Article
Cell Biology
Frederik Paulussen, Chetan P. Kulkarni, Frank Stolz, Eveline Lescrinier, Stijn De Graeve, Suzan Lambin, Arnaud Marchand, Patrick Chaltin, Peter In't Veld, Joseph Mebis, Jan Tavernier, Patrick Van Dijck, Walter Luyten, Johan M. Thevelein
Summary: The presence of sugar in the gut induces the expression of SGLT1 and stimulates sugar absorption in intestinal epithelial cells. The beta 2-adrenergic receptor (beta(2)-AR) is strongly expressed and co-localized with SGLT1 on the enterocyte apical membrane, and it plays a role in stimulating glucose uptake from the gut by SGLT1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Adisorn Ratanayotha, Makoto Matsuda, Yukiko Kimura, Fumiko Takenaga, Tomoaki Mizuno, Md Israil Hossain, Shin-ichi Higashijima, Takafumi Kawai, Michio Ogasawara, Yasushi Okamura
Summary: The findings reveal the crucial role of Vsp protein in regulating endocytosis-dependent nutrient absorption in specialized enterocytes, and provide important insights into the physiological function of Vsp in animal species.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kacper Zbieralski, Donata Wawrzycka
Summary: Alpha-arrestins are a conserved family of proteins that selectively identify membrane proteins for ubiquitination and degradation, playing a crucial role in maintaining membrane protein homeostasis and global cellular metabolisms. Although the functions and regulation mechanisms of alpha-arrestins are not fully understood, their roles in redox homeostasis regulation, innate immune response regulation, and tumor suppression have been characterized in animal models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tatiana Sorkina, Mary Hongying Cheng, Tarique R. Bagalkot, Callen Wallace, Simon C. Watkins, Ivet Bahar, Alexander Sorkin
Summary: A series of small molecules have been discovered to stabilize trimers and induce high-order oligomers of DAT, ultimately promoting its endocytosis. The small molecule-induced DAT oligomerization and endocytosis are favored by the inward-facing DAT conformation and involve interactions of four hydrophobic residues at the interface between TM helices TM4 and TM9.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Arivarasu N. Anbazhagan, Shubha Priyamvada, Anoop Kumar, Dulari Jayawardena, Alip Borthakur, Ravinder K. Gill, Waddah A. Alrefai, Pradeep K. Dudeja, Seema Saksena
Summary: NHE-3 is a major apical membrane transporter involved in Na+ absorption in the intestine, with dysregulation implicated in diarrhea associated with gut inflammation and infections. Studies show that miR-326 and miR-330-5p play a role in regulating NHE-3 expression through posttranscriptional pathways.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yuqi Zhang, Yizeng Li, Keyata N. Thompson, Konstantin Stoletov, Qinling Yuan, Kaustav Bera, Se Jong Lee, Runchen Zhao, Alexander Kiepas, Yao Wang, Panagiotis Mistriotis, Selma A. Serra, John D. Lewis, Miguel A. Valverde, Stuart S. Martin, Sean X. Sun, Konstantinos Konstantopoulos
Summary: Cell migration plays a crucial role in various physiological processes, including cancer metastasis. This study reveals the polarization of NHE1 and SWELL1 at different regions of migrating cells, which is important for cell volume regulation and directional migration. The findings also highlight the physiological significance of the Osmotic Engine Model in cell migration and cancer metastasis.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Laszlo Dobson, Andras Zeke, Gabor E. Tusnady
Summary: This study utilized neural networks to classify transmembrane proteins on apical and basolateral membranes, aiding in understanding drug transport and its effects. The method provided may assist researchers in identifying or characterizing molecular networks regulating the distribution of transporters or receptors.
Article
Biochemistry & Molecular Biology
Yue Chen, Xiabing Gu, Yikai Zhang, Xianrui Zhang, Ce Zhang, Meng Liu, Shijin Sun, Ningzheng Dong, Qingyu Wu
Summary: Vitamin B12 is absorbed and reabsorbed through receptor-mediated endocytosis, with CD320 playing a key role in cellular uptake. This study explores the distribution and cellular expression of CD320 in mouse kidneys and intestines, revealing its involvement in vitamin B12 absorption in the small intestine and reabsorption in the kidney. Furthermore, a specific amino acid motif in CD320 is identified as crucial for its apical membrane targeting in both renal and intestinal epithelial cells, and this targeting is mediated by a Rab11a-dependent mechanism.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Keiko Maeda, Nicholas C. Zachos, Megan H. Orzalli, Stefanie S. Schmieder, Denis Chang, Katlynn Bugda Gwilt, Michele Doucet, Nicholas W. Baetz, Sun Lee, Sue E. Crawford, Mary K. Estes, Jonathan C. Kagan, Jerrold R. Turner, Wayne Lencer
Summary: Polarized epithelial cells form a crucial barrier against infection, but certain viruses and bacterial toxins can enter the cells through the apical membrane. The loss of the cell polarity gene PARD6B and aPKC leads to the degradation of apical endosome function, rendering the cells resistant to further infection.
CELL HOST & MICROBE
(2022)
Review
Biochemistry & Molecular Biology
Meng-Hsuan Wen, Xihong Xie, Pei-San Huang, Karen Yang, Tai-Yen Chen
Summary: Imbalanced copper homeostasis and perturbation of membrane trafficking are common symptoms associated with the pathogenesis of neurodegenerative and neurodevelopmental diseases. Studies suggest that membrane trafficking orchestrates both copper homeostasis and neural functions, and misregulated trafficking regulators may interact with copper dyshomeostasis to contribute to devastating pathogenesis in neurodegenerative diseases. Multiple unsolved questions and challenges exist in understanding how copper homeostasis is modulated through membrane trafficking.
Article
Cell Biology
Dandan Liu, Simona Paladino, Chiara Zurzolo, Stephanie Lebreton
Summary: Protein secretion is crucial for maintaining the balance of epithelial tissue and needs to be tightly regulated. Recent studies have shown that the calcium manganese pump SPCA1 and calcium-binding protein Cab45 play a role in regulating the secretion of certain soluble cargoes in nonpolarized cells. Our research focuses on the mechanism governing the polarized secretion of soluble cargoes in polarized epithelial cells, highlighting the critical role of a calcium-dependent Cab45 mechanism in regulating apical exocytosis.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Biochemistry & Molecular Biology
Markus M. Rinschen, Jennifer L. Harder, Madalina E. Carter-Timofte, Luis Zanon Rodriguez, Carmen Mirabelli, Fatih Demir, Naziia Kurmasheva, Suresh K. Ramakrishnan, Madlen Kunke, Yifan Tan, Anja Billing, Eileen Dahlke, Alexey A. Larionov, Wibke Bechtel-Walz, Ute Aukschun, Marlen Grabbe, Rikke Nielsen, Erik I. Christensen, Matthias Kretzler, Tobias B. Huber, Christiane E. Wobus, David Olagnier, Gary Siuzdak, Florian Grahammer, Franziska Theilig
Summary: This study reveals that lipid kinase VPS34 plays important roles in controlling metabolism, endocytosis, autophagy, and antiviral responses in proximal tubule cells in the kidney. The deficiency of VPS34 leads to alterations in the metabolome, membrane recycling, and fuel utilization, as well as an antiviral response. The findings provide insights into the function of VPS34 in kidney cells and its potential implications in diseases such as cancer and metabolic disorders.
Article
Biochemistry & Molecular Biology
Dolores Piniella, Elena Martinez-Blanco, David Bartolome-Martin, Ana B. Sanz-Martos, Francisco Zafra
Summary: This study identified novel potential partners for the dopamine transporter DAT and confirmed their association with DAT. Some of these proteins and lipid substances were shown to increase DAT activity, while inhibition of certain enzymes resulted in reduced functionality.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pavel Janos, Alessandra Magistrato
Summary: The secondary active Na-K-Cl cotransporter 1 (NKCC1) plays a significant role in regulating ion balance and is associated with various human diseases. This study predicts a potential binding mode of loop diuretic drugs to zebrafish and human NKCC1 orthologs. The drugs utilize the plasticity of ions in their binding process. The research provides insights for the development of more selective and potent NKCC1 inhibitors and highlights the importance of dynamical effects in ion-mediated drug binding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jean-Baptiste Brault, Sabine Bardin, Marusa Lampic, Jacopo A. Carpentieri, Laure Coquand, Maxime Penisson, Hugo Lachuer, Guiliana Soraya Victoria, Sarah Baloul, Fatima El Marjou, Gaelle Boncompain, Stephanie Miserey-Lenkei, Richard Belvindrah, Vincent Fraisier, Fiona Francis, Franck Perez, Bruno Goud, Alexandre D. Baffet
Summary: This study reveals that the delamination process of radial glial (RG) cells in the developing neocortex is regulated by the post-Golgi secretory pathway. The transport of RAB6+ vesicles towards the minus ends of microtubules depends on dynein, and the apical determinant Crumbs3 (CRB3) is also transported by dynein. Knockout of RAB6A/A' and RAB6B, as well as LIS1, impairs the apical localization of CRB3 and leads to delamination and ectopic division of aRG cells.
Article
Cell Biology
Andrew O'Neil, Christine P. Petersen, Eunyoung Choi, Amy C. Engevik, James R. Goldenring
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2017)
Article
Gastroenterology & Hepatology
Christine P. Petersen, Anne R. Meyer, Carlo De Salvo, Eunyoung Choi, Cameron Schlegel, Alec Petersen, Amy C. Engevik, Nripesh Prasad, Shawn E. Levy, R. Stokes Peebles, Theresa T. Pizarro, James R. Goldenring
Article
Gastroenterology & Hepatology
Amy C. Engevik, Izumi Kaji, Melinda A. Engevik, Anne R. Meyer, Victoria G. Weis, Anna Goldstein, Michael W. Hess, Thomas Muller, Hermann Koepsell, Pradeep K. Dudeja, Matthew Tyska, Lukas A. Huber, Mitchell D. Shub, Nadia Ameen, James R. Goldenring
Article
Cell Biology
Amy C. Engevik, Izumi Kaji, Meagan M. Postema, James J. Faust, Anne R. Meyer, Janice A. Williams, Gillian N. Fitz, Matthew J. Tyska, Jean M. Wilson, James R. Goldenring
JOURNAL OF CELL BIOLOGY
(2019)
Review
Physiology
Amy C. Engevik, Izumi Kaji, James R. Goldenring
PHYSIOLOGICAL REVIEWS
(2020)
Article
Gastroenterology & Hepatology
Amy C. Engevik, Alexander W. Coutts, Izumi Kaji, Paula Rodriguez, Felipe Ongaratto, Milena Saqui-Salces, Ramya Lekha Medida, Anne R. Meyer, Elena Kolobova, Melinda A. Engevik, Janice A. Williams, Mitchell D. Shub, Daniel F. Carlson, Tamene Melkamu, James R. Goldenring
Article
Multidisciplinary Sciences
Jimin Min, Paige N. Vega, Amy C. Engevik, Janice A. Williams, Qing Yang, Loraine M. Patterson, Alan J. Simmons, R. Jarrett Bliton, Joshua W. Betts, Ken S. Lau, Scott T. Magness, James R. Goldenring, Eunyoung Choi
NATURE COMMUNICATIONS
(2019)
Article
Microbiology
Melinda A. Engevik, Heather A. Danhof, Wenly Ruan, Amy C. Engevik, Alexandra L. Chang-Graham, Kristen A. Engevik, Zhongcheng Shi, Yanling Zhao, Colleen K. Brand, Evan S. Krystofiak, Susan Venable, Xinli Liu, Kendal D. Hirschi, Joseph M. Hyser, Jennifer K. Spinler, Robert A. Britton, James Versalovic
Summary: The study demonstrates that Fusobacterium nucleatum can promote intestinal inflammation by activating Toll-like receptor 4 and NF-KB, leading to disruption of colonic architecture, immune cell infiltration, and increased levels of proinflammatory cytokines. The findings suggest a potential mechanism by which F. nucleatum contributes to intestinal inflammation in the context of a depleted gut microbiome.
Article
Gastroenterology & Hepatology
Amy C. Engevik, Evan S. Krystofiak, Izumi Kaji, Anne R. Meyer, Victoria G. Weis, Anna Goldstein, Alexander W. Coutts, Tamene Melkamu, Milena Saqui-Salces, James R. Goldenring
Summary: The study investigates the role of Myosin Vb (MYO5B) in cell polarity and the formation of inclusions in microvillus inclusion disease (MVID). The results show that the altered localization of apical polarity proteins and tight junction proteins occurs after loss of Myo5b, both in mouse models and a swine model of MVID. This suggests a potential mechanism for tight junction protein accumulation in MVID patients.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Medicine, Research & Experimental
Izumi Kaji, Joseph T. Roland, Sudiksha Rathan-Kumar, Amy C. Engevik, Andreanna Burman, Anna E. Goldstein, Masahiko Watanabe, James R. Goldenring
Summary: Functional loss of MYO5B leads to various deficits in intestinal epithelial cell function and results in congenital diarrheal disorder, MVID. Loss of MYO5B reduces tuft cell and increases Paneth cell populations, along with expansion of the progenitor cell zone. Notch signaling pathway appears to be maintained in MYO5B-deficient intestine.
Article
Gastroenterology & Hepatology
Sarah A. Dooley, Kristen A. Engevik, Jessica Digrazia, Rachel Stubler, Izumi Kaji, Evan Krystofiak, Amy C. Engevik
Summary: Intestinal enterocytes have an elaborate apical membrane of actin-rich protrusions known as microvilli. The organization of microvilli is regulated by the intermicrovillar adhesion complex (IMAC), and this study discovered that Myosin 5b is responsible for the delivery of IMAC components to the apical membrane. Loss of Myosin 5b results in a disorganized brush border and failure of IMAC proteins to reach the distal tips of microvilli.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Melinda A. Engevik, Beatrice Herrmann, Wenly Ruan, Amy C. Engevik, Kristen A. Engevik, Faith Ihekweazu, Zhongcheng Shi, Berkley Luck, Alexandra L. Chang-Graham, Magdalena Esparza, Susan Venable, Thomas D. Horvath, Sigmund J. Haidacher, Kathleen M. Hoch, Anthony M. Haag, Deborah A. Schady, Joseph M. Hyser, Jennifer K. Spinler, James Versalovic
Summary: This study demonstrates that secreted factors from Bifidobacterium dentium can reduce ER stress genes, promote MUC2 secretion, suppress inflammation, and positively modulate the UPR, showing potential benefits for inflammatory bowel diseases.
Article
Physiology
Melinda A. Engevik, Wenly Ruan, Magdalena Esparza, Robert Fultz, Zhongcheng Shi, Kristen A. Engevik, Amy C. Engevik, Faith D. Ihekweazu, Chonnikant Visuthranukul, Susan Venable, Deborah A. Schady, James Versalovic
Summary: This study demonstrates that both L. reuteri secreted factors and its bacterial components can promote dendritic cell maturation and potentially reduce inflammation in acute colitis.
PHYSIOLOGICAL REPORTS
(2021)
Article
Gastroenterology & Hepatology
Anne R. Meyer, Amy C. Engevik, Spencer G. Willet, Janice A. Williams, Yong Zou, Pierre P. Massion, Jason C. Mills, Eunyoung Choi, James R. Goldenring
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2019)