Article
Infectious Diseases
Francesca De Falco, Federica Corrado, Anna Cutarelli, Leonardo Leonardi, Sante Roperto
Summary: This study compared the levels of BPV DNA in bovine samples using ddPCR and qPCR, finding that ddPCR was more sensitive and accurate than qPCR, allowing the detection of more BPV genotypes.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Multidisciplinary Sciences
Drew F. K. Williamson, Sean R. N. Marris, Vanesa Rojas-Rudilla, Jacqueline L. Bruce, Cloud P. Paweletz, Geoffrey R. Oxnard, Lynette M. Sholl, Fei Dong
Summary: Activating mutations in EGFR can predict the effectiveness of tyrosine kinase inhibitors in treating advanced non-small cell lung cancer. This article presents a fast and accurate analytical method for detecting EGFR mutations.
Article
Oncology
Jin-Yi Han, Keun Soo Ahn, Tae-Seok Kim, Yong Hoon Kim, Kwang Bum Cho, Dong Woo Shin, Won-Ki Baek, Seong-Il Suh, Byeong-Churl Jang, Koo Jeong Kang
Summary: Utilization of cell free DNA from bile fluid shows promising potential for diagnosing and monitoring patients with biliary tract cancers, with mutational variants reliably detected and showing a strong positive correlation with tissue samples, suggesting bile as an effective biopsy fluid for ctDNA analysis.
Article
Biochemical Research Methods
Jarad Yost, Zachary Gagnon
Summary: In this study, a novel Faradaic reaction-free nucleic acid amplification method was proposed for microscale liquid samples, using a radio frequency alternating current electric field to induce Joule heating without producing electrolysis gaseous by-products. The approach demonstrated successful direct thermal amplification using two popular NAA biochemical reactions, offering substantial miniaturization of heating elements for microfluidic or miniaturized NAA reaction systems.
Article
Oncology
Hui Qi, Anwen Xiong, Lei Jiang, Hardy Van, June Xu, Jing Wu, Qiaosong Zheng, Fabrizio Minervini, Dinora Polanco Alonso, Yifu Yang, Liang Wu
Summary: This study demonstrates that blood dPCR may be a potential method to detect HER2 gene amplification in NSCLC patients, and the detection of HER2 amplification is correlated with patient survival rates.
TRANSLATIONAL LUNG CANCER RESEARCH
(2021)
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
Article
Oncology
Louise Raunkilde, Torben Frostrup Hansen, Rikke Fredslund Andersen, Birgitte Mayland Havelund, Caroline Brenner Thomsen, Lars Henrik Jensen
Summary: Although imaging is the gold standard in evaluating the treatment effect in metastatic colorectal cancer, this study found that a reduction in methylated circulating tumor DNA (ctDNA) after one treatment cycle is a better marker of progression-free survival than imaging. Methylated ctDNA can be an early indicator of treatment benefit and is a promising endpoint in clinical trials.
Article
Chemistry, Analytical
Zheng Lin Tan, Masato Yasuura, Yukichi Horiguchi, Hiroki Ashiba, Takashi Fukuda
Summary: This paper presents a new method called gel capsule-based digital PCR (gc-dPCR), which improves the stability of hydrogel capsules compared to droplets. This method enhances the sensitivity and stability of ddPCR, reduces its cost, and provides a more accurate quantification of low-concentration samples.
Article
Multidisciplinary Sciences
Nupur Garg, Upasana Sahu, Sudeshna Kar, Farhan J. Ahmad
Summary: This study introduces a fast, sensitive, and specific LAMP technique for early detection of leprosy, which showed complete concurrence with conventional techniques in blind clinical validation studies. Visual detection methods were also standardized for easy-to-read and rapid diagnosis, eliminating the lack of accuracy and sensitivity in skin smear tests and the need for expensive lab equipments and trained technicians.
SCIENTIFIC REPORTS
(2021)
Article
Biophysics
Yonghee Shin, Taejin Kwak, Keumrai Whang, Yuseung Jo, Jeong Ha Hwang, Inhyeok Hwang, Hyun Ji An, Youngwook Lim, Inhee Choi, Dongchoul Kim, Luke P. Lee, Taewook Kang
Summary: In this study, we utilize a hierarchically porous silica structure of single-celled algae to achieve bubble-free diatom polymerase chain reaction (PCR), solving the critical problem of air bubble trapping during PCR. The surface hydrophilicity and pore structure of the diatom allow for spontaneous loading of PCR solution without air bubble trapping, and a large pressure gradient between air bubbles and nanopores rapidly removes residual air bubbles. We successfully amplify DNA fragments and detect SARSCoV-2 with as low as 10 copies/μl using diatom PCR and a microfluidic device integrated with diatoms assembly. This work has the potential to revolutionize molecular diagnostics and provide new opportunities for diatoms in real-world applications.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Shun-ichi Matsuura, Tomoya Baba, Takuji Ikeda, Katsutoshi Yamamoto, Tatsuo Tsunoda, Aritomo Yamaguchi
Summary: The mesoporous silica-enhanced PCR technology allows highly precise and sensitive DNA amplification/detection from minimal amounts of nucleic acid, making it applicable for genetic testing involving small samples.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Chemistry, Analytical
Xinran Dong, Yuchen Huang, Dechen Jiang
Summary: A fluorescent polymerase chain reaction (PCR) nanokit was established for detecting specific DNA sequences in single living cells. This strategy involves sorting the DNA sequence in a cellular nucleus into a nanopipette, where it reacts with PCR kit components to complete a PCR amplification reaction. The presence of the target DNA sequence is validated through an increase in fluorescence, and the nanokit offers a general and highly sensitive approach for single cell analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jinchao Li, Jingmeng Cheng, Shanshan Li, Jie Jayne Wu, Junwei Li
Summary: In this research, we developed a chamber-based digital PCR (cdPCR) microfluidic device compatible with fluorescence imaging systems for detecting milk adulteration. The device enables digitalization of PCR reagents in microchambers, followed by DNA amplification through thermocycling. Fluorescence images of the microchambers are then analyzed to accurately quantify the target DNA and differentiate intentional adulteration from accidental contamination. The device was validated by authenticating camel milk samples, demonstrating its effectiveness in real biotechnical applications.
Article
Oncology
Yingyi Wang, Bao Jin, Na Zhou, Zhao Sun, Jiayi Li, Qiao Chen, Xiangan Wu, Yi Zhou, Yue Shi, Xin Lu, Xinting Sang, Yilei Mao, Shunda Du, Wenze Wang, Chunmei Bai
CURRENT CANCER DRUG TARGETS
(2020)
Article
Cell Biology
Hongsheng Liu, Yingzhi Qin, Na Zhou, Dongjie Ma, Yingyi Wang
Summary: ZNF280A plays a tumor-promoting role in LUAD, with a positive correlation with tumor grade, pathological stage, and lymphatic metastasis, and a negative relationship with patient survival. Its effects on cell proliferation, apoptosis, cell cycle, and cell migration may contribute to the development of LUAD.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Fei Tian, Shaohua Zhang, Chao Liu, Ziwei Han, Yuan Liu, Jinqi Deng, Yike Li, Xia Wu, Lili Cai, Lili Qin, Qinghua Chen, Yang Yuan, Yi Liu, Yulong Cong, Baoquan Ding, Zefei Jiang, Jiashu Sun
Summary: A thermophoretic aptasensor is utilized to profile cancer-associated proteins of extracellular vesicles (EVs) in patients' plasma, leading to the development of an EV signature capable of discriminating metastatic breast cancer, monitoring treatment response, and predicting patients' progression-free survival.
NATURE COMMUNICATIONS
(2021)
Article
Respiratory System
Yingyi Wang, Na Zhou, Rui Zhu, Xiaoyuan Li, Zhao Sun, Yang Gao, Wei Liu, Changting Meng, Yuping Ge, Chunmei Bai, Taisheng Li, Hongsheng Liu
Summary: The study found that untreated NSCLC patients have abnormal levels of peripheral blood immune cells, which can be used as an independent predictive biomarker for noninvasive early screening of NSCLC occurrence and progression.
BMC PULMONARY MEDICINE
(2021)
Article
Biochemical Research Methods
Lijuan Zhang, Yan Ju, Haixu Hu, Chunhui Ma, Yan Huang, Lili Gong, Yi Liu, Lihong Bian
Summary: This study established and validated a LAMP method for HPV detection, which has promising prospects and practical value in cervical cancer prevention, especially in undeveloped areas.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Immunology
Hui Tang, Ruixuan Geng, Xiuxiu Xu, Yingyi Wang, Jiaxin Zhou, Shulan Zhang, Lin Zhao, Mei Guan, Chunmei Bai
Summary: The study found that patients with preexisting ANA and anti-Ro52 antibodies did not have an increased risk of immune-related adverse events when receiving PD-1/PD-L1 inhibitors therapy, but patients positive for antithyroid antibodies should closely monitor immune-related thyroid dysfunction.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hui Tang, Tingting You, Zhao Sun, Chunmei Bai, Yingyi Wang
Summary: This study identified two HCC subtypes with distinct clinical outcomes, immune infiltration, and mutation characteristics. A prognostic model based on the ECM signature was developed, which may contribute to individualized prognostic prediction and aid in clinical decision-making.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Respiratory System
Hui Tang, Caixia Qiao, Yingyi Wang, Chunmei Bai
Summary: LPA is more frequently diagnosed in older people and women. Patients with LPA tend to have smaller tumor size, lower tumor grade and staging, which may indicate a favorable prognosis. The constructed nomograms accurately predict the long-term survival of LPA patients.
CANADIAN RESPIRATORY JOURNAL
(2022)
Article
Oncology
Jiayi Li, Qiao Chen, Lei Guo, Ji Li, Bao Jin, Xiangan Wu, Yue Shi, Haifeng Xu, Yongchang Zheng, Yingyi Wang, Shunda Du, Zhili Li, Xin Lu, Xinting Sang, Yilei Mao
Summary: This study quantitatively analyzed lipid molecules in tumors and adjacent tissues of intrahepatic cholangiocarcinoma (ICC) and established a diagnostic model to examine lipid changes with clinical classification. By identifying specific metabolites, the study successfully differentiated cancerous areas from adjacent normal areas. The research also found that certain lipid metabolites showed different trends between different clinical stages.
CANCER MANAGEMENT AND RESEARCH
(2022)
Article
Immunology
Na Zhou, Hui Tang, Shuangni Yu, Yi Lin, Yingyi Wang, Yuzhou Wang
Summary: PD-1/PD-L1 inhibitors show promising efficacy in patients with pulmonary lymphoepithelioma-like carcinoma (LELC), with higher PD-L1 expression being associated with tumor response, but its association with survival time remains to be determined.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Hui Tang, Caixia Qiao, Jun Lu, Yuejuan Cheng, Menghua Dai, Taiping Zhang, Junchao Guo, Yingyi Wang, Chunmei Bai
Summary: This study compared the efficacy and safety of adjuvant gemcitabine plus S-1 (GS) with S-1 monotherapy for pancreatic ductal adenocarcinoma (PDAC). The results showed that adjuvant S-1 monotherapy was noninferior to the GS regimen in disease-free survival and overall survival, with better tolerability.
Article
Oncology
Yuan Liu, Tao Lu, Mingming Yuan, Rongrong Chen, Junliang Lu, Hanping Wang, Zhiyong Wu, Yingyi Wang
Summary: This study found that PEAC has high genetic heterogeneity, and EGFR and ALK inhibitors can effectively treat PEAC. PD-L1 expression and KRAS mutation type may be predictive biomarkers for immunotherapy in PEAC. This study provided both theoretical basis and clinical evidence for PEAC.
Article
Immunology
Ruixuan Geng, Hui Tang, Tingting You, Xiuxiu Xu, Sijian Li, Zepeng Li, Yuan Liu, Wei Qiu, Na Zhou, Ningning Li, Yuping Ge, Fuping Guo, Yuhong Sun, Yingyi Wang, Taisheng Li, Chunmei Bai
Summary: PD-1/PD-L1 inhibitors can be effective in patients with high levels of CD8+CD28+ T cells, which can be used as a potential biomarker for predicting immunotherapy response.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Yanjie Han, Yuanyuan Xiong, Tao Lu, Rongrong Chen, Yuan Liu, Hui Tang, Ruixuan Geng, Yingyi Wang
Summary: HER2-mutant and HER2-amplified NSCLC have different molecular characteristics, and the genomic profile of HER2 NSCLC is dependent on tumor stage. Pyrotinib shows better therapeutic effects than afatinib in HER2 NSCLC patients. Resistance mechanisms to afatinib and pyrotinib, both HER2-dependent and -independent, have been identified.
FRONTIERS IN ONCOLOGY
(2023)
Article
Endocrinology & Metabolism
Lingge Wu, Yan Xu, Xiang Wang, Xinqi Cheng, Yuelun Zhang, Yingyi Wang, Xinrong Fan, Haitao Zhao, He Liu, Xiaofeng Chai, Li Zhang, Mengzhao Wang, Naishi Li, Hui Pan, Xiaolan Lian
Summary: This study retrospectively analyzed Chinese patients who received ICI therapy and found that approximately 44% of patients developed immune-related thyroid dysfunction. The most common thyroid irAE was overt hypothyroidism, followed by subclinical thyrotoxicosis and subclinical hypothyroidism. Thyroid dysfunction was associated with better progression-free survival and overall survival.