Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 37, Issue -, Pages 553-563Publisher
ELSEVIER
DOI: 10.1016/j.jff.2017.08.029
Keywords
Flavanols; Evening primrose; Colon cells; NF-kappa B; COX-2; MMP-9
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Funding
- Medical University of Lodz [503/1-156-04/503-01]
- Polish Ministry of Science and Higher Education [NN 312-446-840]
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The present study examines the chemical composition of an evening primrose flavanol preparation (EPFP) and its chemopreventive activity in colon cancer SW-480 cells. EPFP was found to inhibit SW-480 cell viability by 67% vs. controls at 150 mu M catechin equivalents (CE) following 72 h incubation (IC50 = 65 mu M CE). EPFP decreased both NF-kappa B protein expression (33% vs. control at 100 mu M CE) and phosphorylation at serine 529 of the p65 subunit (49% vs. control at 100 mu M CE) in SW-480 cells. Moreover, 100 mu M CE EPFP sup-pressed COX-2 mRNA expression to 23% of control values and protein expression to 78%. In addition, EPFP reduced MMP-9 protein expression to 24% vs. controls at 100 mu M CE. These results provide the first evidence that EPFP reduces NF-kappa B, COX-2, and MMP-9 expression, which may suggest it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards colon cancer cells. (C) 2017 Elsevier Ltd. All rights reserved.
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