Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 31, Issue -, Pages 237-247Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2017.02.003
Keywords
cytotoxicity Functional food; isothiocyanates Raphanus sativus var. caudatus Alef; Thai rat-tailed radish; UHPLC-QToF-MS/MS
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Funding
- Thailand Research Fund through Royal Golden Jubilee Ph.D. Program [PHD/0078/2556]
- Thailand Research Fund [RSA5780017]
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The bioactive compounds of Raphanus sativus var. caudatus Alef and their respective cytotoxicity were identified from (a) 2 crude water and dichloromethane extracts and (b) 5 serially partitioned extracts using dichloromethane. Then, using a bioassay-guided cytotoxicity assay, the extracts were tested against the colon cancer cell line HCT116. Among 2 crude extracts and 5 fractions, the dichloromethane crude extract possessed the greatest in vitro cytotoxicity against HCT116. The dichloromethane crude extract was subjected to flash column liquid chromatography. Only 4 fractions and unfractionated extract were chosen for further analysis by high accuracy mass spectrometry (UHPLC-QToF-MS/MS). Six glucosinolates, 13 isothiocyanates, 5 indoles, 4 flavonoids, 2 alkaloids, 2 thiocyanates, 1 oxazolidine, and 1 dialkyl disulphide were identified. Two compounds were detected for the first time isoalliin and butyl 1-(methylthio)propyl disulphide. Several constituents with anti-cancer activity were identified. This information could inform guidelines for quality control of standard plant extracts for further product development.(C) 2017 Elsevier Ltd. All rights reserved.
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