Article
Multidisciplinary Sciences
Haohao Wu, Charles Petitpre, Paula Fontanet, Anil Sharma, Carmelo Bellardita, Rolen M. Quadros, Paulo R. Jannig, Yiqiao Wang, J. Alexander Heimel, Kylie K. Y. Cheung, Simone Wanderoy, Yang Xuan, Konstantinos Meletis, Jorge Ruas, Channabasavaiah B. Gurumurthy, Ole Kiehn, Saida Hadjab, Francois Lallemend
Summary: The study identified three main types of proprioceptive neurons (Ia, Ib and II PNs) and found that they segregate into eight distinct subgroups. Importantly, Ia-PN subtypes are plastic, suggesting a role in adaptive proprioception during motor behavior.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yingqiao Wang, Raghav Garg, Jane E. Hartung, Adam Goad, Dipna A. Patel, Flavia Vitale, Michael S. Gold, Yury Gogotsi, Tzahi Cohen-Karni
Summary: Utilizing nanomaterial-assisted photothermal stimulation, Ti3C2Tx (MXene) demonstrates excellent potential as a candidate for remote, non-genetic, optical modulation of neuronal electrical activity with high spatiotemporal resolution. The material's photothermal response, low cytotoxicity, and ease of large-scale synthesis make it a promising tool for modulating electrophysiology at various scales.
Article
Engineering, Biomedical
Yingqiao Wang, Jane E. Hartung, Adam Goad, Matias A. Preisegger, Benjamin Chacon, Michael S. Gold, Yury Gogotsi, Tzahi Cohen-Karni
Summary: This study investigates the biosafety of Ti3C2Tx-based photothermal modulation. It finds that culturing neurons on Ti3C2Tx films and illuminating them with laser pulses do not cause damage to the cell membrane, induce cellular stress, or generate oxidative stress. These findings provide important insights for the clinical translation of photothermal modulation.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Gabriela I. Aparicio, Antonella Leon, Rocio Gutierrez Fuster, Baylen Ravenscraft, Paula V. Monje, Camila Scorticati
Summary: The peripheral nervous system can repair itself, and DRG neurons play a role in promoting axon regeneration after injury. The membrane glycoprotein GPM6a is present in DRG neurons and is required for neurite elongation. This suggests that GPM6a could contribute to axon regeneration in the peripheral nervous system.
Article
Medicine, Research & Experimental
Yiru Wang, Xinyi Wang, Renfei Qi, Ying Lu, Yu Tao, Dongsheng Jiang, Yufang Sun, Xinghong Jiang, Chunfeng Liu, Yuan Zhang, Jin Tao
Summary: The study reveals a novel mechanism by which IL-33/ST2 suppresses A-type K+ currents in sensory neurons, leading to increased neuronal excitability and pain sensitivity. This mechanism involves the suppression of the tumorigenicity 2 (ST2) receptor and the activation of a Syk-dependent p38 beta signaling pathway. Targeting IL-33/ST2-mediated p38 beta signaling may represent a therapeutic approach to ameliorate pain behaviors.
Article
Clinical Neurology
Ana Nascimento, Tiago F. Da Silva, Elisabete C. Fernandes, Liliana L. Luz, Fernando M. Mar, Boris Safronov, Monica M. Sousa
Summary: This study reveals the presence of axon initial segments (AIS) in sensory dorsal root ganglion (DRG) neurons and their role in spontaneous discharges in neuropathic pain. The AIS in myelinated DRG neurons contains sodium channels that are associated with spontaneous activity. Computer simulations suggest that the AIS plays a crucial role in initiating spontaneous discharges. The disruption of the AIS leads to mechanical allodynia in neuropathic pain, indicating its significance in chronic pain management.
Article
Cell Biology
Bin Pan, Di Guo, Li Jing, Ke Li, Xin Li, Gen Li, Xiao Gao, Zhi-Wen Li, Wei Zhao, Hu Feng, Meng-Han Cao
Summary: This study reveals the role of lncRNA Pvt1 in the repair of peripheral nerve injury and highlights it as a potential treatment target. The findings show that Pvt1 increases c-Jun expression through sponging miRNA-214, enhancing the proliferation and migration of Schwann cells, and improving the regeneration of injured sciatic nerves and locomotor function in mice.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Qianqian Chen, Yang Miao, Zhipeng Xu, Ruirui Zhang, Sheng Yi
Summary: Schwann cells play a crucial role in nerve regeneration and are commonly used in tissue engineering. Identifying Schwann cells with enhanced migration ability is important for improving recovery effects. This study compared Schwann cells from different anatomical locations and found that dorsal root ganglia (DRG) Schwann cells have faster migration speed than sciatic nerve (SN) Schwann cells.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Clinical Neurology
Ristiawan Muji Laksono, Taufiq Agus Siswagama, Fa'urinda Riam Prabu Nery, Walter van der Weegen, Willy Halim
Summary: Experimental study shows that 2 Hz PRF has less physiological impact on healthy neurons compared to 4 Hz, making it a preferred frequency for neuron-targeted therapy in clinical applications.
JOURNAL OF PAIN RESEARCH
(2023)
Editorial Material
Immunology
Peter L. Wang, Rafael S. Czepielewski, Gwendalyn J. Randolph
Summary: The nervous system plays crucial roles in maintaining tissue homeostasis and regulating inflammatory responses. Recent studies have shown that lymph nodes are innervated by unique peptidergic nociceptors, signaling to various cell types within the lymph nodes.
TRENDS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
David Diaz Vera, Jonathan R. Soucy, Audrey Lee, Ryan A. Koppes, Abigail N. Koppes
Summary: The study examined the effects of optogenetic light irradiation on rat nerve tissues, finding that 470 nm light reduced neurite outgrowth in neurons without causing cell death. Schwann cells were insensitive to light irradiation, with their functions remaining unaffected following stimulation. These findings highlight the importance of considering cell type-specific responses and stimulation parameters in optogenetic applications.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Neurosciences
Markus Leo, Linda-Isabell Schmitt, Andrea Kutritz, Christoph Kleinschnitz, Tim Hagenacker
Summary: Cisplatin has a significant impact on satellite glial cells (SGCs) and dorsal root ganglion (DRG) neurons, affecting the expression of various proteins and potentially contributing to the neurotoxic side effects. Furthermore, SGC-secreted factors play a role in modulating the excitability of sensory neurons, suggesting that targeting SGCs could be a potential strategy in managing chemotherapy-induced neuropathic pain.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Cell Biology
Samira Osman, Ayssar Tashtush, David E. Reed, Alan E. Lomax
Summary: The gut-brain axis has been a subject of recent attention, with new evidence suggesting that colonic microbes can impact brain function and behavior. However, there is limited knowledge about the innervation of the colon by vagal afferent neurons, a major component of the gut-brain axis. This study aimed to investigate the innervation of the colon by quantifying the number of vagal and spinal afferent neurons that innervate the colon, and determine if these neurons simultaneously innervate the proximal and distal colon. The results showed that both vagal and spinal afferent neurons innervate the colon, with a smaller portion of vagal neurons innervating the distal colon. Additionally, there was overlapping innervation between vagal and spinal afferent neurons that innervate both the proximal and distal colon.
CELL AND TISSUE RESEARCH
(2023)
Article
Orthopedics
D. Chao, H. Tran, Q. H. Hogan, B. Pan
Summary: GFS produces analgesia in MIA-OA rats at least in part by producing blockade of afferent inputs, possibly also by blocking efferent activity from the dorsal horn.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Review
Pharmacology & Pharmacy
Jose Torres-Perez, Jahanzaib Irfan, Muhammad Rizki Febrianto, Simone Di Giovanni, Istvan Nagy
Summary: The article discusses the role of histone post-translational modifications (PTMs) in influencing nociception and pain phenotypes following tissue injury, and the opportunities and challenges of targeting histone PTMs to relieve pain in clinically relevant tissue injuries.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2021)
Article
Materials Science, Biomaterials
Aditya Jindal, Kolos Molnar, Andrew McClain, Bruno Paiva dos Santos, Melissa Camassola, Judit E. Puskas
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2020)
Article
Biotechnology & Applied Microbiology
Bruno Paiva dos Santos, Bertrand Garbay, Mattia Pasqua, Elsa Chevron, Zoeisha S. Chinoy, Christophe Cullin, Katell Bathany, Sebastien Lecommandoux, Joelle Amedee, Hugo Oliveira, Elisabeth Garanger
JOURNAL OF BIOTECHNOLOGY
(2019)
Article
Engineering, Biomedical
Bruno Paiva dos Santos, Bertrand Garbay, Mathilde Fenelon, Marie Rosselin, Elisabeth Garanger, Sebastien Lecommandoux, Hugo Oliveira, Joelle Amedee
ACTA BIOMATERIALIA
(2019)
Article
Engineering, Biomedical
Leonardo Marasca Antonini, Tiago Lemos Menezes, Adilar Goncalves dos Santos, Antonio Shigueaki Takimi, Denis Jardim Villarinho, Bruno Paiva dos Santos, Melissa Camassola, Jossano Saldanha Marcuzzo, Celia de Fraga Malfatti
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2019)
Article
Engineering, Biomedical
Davit Hakobyan, Chantal Medina, Nathalie Dusserre, Marie-Laure Stachowicz, Charles Handschin, Jean-Christophe Fricain, Julie Guillermet-Guibert, Hugo Oliveira
Article
Cell Biology
Bianca M. de Souza, Michelle Rodrigues, Fernanda S. de Oliveira, Liana P. A. da Silva, Ana P. Boucas, Ciro P. Portinho, Bruno P. dos Santos, Melissa Camassola, Dagoberto Rocha, Simone Lysakowski, Juliano Martini, Cristiane B. Leitao, Nance B. Nardi, Andrea C. Bauer, Daisy Crispim
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2020)
Article
Cell Biology
Alice Leroux, Bruno Paiva dos Santos, Jacques Leng, Hugo Oliveira, Joelle Amedee
CELL COMMUNICATION AND SIGNALING
(2020)
Article
Engineering, Biomedical
Marie Cuvellier, Frederic Ezan, Hugo Oliveira, Sophie Rose, Jean-Christophe Fricain, Sophie Langouet, Vincent Legagneux, Georges Baffet
Summary: Bioprinting technology has demonstrated the ability to create complex liver models with defined and organized structures, showing long-term viability and functionality. This research lays the foundation for bioprinting complex multicellular models, providing new perspectives for studying fibrosis development and cellular communication control.
Article
Engineering, Biomedical
H. Oliveira, C. Medina, G. Labrunie, N. Dusserre, S. Catros, L. Magnan, C. Handschin, M. L. Stachowicz, J-C Fricain, N. L'Heureux
Summary: This study emphasizes the importance of creating a functional vascular system for the survival of bioengineered tissues. The authors experimented with a new biomaterial called cell-assembled extracellular matrix (CAM) to create a vascular system. They demonstrated successful production and maturation of capillary-like structures using CAM sheets, which were able to connect to the host circulatory system in a mouse model.
Article
Engineering, Biomedical
Camille Douillet, Marc Nicodeme, Loic Hermant, Vanessa Bergeron, Fabien Guillemot, Jean-Christophe Fricain, Hugo Oliveira, Mikael Garcia
Summary: Researchers have developed a model using bioprinting technology to replicate the dynamic remodeling of fibroblasts in vitro. By patterning fibroblasts and myofibroblasts on collagen gels, the researchers successfully simulated the matrix deformations and reorganizations generated by the cells. This method provides a new tool for studying skin tissue organization and repair processes.
Article
Engineering, Biomedical
Nicolas Touya, Mathilde Devun, Charles Handschin, Sophia Casenave, Naima Ahmed Omar, Alexandra Gaubert, Nathalie Dusserre, Hugo De Oliveira, Olivia Kerouredan, Raphael Devillard
Summary: Laser-assisted bioprinting (LAB) offers high resolution and reproducibility for bone regeneration. This study developed a tricalcium silicate-based solidifying ink for bone repair and demonstrated its cytocompatibility and potential to promote bone volume formation in vitro and in vivo. However, LAB techniques with this ink failed to achieve complete bone repair.
Article
Engineering, Biomedical
Marie Cuvellier, Sophie Rose, Frederic Ezan, Ulrich Jarry, Hugo de Oliveira, Arnaud Bruyere, Christophe Drieu La Rochelle, Vincent Legagneux, Sophie Langouet, Georges Baffet
Summary: In recent decades, the development of 3D in vitro cultures of primary human hepatocytes (PHHs) has become increasingly important for mimicking liver functions. This study utilized 3D bioprinting technology to print PHH in a GelMa matrix, resulting in the rapid organization of polarized hollow spheroids that remained viable for at least 28 days. The bioprinted PHH exhibited high differentiation and maintained liver-specific functions over time, including the activities of phase I and II biotransformation enzymes. The study also demonstrated the ability of the bioprinted structures to vascularize when implanted in mice. This model shows promise for generating human liver tissue and advancing biotechnological developments.
Article
Multidisciplinary Sciences
Vanessa P. A. Lemos, Michele Porto, Rafael Da S. Cezar, Bruno P. Dos Santos, Melissa R. De Souza, Juliana Da Silva, Nance B. Nardi, Melissa Camassola
Summary: Mesenchymal stem cells derived from adipose tissue and bone marrow display different characteristics in different passages. Adipose stem cells have stronger migration ability in the initial passage, while bone marrow stem cells have higher osteogenic potential in the early passages. Cells in advanced passages show higher proliferation and colony forming abilities, but also exhibit genetic damage.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Correction
Engineering, Biomedical
Davit Hakobyan, Chantal Medina, Nathalie Dusserre, Marie-Laure Stachowicz, Charles Handschin, Jean-Christophe Fricain, Julie Guillermet-Guibert, Hugo Oliveira