Article
Biochemistry & Molecular Biology
Sakshi Kamboj, Akanksha Rajput, Amber Rastogi, Anamika Thakur, Manoj Kumar
Summary: This study developed the Anti-HCV platform using machine learning and QSAR approaches to predict repurposed drugs targeting HCV non-structural proteins. Different machine learning techniques were used to develop predictive models, which were validated through molecular docking. Promising repurposed drugs were identified, which may be useful in antiviral drug development against HCV.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Gastroenterology & Hepatology
Zahra Hasanshahi, Ava Hashempour, Farzane Ghasabi, Javad Moayedi, Zahra Musavi, Behzad Dehghani, Heidar Sharafi, Hassan Joulaei
Summary: In Iranian patients with initial HCV infection, various amino acid residue changes were found in NS5A and NS5B proteins, but no mutations related to treatment resistance were discovered in NS5B. Bioinformatics analysis revealed strong interactions between inhibitors, NS5A and NS5B proteins, and a lack of efficient drug resistance mutations in the analyzed sequences.
BMC GASTROENTEROLOGY
(2021)
Article
Multidisciplinary Sciences
Michael Bobardt, Christina M. Ramirez, Marc M. Baum, Daren Ure, Robert Foster, Philippe A. Gallay
Summary: The combination of NS5A inhibitors and cyclophilin inhibitors has been shown to effectively inhibit HCV replication in vivo, particularly for GT2a and GT3a genotypes. Combining CypI with NS5Ai also prevents viral rebound compared to using NS5Ai alone.
Article
Biology
Katayoun Mehrpour, Seyed Abbas Mirzaei, Amir Savardashtaki, Navid Nezafat, Younes Ghasemi
Summary: The aim of this study was to design a multi-epitope protein from common HCV genotypes in Iran in order to increase the specificity and sensitivity of the diagnosis. By identifying potential epitopes in antigenic proteins and linking the segments, a construct capable of detecting HCV genotypes was successfully designed.
Article
Microbiology
Olaf Isken, Thomas Walther, Luis Wong-Dilworth, Dirk Rehders, Lars Redecke, Norbert Tautz
Summary: The cleavage of Hepatitis C Virus NS2-NS3 is crucial for genome replication and virus assembly, and the activation of NS2(pro) depends on hydrophobic NS3 surface residues and NS2-NS3 surface interactions. In this study, researchers identified two NS2 surface residues that are important for NS2(pro) activation by NS3. These findings provide a basis for understanding the molecular mechanisms of NS2(pro) activation by NS3.
Article
Biochemistry & Molecular Biology
Arthi Venkatesan, Anantha Krishnan Dhanabalan, Selvakumar Rajendran, Shyamala Gowri Shanmugasundharam, Krishnasamy Gunasekaran, J. Febin Prabhu Dass
Summary: Hepatitis C is an infectious disease that leads to liver illnesses. There are no effective vaccines against this virus. Through structure-based docking study, potential compounds that bind well to the HCV protease and polymerase proteins were identified, potentially serving as effective drugs for treating HCV.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Medicine, General & Internal
Nevine Bishai, Walid El Nabawy, Mohamed El Fiki, Mohamed Ibrahim, Nouman El Garem
Summary: This study compared the effectiveness of different treatment regimens for chronic HCV patients and found that the regimen using sofosbuvir, daclatasvir, and ribavirin showed the highest cure rate with the least side effects.
IRISH JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Microbiology
Pooneh Rahimi, Heidar Sharafi, Golnaz Bahramali, FaridehSadat SajadianFard, Nafiseh Sadat Asadi, Seyed Moayed Alavian, Vahid Iranpur Mobarakeh, Seyedeh Zahra Moravej
Summary: In Iranian patients with HCV-1a and HCV-3a infections, baseline amino acid substitutions/RASs were only found in the NS5A region, with no resistance substitutions identified in the NS5B region.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Mohammad Asrar Izhari
Summary: This study aimed to determine the increasing risk of direct-acting antiviral (DAA) resistance due to amino acid substitutions in non-structural proteins of the hepatitis C virus (HCV). Through sequence analysis, the study identified several resistance-associated amino acid substitutions in DAA target proteins. The findings highlight the importance of continuous monitoring and evaluation of DAA resistance in the treatment of HCV.
Article
Chemistry, Multidisciplinary
Asmaa Abo Elgoud Said, Ahmed H. Afifi, Taha F. S. Ali, Mamdouh Nabil Samy, Usama Ramadan Abdelmohsen, Mostafa A. Fouad, Eman Zekry Attia
Summary: Chemical investigation of Aptenia cordifolia roots extract resulted in isolation and identification of eight known compounds. The basic ethyl acetate fraction showed high activity against HCV with an IC50 value of 2.4 mu g mL(-1), and some compounds exhibited strong binding to the active site of NS3/4A helicase.
Article
Cell Biology
Mohd Amir F. Abdullah, Sarah M. M. McWhirter, Zucai Suo
Summary: Hepatitis C virus NS3/NS4A protease complex interacts with host proteins, causing adverse effects on cellular function and immune response. The cleavage of immune factors by NS3/NS4A helps the virus evade innate immunity. NS3/NS4A treatment reduces the kinase activities of IKK alpha and IKK beta, deactivating NF-kappa B-associated innate immune responses. However, it enhances the kinase activities of IKK epsilon and TBK1, facilitating antiviral pathways. After longer cleavage, IKK epsilon and TBK1 gradually lose their kinase activities, leading to the establishment of chronic HCV infection.
Article
Infectious Diseases
Ana Paula de Torres Santos, Vanessa Cristina Martins Silva, Maria Cassia Mendes-Correa, Marcilio Figueiredo Lemos, Fernanda de Mello Malta, Rubia Anita Ferraz Santana, Gregorio Tadeu Fernando Dastoli, Vanessa Fusco Duarte de Castro, Joao Renato Rebello Pinho, Regina Celia Moreira
Summary: This study found important NS5A resistance-associated substitutions in samples from chronic HCV patients in Sao Paulo, indicating resistance to some DAAs. No NS5B resistance-associated substitutions were found in the samples.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Chemistry, Medicinal
Zhilan Zhou, Jifa Zhang, Enda Zhou, Changyu Ren, Jiaxing Wang, Yuxi Wang
Summary: HCV infection is a global health problem, and developing small-molecule inhibitors targeting NS5B RdRp is of great value. Non-nucleoside inhibitors have more flexible structures and simpler mechanisms compared to nucleoside inhibitors.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Tomasz. M. Karpinski, Marcin Ozarowski, Pedro. J. Silva, Mark Stasiewicz, Rahat Alam, Abdus Samad
Summary: This study aimed to test the in silico activity of 1893 terpenes against HCV NS5B polymerase. Nine terpenes were identified as potential effective competitive inhibitors, forming stable interactions with the active site region.
Article
Biochemistry & Molecular Biology
Simon Gonzalez, Gabriela Brzuska, Abdelhakim Ouarti, Florian Gallier, Carmen Solarte, Angelique Ferry, Jacques Uziel, Ewelina Krol, Nadege Lubin-Germain
Summary: The compound 2 carrying an N-hydroxy carboxamide function exhibited the most inhibitory activities against both Zika virus (ZIKV) and hepatitis C virus (HCV), suggesting it could be a potential candidate for the development of new anti-HCV and anti-ZIKV drugs.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)