Gallocatechin gallate (GCG) inhibits 3T3-L1 differentiation and lipopolysaccharide induced inflammation through MAPK and NF-κB signaling

Gallocatechin gallate (GCG), as the epimer of Epigallocatechin gallate (EGCG), the content is low in traditional tea made from Camellia sinensis and C. assamica. However, previous studies found that the content of GCG was almost the same as EGCG in canned and bottled tea drinks. Accumulating evidences indicated that GCG possessed multiple biological activities, but the anti-obesity study of GCG is poorly reported. In this study, the anti-adipogenic activities of GCG were investigated. The results showed that as EGCG, GCG significantly reduced the intracellular lipid droplets and expressions of major adipogenic transcription factors, such as PPAR gamma, SREBP-lc and C/EBP alpha. Further study revealed that the expressions of FAS, ACC, FAT and SCD-1 were decreased in 3T3-L1 cells treated with GCG. GCG treatment also dose dependently decreased the intracellular ROS level, attenuated MAPK pathway activation in 3T3-L1 differentiations. Additionally, GCG decreased the activation of NF-kappa B, and down-regulated the production of IL-6 and MCP-1 induced by LPS. (C) 2017 Elsevier Ltd. All rights reserved.