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Qingxi Zhang, Yin Huang, Anbiao Wu, Qingrui Duan, Peikum He, Haifeng Huang, Yuyuan Gao, Kun Nie, Qicai Liu, Lijuan Wang
Summary: We identified the pathogenic role of calcium/calmodulin-dependent serine protein kinase in Parkinson's disease, showing that it phosphorylated alpha-synuclein to activate mitochondrial calcium uniporter and increase mitochondrial calcium influx, leading to mitochondrial damage. Our findings highlight the therapeutic potential of targeting calcium/calmodulin-dependent serine protein kinase in Parkinson's disease treatment.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Soghra Bagheri, Mahdie Rahban, Fatemeh Bostanian, Fatemeh Esmaeilzadeh, Arash Bagherabadi, Samaneh Zolghadri, Agata Stanek
Summary: Prostate cancer, when it spreads to the bone, is known as metastatic bone cancer. Currently, effective treatment options are still lacking for advanced/metastatic PC. However, understanding the molecular events that contribute to PC progression provides hope for the development of new treatment strategies. Protein kinases play crucial roles in the growth, proliferation, and metastases of prostatic tumors, making combinatorial therapy with PK inhibitors a potential treatment approach. Additionally, epigenetic pathways are also involved in PC, suggesting another potential combination treatment strategy.
Article
Biochemistry & Molecular Biology
Shuhang Dai, Elisa Venturini, Saveg Yadav, Xiaoxuan Lin, Dylan Clapp, Martin Steckiewicz, Angela M. Gocher-Demske, Arthur M. Edelman, D. Grahame Hardie
Summary: This study reveals the important role of CaMKK2 in mediating EGFR signaling in cancer cells, with regulation mechanisms involving both calcium-dependent and calcium-independent pathways. Additionally, CaMKK2 regulates transcription of key genes, impacting cell survival and proliferation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Sofia Beghi, Malgorzata Furmanik, Armand Jaminon, Rogier Veltrop, Nikolas Rapp, Kanin Wichapong, Elham Bidar, Annamaria Buschini, Leon J. Schurgers
Summary: Cardiovascular disease remains a major cause of death worldwide, despite advancements in medication and preventive measures. Calcium plays a vital role in the cardiovascular system, regulating events from action potentials to muscle contraction. Calcium calmodulin pathways are involved in the regulation of cardiac functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mahmoud I. Khalil, Heba M. Ismail, Ganna Panasyuk, Anna Bdzhola, Valeriy Filonenko, Ivan Gout, Olivier E. Pardo
Summary: Ribosomal S6 kinases (S6Ks) play crucial roles in cell growth, homeostasis, and survival, and dysregulation of these kinases is implicated in various malignancies. While S6K1 has been extensively studied, the involvement of S6K2 in cancer progression has been overlooked. This study reveals that p54-S6K2 undergoes asymmetric dimethylation at Arg-475 and Arg-477, mediated by methyltransferases PRMT1, PRMT3, and PRMT6. This methylation event leads to nuclear localization of S6K2, which is crucial for its pro-survival effects during starvation-induced cell death. These findings highlight a novel post-translational modification that regulates p54-S6K2 function, and its relevance in cancer progression where general arginine methylation is often elevated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Michal Marcinkowski, Tomas Pilzys, Damian Garbicz, Jan Piwowarski, Kaja Przygonska, Maria Winiewska-Szajewska, Karolina Ferenc, Oleksandr Skorobogatov, Jaroslaw Poznanski, Elzbieta Grzesiuk
Summary: FTO is an N-6-methyladenosine demethylase which can remove methyl groups from nucleic acids, forming complexes with other proteins. Research indicates an interaction between FTO and calmodulin (CaM), influencing calcium-binding loops in CaM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Chaorui Liu, Xiaonan Dong, Yuqi Xu, Qing Dong, Yuqi Wang, Yingping Gai, Xianling Ji
Summary: The response of mulberry to phytoplasma infection involves significant changes in both DNA methylation and gene expression, with the Mu-GsSRK gene playing a key role in enhancing plant resistance to pathogens. Overexpression of Mu-GsSRK not only increases resistance to phytoplasma but also upregulates the expression of pathogenesis-related proteins and other defense genes, contributing to enhanced disease resistance. The dynamic patterns of DNA methylation and gene functions in mulberry's response to phytoplasma infection were also discussed, highlighting the importance of DNA methylation in this process.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Akhmed Aslam, Jawwad Ahmad, Mohammed A. Baghdadi, Shakir Idris, Riyad Almaimani, Aiman Alsaegh, Mai Alhadrami, Bassem Refaat
Summary: This study found that vitamin D is superior to Paricalcitol in potentiating 5-FU cytotoxicity against colorectal cancer, possibly by upregulating several Ca2+-related molecules involved in tumor suppression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Plant Sciences
Lijuan Chen, Jiahui Xiao, You Li, Yuxiao Song, Jun Liu, Qi Zhou, Ting Sun, Hong-Bin Wang, Bing Liu
Summary: Plant diseases caused by fungal pathogens can be regulated through jasmonate response and chitin signaling, specifically by the Raf-like MAPKKKs STY8, STY17, and STY46, as discovered in this study. Interaction with MKK7, a MAPKK component, also plays a role in resistance to Botrytis cinerea and chitin signaling. The accumulation of MKK7 is negatively affected by the homologs of STY8, STY17, and STY46, which have opposite effects on defense against B. cinerea.
Article
Biochemistry & Molecular Biology
A. A. Zorina, G. V. Novikova, N. B. Gusev, A. V. Leusenko, D. A. Los, O. I. Klychnikov
Summary: Twelve potential serine-threonine protein kinases (STPKs) in Synechocystis sp. PCC 6803 genome were divided into two clusters: PKN2-type kinases and ABC1-type kinases. This study demonstrated that SpkH of Synechocystis is a true active serine protein kinase with substrate specificity similar to casein kinases and sensitivity to certain activity effectors.
Article
Biochemistry & Molecular Biology
Riku Kaneshige, Satomi Ohtsuka, Yuhei Harada, Issei Kawamata, Masaki Magari, Naoki Kanayama, Naoya Hatano, Hiroyuki Sakagami, Hiroshi Tokumitsu
Summary: This study reveals that the RP-domain is required for CaMKK isoforms to recognize and phosphorylate downstream kinases, potentially providing a promising target for specific CaMKK inhibitors.
Article
Fisheries
Chen Huang, Jie Du, Bairu Ji, Sinan Gong, Chao Geng, Yanyang Miao, Qingchun Shen, Wei Gu, Li Wang, Qingguo Meng
Summary: EsCaMKII is highly transcribed in various tissues of E. sinensis and its over-expression promotes apoptosis and enhances resistance to S. eriocheiris infection, while RNAi knockdown results in increased pathogen copy numbers and crab mortality.
FISH & SHELLFISH IMMUNOLOGY
(2022)