Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 34, Issue -, Pages 77-88Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2017.04.018
Keywords
Bioactive peptides; LC-MS; Gelatin hydrolysate; Angiotensin converting enzyme; Aspergillus niger prolyl endoproteinase (An-PEP); Protein hydrolysate; Spontaneously hypertensive rats
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Funding
- Irish Research Council (IRC)
- National Development Plan, through the Food Institutional Research Measure (FIRM) [11/F/063, 11/F/064, 13/F/467, 13/F/536, 14/F/873]
- Science Foundation Ireland Infrastructure Fund
- Canadian Foundation for Innovation through the Leaders Opportunity Fund
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A targeted enzymatic approach was employed for the generation of angiotensin converting enzyme (ACE) inhibitory/anti-hypertensive peptides. Porcine skin gelatin was hydrolysed with Aspergillus niger prolyl endoproteinase (An-PEP) to produce an hydrolysate with potent ACE inhibitory activity (mean IC50: 220.2 mu g mL(-1)) after 4-h hydrolysis. Peptide identification was achieved by UPLC-ESI-MS/MS. The ACE inhibitory activity of a selection of the identified peptides was determined. The most potent peptide was Met-Gly-Pro with an ACE IC50 of 51.11 +/- 1.14 mu M. Furthermore, oral administration (50 mg/kg body weight) of the hydrolysate to spontaneously hypertensive rats resulted in decreases in systolic and diastolic blood pressure of -28.89 +/- 5.11 and -22.86 +/- 5.65 mm Hg, respectively. Mean arterial pressure and heart rate were also reduced (-25.99 +/- 5.66 mm Hg and 53.63 +/- 17.67 bpm, respectively). The beneficial in vivo effects may be related to the potent C-terminal containing proline ACE-inhibitory peptides in the hydrolysate. (C) 2017 Elsevier Ltd. All rights reserved.
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